RESUMO
To verify if the peptide preparation <
Assuntos
Lesões Encefálicas , Hipóxia Encefálica , Peptídeos/farmacologia , Animais , Animais Recém-Nascidos , Lesões Encefálicas/tratamento farmacológico , Lesões Encefálicas/metabolismo , Lesões Encefálicas/patologia , Lesões Encefálicas/fisiopatologia , Modelos Animais de Doenças , Humanos , Hipóxia Encefálica/tratamento farmacológico , Hipóxia Encefálica/metabolismo , Hipóxia Encefálica/patologia , Hipóxia Encefálica/fisiopatologia , Peptídeos e Proteínas de Sinalização Intercelular , MasculinoRESUMO
AIM: Abnormal sperm DNA integrity is an important risk factor for male infertility. The aim of this work was to examine sperm DNA fragmentation in a cohort of young male volunteers (n=111; age 21.0+/-0.2 years) from the general population and establish the association between the level of sperm DNA fragmentation and sperm functional parameters. MATERIALS AND METHODS: Sperm DNA fragmentation index (DFI) was determined by SCSA (sperm chromatin structure assay) using flow cytometry. Standard semen parameters (concentration, motility, and morphology) were evaluated according to the WHO guidelines (2010). RESULTS: and conclusions. In the study cohort, 79.0%, 12.4% and 8.6% of men had normal (DFI<15%), borderline (15 less or equal DFI<27%) and high (DFI more or equal 27%) levels of fragmentation, respectively. Men with impaired spermatogenesis had greater IDF values (14.53+/-1.43%) than men with normal semen parameters (8.88+/-0.77%, p<0.05). There was a statistically significant negative correlation between IFD and ejaculate concentration (r=-0.21, p<0.05), fractions of mobile (r=-0.41, p<0.05) and morphologically normal sperm (r=- 0.34, p<0.05). Testing sperm DNA fragmentation using SCSA technique can be employed in epidemiological studies of male fertility.
Assuntos
Fragmentação do DNA , Infertilidade Masculina/diagnóstico , Sêmen/química , Espermatozoides/ultraestrutura , Adolescente , Adulto , Cromatina/química , Citometria de Fluxo/métodos , Humanos , Masculino , Contagem de Espermatozoides , Espermatogênese , Adulto JovemRESUMO
AIM: To compare treatment of intestinal malrotation in newborns and infants using laparoscopy and laparotomy. MATERIAL AND METHODS: For the period from January 2004 to December 2013 34 Ladd's procedures were performed. Children were divided into 2 groups by 17 patients: laparoscopic (group I) and open treatment (group II). RESULTS: Both groups had similar demographic and other preoperative parameters. There were significant differences in duration of operation between both groups (61 vs. 70 minutes). Nutrition was initiated earlier after mini-invasive treatment (1.5 days vs. 3 days) and the time need for full enteral nutrition was also less (4.2 days vs. 6.9 days). Hospital-stay was shorter in group I (7.7 vs. 10.2 days). Number of early postoperative complications was similar in groups. Incidence of remote complications was higher in laparotomy group but the differences were not significant. CONCLUSION: Our results showed that endoscopic correction of congenital anomalies of intestinal rotation provides better postoperative results than open surgery and can be widely used in young children.
Assuntos
Anormalidades do Sistema Digestório/cirurgia , Procedimentos Cirúrgicos do Sistema Digestório , Volvo Intestinal/cirurgia , Laparoscopia , Laparotomia , Complicações Pós-Operatórias/prevenção & controle , Sepse/prevenção & controle , Anatomia Comparada , Anormalidades do Sistema Digestório/diagnóstico , Procedimentos Cirúrgicos do Sistema Digestório/efeitos adversos , Procedimentos Cirúrgicos do Sistema Digestório/métodos , Feminino , Humanos , Lactente , Recém-Nascido , Volvo Intestinal/diagnóstico , Laparoscopia/efeitos adversos , Laparoscopia/métodos , Laparotomia/efeitos adversos , Laparotomia/métodos , Masculino , Complicações Pós-Operatórias/diagnóstico , Complicações Pós-Operatórias/etiologia , Recidiva , Sepse/diagnóstico , Resultado do TratamentoRESUMO
Earlier we have shown that in newborn rats the glucose level in blood serum changes depending on the pattern of spontaneous periodic motor activity (SPMA). During rest periods, both under dominant decasecond rhythm and under dominant minute rhythm, the glucose level in blood serum is higher than during activity periods. We have also shown that the glucose level in the blood serum rises with age reaching the level of adult animals by day 10. In the present study carried out on 3-10-day-old rats under conditions of free behavior the blood glucose content was investigated in different periods of <
Assuntos
Glicemia/metabolismo , Atividade Motora , Estações do Ano , Animais , Animais Recém-Nascidos , RatosRESUMO
For the period January 2002 to December 2013 it was performed 84 interventions for introduction of gastrostomy tube. The first group included 24 open operations and the second group had 60 laparoscopic operations by using of button devices MIC-KEY (Kimberly-Clark, Roswell, USA) in neonates and infants. Statistically significant difference was not observed during comparison of demographic data of patients. Differences in groups were found in statistical analysis of intra- and postoperative parameters (p<0.05). Mean duration of surgery in the first group was 37.29 min, in the second group - 23.97 min. Time to start of feeding and transition to complete enteral nutrition was less in patients who underwent laparoscopic surgery than after open intervention (10.5 and 19.13 hours, 23.79 and 35.88 hours respectively; p<0.05). It was revealed augmentation of hospital stay in the 1st group in comparison with the 2(nd) group (11.71 and 7.09 days respectively; p<0.05). Frequency of postoperative complications was 18.33% in the 2(nd) group and 24% - in the 1st group (p<0.05). The authors consider that button devices are simply and effective technique of gastrostomy establishment in children. It is associated with minimal surgery duration and allows to start early enteral nutrition in comparison with open techniques.
Assuntos
Nutrição Enteral/métodos , Gastrostomia , Laparoscopia , Laparotomia , Complicações Pós-Operatórias , Pesquisa Comparativa da Efetividade , Feminino , Gastrostomia/efeitos adversos , Gastrostomia/métodos , Humanos , Recém-Nascido , Doenças do Recém-Nascido/terapia , Laparoscopia/efeitos adversos , Laparoscopia/métodos , Laparotomia/efeitos adversos , Laparotomia/métodos , Masculino , Procedimentos Cirúrgicos Minimamente Invasivos , Complicações Pós-Operatórias/etiologia , Complicações Pós-Operatórias/prevenção & controle , Estudos Retrospectivos , Federação Russa , Fatores de Tempo , Resultado do TratamentoRESUMO
The study is carried out the 1st-, 3rd-, 5th-, 7th, and 10th-day old free moving or loosely fixed rat pups (P1-10). In satiated and submitted to the 24-h starvation rat pups, parameters of spontaneous periodical motor activity (SPMA) and the blood glucose content were studied. The total glucose level in hungry rat pups was, on average, 1.5-2.5 times lower than in satiated animals. Administration of glucose to hungry rat pups increased 6-11 times its concentration in blood as compared with the animals not obtaining glucose. The glucose administration to the satiated rat pups led to a rise of its level in blood from 2 to 5 times as compared with intact animals, which was 2-3 times less than in the case of hungry animals. Analysis of pattern of motor activity recorded under conditions of the glucose deficit caused by the 24-h starvation of rat pups did not reveal significant changes of ratio of rhythmical components. Introduction of glucose to hungry and to the lesser degree to satiated rat pups led to potentiation of the minute rhythm of activity in all age groups. An exception was the first day after birth when the glucose administration to satiated rat pups promoted an enhancement of the decasecond rhythm and a decrease of the total level of motor activity. Comparison of ontogenetic dynamics of the SPMA parameters and the glucose content in blood of hungry rat pups revealed the clearly expressed regularity absent in the satiated animals: the glucose level in blood was higher during activity than in the state of rest. The performed study has shown that intensity, duration, and, to a degree, pattern of SPMA in the newborn rat pups depend on the level of satiety, and can be significantly changed in the artificially produced hypo- or hyperglycemia. The existing ontogenetic fluctuations in the character of reaction at performance of the glucose tolerance test can be connected both with morphofunctional maturation of the motor system and with immaturity of various chains of carbohydrate metabolism.
Assuntos
Glicemia/metabolismo , Jejum/fisiologia , Intolerância à Glucose/fisiopatologia , Movimento , Fatores Etários , Animais , Animais Recém-Nascidos , Jejum/sangue , Intolerância à Glucose/sangue , Ratos , SaciaçãoRESUMO
BACKGROUND: Thoracoscopic clipping of the patent ductus arteriosus is an alternative to conventional surgical closure via thoracotomy in low birth weight infants. The aim of this study is to compare of these two groups of patients for the last 11 years. METHODS: We reported the data of 127 small children's who underwent standard transaxillary thoracotomy (101 patients - Group I) and video-assisted thoracoscopic surgery for patent ductus arteriosus clipping (26 patients - Group II). The two groups were compared for patients demographics, operative report and postoperative parameters. RESULTS: The groups were similar in terms of demographics and preoperative parameters. There was significant difference in mean operative time between open and thoracoscopic procedure (44.65 min vs 38.46 min; p<0.05). Duration of care in neonatal intensive unit and length of hospital stay were significantly shorter in the Group II (16.44 d vs 8.77 d; p<0.05 and 40.13 d vs 33.65 d; p<0.05). Early complication rates were equivalent between groups (6.93% vs 3.85%; p>0.05). Rate of long-term complications was dominated in the thoracotomy group (19.80% vs 0%; p=0127). CONCLUSION: Thoracoscopic ligation of the patent ductus arteriosus in infants less than 2500 g gave results better than open surgery.
Assuntos
Procedimentos Cirúrgicos Cardíacos/métodos , Permeabilidade do Canal Arterial/cirurgia , Recém-Nascido de Baixo Peso , Cirurgia Torácica Vídeoassistida/métodos , Toracotomia/métodos , Feminino , Seguimentos , Humanos , Recém-Nascido , Masculino , Estudos Retrospectivos , Resultado do TratamentoRESUMO
119 specimens of blood sera collected from measles cases with different vaccination history (aged 4 months to 48 years) on 5th-6th days after rash onset were Investigated using EIA. The obtained results showed that the primary immune response (PIR) was developed in 59 patients; the secondary immune response (SIR) was developed in 60 patients with a significant increase in the specific high avidity IgG (22.34 IU/ml +/- 3.2). The specific IgM were detected in 100% cases studied with capture ELISA in both previously vaccinated and unvaccinated individuals of different age. The specific IgM were detected by indirect ELISA in 100% cases in unvaccinated patients, while IgM positive sera was defined only in 23.3% of individuals with SIR. It was concluded that measles virus infection in previously vaccinated and unvaccinated adults had clinical differences. The role of patients with SIR in virus transmission was discussed.
Assuntos
Imunoglobulina G , Imunoglobulina M , Vacina contra Sarampo , Vírus do Sarampo , Sarampo , Adolescente , Adulto , Criança , Pré-Escolar , Ensaio de Imunoadsorção Enzimática , Humanos , Imunoglobulina G/sangue , Imunoglobulina G/imunologia , Imunoglobulina M/sangue , Imunoglobulina M/imunologia , Lactente , Sarampo/sangue , Sarampo/imunologia , Vacina contra Sarampo/imunologia , Vírus do Sarampo/imunologia , Vírus do Sarampo/isolamento & purificação , Vírus do Sarampo/patogenicidade , Pessoa de Meia-Idade , VacinaçãoRESUMO
Earlier we have shown that administration to newborn rats of the pentose phosphate cycle inhibitor hydroquinone leads to a change in intensity and pattern of spontaneous periodic motor activity (SPMA) characteristic of early stages of development. The most typical was the disappearance of the rest period from the near-minute cycle "activity--rest" and the appearance of uninterrupted motor activity. In several cases, especially after 10 days of development, there was noted an enhancement in the SMPA pattern of the motor activity complexes following in the decasecond rhythm. In this study, on the 3-10-day old rats maintained under conditions of free behavior there was studied the blood glucose content in the animals at various periods of the activity-rest cycle. Apart from the SPMA phase, its composition (pattern) characterizing the maturity level and functional state of spinal motor centers was taken into account. In the 3, 7 and 10-day old rats at the rest period, the glucose concentration was established to differ depending on the motor activity pattern. In the case of the decasecond periodicity, it amounts to 5.7 +/- 0.2, 6.3 +/- +/- 0.3, and 7.7 +/- 0.3 mmol/l, while at the minute one--6.1 +/- 0.4, 7.8 +/- 0.3, and 7.8 +/- 0.1 mmol/l. At the moment of bursts of motor excitation, the glucose concentration falls to 5.2 +/- 0.1, 6.1 +/- 0.4, and 7.1 +/- +/- 0.3 mm at the decasecond and to 5.4 +/- 0.5, 6.7 +/- 0.2, and 7.6 +/- 0.3 mmol/l at the near-minute rhythm (for the 3, 7 and 10-day old animals, respectively). The results obtained on the 5-day rat pups differ qualitatively from those observed in other age groups. Thus, the glucose concentration at the rest period amounts to 6.8 +/- 0.2 at the decasecond and to 6.7 +/- 0.4 mmol/l at the nearminute periodicity. At the period of motor excitation accompanied by the presence of the decasecond activity rhythm, the glucose concentration falls to the level of 6.0 +/- 0.2 mmol/l by differing statistically significantly from the observed one in the rest state. In the case of the appearance of the minute rhythm, the glucose concentration amounts to 5.8 +/- +/- 0.3 mmol/l. The obtained data indicate that reproduction of the minute and decasecond rhythms recorded in composition of SPMA is accompanied by a change in the blood serum glucose level. The degree of a decrease of its concentration correlates with a certain activity rhythm: at the decasecond one the fall is 9, 13, 3, and 7 %, whereas at the minute rhythm--11, 13, 14, and 2% (for the 3-, 5-, 7- and 10-day old rats, respectively). In id to be noted that a certain effect on the activity pattern is produced by the degree of satiety of the rat pups, the gastric emptying being accompanied by an increase in expression of the decasecond rhythm and of brief jerks. Besides, there occur the significant seasonal oscillations of the blood serum glucose concentration in the newborn rat pups--at the summer period it is statistically significantly higher than at the winter-spring period.
Assuntos
Envelhecimento/fisiologia , Glicemia/análise , Atividade Motora/fisiologia , Envelhecimento/sangue , Animais , Animais Recém-Nascidos , Eletromiografia , Músculo Esquelético/fisiologia , Ratos , Restrição FísicaAssuntos
Gastrosquise , Hérnia Umbilical , Parede Abdominal/anormalidades , Parede Abdominal/cirurgia , Gerenciamento Clínico , Gastrosquise/diagnóstico , Gastrosquise/fisiopatologia , Gastrosquise/terapia , Hérnia Umbilical/diagnóstico , Hérnia Umbilical/fisiopatologia , Hérnia Umbilical/terapia , Humanos , Recém-Nascido , Diagnóstico Pré-Natal/métodosRESUMO
The purpose of this study was to investigate the long-term reactive changes in the subventricular zone (SVZ) of the rat brain lateral ventricles after the intraventricular injection of beta-amyloid peptide (BAP). It was found that 3 months after BAP injection ependymal cells and SVZ cells reacted to the intraventricular BAP injection by the formation of glial structures, similar to amyloid plaques in their composition, cell structure and possible developmental mechanism. Using immunohistochemical methods, it was shown that they consisted mainly of reactive astrocytes and microgliocytes. It is suggested that atypical morphogenesis of these structures is associated with the interrelationship between the degenerative and regenerative processes, which involve both ependymal cells and forebrain proliferative zone progenitor cells.
Assuntos
Doença de Alzheimer , Astrócitos/patologia , Microglia/patologia , Prosencéfalo , Doença de Alzheimer/induzido quimicamente , Doença de Alzheimer/patologia , Doença de Alzheimer/fisiopatologia , Peptídeos beta-Amiloides/farmacologia , Animais , Modelos Animais de Doenças , Masculino , Prosencéfalo/patologia , Prosencéfalo/fisiopatologia , Ratos , Ratos WistarRESUMO
Influence of the chronic administration of ropren (Trademark)--a plant preparation based on the neutral fraction of extract of spruce and pine needles--on affective status in male rats with amyloid peptide (Abeta(25-35)) induced amnesia has been studied. Ropren was administered per os in a dose of 8.6 mg/kg for 28 days. Anxiety, depressive-like behavior, and behavioral reactions were assessed in the elevated plus maze, forced swimming, and open field tests. The four-week treatment revealed significant anxiolytic and antidepressant effects of the drug. Ropren administration improved various behavioral parameters. The obtained results show that ropren ameliorates behavioral deficits in animal model relevant to Alzheimer's disease and indicate that Ropren is potentially active in the management of affective impairments in the experimental model under consideration. It also has a profound beneficial effect on the anxiety and depressive-like behavior in rats with model Alzheimer's type dementia, and thus may prove to be a novel natural treatment.
Assuntos
Doença de Alzheimer/tratamento farmacológico , Ansiedade/tratamento farmacológico , Comportamento Animal/efeitos dos fármacos , Depressão/tratamento farmacológico , Terpenos/uso terapêutico , Doença de Alzheimer/complicações , Doença de Alzheimer/fisiopatologia , Animais , Ansiedade/complicações , Ansiedade/fisiopatologia , Depressão/complicações , Depressão/fisiopatologia , Modelos Animais de Doenças , Masculino , Picea/química , Ratos , Ratos Wistar , Terpenos/química , Terpenos/isolamento & purificaçãoRESUMO
The effects of intracerebroventricular administration of the beta-amyloid peptide fragment Abeta(25-35) on the dynamics of the acquisition of a conditioned reflex in a Y maze were studied in Wistar and mongrel rats. The dynamics of decreases in the number of errors were assessed using an exponential mathematical model describing the transfer function of a first-order system in response to stepped inputs using non-linear regression analysis. This mathematical model provided a good approximation to the learning dynamics in inbred and mongrel mice. In Wistar rats, beta-amyloid impaired learning, with reduced memory between the first and second training sessions, but without complete blockade of learning. As a result, learning dynamics were no longer approximated by the mathematical model. At the same time, comparison of the number of errors in each training sessions between the control group of Wistar rats and the group given beta-amyloid showed no significant differences (Student's t test). This result demonstrates the advantage of regression analysis based on a mathematical model over the traditionally used statistical methods. In mongrel rats, the effect of beta-amyloid was limited to an a slowing of the process of learning as compared with control mongrel rats, with retention of the approximation by the mathematical model. It is suggested that mongrel animals have some kind of innate, genetically determined protective mechanism against the harmful effects of beta-amyloid.
Assuntos
Peptídeos beta-Amiloides/administração & dosagem , Aprendizagem/efeitos dos fármacos , Dinâmica não Linear , Fragmentos de Peptídeos/administração & dosagem , Animais , Comportamento Animal/efeitos dos fármacos , Condicionamento Psicológico/efeitos dos fármacos , Injeções Intraventriculares/métodos , Masculino , Aprendizagem em Labirinto/efeitos dos fármacos , Transtornos da Memória/induzido quimicamente , Ratos , Ratos WistarRESUMO
The neuroprotector effect of a new taurine derivative, 2-(1-phenylethyl)-aminoethanesulfonyl-2-propylamide hydrochloride, has been studied in rats with model compression spinal cord trauma. The drug favored restoration of the motor function of posterior extremities in rats with the model spinal cord trauma and significantly decreased the lethality in test animals. The taurine derivative normalized the energy metabolism, lipid peroxidation and antioxidant system in animals with spinal cord trauma. The neuroprotector effect of the new taurine derivative significantly exceeds the action of cerebrolysine.
Assuntos
Atividade Motora/efeitos dos fármacos , Fármacos Neuroprotetores/administração & dosagem , Recuperação de Função Fisiológica/efeitos dos fármacos , Compressão da Medula Espinal/tratamento farmacológico , Traumatismos da Medula Espinal/tratamento farmacológico , Taurina/administração & dosagem , Aminoácidos/administração & dosagem , Animais , Relação Dose-Resposta a Droga , Injeções Intraperitoneais , Masculino , Ratos , Compressão da Medula Espinal/patologia , Taurina/análogos & derivadosRESUMO
The survival of Chinese hamster cell was increased after the administration of cisteamine (at doses of 1,25; 2,5 and 5,0 mM/ml) in the culture for 15--20 min. before X-irradiation (600r). In the same experiment the protector decreased the rate of induced Ag-resistance gene mutations.
Assuntos
Cisteamina/farmacologia , Mutação/efeitos dos fármacos , Radiogenética , Animais , Azaguanina , Células Clonais , Cricetinae , Resistência a Medicamentos , Raios XRESUMO
New sublines of BFFR1 and BFFR3 cells were obtained as a result of prolonged cultivation of Chinese hamster cells of Blld-ii-FAF 28 line (clone 431) in the presence of increasing concentrations of methotrexate (MTX). The lines obtained were resistant to 200 and 300 mcM of MTX, respectively. Amplification of the gene for dihydrofolate reductase (DHFR), similar to normal DHFR gene in restriction patterns, was proved by blot-hybridization of the resistant cells' DNA with 32P-labeled plasmid DHFR-26. Correlation is shown between the extent of gene amplification and resistance of the cell lines. In situ hybridization of the metaphase chromosomes of resistant cells with 3H-DHFR-26 results in preferential binding of the label with the regions of marker chromosomes 2 and 5, containing long, so called differential staining regions which are known to be the places of localization of amplified genes.
Assuntos
Amplificação de Genes , Metotrexato/farmacologia , Tetra-Hidrofolato Desidrogenase/genética , Animais , Linhagem Celular , Cricetinae , Cricetulus , Resistência a Medicamentos , Cariotipagem , Hibridização de Ácido NucleicoRESUMO
The clinical significance of 201Tl myocardial perfusion scintigraphy was studied in 7 patients with abnormal branch of the left coronary artery from the pulmonary trunk and in 1 patient with that of the anterior interventricular artery from the pulmonary trunk. Radiological criteria for the defect were established on the basis of comparative analysis of the data of myocardial scintigraphy with those of electrocardiographic, coronary angiography and anatomic examinations. It was shown that it was feasible to make a differential diagnosis between abnormal left coronary artery branch and dilated cardiomyopathy and that the technique was of value for comparative assessment of the efficacy of various types of surgical treatment for abnormal left coronary artery ramus from the pulmonary trunk.
Assuntos
Anomalias dos Vasos Coronários/diagnóstico por imagem , Coração/diagnóstico por imagem , Artéria Pulmonar/anormalidades , Radioisótopos de Tálio , Tomografia Computadorizada de Emissão , Adolescente , Adulto , Angiografia , Criança , Pré-Escolar , Anomalias dos Vasos Coronários/diagnóstico , Eletrocardiografia , Feminino , Humanos , MasculinoRESUMO
Rat experiments have indicated that increased lipid peroxidation in gastric tissue occurs with formation of cryogenic gastric ulcers. The use of methacin alone, 1 mg/kg i.p., and in combination with proserinum, 0.1 mg/kg, s.c., metamisyl alone, 1 mg/kg, i. p., and in combination with galanthamine hydrobromide, 1 mg/kg, s. c., result in acceleration of reparative process in the rat gastric walls and normalization of gastric tissue malonic dialdehyde levels. Methacin in combination with proserinum has been found to be most beneficial.
Assuntos
Mucosa Gástrica/efeitos dos fármacos , Parassimpatolíticos/uso terapêutico , Parassimpatomiméticos/uso terapêutico , Animais , Avaliação Pré-Clínica de Medicamentos , Quimioterapia Combinada , Mucosa Gástrica/metabolismo , Peroxidação de Lipídeos/efeitos dos fármacos , Masculino , Malondialdeído/metabolismo , Ratos , Úlcera Gástrica/tratamento farmacológico , Úlcera Gástrica/metabolismo , Fatores de Tempo , Cicatrização/efeitos dos fármacosRESUMO
The possibility of using cycloferon (interferon inductor) for a complex treatment (in combination with the main drug solcoseryl possessing pronounced therapeutic properties) of duodenum ulcers was experimentally studied in male rats. The experiments showed a considerable difference in the interferon status of animals with model duodenum ulcers treated with cycloferon, solcoseryl, their combination, and placebo (control). The healing effect of solcoseryl administered in combination with cycloferon exceeded that of each component administered separately.