RESUMO
In many digital X-ray imaging systems, although air kerma on a surface of each detector is used, a standardized dose index called an exposure index (EI) has been proposed by the IEC, which is expected to be utilized for dose management. In clinical practices, EI is effectively utilized using a deviation index (DI), which is a deviation between a target EI (EIT) set for each imaging region and an EIT of the acquired image. However, an important issue in clinical uses of EI is a suppression of excessive doses. It is difficult to achieve a reliable reduction in exposure doses by indicating DI. In this study, physical image characteristics of detectors, visual detectability by charts, and observer experiments using a chest phantom were examined to determine upper (DImax) and lower (DImin) limits of the EIT and DI to achieve a reliable dose reduction in chest examinations. As the result, the tolerance ranges indicated by DImax and DImin, which were set based on the results of physical and visual evaluations, proved to be almost consistent with the distribution of EI values in 735 clinical images taken with a photo-timer control in real clinical practices.
Assuntos
Intensificação de Imagem Radiográfica , Tórax , Intensificação de Imagem Radiográfica/métodos , Imagens de Fantasmas , Doses de RadiaçãoRESUMO
Studies on color vision in invertebrates have focused primarily on insect visual pigments, with little attention given to crustacean visual pigments. None of the blue-green-, blue-, or ultraviolet (UV)-sensitive-opsins have been identified in crustaceans. In addition, the discussion of visual pigments has been limited to long-wavelength-sensitive opsins in Pancrustacea. Here, we focused on Branchiopoda (Crustacea), which is a sister group of Hexapoda including insects. In the tadpole shrimp Triops granarius, the visual pigment chromophore was retinal. Multiple opsins were isolated from each of three branchiopod species, T. granarius, Triops longicaudatus, and the fairy shrimp Branchinella kugenumaensis (five, five, and four opsins from these species, respectively). Phylogenetic analyses and the presence of a lysine residue corresponding to position 90 in bovine rhodopsin suggested that three of the branchiopod opsins comprise UV-sensitive pigments. In addition, the phylogenetic relationships between insect and branchiopod UV-sensitive opsins revealed that the divergence of blue- and UV-sensitive pigments predates the Branchiopoda and Insecta divergence. The other branchiopod opsins show distant relationships to other known insect opsins and form novel clusters. The present results strongly suggest that the ancestral arthropod of the Chelicerata-Pancrustacea lineages possessed at least four types of opsins. The ancestors of Pancrustacea and the Insecta-Branchiopoda lineages possessed at least five and six types of opsins, respectively. Our results suggest that in the evolutionary process associated with each lineage, several opsins appeared and diversified with repeated gene duplication, of which some have been lost in some taxa.
Assuntos
Crustáceos/genética , Evolução Molecular , Opsinas/genética , Animais , Artrópodes/genética , Crustáceos/classificação , Olho/química , Japão , Filogenia , RNA Mensageiro/genética , Pigmentos da Retina/genéticaRESUMO
Embryonic development of the Asian tadpole shrimp Triops granarius is arrested at an early stage of organogenesis under darkness, whereas exposure to light resumes its development and promotes hatching. To better understand the photoreception systems involved in the light-induced hatching of T. granarius eggs, we examined the spectral sensitivity of hatching and the expression of genes involved in photoreception (opsins) and phototransduction (arrestin 2). Hatching of eggs is induced by light of broad wavelengths, from ultraviolet (UV) (395 nm) to red (660 nm), suggesting that visual pigments are involved in photoreception. Next, we focused on opsins which are protein moieties of visual pigments and arrestin 2 which is involved in a phototransduction cascade. Transcripts of several opsin genes and the arrestin 2 gene were detected in T. granarius eggs. Thus, we present a possibility that T. granarius eggs use visual pigments for light perception in light-induced hatching.
Assuntos
Crustáceos/genética , Crustáceos/efeitos da radiação , Transdução de Sinal Luminoso/genética , Transdução de Sinal Luminoso/efeitos da radiação , Luz , Óvulo/crescimento & desenvolvimento , Óvulo/efeitos da radiação , Sequência de Aminoácidos , Animais , Arrestinas/genética , Crustáceos/crescimento & desenvolvimento , Larva/genética , Larva/crescimento & desenvolvimento , Larva/metabolismo , Larva/efeitos da radiação , Dados de Sequência Molecular , Opsinas/genética , Óvulo/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Sensibilidade e Especificidade , Alinhamento de Sequência , beta-ArrestinasRESUMO
BACKGROUND: Technetium 99m-labeled radiopharmaceuticals accumulate in the liver and gallbladder, where they generate intensity artifacts that can result in misdiagnosis of myocardial single photon emission computed tomography (SPECT) images. This study identifies and eliminates factors affecting the magnitude and appearance of intensity artifacts in a gallbladder-heart phantom. METHODS AND RESULTS: The myocardium and background compartments of a phantom were filled with Tc-99m at concentrations of 320 and 26.1 kBq/mL, respectively. A disposable plastic syringe containing 5 mL of Tc-99m as a model of the gallbladder was fixed in a position lateral to the heart phantom. Artifact intensity was determined on SPECT images over a specific activity range in the syringe (28.6, 6.6, and 0.2 MBq/mL). Among 72 projection images, those with maximal heart counts in the region of interest were selected. Counts above and below 110% of the maximal heart count in all projection images were excluded and reconstructed, respectively. At 28.6 and 6.6 MBq/mL, excessive artifacts generated cold pixels immediately around the source, whereas lower activity (0.2 MBq/mL) caused the artifacts to disappear. Truncating the counts in the gallbladder caused the intensity artifacts at specific activities of 28.6 and 6.6 MBq/mL to disappear. CONCLUSIONS: The magnitude and appearance of intensity artifacts depend on contrast between extracardiac activities in the same slices of the heart in myocardial SPECT images with Tc-99m tetrofosmin, and pixel truncation can eliminate them.