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1.
Int J Paediatr Dent ; 34(4): 474-484, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38124259

RESUMO

BACKGROUND: Paediatric rotary file systems have recently been developed for primary teeth use. AIM: To study the cleaning efficacies of two paediatric rotary endodontic files, the Prime PedoTM, and the Kedo-SG BlueTM against the standard H files. DESIGN: This in vitro study included 54 freshly extracted primary molars, which were randomised into three groups (n = 18 each) and were prepared using either Kedo-SG BlueTM, Prime PedoTM or hand H files after injecting methylene blue dye into the canals. Pre- and post-operative cone beam computerised tomography (CBCT) was performed to assess change in root canal volumes. Methylene blue dye removal from canals was assessed using stereomicroscopy, and canal cleanliness was examined by scanning electron microscopy (SEM). RESULTS: Both Prime PedoTM and Kedo-SG BlueTM files reduced significantly less dentine when compared with conventional hand filing with Prime PedoTM removing the least amount of dentine. No significant difference was found in median SEM scores among the groups in the cervical, middle and apical thirds of the roots. Stereomicroscopic assessment of root canal cleanliness using dye removal technique shows a statistically significant difference existing between Kedo-SG BlueTM and hand H files groups. CONCLUSION: Prime PedoTM removed the least amount of dentine. Kedo-SG BlueTM performed significantly better than conventional hand filing with H files when the root canal cleanliness was assessed.


Assuntos
Cavidade Pulpar , Microscopia Eletrônica de Varredura , Dente Molar , Preparo de Canal Radicular , Dente Decíduo , Humanos , Preparo de Canal Radicular/instrumentação , Preparo de Canal Radicular/métodos , Técnicas In Vitro , Instrumentos Odontológicos , Tomografia Computadorizada de Feixe Cônico , Desenho de Equipamento , Azul de Metileno
2.
Neurol Sci ; 44(6): 1959-1968, 2023 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-36729186

RESUMO

BACKGROUND: Subacute sclerosing panencephalitis (SSPE) is a devastating brain disease caused by persistent infection by the measles virus. Several cases of SSPE in pregnant ladies have been described. This systematic review is focused on maternal and foetal outcomes among pregnant women with SSPE. METHODS: We searched four databases (PubMed, Embase, Scopus, and Google Scholar). We reviewed all relevant cases, published until 14 August 2022. The review was conducted following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. The protocol was registered with PROSPERO (CRD42022348630). The search items that we used were "((Pregnancy) OR (delivery)) AND (Subacute sclerosing panencephalitis (SSPE))". Dyken's criteria were used for the diagnosis of SSPE in pregnant women. The extracted data was recorded in an Excel sheet. The Joanna Briggs Institute Critical Appraisal tool for case reports was used to assess the quality of published cases. RESULTS: We came across 19 reports describing details of 21 cases. The age of SSPE-affected women varied from 14 to 34 years (mean 23 years). In the majority (n=14), clinical manifestations were started in the antepartum period. Nine pregnant SSPE women presented with vision loss. After delivery, 13 SSPE-affected women died. On the contrary, 15 foetuses, though the majority were preterm, were alive. Five foetuses either died soon after birth or were still-born. CONCLUSION: In conclusion, SSPE in pregnancy is often missed, as it mimics eclampsia. SSPE in pregnancy usually has a devastating course. Universal early childhood measles vaccination is the only way to fight this menace.


Assuntos
Sarampo , Panencefalite Esclerosante Subaguda , Recém-Nascido , Feminino , Humanos , Pré-Escolar , Gravidez , Adolescente , Adulto Jovem , Adulto , Panencefalite Esclerosante Subaguda/diagnóstico , Panencefalite Esclerosante Subaguda/etiologia , Gestantes , Vírus do Sarampo , Transtornos da Visão , Família , Sarampo/complicações
3.
Neurobiol Dis ; 169: 105722, 2022 07.
Artigo em Inglês | MEDLINE | ID: mdl-35405261

RESUMO

The 2021 VCP Scientific Conference took place virtually from September 9-10, 2021. This conference, planned and organized by the nonprofit patient advocacy group Cure VCP Disease, Inc. (https://www.curevcp.org), was the first VCP focused meeting since the 215th ENMC International Workshop VCP-related multi-system proteinopathy in 2016 (Evangelista et al., 2016). Mutations in VCP cause a complex and heterogenous disease termed inclusion body myopathy (IBM) with Paget's disease of the bone (PDB) and frontotemporal dementia (FTD) (IBMPFD), or multisystem proteinopathy 1 (MSP-1) Kimonis (n.d.), Kovach et al. (2001), Kimonis et al. (2000). In addition, VCP mutations also cause other age-related neurodegenerative disorders including amyptrophic lateral sclerosis (ALS), Parkinsonism, Charcot-Marie type II-B, vacuolar tauopathy among others (Korb et al., 2022). The objectives of this conference were as follows: (1) to provide a forum that facilitates sharing of published and unpublished information on physiological roles of p97/VCP, and on how mutations of VCP lead to diseases; (2) to bolster understanding of mechanisms involved in p97/VCP-relevant diseases and to enable identification of therapeutics to treat these conditions; (3) to identify gaps and barriers of further discoveries and translational research in the p97/VCP field; (4) to set a concrete basic and translational research agenda for future studies including crucial discussions on biomarker discoveries and patient longitudinal studies to facilitate near-term clinical trials; (5) to accelerate cross-disciplinary research collaborations among p97/VCP researchers; (6) to enable attendees to learn about new tools and reagents with the potential to facilitate p97/VCP research; (7) to assist trainees in propelling their research and to foster mentorship from leaders in the field; and (8) to promote diversity and inclusion of under-represented minorities in p97/VCP research as diversity is critically important for strong scientific research. Given the range of topics, the VCP Scientific Conference brought together over one hundred and forty individuals representing a diverse group of research scientists, trainees, medical practitioners, industry representatives, and patient advocates. Twenty-five institutions with individuals from thirteen countries attended this virtual meeting. In this report, we summarize the major topics presented at this conference by a range of experts.


Assuntos
Demência Frontotemporal , Miosite de Corpos de Inclusão , Proteínas de Ciclo Celular/genética , Proteínas de Ciclo Celular/metabolismo , Demência Frontotemporal/genética , Demência Frontotemporal/terapia , Humanos , Proteína 1 de Superfície de Merozoito/genética , Distrofia Muscular do Cíngulo dos Membros , Mutação , Miosite de Corpos de Inclusão/genética , Miosite de Corpos de Inclusão/terapia , Osteíte Deformante , Proteína com Valosina/genética
4.
Med J Armed Forces India ; 77(Suppl 1): S22-S30, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33612928

RESUMO

The changing paradigm of dental education in India has led its way to the development of Competency-Based Curriculum (CBC). This article describes the process of developing CBC in the specialty of Pediatric Dentistry under the initiative of Dental Council of India. Rationale behind CBC development is to bring uniform system of education for improving oral health outcomes of the society in long term. The process of CBC development was a collaborative teamwork, planned meticulously with predefined outline, tasks and timelines. Workflow involved identification of curricular content, defining program goals, outlining competencies, assigning them domains / levels of clinical competence, priority, educational strategies, assessment practices, integration and numbers needed for certification in selected competencies. Early clinical exposure was introduced in CBC. The final content was validated and submitted to the Council. CBC output can be summarized as competencies with fair share of all domains, levels, prioritization and integration. It is characterized by flexibility for choosing educational strategies and assessment practices. It opens up ways for global competition. However, it still has some inherent weaknesses like diverging learning paths, time constraints and number chasing. CBC can further have more academic flexibility and develop toward an outcome-based approach. Faculty preparedness and acceptability shall be the biggest challenges in CBC implementation besides resources' availability, support from leadership and acceptability from our primary stakeholders, our learners. CBC is the beginning of evidence-based delivery of education in dentistry. An effective implementation of CBC in current form would result in increased numbers of competent oral healthcare professionals for the society.

5.
Biochim Biophys Acta Mol Cell Res ; 1865(8): 1088-1104, 2018 08.
Artigo em Inglês | MEDLINE | ID: mdl-29753763

RESUMO

Lamins constitute the major architectural proteins of the nuclear lamina that help in maintaining nuclear organization. Mutations in lamins are associated with diverse degenerative diseases, collectively termed laminopathies. HECW2, a HECT-type E3 ubiquitin ligase, is transcriptionally upregulated in HeLa cells expressing Emery-Dreifuss muscular dystrophy-causing-lamin A mutants. However, the role of HECW2 upregulation in mediating downstream effects in lamin mutant-expressing cells was previously unexplored. Here, we show that HECW2 interacts with two lamin A-binding proteins, proliferating cell nuclear antigen (PCNA), via a canonical PCNA-interacting protein (PIP) motif, and lamin B1. HECW2 mediates their ubiquitination and targets them for proteasomal degradation. Cells expressing lamin A mutants G232E and Q294P, in which HECW2 is upregulated, show increased proteasomal degradation of PCNA and lamin B1 most likely mediated by HECW2. Our findings establish HECW2 as an E3 ubiquitin ligase for PCNA and lamin B1 which regulates their levels in laminopathic cells. We also found that HECW2 interacts with wild-type lamin A and ubiquitinates it and this interaction is reduced in case of lamin mutants G232E and Q294P. Our findings suggest that interplay among HECW2, lamin A, PCNA, and lamin B1 determines their respective homeostatic levels in the cell and dysregulation of these interactions may contribute to the pathogenicity of laminopathies.


Assuntos
Lamina Tipo B/metabolismo , Antígeno Nuclear de Célula em Proliferação/metabolismo , Ubiquitina-Proteína Ligases/química , Ubiquitina-Proteína Ligases/metabolismo , Sítios de Ligação , Células HEK293 , Humanos , Lamina Tipo A/genética , Lamina Tipo B/química , Lamina Tipo B/genética , Distrofia Muscular de Emery-Dreifuss/genética , Mutação , Antígeno Nuclear de Célula em Proliferação/química , Complexo de Endopeptidases do Proteassoma/química , Complexo de Endopeptidases do Proteassoma/metabolismo , Proteólise , Ubiquitinação
6.
Biochem Biophys Res Commun ; 503(4): 2478-2484, 2018 09 18.
Artigo em Inglês | MEDLINE | ID: mdl-30208514

RESUMO

Heterochromatin protein 1 (HP1) isoforms are conserved non-histone chromatin-binding proteins that play an important role in packaging of heterochromatin and gene regulation. The functions of HP1 isoforms α and ß are influenced by the nuclear lamins. Cells expressing disease-causing lamin A rod domain mutants show depletion of HP1α and ß and E3 ubiquitin ligase HECW2 is upregulated in these cells. However, the role of HECW2 in the turnover of HP1 proteins has been hitherto unexplored. Here, we show that HECW2 interacts with HP1 isoforms α and ß but not HP1γ. Ectopic expression of HECW2 causes the ubiquitination of HP1α and ß, thereby targeting them for proteasomal degradation. Downregulation of HECW2 increases their steady-state levels indicating its role in their homeostatic regulation. Our results give important insights into the mechanism of proteasomal degradation of HP1 proteins in laminopathic cells.


Assuntos
Proteínas Cromossômicas não Histona/metabolismo , Complexo de Endopeptidases do Proteassoma/metabolismo , Ubiquitina-Proteína Ligases/fisiologia , Homólogo 5 da Proteína Cromobox , Humanos , Isoformas de Proteínas , Ubiquitinação
7.
BMJ Case Rep ; 17(7)2024 Jul 16.
Artigo em Inglês | MEDLINE | ID: mdl-39013627

RESUMO

Odontoma is the most common odontogenic tumour derived from both epithelial and mesenchymal components of the tooth-forming apparatus. It is commonly diagnosed in the second and third decades of life when a radiograph is taken for some other purpose, as most cases are asymptomatic. This case involves a young boy, with the chief complaint of pain and swelling in the lower left back region. An intraoral examination revealed a carious and hypoplastic left permanent mandibular first molar. Although the molar was suspected as the source of his symptoms, radiographic imaging revealed multiple odontomas and missing second and third molar tooth buds. This case highlights the pathological effects of odontomas on surrounding teeth, including the malformation of the first molar and aplasia of the second and third molars. The sole management depends on the early diagnosis, histopathological examination to rule out malignancy and conservative surgical excision of these tissues.


Assuntos
Odontoma , Humanos , Masculino , Odontoma/patologia , Odontoma/cirurgia , Odontoma/diagnóstico , Odontoma/diagnóstico por imagem , Dente Molar/patologia , Dente Molar/diagnóstico por imagem , Neoplasias Mandibulares/patologia , Neoplasias Mandibulares/diagnóstico por imagem , Neoplasias Mandibulares/cirurgia , Neoplasias Mandibulares/diagnóstico , Criança , Diagnóstico Diferencial
8.
Quintessence Int ; 55(5): 372-378, 2024 05 30.
Artigo em Inglês | MEDLINE | ID: mdl-38502154

RESUMO

OBJECTIVE: To compare the effect of planning interventions on self-reported changes in parents supervised oral health-related behaviors (OHRBs) and associated clinical oral health parameters for primary school-age children. METHOD AND MATERIALS: In total, 110 parent-child pairs (children aged 3 to 8 years) were randomly assigned to either of the two groups. In the "action planning" group, parent participants of the pair were asked to make an "action plan" using the "how, when, where" format for their child OHRBs. In the "implementation intention" group, parents were asked to form an "if-then plan" to improve OHRBs for their child. Self-reported changes on target OHRBs, change in plaque scores, change in plaque stagnation areas, and change in caries status of tooth surfaces were observed at 2, 8, and 12 weeks. RESULTS: Overall OHRBs scores changed significantly from baseline to 12 weeks for both interventions. The scores were significantly better with "if-then" planning than "action planning" (z = 4, P < .001) at 12 weeks. Plaque scores also changed significantly from baseline to 12 weeks for both interventions, and improved significantly more with "if-then" planning (16.20 ± 5.24) than "action planning" (50.66 ± 11.24) at 12 weeks. The number of plaque stagnation areas also changed significantly from baseline to 12 weeks for both interventions, and improved significantly more with "if-then" planning at 12 weeks (12.80 ± 5.33) than "action planning" (42.76 ± 10.34) (t = -11.55, P < .001). There was significant change in the caries status of sound tooth surfaces with "action planning" at 12 weeks (z = 116.50, P = .023). There were no new caries lesions reported with "if-then" planning at 12 weeks. CONCLUSION: The study observed significant improvement in OHRBs and associated oral health parameters with planning interventions, preferring "if-then" planning over "action planning." It also elicited significant barriers to behaviors in action.


Assuntos
Comportamentos Relacionados com a Saúde , Saúde Bucal , Pais , Humanos , Criança , Feminino , Masculino , Pré-Escolar , Pais/psicologia , Pais/educação , Cárie Dentária/prevenção & controle , Autorrelato
9.
J Oral Biol Craniofac Res ; 14(5): 662-666, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39309314

RESUMO

Purpose: Competence building in interpersonal communication (IPC) between undergraduate students and patients requires deep understanding of the factors influencing their interaction. These factors, contextual to our clinical setup were explored from multisource perspectives to understand the perceived barriers in IPC. Method: Qualitative hermeneutic phenomenological analysis was done for patient's semi-structured interviews (n = 16); interns (n = 15) and faculty (n = 14) unstructured focus group discussions. Three independent authors transcribed the data, derived codes and themes individually, and then collated and reflected on them to draw conclusions. Different collection methods, multisource perspectives, and multilevel analysis provided for triangulation and validation of the study results. Results: This study's multisource feedback identified seven inter-dependent barriers to interpersonal communication between students and patients. They were time constraints, lack of trust and respect, cultural diversity, loco-regional linguistic differences, lack of empathy, illiteracy, and financial constraints. Conclusions: The study concluded by identifying the unique set of barriers preventing interpersonal communication in our clinical microsystem. Addressing these contextual factors by preparing training modules and workshops would prepare undergraduate students for patient-centered care and partnership.

10.
Artigo em Inglês | MEDLINE | ID: mdl-38427769

RESUMO

ABSTRACT: Amyloidosis is a relatively rare condition with an array of complex pathophysiology. Localized amyloidosis is a rare and benign condition that practically never results in any clinical repercussions in the head and neck area. Multiple soft nodules of the tongue, lip, and cheek are the most commonly described defining characteristics of localized oral amyloidosis. These nodules originate due to the proliferation of abnormally folded protein aggregates in the body's extracellular tissue compartments, which destroy organ structure and function. Herein, we address the case of a female infant aged one with a smooth nodule in the labial mucosa who was diagnosed with primary localized amyloidosis. When a patient is diagnosed with amyloidosis of the oral mucosa, the possibility of systemic amyloidosis or an underlying plasma cell dyscrasia must be ruled out. Surgical treatment may be beneficial for eliminating any functional impairment if primary localized amyloidosis is established.

11.
Int J Clin Pediatr Dent ; 17(7): 842-850, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-39372520

RESUMO

The aims of dental professional courses are to prepare dental healthcare providers to manage common dental diseases, perform surgeries, and offer prevention. They should also be equipped to understand the needs of the society they are serving and willing to amend their skills. As they are expected to be a part of a team providing health care, they should have the requisite leadership and managerial skills for leading the team to serve its objectives in the best possible way. Thus, with changing times, there is a need to bring reforms in dental curricula. Traditional classroom teaching is now shifting to a competency-based education system across the globe. The Dental Council of India (DCI), as a dental health regulator in the country, implements reforms periodically in an attempt to further strengthen the training process and bring quality improvement in dental education. A process of redesigning the curriculum started a couple of years ago. The technical team supporting this work brainstormed the need for such reforms and studied the existing pattern of undergraduate curricula in other developed countries. It was found that many countries are following outcome-based teaching-learning methods. There are several institutions and professional associations proposing recommendations on curricular reforms, and India also suggests following the same. The new education policy (NEP) of the Government of India (GoI) is also in sync with the changes proposed to the DCI. Though such changes are challenging and require time to strategize and implement, it is essential to have reforms in curriculum, especially related to methods of teaching-learning and assessment, and for this, the dental faculty needs to be trained. How to cite this article: Deshpande AN, Mathur VP, Lele GS, et al. Identifying Needs and Preparing for Curriculum Changes in Indian Dental Education. Int J Clin Pediatr Dent 2024;17(7):842-850.

12.
J Dent Anesth Pain Med ; 23(2): 69-81, 2023 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-37034838

RESUMO

Background: In order to assess the effectiveness of various analgesio-sedative combinations for pain relief and sedation in pediatric dental patients, a thorough evaluation of clinical studies and patient outcomes is necessary. Methods: A total of 128 healthy, uncooperative pediatric dental patients were randomly allocated to receive one of the four combinations of drugs via the intranasal (IN) route: Group I received midazolam-ketamine (MK), Group II received dexmedetomidine-ketamine (DK), Group III received midazolam-fentanyl (MF), and Group IV received dexmedetomidine-fentanyl (DF) in a parallel-arm study design. The efficacy and safety of the combinations were evaluated using different parameters. Results: The onset of sedation was significantly faster in the DF group than in the DK, MF, and MK groups (P < 0.001). The depth of sedation was significantly higher in the DK and DF groups than in the MK and MF groups (P < 0.01). DK and DF produced significant intra- and postoperative analgesia when compared with combinations of MK and MF. No significant adverse events were observed for any of the combinations. Conclusions: The DK and DF groups showed potential as analgesio-sedatives in view of their anxiolytic and analgesic effects.

13.
J Oral Biol Craniofac Res ; 11(2): 334-343, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33786297

RESUMO

PURPOSE: The aim of this article was to systematically review the available literature on patient specific total temporomandibular joint total joint replacement (PS-TMJR) implants for their biomaterial, designs, fabrication techniques and their outcomes. METHODS: A literature review was conducted using PubMed, and science direct databases using the key words three-dimensional printing, 3D printing, CAD CAM, computer aided designing, computer aided manufacturing, additive technology, custom made implants, patient specific implants in combination with Temporomandibular joint, TMJ surgery. RESULTS: The search revealed 2760 articles, of which 374 were in English and discussed TMJ reconstruction. Further filtering shortlisted 74 articles that discussed PS-TMJR. Duplicates were removed and additional added from article references. 39 articles describing biomaterial, designing and fabrication of PS-TMJR implants and their outcomes were selected for analysis. CONCLUSIONS: Although PS-TMJR implants allow a better anatomical fit, improved fixation, and safeguard various structures such as the inferior alveolar nerve, they vary in designs, material and fabrication techniques. However, PS-TMJR printed with SLM and EBM technologies have yet to be compared with the conventional ones in terms of mechanical strength, and clinical outcome. With emerging bioprinting technologies, even newer biomaterials should be considered for 3D printing of PS-TMJR devices designed to achieve harmony in function between the joint device, bone and masticatory muscles.

14.
Int J Clin Pediatr Dent ; 13(Suppl 1): S1-S13, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-34434008

RESUMO

AIM AND OBJECTIVE: This study assesses the efficacy of mineral trioxide aggregate (MTA), biodentine, and calcium phosphate cement (CPC) as single visit apexification agents for nonvital immature permanent teeth, both clinically and radiographically. MATERIALS AND METHODS: The study was conducted as a double-blinded randomized, controlled clinical trial after approval of the Institutional Ethical Committee of King George's Medical University, Lucknow, Uttar Pradesh, India, the approval letter (Ref. no. 81st ECM II B-Thesis/P24). A total of 60 patients in the age group of 6-15 years, fulfilling all the inclusion and exclusion criteria were enrolled for the study. Patients were randomly divided into three groups having 20 in each group. RESULTS: On the basis of present study, it can hence, be inferred that clinical success for MTA, biodentine and calcium phosphate cement in apexification was 100%. The radiographic outcomes of calcium phosphate cement showed better results as compared to MTA and biodentine at 9 months of follow-up periods. CONCLUSION: These finding suggest that calcium phosphate cement can be used as a substitute for MTA and biodentine because of its comparable clinical and superior radiographic success. HOW TO CITE THIS ARTICLE: Yadav A, Chak RK, Khanna R. Comparative Evaluation of MTA, Biodentine and Calcium Phosphate Cement in Single Visit Apexification Procedure for Nonvital Immature Permanent Teeth: A Randomized Controlled Trial. Int J Clin Pediatr Dent 2020;13(S-1):S1-S13.

15.
J Indian Soc Pedod Prev Dent ; 38(3): 293-303, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33004729

RESUMO

INTRODUCTION: Basic behavioral guidance (BBG) skills help in delivery of quality health care in pediatric dentistry. The complex nature of these skills, warrants analyzing actions performed. An appropriate scientific way to do this is by "reflection." Hence, the present study was designed to introduce "reflection of action" as means for learning BBG skills by undergraduates in pediatric dentistry. METHODS: Participants (dental undergraduate) performed oral prophylaxis (two visits) in pediatric patients (age 3-7 years) with application of BBG skills, under video recording. They were instructed regarding "reflection on action." The learners then reviewed own videos and wrote reflections. Reflections were assessed on Boud's 4R framework and feedback was given by the faculty. Reflective writing was repeated for a second visit. Knowledge of the learners in using reflections for learning was assessed by retrospective pretest posttest questionnaire. Video recordings were scored for BBG skills. Acceptability of the intervention was addressed by satisfaction questionnaire. RESULTS: There was a significant improvement in the knowledge of participants in using reflections for learning these skills. All participants were able to "revisit" (R1 level under Boud's 4R framework) patient encounter in their written reflections. Sixteen participants exhibited shift toward higher levels in the next visit. Video scores of learners also improved significantly over both visits. Students were satisfied with the content, delivery, and relevance of the new educational intervention. CONCLUSION: The strong need of improving BBG skills in pediatric dentistry was met by "reflection on action." There was improvement in the knowledge of students in using reflections for learning and application of behavior guidance skills and was well accepted.


Assuntos
Aprendizagem , Odontopediatria , Criança , Humanos , Estudos Retrospectivos , Inquéritos e Questionários , Redação
16.
Quintessence Int ; 51(6): 448-455, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32368763

RESUMO

OBJECTIVE: Qualitative developmental defects of enamel (DDEs) are characterized by an intact hypermineralized surface enamel layer and a porous subsurface hypomineralized layer. This structure is similar to natural early caries lesions, which are effectively arrested by resin infiltration, a micro-invasive clinical procedure. An additional reported effect in early enamel caries is recovery of enamel translucency. Due to structural similarities, a similar mechanism is expected in qualitative DDEs. The objective of the present study was to observe quantitative color masking of qualitative DDEs when subjected to resin infiltration. METHOD AND MATERIALS: A total of 70 selected enamel defects were subjected to resin infiltration in a nonrandomized study design. Each defect was subjected to photographic imaging using a digital camera both pre- and post-intervention. Each image was assessed using ImageJ digital software (National Institutes of Health) for CIE L*a*b coordinates. RESULTS: There were significant changes observed in L*a*b coordinates of all DDEs, when subjected to resin infiltration. CONCLUSION: The resin infiltration intervention was successful in masking the "white" opaque discolorations of developmentally affected enamel.


Assuntos
Cárie Dentária , Doenças Dentárias , Cor , Esmalte Dentário , Humanos , Resinas Sintéticas
17.
BMJ Case Rep ; 12(7)2019 Jul 08.
Artigo em Inglês | MEDLINE | ID: mdl-31289158

RESUMO

The Bernard-Soulier syndrome also known as hemorrhagioparous thrombocytic dystrophy, is a rare autosomal recessive bleeding disorder. Macrothrombocytopenia and increased bleeding time are the two main features of this syndrome. There is a reduced adherence of giant platelets to the subendothelium due to a deficiency of glycoprotein GPIb-V-IX complex. Routine dental extraction in a patient with a bleeding disorder can lead to life-threatening complications and there are very few specific protocols for the same. Here, we present a case report describing haemostasis management during dental extraction in a 7-year-old female child, suffering from this syndrome. It was observed that the preoperative and postoperative human leucocyte antigen-matched platelet transfusion, systemic intraoperative tranexamic acid and minimally traumatic procedure improved bleeding control and management.


Assuntos
Síndrome de Bernard-Soulier/complicações , Hemostasia/efeitos dos fármacos , Extração Dentária/efeitos adversos , Administração Oral , Síndrome de Bernard-Soulier/diagnóstico , Plaquetas/patologia , Criança , Diagnóstico Diferencial , Feminino , Humanos , Transfusão de Plaquetas/métodos , Cuidados Pré-Operatórios , Ácido Tranexâmico/administração & dosagem , Ácido Tranexâmico/uso terapêutico , Resultado do Tratamento
18.
FEBS J ; 285(12): 2243-2262, 2018 06.
Artigo em Inglês | MEDLINE | ID: mdl-29676528

RESUMO

Lamins are key nuclear proteins which are important for maintaining nuclear structure and function. Mutations in lamins cause a spectrum of genetic diseases termed as laminopathies. RING finger containing E3 ubiquitin ligase, RNF123, is transcriptionally upregulated in cells expressing rod domain lamin A mutations. However, the functional relevance of RNF123 in laminopathic cells is not clear. Using a mass spectrometry-based approach, we identified lamins and lamin-binding proteins retinoblastoma protein (pRb), lamina-associated polypeptide 2α (LAP2α), and emerin as RNF123-interacting proteins. We determined that RNF123 mediated the ubiquitination of these proteins and caused the proteasomal degradation of pRb, LAP2α, and lamin B1. Furthermore, these proteins were also targeted for proteasomal degradation in cells expressing lamin A rod domain mutants G232E, Q294P, and R386K. Overexpression of RNF123 resulted in delayed transit through the S-phase which was alleviated by coexpression of pRb or LAP2α. Our findings imply that RNF123-mediated ubiquitination of lamin-binding proteins may contribute to disease-causing mechanisms in laminopathies by depletion of key nuclear proteins and defects in cell cycle kinetics.


Assuntos
Proteínas de Ligação a DNA/metabolismo , Lamina Tipo B/metabolismo , Proteínas de Membrana/metabolismo , Proteínas Nucleares/metabolismo , Processamento de Proteína Pós-Traducional , Proteína do Retinoblastoma/metabolismo , Ubiquitina-Proteína Ligases/metabolismo , Núcleo Celular/metabolismo , Proliferação de Células , Clonagem Molecular , Proteínas de Ligação a DNA/genética , Expressão Gênica , Genes Reporter , Proteínas de Fluorescência Verde , Células HEK293 , Células HeLa , Humanos , Lamina Tipo A/genética , Lamina Tipo A/metabolismo , Lamina Tipo B/genética , Espectrometria de Massas , Proteínas de Membrana/genética , Mutação , Proteínas Nucleares/genética , Plasmídeos/química , Plasmídeos/metabolismo , Complexo de Endopeptidases do Proteassoma/metabolismo , Proteólise , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Proteína do Retinoblastoma/genética , Ubiquitina-Proteína Ligases/genética , Ubiquitinação
19.
Saudi Dent J ; 30(1): 53-62, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30166872

RESUMO

AIM: The aim of the present study was to explore the adjunctive use of Acacia arabica gel in the treatment of chronic periodontitis. METHODS: Single centre, randomised, triple blind, controlled trial on mild to moderate chronic periodontitis patients; Group I (SRP + Acacia arabica, n = 40) and Group II (SRP + placebo, n = 40); were analysed for clinical improvements in periodontal pocket depth (PPD) and clinical attachment levels (CAL) at baseline, 15 and 90 days on application of gels. Gingival index and plaque index were assessed as secondary parameters. RESULTS: Statistically significant PPD reduction (p < .05) and CAL gain (p < .05) was observed with use of Acacia arabica gel. The reduction in sites with moderate PPD was observed more among Group I than Group II and the difference was statistically significant (p = .001). Secondary outcome variables; Plaque Index and Gingival Index showed better resolution with Acacia arabica gel. CONCLUSION: Acacia arabica leads to better clinical outcomes in patients with mild to moderate chronic periodontitis with effective antiplaque and anti-gingivitis action. It may be recommended adjunct to SRP for maintenance in patients with mild to moderate chronic periodontitis.

20.
Crit Care Res Pract ; 2017: 1348372, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-29181195

RESUMO

Nosocomial infections in critically ill/ventilated patients result from bacterial load in oropharyngeal regions. Oral decontamination serves as the easiest effective means of controlling infections. Knowledge, attitude, and practices followed by healthcare personnel in intensive care settings need to be assessed to implement concrete measures in health-care. Survey questionnaire was constructed and implemented following its validation on seventy nursing and paramedical staff working in government and private intensive care units throughout Lucknow city. 21-item questionnaire consisted of three parts of seven questions each. 78% of respondents had knowledge regarding oral care and its importance in critical settings but 44% of respondents considered it to be unpleasant task. 36% of respondents claimed to have provided oral care to all patients in ICU. Uniform guidelines for translation of oral healthcare in ICU settings are not being implemented. Previous studies in literature from various geographic diverse regions also point out to similar lacunae. Based on present survey, most respondents were aware of importance of oral care with protocols covered in academic curriculum. Attitude towards oral care is positive but respondents feel a need for specialised training. Practice for oral care is not sufficient and needs improvement and proper implementation.

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