Comparison of the action of transforming growth factor-beta 1 and interleukin-1 beta on matrix metabolism in the culture of porcine endothelial cells.

The purpose of the experiment was to evaluate the metabolism of the connective tissue (collagen and proteoglycans) in the endothelial cell culture (enzymatically isolated from porcine aortas). The endothelial cells were activated by 1-10 ng/ml TGF-beta 1 and 250 pg/ml IL-1 beta together and separately. Intensity of proteoglycan synthesis has been evaluated by using radioactive [35S]-sodium sulfate whereas the intensity of collagen was evaluated by incorporation of [3H]-proline. The following conclusions have been drawn as the result of the experiment: 1) TGF-beta 1 stimulates the synthesis of proteoglycans and collagen in endothelial cell culture, whereas IL-1 beta has the opposite action; and 2) TGF-beta 1 reduces IL-1 beta action which hampers the synthesis of proteoglycans and collagen, provided that the culture's preincubation with TGF-beta 1 precedes the incubation with IL-1 beta.

[The influence of heart infarction on the concentration of aminoterminal type III procollagen peptide in blood serum]. / Wplyw zawalu miesnia sercowego na stezenie aminoterminalnego peptydu prokolagenu typu III w surowicy krwi.

UNLABELLED: The reconstructive processes in a heart infarction pertain also the fibrous tissue sceleton, of which the main element are collagen fibres. The aim of the study was determination of variability of a specific collagen synthesis marker, i.e. the serum aminoterminal type III procollagen peptide (PIIINP) and its correlation with hydroxyproline (HP), hydroxylysin (HL) concentration as well as activity of creatine kinase (CK) and aspartate aminotransferase (AspAT) in serum after heart infarction. The investigations were carried out in 30 patients with a heart infarction with Q wave (group I), in 20 subjects with a heart infarction without Q wave (group II) and in 30 healthy subjects, comprising the C group. All these parameters were determined on the 1st, 2nd, 3rd, 5th and 10th day after onset of infarction. In comparison to the C group, in group I on the 1st day the PIIINP concentration was 3-fold higher and in group II it 2 1/2-fold higher (C = 4.3 +/- 1.8; I = 14.2 +/- 3.9; II = 10.4 +/- 2.0 micrograms/l; p < 0.001). In group I even on the 10th day the concentration did not return to normal values, instead in group II it reached the values x + SD in C group after 5 days. There was no significant correlation between concentration of PIIINP and all other examined parameters stated. Heart infarction with a Q wave caused a relatively highest (% of mean values in C group) increase in CK and AspAT activity, PIIINP held a medial position, while HP and HL the lowest. After infarction without a Q wave the relative elevation of PIIINP concentration was near to the AspAT and HP increase. CONCLUSIONS: 1. the differences of serum PIIINP concentration are probably resulted by the magnitude of heart infarction. 2. During 10 days after the onset of heart infarction the serum concentration of PIIINP does not show any correlation with HP, HL and enzymatic heart infarct markers. 3. The results indirectly show, that the scar formation after heart infarction begins already on the first day.

[Effect of heart infarction on levels of type I procollagen carboxyterminal peptide in blood serum]. / Wplyw zawalu miesnia sercowego na stezenie karboksyterminalnego peptydu prokolagenu typu I w surowicy krwi.

The aim of the study was determination of the influence of heart infarction on the blood serum level of type I procollagen carboxyterminal peptide (PICP) and its covariability with concentration of hydroxyproline (HP) and hydroxylysine (HL) as well as activity of creatine kinase (CK) and aspartate transferase (AspAT). The investigations were carried out in 30 patients with a heart infarction with Q wave (group I) and in 20 subjects with a heart infarction without Q wave. The control group comprised 30 healthy subjects. The determination of all parameters was performed on the 1st, 2nd, 3rd, 5th and 10th day after the infarct onset. On the 1st day of heart infarction, the concentration of PICP in serum was (x +/- SD): gr. I-235 +/- 33, gr. II-209 +/- 8, gr. C-65 +/- 17 micrograms/l. There was no co-variability of PICP concentration and the values of all other determined parametres. The authors conclude: 1. the increase of serum PICP concentration is connected probably with the magnitude of heart infarction, 2. the reconstruction process of the heart fibrous tissue and scar formation begins already on the first day of infarction onset, 3. during 10 days after infarction onset the serum PICP concentration does not correlate with HP, HL as well as the enzymatic heart infarct indices, namely CK and AspAT.