RESUMO
A latex agglutination test to detect urinary antigens for visceral leishmaniasis (VL) was studied. In 204 patients with suspected VL, KAtex had a sensitivity of 95.2% with good agreement with microscopy smears but poor agreement with 4 different serology tests. It was also positive in 2 confirmed VL cases co-infected with HIV. In all K4tex-positive confirmed cases actively followed up after treatment, the test became negative 1 month after completion of treatment. While IC4tex had a specificity of 100% in healthy endemic and non-endemic controls, the direct agglutination test (DAT) was positive in 14% of the KAtex-negative healthy endemic controls. KAtex is a simple addition to the diagnostics of VL particularly at field level and as a complementary test for the diagnosis of VL in smear-negative cases with positive DAT results.
Assuntos
Antígenos de Protozoários/urina , Testes de Fixação do Látex/métodos , Leishmania donovani/imunologia , Leishmaniose Visceral/diagnóstico , Leishmaniose Visceral/urina , Adolescente , Adulto , Animais , Estudos de Casos e Controles , Criança , Pré-Escolar , Doenças Endêmicas/estatística & dados numéricos , Ensaio de Imunoadsorção Enzimática/normas , Feminino , Técnica Indireta de Fluorescência para Anticorpo/normas , Humanos , Immunoblotting/normas , Lactente , Testes de Fixação do Látex/normas , Leishmaniose Visceral/epidemiologia , Leishmaniose Visceral/imunologia , Masculino , Pessoa de Meia-Idade , Parasitologia/normas , Prognóstico , Sensibilidade e Especificidade , Sudão/epidemiologiaRESUMO
A latex agglutination test to detect urinary antigens for visceral leishmaniasis [VL] was studied. In 204 patients with suspected VL, KAtex had a sensitivity of 95.2% with good agreement with microscopy smears but poor agreement with 4 different serology tests. It was also positive in 2 confirmed VL cases co-infected with HIV. In all KAtex-positive confirmed cases actively followed up after treatment, the test became negative 1 month after completion of treatment. While KAtex had a specificity of 100% in healthy endemic and non-endemic controls, the direct agglutination test [DAT] was positive in 14% of the KAtex-negative healthy endemic controls. KAtex is a simple addition to the diagnostics of VL particularly at field level and as a complementary test for the diagnosis of VL in smear-negative cases with positive DAT results