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1.
Proc Natl Acad Sci U S A ; 120(23): e2212394120, 2023 06 06.
Artigo em Inglês | MEDLINE | ID: mdl-37252991

RESUMO

The perception of pain is a multidimensional sensory and emotional/affective experience arising from distributed brain activity. However, the involved brain regions are not specific for pain. Thus, how the cortex distinguishes nociception from other aversive and salient sensory stimuli remains elusive. Additionally, the resulting consequences of chronic neuropathic pain on sensory processing have not been characterized. Using in vivo miniscope calcium imaging with cellular resolution in freely moving mice, we elucidated the principles of nociceptive and sensory coding in the anterior cingulate cortex, a region essential for pain processing. We found that population activity, not single-cell responses, allowed discriminating noxious from other sensory stimuli, ruling out the existence of nociception-specific neurons. Additionally, single-cell stimulus selectivity was highly dynamic over time, but stimulus representation at the population level remained stable. Peripheral nerve injury-induced chronic neuropathic pain led to dysfunctional encoding of sensory events by exacerbation of responses to innocuous stimuli and impairment of pattern separation and stimulus classification, which were restored by analgesic treatment. These findings provide a novel interpretation for altered cortical sensory processing in chronic neuropathic pain and give insights into the effects of systemic analgesic treatment in the cortex.


Assuntos
Giro do Cíngulo , Neuralgia , Humanos , Camundongos , Animais , Giro do Cíngulo/diagnóstico por imagem , Nociceptividade/fisiologia , Encéfalo , Nociceptores
2.
J Neurosci ; 35(41): 13975-88, 2015 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-26468198

RESUMO

Adolescence is characterized by drastic behavioral adaptations and comprises a particularly vulnerable period for the emergence of various psychiatric disorders. Growing evidence reveals that the pathophysiology of these disorders might derive from aberrations of normal neurodevelopmental changes in the adolescent brain. Understanding the molecular underpinnings of adolescent behavior is therefore critical for understanding the origin of psychopathology, but the molecular mechanisms that trigger adolescent behavior are unknown. Here, we hypothesize that the cannabinoid type-1 receptor (CB1R) may play a critical role in mediating adolescent behavior because enhanced endocannabinoid (eCB) signaling has been suggested to occur transiently during adolescence. To study enhanced CB1R signaling, we introduced a missense mutation (F238L) into the rat Cnr1 gene that encodes for the CB1R. According to our hypothesis, rats with the F238L mutation (Cnr1(F238L)) should sustain features of adolescent behavior into adulthood. Gain of function of the mutated receptor was demonstrated by in silico modeling and was verified functionally in a series of biochemical and electrophysiological experiments. Mutant rats exhibit an adolescent-like phenotype during adulthood compared with wild-type littermates, with typical high risk/novelty seeking, increased peer interaction, enhanced impulsivity, and augmented reward sensitivity for drug and nondrug reward. Partial inhibition of CB1R activity in Cnr1(F238L) mutant rats normalized behavior and led to a wild-type phenotype. We conclude that the activity state and functionality of the CB1R is critical for mediating adolescent behavior. These findings implicate the eCB system as an important research target for the neuropathology of adolescent-onset mental health disorders. SIGNIFICANCE STATEMENT: We present the first rodent model with a gain-of-function mutation in the cannabinoid type-1 receptor (CB1R). Adult mutant rats exhibit an adolescent-like phenotype with typical high risk seeking, impulsivity, and augmented drug and nondrug reward sensitivity. Adolescence is a critical period for suboptimal behavioral choices and the emergence of neuropsychiatric disorders. Understanding the basis of these disorders therefore requires a comprehensive knowledge of how adolescent neurodevelopment triggers behavioral reactions. Our behavioral observations in adult mutant rats, together with reports on enhanced adolescent CB1R signaling, suggest a pivotal role for the CB1R in an adolescent brain as an important molecular mediator of adolescent behavior. These findings implicate the endocannabinoid system as a notable research target for adolescent-onset mental health disorders.


Assuntos
Comportamento do Adolescente/fisiologia , Comportamento Animal/fisiologia , Receptor CB1 de Canabinoide/metabolismo , Adolescente , Fatores Etários , Animais , Comportamento Animal/efeitos dos fármacos , Encéfalo/diagnóstico por imagem , Encéfalo/efeitos dos fármacos , Encéfalo/crescimento & desenvolvimento , Antagonistas de Receptores de Canabinoides/farmacologia , Cocaína/administração & dosagem , Corpo Estriado/citologia , Endocanabinoides/metabolismo , Endocanabinoides/farmacologia , Comportamento Exploratório/efeitos dos fármacos , Comportamento Exploratório/fisiologia , Guanosina 5'-O-(3-Tiotrifosfato)/farmacocinética , Humanos , Técnicas In Vitro , Masculino , Aprendizagem em Labirinto/efeitos dos fármacos , Aprendizagem em Labirinto/fisiologia , Modelos Animais , Mutação/genética , Cintilografia , Ratos , Ratos Endogâmicos F344 , Ratos Transgênicos , Receptor CB1 de Canabinoide/genética , Assunção de Riscos , Comportamento Social , Isótopos de Enxofre/farmacocinética
3.
Neuroscience ; 523: 91-104, 2023 07 15.
Artigo em Inglês | MEDLINE | ID: mdl-37236391

RESUMO

Maladaptive neuronal plasticity is a main mechanism for the development and maintenance of pathological pain. Affective, motivational and cognitive deficits that are comorbid with pain involve cellular and synaptic modifications in the anterior cingulate cortex (ACC), a major brain mediator of pain perception. Here we use a model of neuropathic pain (NP) in male mice and ex-vivo electrophysiology to investigate whether layer 5 caudal ACC (cACC) neurons projecting to the dorsomedial striatum (DMS), a critical region for motivational regulation of behavior, are involved in aberrant neuronal plasticity. We found that while the intrinsic excitability of cortico-striatal cACC neurons (cACC-CS) was preserved in NP animals, excitatory postsynaptic potentials (EPSP) induced after stimulation of distal inputs were enlarged. The highest synaptic responses were evident both after single stimuli and in each of the EPSP that compose responses to trains of stimuli, and were accompanied by increased synaptically-driven action potentials. EPSP temporal summation was intact in ACC-CS neurons from NP mice, suggesting that the plastic changes were not due to alterations in dendritic integration but rather through synaptic mechanisms. These results demonstrate for the first time that NP affects cACC neurons that project to the DMS and reinforce the notion that maladaptive plasticity of the cortico-striatal pathway may be a key factor in sustaining pathological pain.


Assuntos
Giro do Cíngulo , Neuralgia , Masculino , Camundongos , Animais , Giro do Cíngulo/fisiologia , Neurônios/fisiologia , Neuralgia/metabolismo , Potenciais de Ação/fisiologia , Corpo Estriado , Plasticidade Neuronal/fisiologia
4.
Proc Natl Acad Sci U S A ; 105(23): 8124-9, 2008 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-18523020

RESUMO

Coordinated near-threshold depolarized states in cortical and striatal neurons may contribute to form functionally segregated channels of information processing. Recent anatomical studies have identified pathways that could support spiraling interactions across corticostriatal channels, but a functional outcome of such spiraling remains to be identified. Here, we examined whether plateau depolarizations (UP states) in striatal neurons relate better to active epochs in local field potentials recorded from closely related cortical areas than to those recorded in less-related cortical areas. Our results show that, in anesthetized rats, the coordination between cortical areas and striatal regions obeys a mediolateral gradient and keeps track of slow wave trajectory across the neocortex. Moreover, activity in one cortical area induced phase advances in UP state onset and phase delays in UP state termination in nonmatching striatal regions, reflecting the existence of functional connections that could encode large-scale interactions between corticostriatal channels as subthreshold influences on striatal projection neurons.


Assuntos
Canais Iônicos/fisiologia , Potenciais da Membrana/fisiologia , Neostriado/fisiologia , Anestesia , Animais , Eletrodos , Ativação do Canal Iônico/efeitos dos fármacos , Masculino , Potenciais da Membrana/efeitos dos fármacos , Córtex Motor/efeitos dos fármacos , Córtex Motor/fisiologia , Neocórtex/efeitos dos fármacos , Neocórtex/fisiologia , Neostriado/efeitos dos fármacos , Neurônios/efeitos dos fármacos , Neurônios/fisiologia , Ratos , Ratos Sprague-Dawley , Uretana/administração & dosagem , Uretana/farmacologia
5.
Sci Rep ; 11(1): 24240, 2021 12 20.
Artigo em Inglês | MEDLINE | ID: mdl-34930957

RESUMO

Neuropathic pain induces changes in neuronal excitability and synaptic connectivity in deep layers of the anterior cingulate cortex (ACC) that play a central role in the sensory, emotional and affective consequences of the disease. However, how this impacts ACC in vivo activity is not completely understood. Using a mouse model, we found that neuropathic pain caused an increase in ACC in vivo activity, as measured by the indirect activity marker c-Fos and juxtacellular electrophysiological recordings. The enhanced firing rate of ACC neurons in lesioned animals was based on a change in the firing pattern towards bursting activity. Despite the proportion of ACC neurons recruited by noxious stimuli was unchanged during neuropathic pain, responses to noxious stimuli were characterized by increased bursting. Thus, this change in coding pattern may have important implications for the processing of nociceptive information in the ACC and could be of great interest to guide the search for new treatment strategies for chronic pain.


Assuntos
Giro do Cíngulo/fisiopatologia , Ketamina/farmacologia , Neuralgia/fisiopatologia , Potenciais de Ação , Anestesia/métodos , Animais , Dor Crônica , Eletrofisiologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Neurônios/metabolismo , Proteínas Proto-Oncogênicas c-fos/metabolismo , Nervo Isquiático/efeitos dos fármacos , Transmissão Sináptica
6.
Neuropsychopharmacology ; 41(9): 2192-205, 2016 08.
Artigo em Inglês | MEDLINE | ID: mdl-26612422

RESUMO

The type 1 cannabinoid receptor (CB1) modulates numerous neurobehavioral processes and is therefore explored as a target for the treatment of several mental and neurological diseases. However, previous studies have investigated CB1 by targeting it globally, regardless of its two main neuronal localizations on glutamatergic and GABAergic neurons. In the context of cocaine addiction this lack of selectivity is critical since glutamatergic and GABAergic neuronal transmission is involved in different aspects of the disease. To determine whether CB1 exerts different control on cocaine seeking according to its two main neuronal localizations, we used mutant mice with deleted CB1 in cortical glutamatergic neurons (Glu-CB1) or in forebrain GABAergic neurons (GABA-CB1). In Glu-CB1, gene deletion concerns the dorsal telencephalon, including neocortex, paleocortex, archicortex, hippocampal formation and the cortical portions of the amygdala. In GABA-CB1, it concerns several cortical and non-cortical areas including the dorsal striatum, nucleus accumbens, thalamic, and hypothalamic nuclei. We tested complementary components of cocaine self-administration, separating the influence of primary and conditioned effects. Mechanisms underlying each phenotype were explored using in vivo microdialysis and ex vivo electrophysiology. We show that CB1 expression in forebrain GABAergic neurons controls mouse sensitivity to cocaine, while CB1 expression in cortical glutamatergic neurons controls associative learning processes. In accordance, in the nucleus accumbens, GABA-CB1 receptors control cocaine-induced dopamine release and Glu-CB1 receptors control AMPAR/NMDAR ratio; a marker of synaptic plasticity. Our findings demonstrate a critical distinction of the altered balance of Glu-CB1 and GABA-CB1 activity that could participate in the vulnerability to cocaine abuse and addiction. Moreover, these novel insights advance our understanding of CB1 neuropathophysiology.


Assuntos
Encéfalo/efeitos dos fármacos , Cocaína/administração & dosagem , Comportamento de Procura de Droga , Neurônios GABAérgicos/efeitos dos fármacos , Ácido Glutâmico/fisiologia , Neurônios/efeitos dos fármacos , Receptor CB1 de Canabinoide/fisiologia , Animais , Encéfalo/metabolismo , Encéfalo/fisiologia , Sinais (Psicologia) , Dopamina/metabolismo , Neurônios GABAérgicos/fisiologia , Camundongos , Camundongos Knockout , Neurônios/fisiologia , Núcleo Accumbens/efeitos dos fármacos , Núcleo Accumbens/metabolismo , Receptor CB1 de Canabinoide/genética , Receptor CB1 de Canabinoide/metabolismo , Receptores de AMPA/metabolismo , Receptores de N-Metil-D-Aspartato/metabolismo , Reforço Psicológico , Autoadministração
7.
Science ; 343(6166): 94-8, 2014 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-24385629

RESUMO

Pregnenolone is considered the inactive precursor of all steroid hormones, and its potential functional effects have been largely uninvestigated. The administration of the main active principle of Cannabis sativa (marijuana), Δ(9)-tetrahydrocannabinol (THC), substantially increases the synthesis of pregnenolone in the brain via activation of the type-1 cannabinoid (CB1) receptor. Pregnenolone then, acting as a signaling-specific inhibitor of the CB1 receptor, reduces several effects of THC. This negative feedback mediated by pregnenolone reveals a previously unknown paracrine/autocrine loop protecting the brain from CB1 receptor overactivation that could open an unforeseen approach for the treatment of cannabis intoxication and addiction.


Assuntos
Encéfalo/efeitos dos fármacos , Cannabis/toxicidade , Dronabinol/toxicidade , Pregnenolona/administração & dosagem , Pregnenolona/metabolismo , Receptor CB1 de Canabinoide/agonistas , Receptor CB1 de Canabinoide/antagonistas & inibidores , Animais , Encéfalo/metabolismo , Antagonistas de Receptores de Canabinoides/administração & dosagem , Masculino , Abuso de Maconha/tratamento farmacológico , Camundongos , Camundongos Endogâmicos C57BL , Ratos , Ratos Sprague-Dawley , Ratos Wistar
8.
J Physiol Paris ; 106(1-2): 40-6, 2012 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-21767642

RESUMO

Up states are a hallmark of striatal physiology. Spontaneous activity in the thalamo-cortical network drives robust plateau depolarizations in the medium spiny projection neurons of the striatum. Medium spiny neuron firing is only possible during up states and is very tightly regulated by dopamine and NMDA receptors. In a rat model of Parkinson's disease the medium spiny neurons projecting to the globus pallidus (indirect pathway) show more depolarized up states and increased firing. This is translated into abnormal patterns of synchronization between the globus pallidus and frontal cortex, which are believed to underlie the symptoms of Parkinson's disease. Here we review our work in the field and propose a mechanism through which the lack of D2 receptor stimulation in the striatum allows the establishment of fixed routes of information flow in the cortico-striato-pallidal network.


Assuntos
Gânglios da Base/fisiologia , Relógios Biológicos/fisiologia , Corpo Estriado/fisiologia , Ativação do Canal Iônico/fisiologia , Animais , Gânglios da Base/efeitos dos fármacos , Corpo Estriado/citologia , Fármacos Atuantes sobre Aminoácidos Excitatórios/farmacologia , Humanos , Neurônios/efeitos dos fármacos , Neurônios/fisiologia , Doença de Parkinson/metabolismo , Doença de Parkinson/patologia , Receptores de Dopamina D2/metabolismo , Receptores de N-Metil-D-Aspartato/metabolismo
9.
Science ; 328(5986): 1709-12, 2010 Jun 25.
Artigo em Inglês | MEDLINE | ID: mdl-20576893

RESUMO

Chronic exposure to drugs of abuse induces countless modifications in brain physiology. However, the neurobiological adaptations specifically associated with the transition to addiction are unknown. Cocaine self-administration rapidly suppresses long-term depression (LTD), an important form of synaptic plasticity in the nucleus accumbens. Using a rat model of addiction, we found that animals that progressively develop the behavioral hallmarks of addiction have permanently impaired LTD, whereas LTD is progressively recovered in nonaddicted rats maintaining a controlled drug intake. By making drug seeking consistently resistant to modulation by environmental contingencies and consequently more and more inflexible, a persistently impaired LTD could mediate the transition to addiction.


Assuntos
Comportamento Aditivo , Transtornos Relacionados ao Uso de Cocaína/fisiopatologia , Depressão Sináptica de Longo Prazo , Núcleo Accumbens/fisiopatologia , Animais , Cocaína/administração & dosagem , Modelos Animais de Doenças , Ácido Glutâmico/metabolismo , Ratos , Ratos Sprague-Dawley , Receptores de Glutamato Metabotrópico/metabolismo , Receptores de N-Metil-D-Aspartato/metabolismo , Autoadministração , Transmissão Sináptica
10.
J Neurophysiol ; 101(5): 2516-27, 2009 May.
Artigo em Inglês | MEDLINE | ID: mdl-19244354

RESUMO

Precise control of synaptic strength is critical for maintaining accurate network activity and normal brain functions. Several major brain diseases are related to synaptic alterations in the adult brain. Detailed descriptions of the normal physiological properties of adult synapses are scarce, mainly because of the difficulties in performing whole cell patch-clamp recording in brain slices from adult animals. Here we present the portrait of excitatory synapses and intrinsic properties of medium spiny neurons (MSNs) of the nucleus accumbens (NAc), a central structure of the mesocorticolimbic system, from youth (P14) to adulthood (P120). We found that intrinsic neuronal excitability decreased over development, mainly due to an enhancement of potassium conductance and the consequent reduction in membrane resistance. The ratio between paired-pulse synaptic responses was similar in juvenile, adolescent, and adult MSNs, suggesting that the probability of neurotransmitter release was unaltered. alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR)-mediated excitatory postsynaptic currents (EPSCs) decayed more slowly in adult MSN. In contrast, the kinetic properties and the subunit composition of N-methyl-d-aspartate receptor (NMDAR)-mediated EPSC in the NAc were conserved from youth to adulthood. Changes in synaptic strength were estimated from the ratio of AMPAR to NMDAR evoked and spontaneous EPSCs (AMPAR/NMDAR ratio). Although both AMPAR and NMDAR EPSCs decreased over development, there was an increase of the AMPAR/NMDAR ratio that was linked to changes in NMDAR EPSC. Furthermore, distribution of the AMPAR/NMDAR ratio was more heterogeneous in MSNs from adults, suggesting that synaptic strength is continuously refined during life.


Assuntos
Neurônios/fisiologia , Núcleo Accumbens/citologia , Núcleo Accumbens/crescimento & desenvolvimento , Transmissão Sináptica/fisiologia , Fatores Etários , Análise de Variância , Animais , Animais Recém-Nascidos , Biofísica , Cloreto de Cálcio/farmacologia , Estimulação Elétrica/métodos , Antagonistas de Aminoácidos Excitatórios/farmacologia , Potenciais Pós-Sinápticos Excitadores/efeitos dos fármacos , Potenciais Pós-Sinápticos Excitadores/fisiologia , Técnicas In Vitro , Masculino , Potenciais da Membrana/efeitos dos fármacos , Potenciais da Membrana/fisiologia , Neurônios/efeitos dos fármacos , Técnicas de Patch-Clamp/métodos , Piperidinas/farmacologia , Quinoxalinas/farmacologia , Ratos , Ratos Sprague-Dawley , Receptores de AMPA/antagonistas & inibidores , Receptores de AMPA/fisiologia , Receptores de N-Metil-D-Aspartato/antagonistas & inibidores , Receptores de N-Metil-D-Aspartato/fisiologia
11.
J Physiol ; 577(Pt 1): 97-113, 2006 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-16931555

RESUMO

In vivo, cortical neurons and striatal medium spiny neurons (MSN) display robust subthreshold depolarizations (Up states) during which they are enabled to fire action potentials. In the cortex, Up states are believed to occur simultaneously in a neuronal ensemble and to be sustained by local network interactions. It is known that MSN are impelled into the Up state by extra-striatal (primarily cortical) inputs, but the mechanisms that sustain and determine the end of striatal Up states are still debated. Furthermore, it has not been established if brisk perturbations of ongoing cortical oscillations alter rhythmic transitions between Up and Down states in striatal neurons. Here we report that MSN Up states terminate abruptly when persistent activity in cortical ensembles providing afferents to a given striatal region is turned off by local electrical stimulation or ends spontaneously. In addition, we found that phase perturbations in MSN membrane potential slow oscillations induced by cortical stimulation replicate the stimulus-induced dynamics of spiking activity in cortical ensembles. Overall, these results suggest that striatal Up states are single-cell subthreshold representations of episodes of persistent spiking in cortical ensembles. A precise spatial and temporal alignment between episodes of cortical persistent activity and striatal Up states would allow MSN to detect specific cortical inputs embedded within a more general cortical signal.


Assuntos
Potenciais de Ação/fisiologia , Relógios Biológicos/fisiologia , Córtex Cerebral/fisiologia , Corpo Estriado/fisiologia , Estimulação Elétrica/métodos , Potenciais Evocados/fisiologia , Inibição Neural/fisiologia , Adaptação Fisiológica/fisiologia , Animais , Masculino , Ratos , Ratos Sprague-Dawley
12.
J Physiol ; 543(Pt 2): 577-89, 2002 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-12205191

RESUMO

In anaesthetised animals, the very negative resting membrane potential of striatal spiny neurones (down state) is interrupted periodically by depolarising plateaux (up states) which are probably driven by excitatory input. In the absence of active synaptic input, as occurs in vitro, potassium currents hold the membrane potential of striatal spiny neurones in the down state. Because striatal spiny neurones fire action potentials only during the up state, these plateau depolarisations have been perceived as enabling events that allow information processing through cerebral cortex-basal ganglia circuits. Recent studies have demonstrated that the robust membrane potential fluctuation of spiny neurones is strongly correlated to the slow electroencephalographic rhythms that are typical of slow wave sleep and anaesthesia. To further understand the impact of cortical activity states on striatal function, we studied the membrane potential of striatal neurones during cortical desynchronised states. Simultaneous in vivo recordings of striatal neurones and the electrocorticogram in urethane-anaesthetised rats revealed that rhythmic alternation between up and down states was disrupted during episodes of spontaneous or induced cortical desynchronisation. Instead of showing robust two-state fluctuations, the membrane potential of striatal neurones displayed a persisting depolarised state with fast, low-amplitude modulations. Spiny neurones remained in this persistent up state until the cortex resumed ~1 Hz synchronous activity. Most of the recorded neurones exhibited a low firing probability, irrespective of the cortical activity state. Time series analysis failed to reveal significant correlations between the membrane potential of striatal neurones and the desynchronised electrocorticogram. Our results suggest that during cortical desynchronisation continuous uncorrelated excitatory input sustains the membrane potential of striatal neurones in a persisting depolarised state, but that substantial additional input is necessary to impel the neurones to threshold. Our data support that the prevailing cortical activity state determines the duration of the enabling depolarising events that take place in striatal spiny neurones.


Assuntos
Corpo Estriado/fisiologia , Sincronização Cortical , Neurônios/fisiologia , Anestesia , Animais , Corpo Estriado/citologia , Lobo Frontal/citologia , Lobo Frontal/fisiologia , Masculino , Potenciais da Membrana/fisiologia , Vias Neurais , Ratos , Ratos Sprague-Dawley
13.
Eur J Neurosci ; 17(5): 1046-52, 2003 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-12653980

RESUMO

A high proportion of neurons in the basal ganglia display rhythmic burst firing after chronic nigrostriatal lesions. For instance, the periodic bursts exhibited by certain striatal and subthalamic nucleus neurons in 6-hydroxydopamine-lesioned rats seem to be driven by the approximately 1 Hz high-amplitude rhythm that is prevalent in the cerebral cortex of anaesthetized animals. Because the striatum and subthalamic nucleus are the main afferent structures of the substantia nigra pars reticulata, we examined the possibility that the low-frequency modulations (periodic bursts) that are evident in approximately 50% nigral pars reticulata neurons in the parkinsonian condition were also coupled to this slow cortical rhythm. By recording the frontal cortex field potential simultaneously with single-unit activity in the substantia nigra pars reticulata of anaesthetized rats, we proved the following. (i) The firing of nigral pars reticulata units from sham-lesioned rats is not coupled to the approximately 1 Hz frontal cortex slow oscillation. (ii) Approximately 50% nigral pars reticulata units from 6-hydroxydopamine-lesioned rats oscillate synchronously with the approximately 1 Hz cortical rhythm, with the cortex leading the substantia nigra by approximately 55 ms; the remaining approximately 50% nigral pars reticulata units behave as the units recorded from sham-lesioned rats. (iii) Periodic bursting in nigral pars reticulata units from 6-hydroxydopamine-lesioned rats is disrupted by episodes of desynchronization of cortical field potential activity. Our results strongly support that nigrostriatal lesions promote the spreading of low-frequency cortical rhythms to the substantia nigra pars reticulata and may be of outstanding relevance for understanding the pathophysiology of Parkinson's disease.


Assuntos
Sincronização Cortical , Vias Neurais/fisiologia , Neurônios/fisiologia , Substância Negra/fisiologia , Potenciais de Ação/efeitos dos fármacos , Potenciais de Ação/fisiologia , Adrenérgicos/farmacologia , Animais , Gânglios da Base/fisiologia , Modelos Animais de Doenças , Estimulação Elétrica , Masculino , Vias Neurais/efeitos dos fármacos , Neurônios/efeitos dos fármacos , Oxidopamina/farmacologia , Transtornos Parkinsonianos/fisiopatologia , Ratos , Ratos Sprague-Dawley , Substância Negra/efeitos dos fármacos
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