Recurrence of Gonyaulax polygramma bloom in the southeastern Arabian Sea.

Gonyaulax polygramma, a bloom-forming dinoflagellate, has been repeatedly observed along the southeastern Arabian Sea in recent years. During our study in October 2021, a patch of reddish-brown water was observed in the nearshore waters off Kannur (southwest coast of India) and later identified as Gonyaulax polygramma using scanning electron microscopy (SEM) and HPLC-based phytoplankton marker pigments. Gonyaulax polygramma accounted for 99.4% of the phytoplankton abundance at the bloom location, with high concentrations of peridinin and chlorophyll-a at the study site. High concentration of SiO42- was observed at the bloom site, while other nutrients were lower than the previously reported values. The bloom of Gonyaulax polygramma also resulted in high concentrations of dimethylsulfide, an anti-greenhouse gas, at the bloom site. In addition to onsite observation, Sentinel-3 satellite data was also used in the detection and validation of the observed bloom using the NDCI index. From the satellite image, it was evident that the bloom persisted at the mouth of the rivers during the study period. Since the red tide of Gonyaulax polygramma has been observed recurrently in the southeastern Arabian Sea, it is proposed to use satellites to detect and monitor the bloom on a routine basis.

Organic carbon dynamics in the continental shelf waters of the eastern Arabian Sea.

The seasonal and spatial distribution of total organic carbon (TOC) is presented for the coastal waters of the eastern Arabian Sea, which experiences seasonal suboxia during the late southwest monsoon (SWM). This study reveals that high TOC was observed off Kochi as compared to Goa and Mangalore transects, and may be attributed to stronger upwelling along the Kerala coast. This is also supported by the excess carbon due to upwelling during the late SWM that varied from 37 µM (Goa), 39 µM (Mangalore), to 51 µM (Kochi). Our seasonal data from 2014 to 2020 at the Goa transect indicates that high TOC is seen during late SWM to fall inter monsoon (FIM) and between the late northeast monsoon (NEM) to the early spring inter monsoon (SIM). The high TOC concentrations and C/N ratios observed during the FIM are a combination of high primary production, the buildup of remnant organic matter from the previous season (due to prevailing low oxygen conditions), accumulation of refractory organic carbon, and release from diatoms (especially Chaetoceros sp.). Inter-annual variations indicate that phytoplankton blooms resulted in higher TOC concentrations, especially during the year 2020. Based on a comparison with an Elnino-Southern Oscillation (ENSO) year (2015), we can infer that the partitioning of carbon may increase from particulate to dissolved phase in future warming scenarios.

Sea foam-associated pathogenic bacteria along the west coast of India.

Anthropogenic activities release effluents containing nutrients and pathogenic bacteria that change the characteristics of coastal ecosystems. An important type of marine pollution which has occurred in 3 different states in India during 2019 is sea foam. Sea foam was found on Hole beach, Goa (Lat: 15.404° N, Long: 73.787° E), where nutrients (NO3- = 137 µM and organic nitrogen = 121 µM) from a garbage dumpyard are released directly via streams/gutters to coastal waters. This resulted in a bloom of the diatom Thalassiosira pseudonana, associated with high concentration of total organic carbon and fucoxanthin. Decay of this bloom along with strong agitation due to rocks and wave action resulted in sea foam. We isolated foam-associated bacteria and identified pathogenic bacteria including Enterobacter cancerogenus through 16S rRNA gene sequencing. Such foam-associated pathogenic bacteria, could be antibiotic resistant, and may have adverse effects on human health. This can also hamper the tourism industry of a small state like Goa that relies heavily on tourism.

Optical characteristics of colored dissolved organic matter during blooms of Trichodesmium in the coastal waters off Goa.

Trichodesmium, a marine cyanobacterium, plays a significant role in the global nitrogen cycle due to its nitrogen fixing ability. Large patches of Trichodesmium blooms were observed in the coastal waters, off Goa during spring intermonsoon (SIM) of 2014-2018. Zeaxanthin was the dominant pigment in the bloom region. Here, we present the spectral absorption and fluorescence characteristics of colored dissolved organic matter (CDOM) during these blooms. CDOM concentration was much higher in the bloom patches as compared with nonbloom regions. During the bloom spectral CDOM absorption had distinct peaks in the UV region due to the presence of UV-absorbing/screening compounds, mycosporine-like amino acids (MAAs) and in the visible region due to phycobiliproteins (PBPs). The spectral fluorescence signatures by the traditional peak picking method exhibited three peaks, one was protein-like, and the other two were humic-like. Apart from these, Trichodesmium exhibited strong protein-like fluorescence with 370/460 nm (Ex/Em), which is a signature of cyanobacteria. A parallel factor analysis (PARAFAC) on the fluorescence excitation-emission matrix (EEM) of Trichodesmium dataset fitted a 3-component model of which one was protein-like, and two were humic-like. The fluorescence index (FI) values during Trichodesmium bloom was very high (~ 3) compared with the typical range of 1.2-1.8 observed for the natural waters. Bloom degradation experiments proved that increase in tryptophan fluorescence enhances the CDOM absorption. Our study indicates that Trichodesmium blooms provide a rich source of organic matter in the coastal waters and long-term monitoring of these blooms is essential for understanding the health of ecosystem.

Acute undifferentiated fever in India: a multicentre study of aetiology and diagnostic accuracy.

BACKGROUND: The objectives of this study were to determine the proportion of malaria, bacteraemia, scrub typhus, leptospirosis, chikungunya and dengue among hospitalized patients with acute undifferentiated fever in India, and to describe the performance of standard diagnostic methods. METHODS: During April 2011-November 2012, 1564 patients aged ≥5 years with febrile illness for 2-14 days were consecutively included in an observational study at seven community hospitals in six states in India. Malaria microscopy, blood culture, Dengue rapid NS1 antigen and IgM Combo test, Leptospira IgM ELISA, Scrub typhus IgM ELISA and Chikungunya IgM ELISA were routinely performed at the hospitals. Second line testing, Dengue IgM capture ELISA (MAC-ELISA), Scrub typhus immunofluorescence (IFA), Leptospira Microscopic Agglutination Test (MAT), malaria PCR and malaria immunochromatographic rapid diagnostic test (RDT) Parahit Total™ were performed at the coordinating centre. Convalescence samples were not available. Case definitions were as follows: Leptospirosis: Positive ELISA and positive MAT. Scrub typhus: Positive ELISA and positive IFA. Dengue: Positive RDT and/or positive MAC-ELISA. Chikungunya: Positive ELISA. Bacteraemia: Growth in blood culture excluding those defined as contaminants. Malaria: Positive genus-specific PCR. RESULTS: Malaria was diagnosed in 17% (268/1564) and among these 54% had P. falciparum. Dengue was diagnosed in 16% (244/1564). Bacteraemia was found in 8% (124/1564), and among these Salmonella typhi or S. paratyphi constituted 35%. Scrub typhus was diagnosed in 10%, leptospirosis in 7% and chikungunya in 6%. Fulfilling more than one case definition was common, most frequent in chikungunya where 26% (25/98) also had positive dengue test. CONCLUSIONS: Malaria and dengue were the most common causes of fever in this study. A high overlap between case definitions probably reflects high prevalence of prior infections, cross reactivity and subclinical infections, rather than high prevalence of coinfections. Low accuracy of routine diagnostic tests should be taken into consideration when approaching the patient with acute undifferentiated fever in India.

Contrasting patterns in pH variability in the Arabian Sea and Bay of Bengal.

Continuous understanding of the ongoing ocean acidification (OA) is essential for predicting the future impact of OA on marine ecosystems. Here we report the results of open ocean time-series measurements (19 cruises) of seawater pH in total hydrogen ion scale (pHT) and associated parameters in the Arabian Sea (AS) and the Bay of Bengal (BoB). During southwest monsoon (SWM), the pHT within the 30 to 100 m water column shows the maximum difference between the two basins with BoB pHT being lower (up to ~0.39 units) than AS which could be due to freshwater influx from rivers, mixed layer dynamics, and cold-core eddies. However, during Spring inter-monsoon (SIM), the pHT of BoB follows the trend of AS. A contrasting finding is that the lowest pHT occurs at 350 to 500 m in the BoB while it is ~1000 m in the AS. The pHT within the 150 to 1500 m layer of these two basins shows lower values by 0.03 (±0.02) in the BoB as compared to the AS. The possible reasons for the low pHT within the BoB oxygen minimum zone (OMZ) could be due to intrusion of western Pacific water in the BoB, freshwater influx from rivers, variations in OMZ of the two basins, higher temperature (~2°C) within the OMZ of the AS, and denitrification in the AS. The pHT in both the basins (500 to 1000 m) is lower than in the North Atlantic and higher than in the North Pacific waters; however, the pHT in the 200 to 500 m is lower in the BoB than in all these basins. This study highlights the under-saturation of calcium carbonate at very shallow depths (~ 100 m) in the BoB, indicating that the plankton in the BoB are facing a major risk from OA compared to the AS and need further investigation.

Rice husk as a potential source of silicate to oceanic phytoplankton.

Global oceans are witnessing changes in the phytoplankton community composition due to various environmental stressors such as rising temperature, stratification, nutrient limitation, and ocean acidification. The Arabian Sea is undergoing changes in its phytoplankton community composition, especially during winter, with the diatoms being replaced by harmful algal blooms (HABs) of dinoflagellates. Recent studies have already highlighted dissolved silicate (DSi) limitation and change in Silicon (Si)/Nitrogen (N) ratios as the factors responsible for the observed changes in the phytoplankton community in the Arabian Sea. Our investigation also revealed Si/N < 1 in the northern Arabian Sea, indicating DSi limitation, especially during winter. Here, we demonstrate that rice husk with its phytoliths is an important source of bioavailable DSi for oceanic phytoplankton. Our experiment showed that a rice husk can release ∼12 µM of DSi in 15 days and can release DSi for ∼20 days. The DSi availability increased diatom abundance up to ∼9 times. The major benefitted diatom species from DSi enrichment were Nitzshia spp., Striatella spp., Navicula spp., Dactiliosolen spp., and Leptocylindrus spp. The increase in diatom abundance was accompanied by an increase in fucoxanthin and dimethyl sulphide (DMS), an anti-greenhouse gas. Thus, the rice husk with its buoyancy and slow DSi release has the potential to reduce HABs, and increase diatoms and fishery resources in addition to carbon dioxide (CO2) sequestration in DSi-limited oceanic regions such as the Arabian Sea. Rice husk if released at the formation site of the Subantarctic mode water in the Southern Ocean could supply DSi to the thermocline in the global oceans thereby increasing diatom blooms and consequently the biotic carbon sequestration potential of the entire ocean.

Malformation in coccolithophores in low pH waters: evidences from the eastern Arabian Sea.

Oceanic calcifying plankton such as coccolithophores is expected to exhibit sensitivity to climate change stressors such as warming and acidification. Observational studies on coccolithophore communities along with carbonate chemistry provide important perceptions of possible adaptations of these organisms to ocean acidification. However, this phytoplankton group remains one of the least studied in the northern Indian Ocean. In 2017, the biogeochemistry group at the Council for Scientific and Industrial Research-National Institute of Oceanography (CSIR-NIO) initiated a coccolithophore monitoring study in the eastern Arabian Sea (EAS). Here, we document for the first time a detailed spatial and seasonal distribution of coccolithophores and their controlling factors from the EAS, which is a well-known source of CO2 to the atmosphere. To infer the seasonality, data collected at three transects (Goa, Mangalore, and Kochi) during the Southwest Monsoon (SWM) of 2018 was compared with that of the late SWM of 2017. Apart from this, the abundance of coccolithophores was studied at the Candolim Time Series (CaTS) transect, off Goa during the Northeast Monsoon (NEM). The most abundant coccolithophore species found in the study region was Gephyrocapsa oceanica. A high abundance of G. oceanica (1800 × 103cells L-1) was observed at the Mangalore transect during the late SWM despite experiencing low pH and can be linked to nitrogen availability. The high abundance of G. oceanica at Mangalore was associated with high dimethylsulphide (DMS). Particulate inorganic carbon (PIC) and scattering coefficient retrieved from satellites also indicated a high abundance of coccolithophores off Mangalore during the late SWM of 2017. Interestingly, G. oceanica showed malformation during the late SWM in low pH waters. Malformation in coccolithophores could have a far-reaching impact on the settling fluxes of organic matter and also on the emissions of climatically important gases such as DMS and CO2, thus influencing atmospheric chemistry. The satellite data for PIC in the EAS indicates a high abundance of coccolithophore in recent years, especially during the warm El Nino years (2015 and 2018). This warrants the need for a better assessment of the fate of coccolithophores in high-CO2 and warmer oceans.

Clinical features and risk factors for death in acute undifferentiated fever: A prospective observational study in rural community hospitals in six states of India.

BACKGROUND: Acute undifferentiated fever (AUF) ranges from self-limiting illness to life-threatening infections, such as sepsis, malaria, dengue, leptospirosis and rickettsioses. Similar clinical presentation challenges the clinical management. This study describes risk factors for death in patients hospitalized with AUF in India. METHODS: Patients aged ≥5 y admitted with fever for 2-14 d without localizing signs were included in a prospective observational study at seven hospitals in India during 2011-2012. Predictors identified by univariate analysis were analyzed by multivariate logistic regression for survival analysis. RESULTS: Mortality was 2.4% (37/1521) and 46.9% (15/32) died within 2 d. History of heart disease (p=0.013), steroid use (p=0.011), altered consciousness (p<0.0001), bleeding (p<0.0001), oliguria (p=0.020) and breathlessness (p=0.015) were predictors of death, as were reduced Glasgow coma score (p=0.005), low urinary output (p=0.004), abnormal breathing (p=0.006), abdominal tenderness (p=0.023), leucocytosis (p<0.0001) and thrombocytopenia (p=0.001) at admission. Etiology was identified in 48.6% (18/37) of fatal cases. CONCLUSIONS: Bleeding, cerebral dysfunction, respiratory failure and oliguria at admission, suggestive of severe organ failure secondary to systemic infection, were predictors of death. Almost half of the patients who died, died shortly after admission, which, together with organ failure, suggests that delay in hospitalization and, consequently, delayed treatment, contribute to death from AUF.

Impact of physical processes on oxygen loss and production of hydrogen sulphide and methane in a tropical freshwater reservoir.

Carbon neutrality of tropical reservoirs is a big concern in recent years as some estimates project high methane emission from these reservoirs. While there are studies available on the impact of physical processes (stratification and mixing) on the biogeochemistry of tropical reservoirs, not much information is available on the inter-annual variability in the low-oxygen conditions and production/accumulation of hydrogen sulphide (H2S) and methane (CH4) during summer. This paper presents time series data based on monthly in situ observations from a tropical reservoir (Tillari, Maharashtra) situated in the Western Ghats in India. Sampling was carried out for temperature, dissolved oxygen (DO), H2S, and CH4 at a fixed location from March 2010 until June 2014. The reservoir experiences stable stratification during summer (March to June) with complete loss of oxygen and production of H2S (max. ~ 9 µM) and CH4 (max. ~ 185 µM) in the profundal zone. During the summer stratification, the hypolimnion acted as a pool of CH4 with integrated values ranging between 3502 and 41,632 mg m-2. However, the intensity and duration of anoxia varied during different years, influencing H2S and CH4 production. Mixing in the reservoir was observed between July and September in association with the monsoonal runoff, which increased the DO concentrations in the sub-surface layers. Besides, complete mixing was observed between December and February due to winter convection. This, however, was found to play an important role, as weaker mixing in the preceding year was associated with severe oxygen loss in the profundal zone during the following summer with a production of H2S and CH4. In contrast, more robust mixing during winter led to moderate low-oxygen conditions with less production of these gases in the subsequent summer. Based on our observations and considering a large number of reservoirs in the tropics, we hypothesise that with the present trends of global warming and less cold winters, low-oxygen conditions in the profundal zone may become more severe in the future with positive feedback on H2S and CH4 production during summer.

Total organic carbon and its role in oxygen utilization in the eastern Arabian Sea.

We report seasonal and temporal variation of total organic carbon (TOC) in the eastern Arabian Sea (AS). In comparison to the deep, TOC in the top 100 m showed spatial variation with higher concentrations towards northern AS during North east monsoon (NEM) and South west monsoon (SWM). A comparison with the US-JGOFS data (1995) shows warmer temperatures, enhanced TOC and low chlorophyll in the recent years. High TOC is associated with Arabian Sea high saline waters (ASHSW), advected from the Arabian Gulf, might have resulted in an enhancement of TOC in the eastern AS. This excess TOC supports a high abundance of bacteria despite the low primary productivity. TOC oxidation accounted for 14.3% and 22.5% of oxygen consumption for waters with potential density between 24.5 and 27.3 kg/m3. This study attains great significance considering the missing links with respect to the role of transport processes in ocean deoxygenation under ongoing warming scenarios.

Assessment of crustal element and trace metal concentrations in atmospheric particulate matter over a coastal city in the Eastern Arabian Sea.

Major/crustal elements (Al, Ca, Mg, K, and Fe) and trace metals (Mn, Cr, Cu, Pb, Zn, and Ni) in atmospheric particulate matter at three sites in Goa (a coastal city in the Eastern Arabian Sea) were assessed during winter (December) and summer (March-May) months of 2015. A significant spatial and temporal variability was observed in PM10 mass concentration, crustal element, and trace metal composition at the sampling area (pristine, urban, and industrial locations). Using a diagnostic crustal element ratio (Fe/Al, Ca/Al, and Mg/Al), mineral dust components were characterized and found to show large spatial and temporal variability. The concentration levels of trace metals were further assessed for enrichment factor (EF) analysis, wherein reported elements were classified into two major groups. The first group consists of Cr, Cu, and Pb with 10< EF < 100 compared to continental crustal values (w.r.t. Al), suggesting a dominant contribution from anthropogenic sources with minor contribution from natural sources; the second group consists of Zn and Ni showing very high EF (>100)-these are solely derived from anthropogenic sources. Source identification for trace metals was further assessed based on principle component analysis (PCA). PCA highlights that the major contribution of trace metals is from anthropogenic emissions at all three locations. However, contributions from mineral dust were observed at pristine and urban locations during winter months. The reported data of trace metal concentrations in aerosols give baseline information on the atmospheric supply of micronutrients to the Arabian Sea, which has implications for the various surface ocean biogeochemical processes.Implications: This paper reports on crustal and trace metal composition and concentration level in atmospheric aerosols over a coastal city located on the Eastern Arabian Sea. This study highlights the role of various factors (meteorology and emission sources) controlling the abundance of metals over a coastal site. The contribution from various sources (anthropogenic vis-à-vis natural) has also been identified using enrichment factor analysis as well as principle component analysis. This study has implications for the supply of micronutrients to the coastal Arabian Sea, which can significantly impact various surface ocean biogeochemical processes.

Role of oceanic fronts in enhancing phytoplankton biomass in the eastern Arabian Sea during an oligotrophic period.

In the present study, using in-situ and satellite observations, we investigate the influence of physical processes on the enhancement of phytoplankton biomass in the eastern Arabian Sea (EAS). Water column measurements were carried out from 9°N to 21°N (stations II-2 to II-14) along 68°E transect in the EAS during the beginning of fall intermonsoon (FIM) of 2014. Both in-situ and satellite-derived chlorophyll a (Chl a) showed higher biomass at 15°N (station II-8) compared to northern and southern stations. We explored the possible physical processes which can lead to high biological productivity at this station. Our study shows that nearly two times enhancement in Chl a at station II-8 was contributed by an open-ocean front, which occurred two days before the measurement. Based on phytoplankton marker pigments, it was evident that haptophytes were abundant at II-8 with a minor contribution from diatoms and dinoflagellates. This condition also led to a high concentration (4.9 nM) of dimethylsulphide (DMS), an anti-green house gas with a net flux of 3.76 µmol m-2d-1 at this site. Among the picophytoplankton, Synechococcus were abundant at this station, however Prochlorococcus were absent as confirmed by both marker pigment and flow cytometric counts. The case study presented here demonstrates the dynamic nature of open ocean fronts and their overall contribution to the productivity of the eastern Arabian Sea during the oligotrophic inter-monsoon period.

Variability of organic nitrogen and its role in regulating phytoplankton in the eastern Arabian Sea.

The relative importance of organic nitrogen (ON) and inorganic nitrogen (IN) as nitrogen sources for the phytoplankton communities were studied through a seasonal time series of nitrogen species in the eastern Arabian Sea. Seasonal data on nitrogen species showed that ON dominates the system throughout the year and account for >90% of the total nitrogen (TN) during north east monsoon (NEM). The average ON concentration in the study region was 11.5 µM during NEM as compared to 7.2 µM during south west monsoon (SWM). The contribution of picoplankton to the total phytoplankton pool (fpico) increased from 19% during SWM to 36% in NEM. Along with cyanobacteria, the diatoms Thalassionema nitzchoides and Thalassiosira sp. and among the dinoflagellates Pronoctiluca sp. were found to proliferate in ON rich waters. We suggest atmospheric deposition, riverine-input, resuspension of bottom sediment and ground water intrusion as the possible sources of ON in the study region.

Methane stimulates massive nitrogen loss from freshwater reservoirs in India.

The fate of the enormous amount of reactive nitrogen released to the environment by human activities in India is unknown. Here we show occurrence of seasonal stratification and generally low concentrations of dissolved inorganic combined nitrogen, and high molecular nitrogen (N2) to argon ratio, thus suggesting seasonal loss to N2 in anoxic hypolimnia of several dam-reservoirs. However, 15N-experiments yielded low rates of denitrification, anaerobic ammonium oxidation and dissimilatory nitrate reduction to ammonium-except in the presence of methane (CH4) that caused ~12-fold increase in denitrification. While nitrite-dependent anaerobic methanotrophs belonging to the NC10 phylum were present, previously considered aerobic methanotrophs were far more abundant (up to 13.9%) in anoxic hypolimnion. Methane accumulation in anoxic freshwater systems seems to facilitate rapid loss of reactive nitrogen, with generally low production of nitrous oxide (N2O), through widespread coupling between methanotrophy and denitrification, potentially mitigating eutrophication and emissions of CH4 and N2O to the atmosphere.

Serovar prevalence of Leptospira in semirural India and the development of an IgM-based indirect ELISA.

INTRODUCTION: Leptospirosis is a major public health problem in India. However, it has been underreported and under-diagnosed due to a lack of awareness of the disease, a functional surveillance system, and appropriate laboratory diagnostic facilities. METHODOLOGY: This multicenter study aimed to understand the Leptospira serovars causing leptospirosis in seven secondary-level hospitals in six states in India. Since early and accurate diagnosis of leptospirosis is one of the challenges faced by clinicians in India due to the poor specificity and sensitivity of commercially available diagnostic systems, an in-house indirect enzyme-linked immunosorbent assay (ELISA) was developed. Genomic DNA from L. interrogans serovar Canicola was used for polymerase chain reaction amplification, cloning, and expression of the lipL32 gene in E. coli to amplify, clone, and express the lipL32 gene. RESULTS: Australis was the common serovar seen at all the study centers. Serovar Icterohaemorrhagiae was seen in samples from Tamil Nadu and Assam. In-house ELISA was standardized using the purified recombinant LipL32 polypeptide and was used to evaluate serum. Subsequently, acute serum samples from leptospirosis patients (n = 60) were screened. Compared to the gold standard, the microscopic agglutination test, sensitivity and specificity of the in-house ELISA was 95% and 90%, respectively. CONCLUSIONS: Understanding Leptospira serovars circulating in leptospirosis-endemic areas will help to formulate better vaccines. LipL32-based ELISA may serve as a valuable tool for early diagnosis of leptospirosis.