[Optimization of the method to cultivate NK cells from abandoned white cells].

OBJECTIVE: Using abandoned white cells separated from preparation of blood products to cultivate NK cells in vitro, and to optimize the method of cultivation of allogeneic NK cells for clinical application. METHODS: Abandoned white cells separated from blood production were collected from 15 healthy donors. PBMCs were isolated from the abandoned white cells and cultured for 17 days using culture bottles as previously coated antibodies (group CD3 mAb was coated with CD3 mAb, group CD 16mAb was coated with CD16mAb, and group CD3 mAb+ CD16 mAb was coated with CD3 mAb and CD16 mAb). Flow cytometry was used to determine the ratio of CD3(-)CD56(+) cells, expression of activated cell surface receptors, and secretion of IFN-γ. The anti-tumor cytotoxicity against K562 and Raji cells was determined using LDH cytotoxicity assay and flow cytometry. RESULTS: After expansion for 17 days, the proportions of CD3(-)CD56(+) cells was (15.19±12.22)% in the group CD3 mAb, (83.63±10.63)% in the group CD16 mAb, (49.40±12.64)% in the group CD3 mAb+ CD16 mAb, and it was (16.34±10.51)% before expansion. The total number of NK cells was more than 10(9). The expression ratios of NK cell surface activated receptors NKp30 and NKp46 were significantly increased, while that of the NKG2D was not significantly changed. The NK cells after expansion showed high cytotoxicity activity against K562 cells, reaching up to(76.97±3.16)% when effector-cell-to-target-cell ratio (E∶T ratio) was 40∶1. CONCLUSIONS: NK cells can be obtained from abandoned white cells after cultivation for 17 days, with a purity up to 90% and total cell number of more than 10(9). Their activity was reinforced, the anti-tumor cytotoxicity activity was increased, and may meet the standard of clinical therapeutic application.

The electrophysiological effects of alcoholized rhubarb solution in feline heart in vivo.

The effects of Chinese medicine, alcoholized rhubarb, on cardiac electrophysiology and contractile force were studied by using monophasic action potential (MAP) techniques with an improved pressure-electrical transducer epicardial electrode and/or an endocardial pressure-contact electrode catheter in 27 feline hearts in vivo. The results of the experiments were as follows: 1) Alcoholized rhubarb solution (1.5 g/kg) caused a greater increase in the MAP amplitude (from 18.8 +/- 0.62 mV to 26.7 +/- 3.87 mV, p < 0.01), in the MAP maximum rate of rise of phase 0 (from 1.7 +/- 0.13 V/sec to 2.6 +/- 0.43, p < 0.01), and in the MAP duration at 50% and 90% repolarization (from 194.3 +/- 14.00 msec to 216.3 +/- 6.30 msec and from 238.8 +/- 21.41 msec to 280.5 +/- 14.68 msec, respectively, p < 0.01), and a decrease in the heart rate (from 130.8 +/- 12.10 bpm to 110.8 +/- 10.35 bpm, p < 0.01) in the epicardial monophasic action potentials after rhubarb solution administration for 1 min. 2) The ventricular contractile force of the feline heart in vivo was also significantly increased (11.1 +/- 0.52 g to 14.0 +/- 1.41 g, p < 0.01) by rhubarb solution. 3) A dose-dependent relation of alcoholized rhubarb solution was found and indicated that lower doses (1.0-1.5 g/kg) had an excitatory effect, while a higher dose (2.0 g/kg) had inhibitory or toxic effects on the intact feline heart. The present study provides evidence that Chinese rhubarb solution has electrophysiological effects on the feline heart in vivo.

[Effects of rhubarb on electrical and contractive activities of the isolated intestine in rats].

The results indicate that electrical activity of colon is obviously excited by rhubarb (Rheum tanguticum). The characteristics of excitement is cybotaxis discharge. It has been found that the frequency and amplitude of spike potential increase obviously, and the contraction enhances. Spike potential frequency of duodenum excited by rhubarb is increased but that of jejunum is hardly excited. It has also been found that the exciting effect of rhubarb on colon can be blocked by atropine.

[The quantitative and qualitative analysis fro submandibular gland of human being.].

30 cases of submandibular gland tissue were determined quantitatively in this article.It is noticed that there was no great change during and after the birth .But at the adolesence stage,the data began to change was steady in adult state.At pressenium,the change speed of mean was faster.Observation under microscope:the submandibular gland in 7-8 month old embryo was developed basically and similar to newborn.It had good blood supply and secrete function.Adipose tissue,which is important indication of changed with age in saliva gland,did not exit in submandibular gland of human being.In addition.it is normal that there were a few lymphatic cells and plasmocytes with secrete SIgA in the gland and is relating with protective principle of normal environment.

Pulmonary artery involvement in aortoarteritis: an angiographic study.

Angiographic findings of 47 patients with pulmonary artery involvement (PAI) in aortoarteritis are described together with the diagnostic evaluation of PAI and its clinical implications. Pulmonary arteriography was obtained by intravenous digital subtraction angiography in 133 patients with aortoarteritis; 45 (33.8%) were found to have PAI. Two patients with PAI, studied by conventional arteriography, are also included. Stenosis and/or occlusion of segmental and/or lobar pulmonary arteries, and subsegmental branches, were the basic angiographic findings in PAI. Pulmonary artery branches in the upper lobes were more commonly affected than those in the lower and middle (lingula) lobes. Bilateral lesions were more common than unilateral ones. Single lobar and segmental lesions were quite rare. Dilatation was uncommon. No main pulmonary artery involvement was detected. Pulmonary artery hypertension, a late complication of patients with PAI, was revealed in 9 of the 46 patients of the present series. If it occurs, PAI becomes a factor effecting the patient's clinical course and prognosis.