RESUMO
The catfish subfamily Trichomycterinae is widely distributed in South America inhabiting several habitats, but specially mountain streams. Trichomycterus is the most speciose trichomycterid genus and recently due to his paraphyletic condition has been restricted to a clade from eastern Brazil called Trichomycterus sensu stricto comprising around 80 valid species distributed in seven areas of endemism of eastern Brazil. This paper aims to analyse the biogeographical events responsible for the distribution of Trichomycterus s.s., by reconstructing the ancestral data based on a time-calibrated multigene phylogeny. A multi-gene phylogeny was generated using 61 species of Trichomycterus s.s. and 30 outgroups, with divergence events calculated based on the estimated origin of Trichomycteridae. Two event-based analyses were applied to investigate the biogeographical events responsible the present distribution of Trichomycterus s.s. and suggest that the modern distribution of the group is a result of different vicariance and dispersal events. The diversification of Trichomycterus s.s. subgenera occurred in the Miocene, except for Megacambeva, with different biogeographical events shaping its distribution in eastern Brazil. An initial vicariant event split up the Fluminense ecoregion from the Northeastern Mata Atlantica + Paraíba do Sul + Fluminense + Ribeira do Iguape + Upper Paraná ecoregions. Dispersal events occurred mainly between Paraíba do Sul and neighboring river basins, with additional dispersal events from Northeastern Mata Atlantica to Paraíba do Sul, from São Francisco to Northeastern Mata Atlântica, and from Upper Paraná to São Francisco.
Assuntos
Peixes-Gato , Animais , Filogenia , Brasil , Peixes-Gato/genética , RiosRESUMO
The relationship between the (Na(+) and K(+))-activated adenosine triphosphatase enzyme system implicated in sodium-transport by cell membranes and the calcium-activated adenosine triphosphatase, which is generally associated with calcium uptake, was examined in microsomes from skeletal muscle. Whereas sodium and potassium did not modify the relatively low adenosine triphosphatase activity seen in the absence of calcium, a pattern similar to that of the sodium-transport enzyme system was seen afer the addition of CaCl(2). The calcium-activated adenosine triphosphatase was stimulated equally by sodium or potassium alone, but both the rate and extent of calcium uptake were enhanced more by potassium than by sodium at concentrations below 0.12 mole per liter. In the absence of either of these ions addition of calcium failed to activate adenosine triphosphatase although significant amounts of calcium were taken up by the microsomes.
Assuntos
Adenosina Trifosfatases/metabolismo , Cálcio/metabolismo , Microssomos/enzimologia , Músculos/efeitos dos fármacos , Potássio/farmacologia , Sódio/farmacologia , Animais , Transporte Biológico Ativo , Cloreto de Cálcio/farmacologia , Microssomos/efeitos dos fármacos , Músculos/citologia , Músculos/enzimologia , Músculos/metabolismo , CoelhosRESUMO
The control by calcium ion of the adenosine triphosphatase activity of cardiac actomyosin is similar to that of white skeletal actomyosin. This finding indicates that the slower contraction and relaxation of heart muscle do not reflect different levels to which free calcium ion concentration around the myofibrils must be adjusted during contraction and relaxation and suggests a mechanism whereby myocardial contractility may be regulated.
Assuntos
Cálcio/farmacologia , Coração/efeitos dos fármacos , Contração Muscular/fisiologia , Proteínas Musculares , Miocárdio/enzimologia , Adenosina Trifosfatases , Animais , Cães , Técnicas In Vitro , CoelhosRESUMO
Circulation Research, first published in 1953, was created by the American Heart Association as "the authoritative new journal for investigators of the basic sciences as they apply to the heart and circulation." This review of the early years of the journal highlights the contributions of the first four Editors: Carl J. Wiggers, Carl F. Schmidt, Eugene M. Landis, and Julius H. Comroe, Jr. The success of Circulation Research is seen not only in the high quality of the articles published in its pages but also in the remarkable improvements in prevention and treatment of cardiovascular disease that have occurred over the past half century.
Assuntos
Circulação Sanguínea , Sistema Cardiovascular , Publicações Periódicas como Assunto/história , American Heart Association/história , História do Século XX , História do Século XXI , Filosofia Médica/história , Pesquisa/história , Estados UnidosRESUMO
The peptide compositions of rabbit skeletal- and canine cardiac-muscle sarcoplasmic reticulum preparations have been compared by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate. The cardiac preparations contain many proteins in addition to the 105 000 dalton peptide which has been previously identified as the Ca2+ stimulated ATPase. Four peptide components iodinated in the presence of either free or Sepharose 4B-bound lactoperoxidase have molecular weights of 130 000 (component I), 105 000 (component II), 52 000 (component III) and 47 000 (component IV). Comparison of the labelling patterns in the presence of the detergent Triton X-100 suggests that components I, III and IV have part of their peptide internally located. Although part of component II is externally accessible to free lactoperoxidase, its iodination is decreased by Triton X-100. Iodination of phospholamban, the 22 000 dalton substrate for cyclic AMP-dependent protein kinase, was not observed under the conditions investigated.
Assuntos
Radioisótopos do Iodo , Lactoperoxidase/metabolismo , Proteínas de Membrana/metabolismo , Proteínas Musculares/metabolismo , Miocárdio/metabolismo , Peroxidases/metabolismo , Retículo Sarcoplasmático/metabolismo , Animais , Cães , Eletroforese Descontínua , Peso Molecular , Miocárdio/ultraestrutura , Fosfoproteínas/metabolismo , CoelhosRESUMO
Calcium release from sarcoplasmic reticulum vesicles presumably derived from longitudinal tubules (LSR) and terminal cisternae (HSR) of rabbit skeletal muscle was investigated by dual wavelength spectrophotometry using the calcium-indicator antipyrylazo III. In 120 mM KCl, 5 mM MgCl2, 30 microM, CaCl2, 50 microM MgATP, 100 microM antipyrylazo III, 40 mM histidine (pH 6.8, 25 degrees C), LSR and HSR sequestered approx. 115 nmol calcium/mg, and then spontaneously released calcium. Analysis of ATP hydrolysis and phosphoenzyme level during LSR and HSR calcium sequestration indicated that this calcium release process was passive, occurring in the virtual absence of ATP and phosphoenzyme. Moreover, subsequent addition of ATP reinitiated the calcium sequestration-release sequence. Calcium release by HSR was more than 4-times faster than that by LSR. Analysis of the calcium release phase demonstrated a biexponential decay for both LSR (0.10 and 0.63 min-1) and HSR (0.26 and 1.65 min-1), suggestive of heterogeneity within each fraction. Replacement of 120 mM KCl with either 120 mM choline chloride, 240 mM sucrose, or H2O reduced maximal calcium sequestration by LSR, but had less effect on LSR calcium release rate constants. In the case of HSR, these changes in the ionic composition of the medium drastically reduced calcium release rate constants with little effect on calcium content. These marked differences between LSR and HSR are consistent with the hypothesis that the calcium permeability of the terminal cisternae is greater and more sensitive to the ionic environment than is that of the longitudinal tubules of sarcoplasmic reticulum.
Assuntos
Cálcio/metabolismo , Retículo Sarcoplasmático/metabolismo , Trifosfato de Adenosina/metabolismo , Animais , Gramicidina/farmacologia , Peso Molecular , Concentração Osmolar , Coelhos , Retículo Sarcoplasmático/efeitos dos fármacos , Espectrofotometria , Valinomicina/farmacologiaRESUMO
The effects of dimethylsulfoxide, propranolol and chlorpromazine on the partial reactions of the ATPase of sarcoplasmic reticulum were investigated. When analyzed according to a reaction scheme in which the ADP-sensitive (E1P) and ADP-insensitive (E2P) phosphoenzymes occur sequentially and P1 is derived from the latter, dimethylsulfoxide decreased the rate of E2P hydrolysis whereas it stimulated the rate of the E1P to E2P conversion. Propranolol increased the rate of E2P hydrolysis while it decreased the rate of the E1P to E2P conversion. Propranolol exerted an additional effect, presumably inhibition of the phosphoenzyme formation. These effects of dimethylsulfoxide and propranolol can account for both the stimulatory and inhibitory effects of these drugs on the overall rate of ATP hydrolysis observed in the presence and absence of added alkali metal salts. Chlorpromazine accelerated E2P hydrolysis whereas it appeared to inhibit the E1P to E2P conversion. These effects of chlorpromazine appear able to account for its stimulatory and inhibitory effects on the overall rate of ATP hydrolysis in the presence and absence of alkali metal salts. In the presence of chlorpromazine, however, the rate of Pi liberation during the steady state ATP hydrolysis was found to be greater than the hydrolysis rate of E2P. This finding suggests that under these conditions Pi is derived not only from E2P but also from source(s) other than E2P.
Assuntos
Adenosina Trifosfatases/metabolismo , Clorpromazina/farmacologia , Dimetil Sulfóxido/farmacologia , Propranolol/farmacologia , Retículo Sarcoplasmático/enzimologia , Difosfato de Adenosina/farmacologia , Animais , Ativação Enzimática , Cinética , Magnésio/farmacologia , Músculos/enzimologia , Cloreto de Potássio/farmacologia , CoelhosRESUMO
The mechanism of doxorubicin-induced Ca2+ release from skeletal and cardiac muscle sarcoplasmic reticulum (SR) was studied by examining the effects of azumolene (a water soluble dantrolene analog) on doxorubicin-mediated Ca2+ release and ryanodine binding. Doxorubicin induced a rapid Ca2+ release from both skeletal and cardiac SR in a similar concentration range (EC50 = 5-10 microM). Maximal doxorubicin-induced Ca2+ release was seen at 2 and 0.2 microM Ca2+ for skeletal and cardiac SR, respectively. Addition of 400 microM azumolene caused approx. 30% inhibition of doxorubicin-induced Ca2+ release from both skeletal and cardiac SR; skeletal SR had significantly higher sensitivity to azumolene than cardiac SR. In the presence of Ca2+, doxorubicin increased [3H]ryanodine binding to both skeletal and cardiac SR; whereas in the absence of Ca2+, doxorubicin led to significant ryanodine binding to skeletal SR, but not to cardiac SR. In both types of SR, doxorubicin-activated, but not Ca2+ activated ryanodine binding was inhibited by azumolene. Azumolene sensitivity for inhibition of doxorubicin-activated ryanodine binding was much higher in skeletal SR than cardiac SR, consistent with the results for effects of azumolene on Ca2+ release. Our results are consistent with the possibility that azumolene inhibits doxorubicin binding by direct competition for the drug receptor(s).
Assuntos
Cálcio/metabolismo , Doxorrubicina/farmacologia , Imidazóis/farmacologia , Músculos/metabolismo , Miocárdio/metabolismo , Oxazóis/farmacologia , Retículo Sarcoplasmático/metabolismo , Animais , Ligação Competitiva , Coelhos , Receptores Colinérgicos/efeitos dos fármacos , Rianodina/metabolismo , Canal de Liberação de Cálcio do Receptor de RianodinaRESUMO
The calcium activation of the ATPase (ATP phosphohydrolase, EC 3.6.1.3) activity of cardiac actomyosin reconstituted from bovine cardiac myosin and a complex of actin-tropomyosin-troponin extracted from bovine cardiac muscle at 37 degrees C was studied and compared with similar proteins from rabbit fast skeletal muscle. The proteins of the actin complex were identified by polyacrylamide gel electrophoresis in sodium dodecyl sulfate. Half-maximal activation of the cardiac actomyosin was seen at a calcium concentration of 1.2 +/- 0.002 (S.E. of mean) muM. A hybridized reconstituted actomyosin made with cardiac myosin and the actin-tropomyosin-troponin complex extracted from rabbit skeletal muscle was also activated by calcium but the half-maximal value was shifted to 0.65 +/- 0.02 (S.E. of mean) muM Ca2+. Homologous rabbit skeletal actomyosin showed half-maximal activation at 0.90 +/- 0.01 (S.E. of mean) muM Ca2+ and the value for a hybridized actomyosin made with rabbit skeletal myosin and the actin-complex from cardiac muscle was found at 1.4 +/- 0.03 (S.E. of mean) muM Ca2+ concentration. Kinetic analysis of the Ca2+ activated ATPase activity of reconstituted bovine cardiac actomyosin indicated some degree of cooperativity with respect to calcium. Double reciprocal plots of reconstituted actomyosins made with bovine cardiac actin complex were curvilinear and significantly different than those of reconstituted actomyosins made with the rabbit fast skeletal actin complex. The Ca2+-dependent cooperativity was of a mixed type as determined from Hill plots for homologous reconstituted bovine cardiac and rabbit fast skeletal actomyosin. The results show that cooperative interactions in reconstituted actomyosins were greater when the actin-tropomyosin-troponin complex was derived from cardiac than skeletal muscle.
Assuntos
Proteínas Musculares/metabolismo , Músculos/metabolismo , Miocárdio/metabolismo , Actinas/metabolismo , Actomiosina/metabolismo , Adenosina Trifosfatases/metabolismo , Trifosfato de Adenosina/metabolismo , Animais , Cálcio/farmacologia , Bovinos , Miosinas/metabolismo , Coelhos , Tropomiosina/metabolismo , Troponina/metabolismoRESUMO
The effects of adenylyl methylene diphosphate (AMD), a non-hydrolyzable ATP analogue, were examined in sarcoplasmic reticulum vesicles isolated from rabbit skeletal muscle. The Ca2+-dependent APTase activity measured at 5 degrees C and pH 7.0 in 5.2 micrometer [gamma-32P]ATP and in the absence of added alkali metal salts was stimulated by added AMD. The steady state level of phosphoenzyme, however, was not decreased greatly by added AMP under these conditions. The hydrolysis of the phosphoenzyme formed at the steady state in the absence of added alkali metal salts was accelerated by added AMD to an extent that can account for the stimulation of the ATPase activity. At 5 degrees C and pH 7.0 the maximum stimulation of phosphoenzyme hydrolysis by AMD and the Km value for this ATP analogue were 4.3-fold and 40 micrometer, respectively. These results provide further support for our previous conclusion (Shigekawa, M., Dougherty, J.P. and Katz, A.M. (1978) J.Biol. Chem. 253, 1442--1450) that 2 classes of ATP site exist in the calcium pump ATPase in the absence of added alkali metal salts, one being the catalytic site and the other being the regulation site which activates the activity of the catalytic site.
Assuntos
Difosfato de Adenosina/análogos & derivados , ATPases Transportadoras de Cálcio/metabolismo , Retículo Sarcoplasmático/enzimologia , Difosfato de Adenosina/farmacologia , Trifosfato de Adenosina/metabolismo , Animais , Sítios de Ligação , Ativação Enzimática/efeitos dos fármacos , Técnicas In Vitro , CoelhosRESUMO
Calcium uptake by rabbit skeletal muscle sarcoplasmic reticulum vesicles in phosphate-containing media exhibits time-dependent changes that arise from changing rates of calcium influx and efflux. The monovalent cation ionophore gramicidin, added before the start of the calcium uptake reaction, delayed the spontaneous calcium release that normally occurred after approx. 6 min in such reactions; the rate of calcium efflux was inhibited while calcium influx was little affected. Under these conditions, Ca2+-activated ATPase activity could remain unaltered. Gramicidin stimulated calcium uptake irrespective of the presence of a K+ gradient across the vesicle membrane. Valinomycin stimulated calcium uptake in a manner similar to that for gramicidin even in an NaCl-containing medium lacking potassium. Thus, dissipation of a transmembrane K+ gradient is unlikely to account for the effects of these ionophores on the spontaneous changes in calcium flux rates. Addition of gramicidin to partially calcium-filled vesicles inhibited the phase of spontaneous calcium reuptake because both calcium influx and efflux wre inhibited. Addition of gramicidin to partially calcium-filled vesicles in the presence of a water-soluble protein, such as bovine serum albumin, creatine kinase or pyruvate kinase, markedly stimulated calcium uptake. This stimulatory effect was due primarily to inhibition of calcium efflux, calcium influx being minimally influenced by the ionophore. After cleavage of the 100,000 dalton ATPase to 50,000 dalton fragments, which was not associated with changes in Ca2+-activated ATPase activity or initial calcium uptake rate, gramicidin increased rather than decreased calcium content when added to vesicles after the initial maximum in calcium content. Thus, the ability of monovalent cation ionophores to block calcium efflux from calcium-filled vesicles may reflect their interaction with a portion of the Ca2+-activated ATPase protein.
Assuntos
Cálcio/metabolismo , Gramicidina/farmacologia , Retículo Sarcoplasmático/metabolismo , Valinomicina/farmacologia , Animais , Transporte Biológico Ativo/efeitos dos fármacos , ATPases Transportadoras de Cálcio/metabolismo , Cinética , Músculos/metabolismo , Coelhos , Retículo Sarcoplasmático/efeitos dos fármacosRESUMO
Low concentrations of fatty acids inhibited initial Ca uptake by sarcoplasmic reticulum vesicles, the extent of inhibition varying with chain length and unsaturation in a series of C14-C20 fatty acids. Oleic acid was a more potent inhibitor of initial Ca uptake than stearic acid at 25 degrees C, whereas at 5 degrees C there was less difference between the inhibitory effects of low concentrations of these fatty acids. When the fatty acids were added later, during the phase of spontaneous Ca release that follow Ca uptake in reactions carried out at 25 degrees C 1-4 microM oleic and stearic acids caused Ca content to increase. This effect was due to marked inhibition of Ca efflux and slight stimulation of Ca influx. At concentrations of greater than 4 microM, both fatty acids inhibited the Ca influx that occurs during spontaneous Ca release; in the case of oleic acid, this inhibition resembled that of initial Ca uptake at 5 degrees C. The different effects of fatty acids at various times during Ca uptake reactions may be explained in part if alterations in the physical state of the membranes occur during the transition from the phase of initial Ca uptake to that of spontaneous Ca release.
Assuntos
Cálcio/metabolismo , Ácidos Graxos não Esterificados/farmacologia , Músculos/metabolismo , Retículo Sarcoplasmático/metabolismo , Animais , Transporte Biológico Ativo/efeitos dos fármacos , Cinética , Ácido Oleico , Ácidos Oleicos/farmacologia , Coelhos , Retículo Sarcoplasmático/efeitos dos fármacos , Ácidos Esteáricos/farmacologia , Relação Estrutura-AtividadeRESUMO
Unfractionated and low buoyant density sarcoplasmic reticulum vesicles released calcium spontaneously after ATP- or acetyl phosphate-supported calcium uptake when internal Ca2+ was stabilized by the use of 50 mM phosphate as calcium-precipitating anion. This spontaneous calcium release could not be attributed to falling Ca2+ concentration outside the vesicles (Ca02+), substrate depletion, ADP accumulation, nonspecific membrane deterioration of the attainment of a high vesicular calcium content. Instead, spontaneous calcium release was directly proportional to Ca02+ at the time that calcium content was maximal. A causal relationship between high Ca02+ and spontaneous calcium release was suggested by the finding that elevation of Ca02+ from less than 1 microM to 3--5 microM increased the rate and extent of calcium release. The spontaneous calcium release was due both to acceleration of calcium efflux and slowing of calcium influx that was not accompanied by a significant change in the rate of ATP hydrolysis. Neither reversal of the transmembrane KCl gradient nor incubation with cation and proton ionophores abolished the spontaneous calcium release. The persistence of calcium release under conditions where the membrane was permeable to both anions and cations makes it unlikely that this phenomenon is due to a changing transmembrane potential. The similarity between the Ca2+ dependence of spontaneous calcium release and of calcium uptake, along with other similarities between these processes, suggest that calcium release is mediated by the calcium pump in these membranes.
Assuntos
Cálcio/metabolismo , Músculos/metabolismo , Retículo Sarcoplasmático/metabolismo , Acetatos/farmacologia , Trifosfato de Adenosina/metabolismo , Trifosfato de Adenosina/farmacologia , Animais , Cloreto de Cálcio/farmacologia , Cátions Bivalentes , Hidrólise , Ionóforos/farmacologia , Organofosfatos , Compostos Organofosforados/farmacologia , Cloreto de Potássio/farmacologia , Coelhos , Fatores de TempoRESUMO
The heart's physiological performance, unlike that of skeletal muscle, is regulated primarily by variations in the contractile force developed by the individual myocardial fibers. In an attempt to identify the basis for the characteristic properties of myocardial contraction, the individual cardiac contractile proteins and their behavior in contractile models in vitro have been examined. The low shortening velocity of heart muscle appears to reflect the weak ATPase activity of cardiac myosin, but this enzymatic activity probably does not determine active state intensity. Quantification of the effects of Ca(++) upon cardiac actomyosin supports the view that myocardial contractility can be modified by changes in the amount of calcium released during excitation-contraction coupling. Exchange of intracellular K(+) with Na(+) derived from the extracellular space also could enhance myocardial contractility directly, as highly purified cardiac actomyosin is stimulated when K(+) is replaced by an equimolar amount of Na(+). On the other hand, cardiac glycosides and catecholamines, agents which greatly increase the contractility of the intact heart, were found to be without significant actions upon highly purified reconstituted cardiac actomyosin.
Assuntos
Coração/fisiologia , Proteínas Musculares/metabolismo , Adenosina Trifosfatases/metabolismo , Álcalis/farmacologia , Transporte Biológico , Glicosídeos Cardíacos/farmacologia , Catecolaminas/farmacologia , Espaço Extracelular/metabolismo , Magnésio/farmacologia , Contração Muscular , Potássio/metabolismo , Sódio/metabolismoRESUMO
Each cardiac cycle can be characterized by a pressure-volume loop that graphically depicts the external work of the ventricle. The pressure-volume loop is determined to a large extent by end-diastolic and end-systolic conditions, each of which reflects the properties of the heart and circulation at these two critical times in the cardiac cycle. The pressure and volume at end-diastole and end-systole, in turn, are determined by interactions between the heart and the circulation. End-diastolic pressure and volume reflect preload (venous return) and the lusitropic (relaxation) state of the ventricular walls, and end-systolic pressure and volume are determined by the afterload (peripheral and pulmonary resistance) and the inotropic (contractile) state of the myocardium. Alterations in inotropic and lusitropic state lead to predictable changes in the pressure-volume loop that, when combined with knowledge of preload and afterload, can facilitate understanding of the pharmacologic and pathophysiologic responses of the heart.
Assuntos
Coração/fisiologia , Contração Miocárdica , Volume Sistólico , Pressão Sanguínea , Coração/fisiopatologia , Frequência Cardíaca , Humanos , Função VentricularRESUMO
Forty years ago therapy for congestive heart failure was limited largely to the mercurial diuretics and a variety of cardiac glycoside preparations; these were often ineffective, and the common practice of "pushing" digitalis caused serious, sometimes lethal side effects. Today, a more complete understanding of the regulation of cardiac work and pathophysiology of heart failure is having a profound impact on therapeutic strategy for this common condition. Despite more powerful means to augment myocardial contractility and much more effective diuretics, therapy that relies only on inotropic stimulation and diuresis is no longer optimal for the majority of patients with heart failure. Thus, strategies for the therapy of heart failure must take into account new understanding of mechanisms that initiate, perpetuate and exacerbate the hemodynamic and myocardial abnormalities in these patients. Recognition of the detrimental effects of excessive afterload and the importance of relaxation (lusitropic) as well as contraction (inotropic) abnormalities has led to widespread acceptance of vasodilator therapy, which has dramatically improved our ability to alleviate the symptoms of heart failure. Changes that result from altered gene expression in the hypertrophied myocardium of patients with congestive heart failure can give rise to a cardiomyopathy of overload that, although initially compensatory, may hasten death. These and other advances in our understanding of the pathophysiology, biochemistry and molecular biology of heart failure provide a basis for new therapeutic strategies that can slow the progressive myocardial damage that causes many of these patients to die, while at the same time improving well-being in patients with congestive heart failure.
Assuntos
Insuficiência Cardíaca/terapia , Cardiotônicos/uso terapêutico , Glicosídeos Digitálicos/uso terapêutico , Diuréticos/uso terapêutico , Insuficiência Cardíaca/fisiopatologia , Humanos , Vasodilatadores/uso terapêuticoRESUMO
The flux of calcium ions (Ca2+) into the cytosol, where they serve as intracellular messengers, is regulated by two distinct families of Ca2+ channel proteins. These are the intracellular Ca2+ release channels, which allow Ca2+ to enter the cytosol from intracellular stores, and the plasma membrane Ca2+ channels, which control Ca2+ entry from the extracellular space. Each of these two families of channel proteins contains several subgroups. The intracellular channels include the large Ca2+ channels ("ryanodine receptors") that participate in cardiac and skeletal muscle excitation-contraction coupling, and smaller inositol trisphosphate (InsP3)-activated Ca2+ channels. The latter serve several functions, including the pharmacomechanical coupling that activates smooth muscle contraction, and possibly regulation of diastolic tone in the heart. The InsP3-activated Ca2+ channels may also participate in signal transduction systems that regulate cell growth. The family of plasma membrane Ca2+ channels includes L-type channels, which respond to membrane depolarization by generating a signal that opens the intracellular Ca2+ release channels. Calcium ion entry through L-type Ca2+ channels in the sinoatrial (SA) node contributes to pacemaker activity, whereas L-type Ca2+ channels in the atrioventricular (AV) node are essential for AV conduction. The T-type Ca2+ channels, another member of the family of plasma membrane Ca2+ channels, participate in pharmacomechanical coupling in smooth muscle. Opening of these channels in response to membrane depolarization participates in SA node pacemaker currents, but their role in the working cells of the atria and ventricle is less clear. Like the InsP3-activated intracellular Ca2+ release channels, T-type plasma membrane channels may regulate cell growth. Because most of the familiar Ca2+ channel blocking agents currently used in cardiology, such as nifedipine, verapamil and diltiazem, are selective for L-type Ca2+ channels, the recent development of drugs that selectively block T-type Ca2+ channels offers promise of new approaches to cardiovascular therapy.
Assuntos
Canais de Cálcio/fisiologia , Fenômenos Fisiológicos Cardiovasculares , Transdução de Sinais , Animais , Bloqueadores dos Canais de Cálcio/farmacologia , Canais de Cálcio/efeitos dos fármacos , HumanosRESUMO
It is now generally agreed that Na+-K+ adenosine triphosphatase (ATPase), a transport enzyme derived from the sarcolemmal sodium pump, is the primary site at which digitalis exerts its effects on the myocardial cell. Inhibition of the ability of this ion transport enzyme to catalyze Na+ efflux from the cell in exchange for K+ leads to both the therapeutic and toxic effects of the cardiac glycosides. The mechanism by which digitalis inhibits the sodium pump has been established in studies of Na+-K+ ATPase which show that the ability of cardiac glycosides to inhibit adenosine triphosphate (ATP)-supported transport of Na+ is reduced in the presence of elevated levels of K+. These studies explain the ability of hypokalemia to potentiate the effects of cardiac glycosides on the heart, and of high K+ concentrations to overcome the inhibition of sodium pump activity by the cardiac glycosides. Recent demonstrations that the positive inotropic effect of the cardiac glycosides is correlated with an increased intracellular Na+ provide strong evidence that these effects of digitalis to impair sodium efflux are responsible for the increased myocardial contractility caused by digitalis.
Assuntos
Glicosídeos Digitálicos/farmacologia , Canais Iônicos/enzimologia , Miocárdio/metabolismo , ATPase Trocadora de Sódio-Potássio/antagonistas & inibidores , Sódio/metabolismo , Trifosfato de Adenosina/farmacologia , Sítios de Ligação/efeitos dos fármacos , Cálcio/metabolismo , Glicosídeos Cardíacos/farmacologia , Humanos , Canais Iônicos/efeitos dos fármacos , Potenciais da Membrana/efeitos dos fármacos , Miocárdio/citologia , Potássio/metabolismo , ATPase Trocadora de Sódio-Potássio/fisiologiaRESUMO
Agents that increase cellular cyclic adenosine monophosphate (cyclic AMP) levels exert several effects on cardiac function. These include increases in heart rate and both the force of contraction and rate of relaxation. The latter effects, which might appear to be contradictory, actually represent essential components of the response needed to allow the heart to increase its stroke volume when heart rate is accelerated. This article reviews the mechanisms by which cyclic AMP exerts both contraction-promoting and relaxation-promoting effects in the integrated response of the heart to sympathetic stimulation.
Assuntos
AMP Cíclico/fisiologia , Contração Miocárdica , Miocárdio/metabolismo , Adenosina Trifosfatases/metabolismo , Cálcio/metabolismo , Proteínas de Ligação ao Cálcio/metabolismo , AMP Cíclico/biossíntese , Metabolismo Energético , Humanos , Canais Iônicos/metabolismo , Fosforilação , Proteínas Quinases/metabolismo , Sarcolema/enzimologia , Retículo Sarcoplasmático/metabolismo , Sódio/metabolismo , Troponina/metabolismo , Troponina IRESUMO
The past 25 years have seen an unprecedented growth in our understanding of the mechanisms responsible for the regulation of myocardial contractility. Beginning with a demonstration that myocardial contractility represents an important determinant of cardiac function, this quarter century has witnessed a series of attempts to explain length-independent changes in myocardial contractile function. Alterations in the properties of the cardiac contractile proteins and changes in the amount of calcium (Ca2+) available for binding to the contractile proteins during excitation-contraction coupling are now recognized as important biochemical bases for physiologic, pharmacologic and pathologic changes in myocardial contractility. Identification of the central role of Ca2+ in the initiation of the cardiac contractile process has made possible the analysis of the mechanisms by which several drugs alter myocardial contractile function, including the biochemical processes that allow beta-adrenergic agonists to enhance contractility. The rapid developments in this field since 1958 promise an even greater flow of new knowledge regarding the causes, prevention and treatment of heart failure provided that there is given adequate support for such research activities.