RESUMO
OBJECTIVE: This study aimed to evaluate the effects of ingested periodontal pathogens on experimental colitis in mice and to elucidate its underlying mechanisms. BACKGROUND: Inflammatory bowel disease (IBD) is defined as a chronic intestinal inflammation that results in damage to the gastrointestinal tract. Epidemiological studies have shown an association between IBD and periodontitis. Although a large number of ingested oral bacteria reach gastrointestinal tract constantly, the effect of ingested periodontal pathogens on intestinal inflammation is still unknown. METHODS: Experimental colitis was induced by inclusion of dextran sodium sulfate solution in drinking water of the mice. Major periodontal pathogens (Porphyromonas gingivalis, Prevotella intermedia, and Fusobacterium nucleatum) were administered orally every day during the experiment. The severity of colitis between the groups was compared. In vitro studies of the intestinal epithelial cell line were conducted to explore the molecular mechanisms by which periodontal pathogens affect the development of colitis. RESULTS: The oral administration of P. gingivalis significantly increased the severity of colitis when compared to other pathogens in the DSS-induced colitis model. The ingested P. gingivalis disrupted the colonic epithelial barrier by decreasing the expression of tight junction proteins in vivo. In vitro permeability assays using the intestinal epithelial cell line suggested the P. gingivalis-specific epithelial barrier disruption. The possible involvement of gingipains in the exacerbation of colitis was implied by using P. gingivalis lacking gingipains. CONCLUSION: Porphyromonas gingivalis exacerbates gastrointestinal inflammation by directly interacting with the intestinal epithelial barrier in a susceptible host.
Assuntos
Colite , Porphyromonas gingivalis , Animais , Colite/induzido quimicamente , Colite/complicações , Ingestão de Alimentos , Fusobacterium nucleatum , Camundongos , Camundongos Endogâmicos C57BL , Prevotella intermediaRESUMO
Confirming mucosal healing is important in inflammatory bowel disease treatment. Complement C1q-mediated Wnt signaling activation has recently been suggested to mediate tissue repair and mucosal regeneration. We investigated the involvement of complement C1q and Wnt signaling in intestinal mucosal regeneration using a murine colitis model. The colitis model was established by providing C57BL/6J mice with 4% dextran sodium sulfate (DSS) for 1 week (inflammation phase) followed by regular water for 2 weeks (recovery phase). After 3 weeks, we investigated the relationship between C1q in serum and colonic tissue during the inflammation and recovery phases. We assessed Wnt signaling activity by evaluating ß-catenin expression in mouse intestinal tissue. Serum C1q levels were elevated during the recovery phase. C1q-specific staining indicated high C1q expression in pathological intestinal tissue during the inflammation and recovery phases. C1q mRNA and protein expression was increased during both phases. Interestingly, C1q-expressing cells were consistent with macrophages (F4/80-positive cells). Moreover, the expression of ß-catenin increased in the colonic tissues during the recovery phase of DSS-induced colitis but decreased during the inflammation phase of DSS-induced colitis. C1q expression may mediate Wnt signaling activity and intestinal epithelial regeneration.
Assuntos
Colite/metabolismo , Complemento C1q/genética , Mucosa Intestinal/fisiologia , Macrófagos/metabolismo , Regeneração , Via de Sinalização Wnt , Animais , Colite/genética , Colite/fisiopatologia , Modelos Animais de Doenças , Feminino , Regulação da Expressão Gênica , Inflamação , Macrófagos/imunologia , Camundongos , Camundongos Endogâmicos C57BL , Regulação para Cima , beta Catenina/genética , beta Catenina/metabolismoRESUMO
OBJECTIVES: To assess the relationship between the complement activation route and clinical manifestations in systemic lupus erythematosus (SLE). METHODS: Patients with SLE in whom complement activation occurred were divided into two groups: those in whom the complement system was mainly activated through the classical pathway (low serum C3 and C4 levels; CP group); and those in whom the complement system was solely activated through the alternative pathway (low serum C3 with normal C4 levels; AP group). Clinical manifestations were compared between the groups. RESULTS: The CP group had higher frequencies of arthritis, serositis, and nephritis, and a higher prevalence of anti-DNA antibodies compared to the AP group (arthritis: 50.0% vs. 13.0%, p = 0.0014; serositis: 37.5% vs. 13.0%, p = 0.0257; nephritis: 63.6% vs. 21.7%, p = 0.0003; anti-DNA antibodies: 73.9% vs. 30.4%, p = 0.0001). In contrast, the AP group had a higher frequency of anti-phospholipid (anti-PL) antibodies and a higher prevalence of antiphospholipid syndrome (APS) (anti-PL antibodies: 70.6% vs. 37.3%, p = 0.0136; APS: 39.1% vs. 5.7%, p < 0.0001). CONCLUSIONS: Our results suggest that a different complement system mechanism may act in the pathogenesis of APS in patients with SLE.
Assuntos
Ativação do Complemento/imunologia , Complemento C3 , Complemento C4 , Lúpus Eritematoso Sistêmico/diagnóstico , Adolescente , Adulto , Idoso , Anticorpos Antifosfolipídeos/sangue , Síndrome Antifosfolipídica/sangue , Síndrome Antifosfolipídica/diagnóstico , Síndrome Antifosfolipídica/imunologia , Feminino , Humanos , Lúpus Eritematoso Sistêmico/sangue , Lúpus Eritematoso Sistêmico/imunologia , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Adulto JovemRESUMO
The mechanisms by which commensal bacteria suppress inflammatory signalling in the gut are still unclear. Here, we present a cellular mechanism whereby the polarity of intestinal epithelial cells (IECs) has a major role in colonic homeostasis. TLR9 activation through apical and basolateral surface domains have distinct transcriptional responses, evident by NF-kappaB activation and cDNA microarray analysis. Whereas basolateral TLR9 signals IkappaBalpha degradation and activation of the NF-kappaB pathway, apical TLR9 stimulation invokes a unique response in which ubiquitinated IkappaB accumulates in the cytoplasm preventing NF-kappaB activation. Furthermore, apical TLR9 stimulation confers intracellular tolerance to subsequent TLR challenges. IECs in TLR9-deficient mice, when compared with wild-type and TLR2-deficient mice, display a lower NF-kappaB activation threshold and these mice are highly susceptible to experimental colitis. Our data provide a case for organ-specific innate immunity in which TLR expression in polarized IECs has uniquely evolved to maintain colonic homeostasis and regulate tolerance and inflammation.
Assuntos
Polaridade Celular , Colo/citologia , Enterócitos/citologia , Homeostase , Transdução de Sinais , Receptor Toll-Like 9/metabolismo , Animais , Células CACO-2 , Cloroquina/farmacologia , Colo/efeitos dos fármacos , Colo/patologia , Regulação da Expressão Gênica/efeitos dos fármacos , Homeostase/efeitos dos fármacos , Humanos , Tolerância Imunológica/efeitos dos fármacos , Ligantes , Camundongos , Camundongos Endogâmicos C57BL , Estrutura Terciária de Proteína , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Transdução de Sinais/efeitos dos fármacos , Receptor Toll-Like 9/químicaRESUMO
ABSTRACT: Inflammatory bowel disease (IBD) is caused by the activation of an abnormal immune response in the intestinal mucosa; the spleen is involved in the main immune response. Ulcerative colitis (UC) and Crohn disease (CD) have different inflammatory mechanisms; this study aimed to quantitatively measure and compare the spleen volumes between patients with UC and CD and examine the relationship between spleen volume and disease activity in both.We retrospectively analyzed 44 patients with IBD aged 30-60âyears (UC group, nâ=â24; CD group, nâ=â20). The control group comprised 19 patients with pancreatic cysts that did not affect the spleen volume. All patients underwent computed tomography (CT) between April 2014 and March 2019. Using the Image J software, spleen volumes in the UC, CD, and control groups were measured accurately from the CT images and adjusted for the body weight.No significant differences in the sex, age, or body weight were noted between the UC and CD groups and the control group. The spleen volumes, adjusted for the body weight, were 2.2â±â1.0âcm3/kg, 2.0â±â1.0âcm3/kg, and 3.6â±â1.7âcm3/kg in the control, UC, and CD groups, respectively. The volumes differed significantly between the CD and control groups (Pâ=â.01), but not between the UC and control groups (Pâ=â.43). Furthermore, a significant strong correlation was found between the disease activity and the body weight-adjusted spleen volume in patients with CD (Pâ<â.01).The spleen volume, adjusted for the body weight, was significantly larger in patients with CD than in the controls and was also strongly correlated with the CD activity. These results suggest that the immune response in CD may affect the spleen volume.
Assuntos
Colite Ulcerativa/diagnóstico por imagem , Doença de Crohn/diagnóstico por imagem , Baço/diagnóstico por imagem , Tomografia Computadorizada por Raios X/métodos , Adulto , Peso Corporal , Feminino , Humanos , Doenças Inflamatórias Intestinais , Masculino , Pessoa de Meia-Idade , Cisto Pancreático , Estudos RetrospectivosRESUMO
Anisakiasis involves the stomach in most cases and occurs rarely in the small intestine. Anisakiasis in the small intestine is associated with abdominal pain and obstruction and is rarely associated with intestinal bleeding. Unlike in the stomach, anisakiasis in the small intestine is difficult to diagnose anatomically. The patient in this case study developed hypovolemic shock due to excessive bleeding and underwent emergency surgery. With the recent increase in the consumption of raw fish around the world, this report provides an important finding of bleeding in the small intestine due to an unknown cause.
Assuntos
Anisaquíase/diagnóstico , Endoscopia , Intestino Delgado , Dor Abdominal/etiologia , Anisaquíase/patologia , Anisaquíase/cirurgia , Hemorragia Gastrointestinal/etiologia , Humanos , Obstrução Intestinal/etiologia , Masculino , Pessoa de Meia-Idade , Alimentos Crus , Alimentos MarinhosRESUMO
Experimental colitis is mediated by inflammatory or dysregulated immune responses to microbial factors of the gastrointestinal tract. In this study we observed that administration of Toll-like receptor 9 (TLR9) agonists suppressed the severity of experimental colitis in RAG1-/- but not in SCID mice. This differential responsiveness between phenotypically similar but genetically distinct animals was related to a partial blockade in TLR9 signaling and defective production of type I IFN (i.e., IFN-alpha/beta) in SCID mice upon TLR9 stimulation. The addition of neutralization antibodies against type I IFN abolished the antiinflammatory effects induced by TLR9 agonists, whereas the administration of recombinant IFN-beta mimicked the antiinflammatory effects induced by TLR9 agonists in this model. Furthermore, mice deficient in the IFN-alpha/beta receptor exhibited more severe colitis than wild-type mice did upon induction of experimental colitis. These results indicate that TLR9-triggered type I IFN has antiinflammatory functions in colitis. They also underscore the important protective role of type I IFN in intestinal homeostasis and suggest that strategies to modulate innate immunity may be of therapeutic value for the treatment of intestinal inflammatory conditions.
Assuntos
Colite/imunologia , Proteínas de Ligação a DNA/metabolismo , Interferon-alfa/imunologia , Interferon beta/imunologia , Receptores de Superfície Celular/metabolismo , Proteínas Adaptadoras de Transdução de Sinal , Animais , Antígenos de Diferenciação/genética , Antígenos de Diferenciação/metabolismo , Células Cultivadas , Colite/induzido quimicamente , Colo/citologia , Colo/imunologia , Colo/patologia , Meios de Cultivo Condicionados , Citocinas/imunologia , Proteína Quinase Ativada por DNA , Proteínas de Ligação a DNA/agonistas , Proteínas de Ligação a DNA/genética , Sulfato de Dextrana/administração & dosagem , Sulfato de Dextrana/toxicidade , Modelos Animais de Doenças , Proteínas de Homeodomínio/genética , Proteínas de Homeodomínio/metabolismo , Humanos , Indicadores e Reagentes/administração & dosagem , Indicadores e Reagentes/toxicidade , Proteínas de Membrana/genética , Proteínas de Membrana/metabolismo , Camundongos , Camundongos Endogâmicos , Camundongos Knockout , Camundongos SCID , Fator 88 de Diferenciação Mieloide , Proteínas Nucleares , Proteínas Serina-Treonina Quinases/genética , Proteínas Serina-Treonina Quinases/metabolismo , Receptor de Interferon alfa e beta , Receptores de Superfície Celular/agonistas , Receptores Imunológicos/genética , Receptores Imunológicos/metabolismo , Receptores de Interferon/genética , Receptores de Interferon/metabolismo , Receptor Toll-Like 9RESUMO
A 66-year-old man was diagnosed from colonoscopy as having a 40-mm elevated tumor in the cecum. With a preoperative diagnosis of intramucosal carcinoma, endoscopic submucosal dissection (ESD) was performed. The tumor was resected en bloc, yielding a specimen with a 66-mm diameter. No perforation was detected during the operation.Although neither abdominal pain nor fever was observed immediately after ESD, abdominal pain developed on the following day. Two days after ESD, the abdominal pain ceased. The patient was managed conservatively with fasting and intravenous antibiotic treatment. Four days after ESD, abdominal X-ray revealed marked gas retention. Computed tomography revealed pneumoperitoneum and a pelvic abscess, leading to a diagnosis of delayed perforation after colonic ESD and paralytic intestinal obstruction. A decompression tube was then inserted transnasally into the small intestine. Because a gradual decrease occurred in intestinal gas, the decompression tube was removed. Oral ingestion was resumed 13 days post-ESD.Delayed perforation after colonic ESD often requires emergency surgery. The present case was managed conservatively, despite paralytic intestinal obstruction. This approach is rarely employed for this condition and is therefore worth reporting.
Assuntos
Neoplasias do Colo/cirurgia , Ressecção Endoscópica de Mucosa/efeitos adversos , Perfuração Intestinal/etiologia , Idoso , Neoplasias do Ceco/diagnóstico , Neoplasias do Ceco/cirurgia , Neoplasias do Colo/diagnóstico , Tratamento Conservador , Humanos , Perfuração Intestinal/diagnóstico , Perfuração Intestinal/terapia , Masculino , Fatores de TempoRESUMO
Purpose: We aimed to discover glycosyltransferase gene (glycogene)-derived molecular subtypes of colorectal cancer associated with patient outcomes.Experimental Design: Transcriptomic and epigenomic datasets of nontumor, precancerous, cancerous tissues, and cell lines with somatic mutations, mismatch repair status, clinicopathologic and survival information were assembled (n = 4,223) and glycogene profiles were analyzed. IHC for a glycogene, GALNT6, was conducted in adenoma and carcinoma specimens (n = 403). The functional role and cell surface glycan profiles were further investigated by in vitro loss-of-function assays and lectin microarray analysis.Results: We initially developed and validated a 15-glycogene signature that can identify a poor-prognostic subtype, which closely related to deficient mismatch repair (dMMR) and GALNT6 downregulation. The association of decreased GALNT6 with dMMR was confirmed in multiple datasets of tumors and cell lines, and was further recapitulated by IHC, where approximately 15% tumors exhibited loss of GALNT6 protein. GALNT6 mRNA and protein was expressed in premalignant/preinvasive lesions but was subsequently downregulated in a subset of carcinomas, possibly through epigenetic silencing. Decreased GALNT6 was independently associated with poor prognosis in the IHC cohort and an additional microarray meta-cohort, by multivariate analyses, and its discriminative power of survival was particularly remarkable in stage III patients. GALNT6 silencing in SW480 cells promoted invasion, migration, chemoresistance, and increased cell surface expression of a cancer-associated truncated O-glycan, Tn-antigen.Conclusions: The 15-glycogene signature and the expression levels of GALNT6 mRNA and protein each serve as a novel prognostic biomarker, highlighting the role of dysregulated glycogenes in cancer-associated glycan synthesis and poor prognosis. Clin Cancer Res; 24(18); 4468-81. ©2018 AACR.
Assuntos
Neoplasias Colorretais/genética , N-Acetilgalactosaminiltransferases/genética , Polissacarídeos/genética , Prognóstico , Neoplasias Encefálicas/genética , Proliferação de Células/efeitos dos fármacos , Neoplasias Colorretais/classificação , Neoplasias Colorretais/tratamento farmacológico , Neoplasias Colorretais/patologia , Intervalo Livre de Doença , Feminino , Fluoruracila/farmacologia , Regulação Neoplásica da Expressão Gênica , Células HCT116 , Humanos , Masculino , Análise em Microsséries , Síndromes Neoplásicas Hereditárias/genética , Polissacarídeos/biossíntese , RNA Mensageiro/genética , Transcriptoma/genética , Polipeptídeo N-AcetilgalactosaminiltransferaseRESUMO
Depression is implicated as a risk factor for the recurrence of inflammatory bowel disease (IBD). Near-infrared spectroscopy (NIRS) and brain-derived neurotrophic factor (BDNF) are useful tools for evaluation of brain activity and a depressive state, respectively. The aim of this study was to clarify the association between brain activity or depressive symptoms and IBD using NIRS and BDNF. This study included 36 ulcerative colitis (UC) patients, 32 Crohn's disease (CD) patients, and 17 healthy controls (HC). Center for Epidemiologic Studies Depression Scale (CES-D) scores were determined, NIRS was performed, and serum BDNF levels were measured in all subjects. NIRS showed that the mean oxygenated hemoglobin concentration was significantly lower in the frontal lobe in the UC group than in the HC group (HC 167 ± 106 vs. UC 83.1 ± 85.3, p < 0.05). No significant difference was seen between the HC and CD groups. There were also no significant differences in CED-D scores and BDNF levels among the groups. Changes in the NIRS values of the UC group may indicate decreased brain activity and a fundamental difference between UC and CD, which are often lumped together as two types of IBD.
Assuntos
Encéfalo/diagnóstico por imagem , Colite Ulcerativa/psicologia , Doença de Crohn/psicologia , Depressão/diagnóstico por imagem , Espectroscopia de Luz Próxima ao Infravermelho/métodos , Adolescente , Adulto , Idoso , Fator Neurotrófico Derivado do Encéfalo/sangue , Estudos de Casos e Controles , Colite Ulcerativa/sangue , Colite Ulcerativa/diagnóstico por imagem , Doença de Crohn/sangue , Doença de Crohn/diagnóstico por imagem , Depressão/sangue , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Fatores de Risco , Adulto JovemRESUMO
Endoscopic ultrasonography (EUS) is considered the most sensitive imaging method for the diagnosis of chronic pancreatitis (CP). Several investigators have shown that EUS findings of CP correlate with the presence of CP on endoscopic retrograde pancreatography (ERP). In general, for diagnosing CP using EUS, the presence or absence of the following EUS criteria is determined: hyperechoic foci, hyperechoic strands, lobularity, shadowing calcifications, cysts, hyperechoic duct margins, visible side branches, main pancreatic duct dilatation, and main pancreatic duct irregularity. Using these criteria, we reviewed the number of EUS criteria required to diagnose early CP and whether each EUS criterion correlates with the severity of CP on ERP. CP is likely when more than three criteria (for "early CP") or more than five criteria (for "moderate CP") are present. Moreover, each EUS criterion has its own importance at each ERP classification level. However, the obtained images can be operator dependent, and interobserver variability may affect interpretation of CP by EUS. Therefore, we performed a quantitative computer analysis of parenchymal echogenicity and compared it with the EUS diagnosis of CP so that the diagnosis of CP on the basis of EUS criteria could be objectively supported by the quantitative analysis of EUS images. In conclusion, EUS can objectively distinguish between a normal pancreas and CP, and can be used to evaluate the severity of the CP. EUS is a useful modality for diagnosing CP and is relatively less invasive than other available modalities.
Assuntos
Endossonografia , Pancreatite Crônica/diagnóstico por imagem , Humanos , Processamento de Imagem Assistida por Computador , Reprodutibilidade dos Testes , Índice de Gravidade de DoençaRESUMO
AIMS: To investigate whether Imiquimod (IMQ) as TLR7 ligand protects mice from colonic inflammation and the mechanisms underlying in such immunoregulatory conditions.ãMETHODS: Murine colitis was induced to Balb/c mice by administration of trinitrobenzene sulfonic acid (TNBS) with or without daily intraperitoneal administration of IMQ.ãColitis was evaluated by body weight decreases and by histological score.ãAlso colonic mRNA expression was measured by RT-PCR.ãTo confirm the induction of Regulatory T cells (Tregs) by type-1 IFN from pDCs, we generated mouse bone marrow-derived pDCs and co-cultured these with CD4+ T cells isolated from mouse spleen with or without IMQ stimulation.ãCytokine production in the culture supernatant was measured by ELISA and the number of Tregs were analyzed by flow cytometry.ãSpleen and mesenteric lymph nodes (MLN) from IMQ-treated mice were collected, and mRNA expressions of cytokine were measured by RT-PCR and cytokine productions were measured by ELISA.ãTregs and chemokine expressions were analyzed in colon of TNBS-induced colitis mouse by immunohistochemistry.ãRESULTS: Administration of IMQ significantly suppressed colonic inflammation of TNBS-induced colitis.ãIn the colons of IMQ-treated mice, mRNA expression of TNF-α was decreased, and strong expressions of IL-6, IFN-ß and TGF-ß were detected.ãIL-10 and TGF-ß productions were increased in the supernatant of co-cultured cells stimulated with IMQ, although we were unable to detect Treg differentiaton in IMQ-stimulated co-cultured cells.ãIn MLN of IMQ-treated mice, strong expressions of TLR7, IFN-ß, TGF-ß and Foxp3 mRNA were detected.ãIL-10 production from MLN cells was also increased in the IMQ-treated group.ãFinally, Tregs in the inflamed colon and CCR9 in MLN of IMQ-treated mice were detected.ãCONCLUSION: These results suggest that IMQ protects mice from TNBS colitis through induction of CCR9, which regulates accumulation of Tregs in the inflamed colon.
Assuntos
Aminoquinolinas/farmacologia , Colite/tratamento farmacológico , Receptores CCR/fisiologia , Aminoquinolinas/uso terapêutico , Animais , Diferenciação Celular/efeitos dos fármacos , Células Cultivadas , Colite/induzido quimicamente , Feminino , Imiquimode , Glicoproteínas de Membrana/fisiologia , Camundongos , Camundongos Endogâmicos BALB C , Linfócitos T Reguladores/efeitos dos fármacos , Receptor 7 Toll-Like/fisiologia , Ácido TrinitrobenzenossulfônicoRESUMO
An 83-year-old female began treatment with prednisolone and ursodeoxycholic acid at 62 years of age, following a diagnosis of primary biliary cirrhosis (PBC) and secondary Sjögren's syndrome (SjS). With persisting bloody stools, the patient underwent colonoscopy at 83 years of age. Histopathological evaluation revealed mucosa-associated lymphoid tissue (MALT) lymphoma. The elevated rectal lesion resolved with rituximab treatment. We report this case because although patients with SjS are at increased risk of malignant lymphoma, primary rectal MALT lymphoma is very uncommon in association with PBC and secondary SjS.
Assuntos
Cirrose Hepática Biliar/complicações , Linfoma de Zona Marginal Tipo Células B/etiologia , Neoplasias Retais/etiologia , Síndrome de Sjogren/complicações , Antineoplásicos/uso terapêutico , Biópsia , Colo/patologia , Colonoscopia , Feminino , Humanos , Linfoma de Zona Marginal Tipo Células B/diagnóstico , Linfoma de Zona Marginal Tipo Células B/tratamento farmacológico , Linfoma de Zona Marginal Tipo Células B/patologia , Pessoa de Meia-Idade , Tomografia por Emissão de Pósitrons combinada à Tomografia Computadorizada , Neoplasias Retais/diagnóstico , Neoplasias Retais/tratamento farmacológico , Neoplasias Retais/patologia , Rituximab/uso terapêuticoRESUMO
Stressful life situation can trigger the onset and flare-ups of Behçet's disease (BD). In addition, the association of systemic sclerosis (SSc) and BD is rare. In this study, we report a patient who had Sjögren's syndrome as a primary disease and subsequently developed SSc and BD after an earthquake disaster and the death of her father.
Assuntos
Síndrome de Behçet/etiologia , Desastres , Terremotos , Escleroderma Sistêmico/etiologia , Adulto , Feminino , HumanosRESUMO
We report a case of rectal varices that developed after endoscopic injection sclerotherapy (EIS) and Hassab's operation for esophageal varices with extrahepatic portal obstruction. A 54-year-old woman was admitted to our hospital in September 1997 for treatment of hematochezia. Emergent colonoscopy revealed tortuous rectal varices with a white plug. Angiography revealed that rectal varices were provided with backward blood flow by the inferior mesenteric vein due to extrahepatic portal obstruction. In this case, previous treatment, EIS and Hassab's operation, for esophago-gastric varices might have inhibited the development of collaterals apart from surface of gastrointestinal tract, such as para-esophageal collateral veins or spleno-renal shunt. Since the thrombus in the extrahepatic portal vein causes strong pressure on inferior mesenteric vein which is connected to the inferior vena cava via the inferior rectal vein, rectal varices might be developed. In this case, it was considered that rectal varices were not treated enough by endoscopic therapy because of regurgitant hyper blood flow against portal venous pressure. Therefore, rectal transection was performed. After the treatment, the patient suffered no further episodes of bleeding from rectal varices.
Assuntos
Varizes Esofágicas e Gástricas/terapia , Doenças Retais/etiologia , Doenças Vasculares/etiologia , Varizes Esofágicas e Gástricas/etiologia , Feminino , Humanos , Hipertensão Portal/complicações , Pessoa de Meia-Idade , Veia Porta/patologia , EscleroterapiaRESUMO
BACKGROUND: Neutrophil elastase (NE) is a proteinase in granulocytes and plays an important role in the pathogenesis of inflammatory disorders. It has been reported that NE activity is elevated in both colonic mucosa and blood in inflammatory bowel disease (IBD) patients, and that it can act as an aggravating factor in IBD. To develop novel therapies for IBD, we examined the effects of an NE inhibitor, Elaspor®, on murine experimental colitis. METHODS: Acute colitis was induced in BALB/c mice by administration of dextran sulfate sodium (DSS) in drinking water for 7 days. NE inhibitor was administered subcutaneously to mice prior to and during the induction of colitis. Disease activity index (DAI), colonic myeloperoxidase (MPO) activity, luminal NE activity, and mRNA expression in the colon were then investigated. RESULTS: Subcutaneous administration of NE inhibitor ameliorated the severity of DSS-induced colitis. NE activity was elevated in inflamed colon, and was reduced by NE inhibitor administration. mRNA expression levels of IL-17, a Th17-based inflammatory factor, was also decreased in the colon of NE inhibitor-administered mice. CONCLUSION: These results suggest that NE inhibitor ameliorated colonic inflammation by decreasing both the activity of NE and the effects of cytokine balance. Clinically, NE inhibitor improves injuries associated with systemic inflammatory response syndrome. Similarly, clinical use of this inhibitor would further clarify its usefulness in clinical colonic inflammation.
Assuntos
Colite/prevenção & controle , Interleucina-17/metabolismo , Proteínas Secretadas Inibidoras de Proteinases/farmacologia , Animais , Quimiocinas/genética , Colite/etiologia , Colite/metabolismo , Colo/efeitos dos fármacos , Colo/metabolismo , Colo/patologia , Citocinas/genética , Sulfato de Dextrana/toxicidade , Modelos Animais de Doenças , Feminino , Glicina/análogos & derivados , Glicina/farmacologia , Interleucina-17/genética , Elastase de Leucócito/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , Peroxidase/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Sulfonamidas/farmacologiaRESUMO
A 39-year-old man presented with diarrhea and abdominal pain. At 26 years of age, he was found to have eosinophilia and abnormal liver function parameters, for which prednisolone therapy was started. He subsequently underwent a liver biopsy and endoscopic retrograde cholangiopancreatography, and received a diagnosis of primary sclerosing cholangitis (PSC). On presentation to our hospital, he was further diagnosed with eosinophilic colitis based on aggravation of diarrhea and severe eosinophilic infiltration in the colonic mucosa. We herein report a rare case of concurrent PSC and eosinophilic colitis.
Assuntos
Colangite Esclerosante/complicações , Colite/complicações , Eosinofilia/complicações , Dor Abdominal/etiologia , Adulto , Anti-Inflamatórios/uso terapêutico , Biópsia , Colangiopancreatografia Retrógrada Endoscópica , Colangite Esclerosante/diagnóstico , Colite/diagnóstico , Diagnóstico Diferencial , Diarreia/etiologia , Eosinofilia/diagnóstico , Hepatite Autoimune/diagnóstico , Hepatite Autoimune/tratamento farmacológico , Humanos , Fígado/patologia , Masculino , Prednisolona/uso terapêuticoRESUMO
Inflammatory bowel disease (IBD) is a chronic inflammatory disorder caused by dysregulated immune responses in a genetically predisposed individual. Recent accumulating data, including genome-wide association studies, have identified >100 distinct genetic loci that confer susceptibility. We highlight how the dysregulation of host-microbial interaction leads to intestinal inflammation, particularly with respect to the overlap of common genetic and pathophysiological mechanisms of IBD.