Ultrastructure of Endothelial Cells of Myocardial Capillaries in Burn Septicotoxemia.

We studied ultrastructure and vesicular structures in endothelial cells of myocardial micro-vessels in burn patients. Electron microscopy revealed a significant decrease in volume density of vesicular structures in the endotheliocytes of myocardial capillaries in patients with burn septicotoxemia. The observed structural signs of endothelial dysfunction revealed in this category of patients can be a promising area for further research and for the development of methods of pathogenetic correction of myocardial disorders in the case of burn injury.

An excess of cosmic ray electrons at energies of 300-800 GeV.

Galactic cosmic rays consist of protons, electrons and ions, most of which are believed to be accelerated to relativistic speeds in supernova remnants. All components of the cosmic rays show an intensity that decreases as a power law with increasing energy (for example as E(-2.7)). Electrons in particular lose energy rapidly through synchrotron and inverse Compton processes, resulting in a relatively short lifetime (about 10(5) years) and a rapidly falling intensity, which raises the possibility of seeing the contribution from individual nearby sources (less than one kiloparsec away). Here we report an excess of galactic cosmic-ray electrons at energies of approximately 300-800 GeV, which indicates a nearby source of energetic electrons. Such a source could be an unseen astrophysical object (such as a pulsar or micro-quasar) that accelerates electrons to those energies, or the electrons could arise from the annihilation of dark matter particles (such as a Kaluza-Klein particle with a mass of about 620 GeV).

[Classification of amino acids based on a comparative analysis of contacts in DNA-protein complexes and specific DNA-protein interactions].

The classification of amino acid residues based on the events of contact formation between distinct amino acid and selected nucleotides was constructed. Thus, the most integral properties, that characterize interactions in organization of DNA-protein complexes, were used. We applied the Voronoi-Delaunay tessellation to draw statistics of contacts and area of contacts for the set included 1937 DNA-protein complexes. Similarities of amino acid residues have been searched for based on the comparison of corresponded rows and matrixes of contacts and areas of contacts. Nine measures of distance were used for estimation of rows similarity degree. The procedure of clustering amino acids in groups included three hierarchical and two nonhierarchical methods. A total tree was built using nine techniques of estimating distance with three hierarchical clustering methods. It was shown that clustering centers in the main groups are always constant while other relationships between objects vary. Clustering of binary associations was found for the most amino acids. Major classes of up to six amino acids correspond to the certain local structures of the polypeptide chain in the context of amino acid composition. These data should be taken into account when designing DNA-protein ligands.

[Mebeverina drugs in the treatment of functional disorders of the digestive system].

Despite the identical content of mebeverine hydrochloride, Duspatalin and Niaspam, produced by different technological processes, can't be considered interchangeable as they are known to have different clinical effect in case of various functional diseases of alimentary organs. Duspatalin is effective in relieving biliary disfunction symptoms, Oddy's sphincter hypertonicity in particular, but has little effect on intestinal dyskinesia. Niaspam is known to have little effect on biliary dyskinesia, but has a prominent positive clinical effect on intestinal dyskinesia.

[The organic carbon--issues of hygienic regulation and harmonization].

This study is devoted to the investigation of possibility to use the total organic carbon as regulated index in drinking water as well as to the issues of hygienic regulation and harmonizing this index with the standards of other countries. Basing on the results of 3 years lasting investigation carried out by Municipal Unitary Enterprise "Vodokanal" of Yekaterinburg city permits to propose as the most informative and reliable index of the presence of organic substances in drinking water the content of total organic carbon in comparison with currently regulated permanganate oxidability, chemical and biochemical oxygen consumption.

Fully Coupled Three-Dimensional Simulation of Downward Flame Spread over Combustible Material.

Three-dimensional simulations of laminar flame propagating downwards the vertical surface of a rigid polyurethane slab heated by a radiative panel are presented and compared with the measurement data. The gas-phase model (ANSYS Fluent) allows for finite-rate volatile oxidation, soot formation and oxidation, emission, transfer, and absorption of thermal radiation. The solid-phase model Pyropolis considers heat transfer across the material layer and generation of combustible volatiles in thermal decomposition of the material. Kinetic model of material decomposition is derived to obey the microscale combustion calorimetry data for different heating rates. Transient behavior of propagating flame and pyrolysis zone, as well as spatial distributions of heat flux components, temperature, and mass burning rates over the specimen surface are examined. Variation of the thermal properties of the material during its thermal decomposition, as well as the specimen surface emissivity and reradiation are shown to be the important issues strongly affecting model predictions. Two distinct modes of counterflow flame spread, thermal and kinetic, are identified. In the thermal mode corresponding to fast chemistry in the gaseous flame, the flame propagation velocity is governed by the heating rate of the combustible material ahead of the flame front. Alternatively, in the kinetic mode, it is limited by the burning velocity of the volatile-air mixture forming ahead of the flame front. Simulation results are favorably compared with the measured propagation velocity.

[Transcranial magnetic stimulation in the treatment of depressive disorder in Parkinson's disease]. / Primenenie transkranial'noi magnitnoi stimulyatsii v lechenii depressivnogo rasstroistva pri bolezni Parkinsona.

The prevalence of affective disorders in patients with Parkinson's disease (PD) is significantly higher than in the general population of people of the same age. It is known that the effectiveness of antidepressants in PD with depressive disorder is lower than in patients with primary depression. For depression resistant to standard treatments, other therapeutic strategies are available. Transcranial magnetic stimulation (TMS) is one of the most popular and safe brain stimulation methods that has been successfully used to treat primary depression. The accumulation of experience in the use of TMS has made it possible to expand the application of the method to other diseases, including PD. The article provides literature data on the use of different TMS protocols for the treatment of depressive disorder in patients with PD.

A Review of the EUSO-Balloon Pathfinder for the JEM-EUSO Program.

EUSO-Balloon is a pathfinder for JEM-EUSO, the mission concept of a spaceborne observatory which is designed to observe Ultra-High Energy Cosmic Ray (UHECR)-induced Extensive Air Showers (EAS) by detecting their UltraViolet (UV) light tracks "from above." On August 25, 2014, EUSO-Balloon was launched from Timmins Stratospheric Balloon Base (Ontario, Canada) by the balloon division of the French Space Agency CNES. After reaching a floating altitude of 38 km, EUSO-Balloon imaged the UV light in the wavelength range ∼290-500 nm for more than 5 hours using the key technologies of JEM-EUSO. The flight allowed a good understanding of the performance of the detector to be developed, giving insights into possible improvements to be applied to future missions. A detailed measurement of the photoelectron counts in different atmospheric and ground conditions was achieved. By means of the simulation of the instrument response and by assuming atmospheric models, the absolute intensity of diffuse light was estimated. The instrument detected hundreds of laser tracks with similar characteristics to EASs shot by a helicopter flying underneath. These are the first recorded laser tracks measured from a fluorescence detector looking down on the atmosphere. The reconstruction of the direction of the laser tracks was performed. In this work, a review of the main results obtained by EUSO-Balloon is presented as well as implications for future space-based observations of UHECRs.

[Peculiarities of affective disorders in residual cerebral insufficiency]. / Osobennosti affektivnykh rasstroistv pri tserebral'noi rezidual'no-organicheskoi nedostatochnosti.

AIM: To determine clinical and psychopathological features of anxiety-depressive disorders in patients with cerebral residual organic insufficiency. MATERIAL AND METHODS: One hundred and fourteen patients with anxiety-depressive disorders and cerebral residual-organic insufficiency were studied. Psychopathological, psychometric, statistical methods were used. RESULTS AND CONCLUSION: Asthenia was identified as a core syndrome in anxiety-depressive disorders developed in patients with cerebral residual-organic insufficiency. Severe anxiety and depression with somatization of symptoms were characteristic of the structure of affective pathology. The somatization of affect, interpersonal sensitivity, marked asthenic and autonomic disorders as well as negative attitudes about their own condition, abilities and activity were main features of affective disorders in patients with cerebral residual-organic insufficiency. Clinical characteristics of cerebral residual-organic insufficiency and the diversity of its manifestations, including the combination with mental disorders, determine the relevance of further research.

[Geometrical analysis of protein-DNA interactions on the basis of the Voronoi-Delaune tessellation].

A new method for the rigorous analysis of DNA-protein contacts has been developed on the basis of Voronoi tessellation. This method permits one to determine close neighbors on the atomic level and compute the area of contact by edges of Voronoi polyhedra. Based on the results of the study of 1109 protein-DNA complexes from PDB, it was demonstrated that about one third of the contacts are the contacts with positively charged Arg and Lys. There is distinct amino acid prevalence for nucleotides: for A - Pro; for T - His; for G - Asp; for C - Trp, Asp, Glu. Therefore, GC pairs prefer to interact with negatively charged residues, and alanine and methionine, whereas AT pairs prefer to interact with histidine and unpolar residues.

CMOS-compatible biosensor for L-carnitine detection.

A CMOS-compatible ISFET with a Ta2O5 sensitive surface was developed. The structure was optimized for achieving high sensitivity using a subthreshold operation mode and by reducing the influence of the capacitances on the value of subthreshold swing. The developed ISFET was used as a basis for a biosensor for L-carnitine detection. To this end, carnitine acetyltransferase was immobilized on the ISFET sensitive surface. The immobilized enzyme was active (0.082 U/g model plate). The complete microsystem, consisting of a packaged chip, an immobilized enzyme and a microfluidic channel, detected L-carnitine at a range of 0.2-100 µM with a LOD of 0.2 µM. The biosensor response was linear in the range of 0.2-50 µM of L-carnitine with sensitivity 18.0 ±â€¯1.7 mV/µM. An experiment with artificial urine containing 1.3 µM L-carnitine showed that the proposed biosensor could be used on a real sample. Therefore, a new sensor specially optimized for biosensing CMOS-compatible ISFET structures and direct determination of L-carnitine with immobilized carnitine acetyltransferase was developed.

Recovery of acute renal failure following bilateral renal artery angioplasty and stenting.

Atherosclerotic renovascular disease commonly coexists with chronic kidney disease, and its optimal management remains unsettled. In this case report, we describe a 75-year-old woman with chronic kidney disease and critical atherosclerotic bilateral renal artery stenosis, who presented with a hypertensive emergency and developed acute renal failure following antihypertensive treatment. Bilateral percutaneous transluminal renal angioplasties (PTRA) with stent placement were performed and resulted in immediate recovery of renal function. The existing literature on this impressive response to PTRA is reviewed and discussed.

[Treatment of patients with essential hypertension using bifunctional 24-hour blood pressure and ECG monitoring].

The results of 24-hour blood pressure monitoring (BPM) help to divide patients into therapeutic groups according to the leading hemodynamic mechanism offorming essential arterial hypertension (EAH). In patients with a mean day heart rate of > or =73 bpm antihypertensive therapy should begin with beta1-adrenoblockers when not contraindicated. In patients with a mean day heart rate of < or =73 bpm antihypertensive therapy may be started with preparations of other pharmaceutical groups. In this study, Plendil or Concor was administered as a chronotherapy; later their combination was used, and diuretics or ACE inhibitors were added when necessary. At any stage of the study, starting from the second week of therapy, if blood pressure (BP) was normal and there were ischemic episodes according to matched 24-hour BPM and ECG, Cardiket and, when not contraindicated, Aspirin, were added. The offered algorithm of choice of therapy made it possible to achieve a good antihypertensive effect in 84% of patients and a satisfactory effect in 7% of patients, which was accompanied by a tendency towards the shortening of total ischemia duration and lowering the frequency of myocardial hypokinesia. The effect of the therapy was poor only in 9% of patients, 2% of whom one left the study due to adverse reactions before BP was normalized. BP was normalized on the 8th week of treatment in 75% of patients. It is appropriate to include matched 24-hour BPM and ECG in the follow-up of patients with EAH and coronary artery disease upon discharge under the conditions of routine physical load. According to 24-hour BPM, mean day heart rate and total length of ischemia grow during this period due to an increase in physical and emotional load, which requires correction of the therapy.

[Detection of tuberculosis in children and adolescents in the Primorye Territory].

The epidemiological indices and the methods for detecting tuberculosis have been analyzed in the children and adolescents of the Primorye Territory. It has been established that morbidity among children became stable by 2003 and was 23.6 per 100,000; in adolescents it remains high - 79.5 per 100,000 in 2003. The association of the role of different detection methods with the age group is now a specific feature of tuberculosis in children and adolescents. Mass tuberculin diagnosis plays a decisive role in the detection of tuberculosis only in children aged 5-7 years. In other age groups, tuberculosis is frequently identified at contacts with a physician. Radiation is the leading technique in detecting tuberculosis in adolescents. The consideration of the specific features of tuberculosis detection in relation to the age group assisted one in the selecting the most optimum strategy of tuberculosis control in children and adolescents.

Wave collapse in plasmas and fluids.

This lecture is a review of recent results (obtained mainly by the author and his co-authors) in the wave collapse theory with applications to plasma physics, fluid dynamics and nonlinear optics as well. The main attention in the review is paid to the qualitative reasons of the wave collapse and to the exact methods based on the integral estimations. Both approaches are applied to both the nonlinear Schrodinger equation and the two-dimensional generalized Benjamin-Ono equation which describes self-focusing of low-frequency oscillations in the boundary layer. (c) 1996 American Institute of Physics.

Growth and enzymological characteristics of a pink-pigmented facultative methylotroph Methylobacterium sp. MB1.

Growth characteristics of batch and continuous cultures of the pink facultative methylotroph Methylobacterium sp. MB1 were determined. The response of a chemostat culture to a pulse increase of methanol concentration was studied. Malate, succinate and oxaloacetate additions to the methanol-supplemented medium decreased batch culture growth inhibition by methanol. The carotenoid content in cells grown in a chemostat decreased with increasing growth rate. The key enzyme activities of C1-metabolism were measured in a chemostat culture at different dilution rates.

[Statistical criteria for evaluating the spatial structure, used in the reverse case of forming protein globules]. / Statisticheskie usloviia primenimosti opisanii prostranstvennoi struktury, ispol'zuemykh v obratnoi zadache formirovaniia belkovoi globuly.

Statistical requirements to spatial structure representation used in the inverse folding problem have been considered. The representation by the distance from centers of side chaines to center of backbone of the molecule has been elaborated. It allows to select residue position and environmental properties and thus to improve computer methods for estimation 3D-1D compatibility.

[New criteria in the inverse problem of protein structure formation]. / Novye kriterii v obratnoi zadache strukturoobrazovaniia belka.

New approach to the inverse protein folding problem has been developed. It is helpful for search amino acid sequences of proteins having certain tertiary structure. Also it looks for the best three dimensional structure among known ones for a given amino acid sequence. We introduced new parameters for delineating geometry and physical properties of protein tertiary structure and new evaluation function to distinguish between correct fold and large number of misfolds. The method can be used for checking de novo constructed proteins and for creating enlarged bank of three dimensional protein structures on the basis of known primary protein structures.

[Experimental development of immunity to pertussis]. / Osobennosti formirovaniia protivokokliushnogo immuniteta v éksperimente.

Experiments on animals inoculated intracerebrally with B. pertussis culture No 18-323, serovar 1.2.3, revealed that among sera against B. pertussis strains of different serovars sera against the strains of serovar 1 showed the highest protective potency, and sera against the strains of serovar 1.2 showed the lowest protective potency. In active immunization experiments vaccines from the strains of serovar 1 proved to be least effective, while the immunogenic properties of vaccines from the strains of serovar 1.2 were found to be most pronounced. Since this discrepancy in the above results is due to the fact that the strains of serovar 1 in small doses were unable to create a stable focus of antigenic stimulation, it is not expedient to include strains belonging to serovars other than 1.2 into vaccinal preparations.

[Creation of the library of Bordetella pertussis genome clones and detection of clones containing genes for the synthesis of antigenic determinants of the whooping cough microbe]. / Sozdanie klonoteki genoma Bordetella pertussis i obnaruzhenie v nei klonov, soderzhashchikh geny sinteza antigennykh determinant kokliushnogo mikroba.

Escherichia coli clones containing hybrid phasmids with the inserts of B. pertussis DNA were obtained with the use of a phasmid vector. The total amount of the clones thus obtained was 97,000, which considerably exceeded the volume of the clone library necessary for the detection of individual genes with probability approximating 1. The hybrid plasmids were shown to contain 6-19 kilobases. The screening of the clone library was carried out by means of the enzyme immunoassay (EIA). The assay was aimed at detecting clones containing the genes of the subunits of B. pertussis lymphocytosis-stimulating factor (LSF). The EIA techniques used in this investigation were based on the capacity of LSF for binding with fetuin. Six clones giving positive response were detected. These data suggest the presence and expression of the genes controlling the synthesis of the antigenic determinants of LSF in E. coli cells.