A Two-Year Bio-Agronomic and Chemotaxonomic Evaluation of Wild Sicilian Myrtle (Myrtus communis L.) Berries and Leaves.

A collection of nine Myrtus communis samples from different localities of Sicily was evaluated. Morphological traits and production characteristics have been chosen as parameters to arrange the samples into homogeneous groups and to identify the best biotypes for possible future agro-industrial exploitation. The plant material has been subjected to taxonomic characterization from biometric and phytochemical perspectives. Myrtle berries and leaves have been analyzed for their content in metabolites, applying a cascade extraction protocol for M. communis leaves and a single hydroalcoholic extraction for berries, whereas hydrodistillation procedures have been applied to obtain the essential oils from berries and leaves. The analyses of non-volatile components were carried out by LC-UV-DAD-ESI-MS. All the extracts were characterized by the presence of numerous polyphenols, namely highly hydroxylated flavonols such as quercetin and myricetin; and ellagic acid detected in all samples. In addition, myrtle berries contained nine different anthocyanins, namely delphinidin, petunidin, cyanidin and malvidin derivatives. The essential oils (EOs) were analyzed by a combination of GC-FID and GC/MS. A total of 33 and 34 components were fully characterized with the predominance of α-pinene, myrtenyl acetate, linalool, 1,8-cineole and linalyl acetate. All phytochemical profiles were subjected to cluster analyses, which allowed subdividing the myrtle samples in different chemical groups.

The use of constructed wetlands for the treatment and reuse of urban wastewater for the irrigation of two warm-season turfgrass species under Mediterranean climatic conditions.

Constructed wetlands (CWs) represent low-cost technology for the treatment and reuse of wastewater in urban areas. This study aimed to evaluate the pollutant removal efficiency of a CW system and to assess the effects of irrigation using treated urban wastewater on soil and on two warm-season turf species. The research was carried out in Sicily (Italy) on a pilot-scale horizontal subsurface flow system which was fed with treated urban wastewater following secondary treatment from an activated-sludge wastewater treatment plant. The pilot system was located in an open urban park and comprised two separate parallel planted units. Experimental fields of Cynodon dactylon (L.) Pers. and Paspalum vaginatum Sw. were set up close to the system and irrigated with both treated wastewater (TWW) and freshwater (FW). Irrigation with TWW did not result in a significant variation in soil pH and soil salinity in the topsoil. The turf species tolerated high sodium levels in the soil due to TWW irrigation. Savings in FW and mineral fertilizers were deemed significant. The results highlight the fact that use of CW systems for the treatment and reuse of wastewater can represent a sustainable way to obtain alternative water resources for turfgrass irrigation in urban areas.

Phytochemical, Ecological and Antioxidant Evaluation of Wild Sicilian Thyme: Thymbra capitata (L.) Cav.

In a broad survey conducted throughout the Sicily region, 45 different sites were identified where thyme grows wild. All the biotypes collected were classified as Thymbra capitata (L.) Cav. (syn. Thymus capitatus (L.) Hoffmanns. & Link). Cluster analysis based on the main morphological characteristics of the plant led to the division of the biotypes into 3 major groups. All samples were analyzed for their secondary phytochemical metabolites identified in the extracts and the essential oils. LC-UV-DAD/ESI-MS and GC-FID/GC-MS have been applied to characterize the extracts and the essential oils, respectively. In the extracts, 15 flavonoid derivatives with taxifolin-di-O-glucoside and thymusin as main components, and 2 organic acids, with a large predominance of rosmarinic acid, were identified. On the whole 37 compounds were fully characterized in the essential oils, carvacrol was identified as the main component with an average value of 73.93%. The total phenol content and the antioxidant activity of all phytochemical complexes were determined with the Folin-Ciocalteu (FC) assay, the UV radiation-induced peroxidation in liposomal membranes (UV-IP test), and the scavenging activity of superoxide radical (O2∙-).

Wild Sicilian rosemary: phytochemical and morphological screening and antioxidant activity evaluation of extracts and essential oils.

To identify the best biotypes, an extensive survey of Sicilian wild rosemary was carried out by collecting 57 samples from various sites, followed by taxonomic characterization from an agronomic perspective. All the biotypes collected were classified as Rosmarinus officinalis L. A cluster analysis based on the morphological characteristics of the plants allowed the division of the biotypes into seven main groups, although the characteristics examined were found to be highly similar and not area-dependent. Moreover, all samples were analyzed for their phytochemical content, applying an extraction protocol to obtain the nonvolatile components and hydrodistillation to collect the essential oils for the volatile components. The extracts were characterized by LC-UV-DAD/ESI-MS, and the essential oils by GC-FID and GC/MS analyses. In the nonvolatile fractions, 18 components were identified, namely, 13 flavones, two organic acids, and three diterpenes. In the volatile fractions, a total of 82 components were found, with as predominant components α-pinene and camphene among the monoterpene hydrocarbons and 1,8-cineole, camphor, borneol, and verbenone among the oxygenated monoterpenes. Cluster analyses were carried out on both phytochemical profiles, allowing the separation of the rosemary samples into different chemical groups. Finally, the total phenol content and the antioxidant activity of the essential oils and extracts were determined with the Folin-Ciocalteu (FC) colorimetric assay, the UV radiation-induced peroxidation in liposomal membranes (UV-IP test), and the scavenging activity of the superoxide radical (O$\rm{{_{2}^{{^\cdot} -}}}$). The present study confirmed that the essential oils and organic extracts of the Sicilian rosemary samples analyzed showed a considerable antioxidant/free radical-scavenging activity.

Modulation of cherry tomato performances in response to molybdenum biofortification and arbuscular mycorrhizal fungi in a soilless system.

Molybdenum (Mo) is a crucial microelement for both, humans and plants. The use of agronomic biofortification techniques can be an alternative method to enhance Mo content in vegetables. Concomitantly, arbuscular mycorrhizal fungi (AMF) application is a valuable strategy to enhance plant performances and overcome plant abiotic distresses such as microelement overdose. The aim of this research was to estimate the direct and/or indirect effects of Mo supply at four doses [0.0, 0.5 (standard dose), 2.0 or 4.0 µmol L-1], alone or combined with AMF inoculation, on plant performances. In particular, plant height and first flower truss emission, productive features (total yield, marketable yield and average marketable fruit weight) and fruit qualitative characteristics (fruit dry matter, soluble solids content, titratable acidity, ascorbic acid, lycopene, polyphenol, nitrogen, copper, iron and molybdenum) of an established cherry tomato genotype cultivated in soilless conditions were investigated. Moreover, proline and malondialdehyde concentrations, as well as Mo hazard quotient (HQ) in response to experimental treatments were determined. A split-plot randomized experimental block design with Mo dosages as plots and +AMF or -AMF as sub-plots was adopted. Data revealed that AMF inoculation enhanced marketable yield (+50.0 %), as well as some qualitative traits, such as fruit soluble solids content (SSC) (+9.9 %), ascorbic acid (+7.3 %), polyphenols (+2.3 %), and lycopene (+2.5 %). Molybdenum application significantly increased SSC, polyphenols, fruit Mo concentration (+29.0 % and +100.0 % in plants biofortified with 2.0 and 4.0 µmol Mo L-1 compared to those fertigated with the standard dose, respectively) and proline, whereas it decreased N (-25.0 % and -41.6 % in plants biofortified with 2.0 and 4.0 µmol Mo L-1 compared to those fertigated with the standard dose, respectively). Interestingly, the application of AMF mitigated the detrimental effect of high Mo dosages (2.0 or 4.0 µmol L-1). A pronounced advance in terms of plant height 45 DAT, fruit lycopene concentration and fruit Fe, Cu and Mo concentrations was observed when AMF treatment and Mo dosages (2.0 or 4.0 µmol Mo L-1) were combined. Plants inoculated or not with AMF showed an improvement in the hazard quotient (HQ) in reaction to Mo application. However, the HQ - for a consumption of 200 g day-1 of biofortified cherry tomato - remained within the safety level for human consumption. This study suggests that Mo-implementation (at 2.0 or 4.0 µmol L-1) combined with AMF inoculation could represent a viable cultivation protocol to enhance yield, produce premium quality tomato fruits and, concomitantly, improve Mo dose in human diet. In the light of our findings, further studies on the interaction between AMF and microelements in other vegetable crops are recommended.

Biomolecular characterization of wild sicilian oregano: phytochemical screening of essential oils and extracts, and evaluation of their antioxidant activities.

An extensive survey of wild Sicilian oregano was made. A total of 57 samples were collected from various sites, followed by taxonomic characterization from an agronomic perspective. Based on morphological and production characteristics obtained from the 57 samples, cluster analysis was used to divide the samples into homogeneous groups, to identify the best biotypes. All samples were analyzed for their phytochemical content, applying a cascade-extraction protocol and hydrodistillation, to obtain the non volatile components and the essential oils, respectively. The extracts contained thirteen polyphenol derivatives, i.e., four flavanones, seven flavones, and two organic acids. Their qualitative and quantitative characterization was carried out by LC/MS analyses. The essential oils were characterized using a combination of GC-FID and GC/MS analyses; a total of 81 components were identified. The major components of the oils were thymol, p-cymene, and γ-terpinene. Cluster analysis was carried out on both phytochemical profiles and resulted in the division of the oregano samples into different chemical groups. The antioxidant activity of the essential oils and extracts was investigated by the Folin-Ciocalteau (FC) colorimetric assay, by UV radiation-induced peroxidation in liposomal membranes (UV-IP test), and by determining the O(2)(∙-)-scavenging activity.

Iodine Biofortification and Seaweed Extract-Based Biostimulant Supply Interactively Drive the Yield, Quality, and Functional Traits in Strawberry Fruits.

The horticultural sector is seeking innovative and sustainable agronomic practices which could lead to enhanced yield and product quality. Currently, plant biofortification is recognized as a valuable technique to improve microelement concentrations in plant tissues. Among trace elements, iodine (I) is an essential microelement for human nutrition. Concomitantly, the application of biostimulants may improve overall plant production and quality traits. With the above background in mind, an experiment was designed with the aim of assessing the interactive impact of a seaweed extract-based biostimulant (SwE) (0 mL L-1 (served as control) or 3 mL L-1 (optimal dosage)) and 0, 100, 300, or 600 mg L-1 I on the growth parameters, yield, fruit quality, minerals, and functional characteristics of the tunnel-grown "Savana" strawberry. SwE foliar application improved the plant growth-related traits, total and marketable yield, fruit color parameters, soluble solids content, nitrogen (N), potassium (K), and magnesium (Mg) fruit concentrations. Furthermore, an enhancement in the fruit dry matter content, ascorbic acid, and I concentration in fruits was detected when the SwE supply interacted with a mild I dose (100 or 300 mg L-1). The research underlined that combining SwE application and I biofortification increased the strawberry yield and quality and enhanced the plant nutritional status variation, thereby, determining a boosted strawberry I tolerance.

Cytotoxic Effect Induced by Sicilian Oregano Essential Oil in Human Breast Cancer Cells.

Origanum vulgare L. is an aromatic plant that exerts antibacterial, antioxidant, anti-inflammatory, and antitumor activities, mainly due to its essential oil (EO) content. In this study, we investigated the possible mechanism underlying the in vitro antitumor activity of EO extracted by hydrodistillation of dried flowers and leaves of Origanum vulgare L. grown in Sicily (Italy) in MDA-MB-231 and MCF-7 breast cancer cell lines. Gas chromatography-mass spectrometry analysis of Oregano essential oil (OEO) composition highlighted the presence of twenty-six major phytocompounds, such as p-cymene, γ-terpinene, and thymoquinone p-acetanisole. OEO possesses strong antioxidant capacity, as demonstrated by the DPPH test. Our studies provided evidence that OEO reduces the viability of both MCF-7 and MDA-MB-231 cells. The cytotoxic effect of OEO on breast cancer cells was partially counteracted by the addition of z-VAD-fmk, a general caspase inhibitor. Caspases and mitochondrial dysfunction appeared to be involved in the OEO-induced death mechanism. Western blotting analysis showed that OEO-induced activation of pro-caspases-9 and -3 and fragmentation of PARP decreased the levels of Bcl-2 and Bcl-xL while increasing those of Bax and VDAC. In addition, fluorescence microscopy and cytofluorimetric analysis showed that OEO induces a loss of mitochondrial membrane potential in both cell lines. Furthermore, we tested the effects of p-cymene, γ-terpinene, thymoquinone, and p-acetanisole, which are the main components of OEO. Our findings highlighted that the effect of OEO on MDA-MB-231 and MCF-7 cells appears to be mainly due to the combination of different constituents of OEO, providing evidence of the potential use of OEO for breast cancer treatment.

Growth and development of succulent mixtures for extensive green roofs in a Mediterranean climate.

Green roof systems, aimed at reducing anthropic impact on the environment, are considered environmental mitigation technologies and adopted by many countries across the world to strengthen urban ecosystem services. This study evaluates two mixtures of succulent: one of Crassulaceae and the other of Aizoaceae, used in the creation of a continuous and homogenous plant groundcover in Mediterranean environments. To assess the species mixtures, the parameters plant height, growth index, cover percentage and flowering were observed. Hydrological observations were also carried out to evaluate the rainfall retained by the test system in any given month. All data were subjected to analysis of variance. Growth indicators in the study showed trends characteristic of xeric plants, which tend to slow down in dry, summer climate conditions to the point of halting plant vertical growth and ground cover development completely. The Aizocaeae mix, during the initial stage, showed prevalent horizontal growth, confirmed by greater a greater growth index (13,21) and cover percentage (45%) compared to Sedum (Growth index: 3,61; Cover: 36%). In contrast, the Sedum mix recorded greater vertical growth at the beginning (Sedum mixture: 7.53 cm; Aizoaceae mixture: 6,11 cm). During the final stages of observations, however, greater vertical growth in the Aizoaceae (7,88 cm) became apparent together with a recovery in horizontal growth in the Sedum (79%), albeit not sufficient to outperform the Aizoaceae mixture (87%). Flowering in the two mixtures occurred between late spring and late summer. The Sedum mixture guaranteed a longer flowering period (130 days) compared to the Aizoaceae (93 days), with a gradual start followed by steady flower emission. Regarding rainfall water retention, a comparison of the mixtures in late winter/early spring revealed that the Sedum performed best (44.9 L m2 vs 37.4 L m2), whilst the Aizoaceae outperformed the Sedum in Autumn (63 L m2 vs 55 L m2), in conjunction with favorable growth rates in both species mixtures. Both mixtures demonstrated satisfying results and are considered suited to a Mediterranean environment. Furthermore, based on the different growth rates of the species in the two test mixtures, this study suggests that new combinations of Sedum and Aizoaceae together might prove more resilient in Mediterranean environments.

Stand-Alone or Combinatorial Effects of Grafting and Microbial and Non-Microbial Derived Compounds on Vigour, Yield and Nutritive and Functional Quality of Greenhouse Eggplant.

The current research investigated the effects of endophytic fungi such as Trichoderma atroviride (Ta) or Ascophyllum nodosum seaweed extract (An) and their combination on growth, yield, nutritive and functional features, and mineral profile of 'Birgah' F1 eggplant either ungrafted, self-grafted or grafted onto the Solanum torvum rootstock. Eggplant exposed to An or An+Ta had a significant increase in root collar diameter 50 days after transplanting (RCD50), total yield (TY), marketable yield (MY), ascorbic acid (AA) content, Mg, Cu, and Zn concentration, and a reduction in glycoalkaloids (GLY) compared with the control. Furthermore, grafted plants had a higher TY, MY, number of marketable fruits (NMF), RCD50, AA, Cu, and Zn and a lower SSC, GLY, and Mg than the ungrafted plants. The combination of grafting and An+Ta significantly improved mean weight of marketable fruits (MF), plant height 50 days after transplanting (PH50), number of leaves 50 days after transplanting (NL50), fruit dry matter (FDM), chlorogenic acid (ClA), proteins, and K and Fe concentration. This combination also produced fruits of high premium quality as evidenced by the higher AA and ClA concentration, the lower GLY concentration, and an overall improved mineral profile.

Impact of Ecklonia maxima Seaweed Extract and Mo Foliar Treatments on Biofortification, Spinach Yield, Quality and NUE.

Seaweed extract (SE) application is a contemporary and sustainable agricultural practice used to improve yield and quality of vegetable crops. Plant biofortification with trace element is recognized as a major tool to prevent mineral malnourishment in humans. Mo deficiency causes numerous dysfunctions, mostly connected to central nervous system and esophageal cancer. The current research was accomplished to appraise the combined effect of Ecklonia maxima brown seaweed extract (SE) and Mo dose (0, 0.5, 2, 4 or 8 µmol L-1) on yield, biometric traits, minerals, nutritional and functional parameters, as well as nitrogen indices of spinach plants grown in a protected environment (tunnel). Head fresh weight (FW), ascorbic acid, polyphenols, N, P, K, Mg and nitrogen use efficiency (NUE) were positively associated with SE treatment. Moreover, head FW, head height (H), stem diameter (SD), ascorbic acid, polyphenols, carotenoids as well as NUE indices were enhanced by Mo-biofortification. A noticeable improvement in number of leaves (N. leaves), head dry matter (DM) and Mo concentration in leaf tissues was observed when SE application was combined with a Mo dosage of 4 or 8 µmol L-1. Overall, our study highlighted that E. maxima SE treatment and Mo supply can improve both spinach production and quality via the key enzyme activity involved in the phytochemical homeostasis of SE and the plant nutritional status modification resulting in an enhanced spinach Mo tolerance.

Effects of Irrigation with Different Sources of Water on Growth, Yield and Essential Oil Compounds in Oregano.

Aromatic plants can benefit from the use of treated wastewater to satisfy their water requirements, but the effects on the essential oil yield and quality need an assessment. The aims of this study were to assess the effects of freshwater and treated wastewater obtained from a Sicilian (Italy) pilot-scale horizontal subsurface flow constructed wetland system on plant growth and yield, essential oil yield and composition of oregano (Origanum vulgare ssp. hirtum (Link) Ietswaart) and soil characteristics. The system had a total surface area of 100 m2 and was planted with giant reed and umbrella sedge. An experimental open field of oregano was set up close to the system. Two years and two different sources of irrigation water were tested in a split-plot design for a two-factor experiment. Treated wastewater was characterized by higher values of mineral and organic constituents than freshwater. The results highlight that short-term irrigation with freshwater and treated wastewater, in both years, led to increased plant growth, dry weight and essential oil yield of oregano plants. However, it did not significantly affect the essential oil content and composition in comparison with the control. Furthermore, the year and source of irrigation water did not significantly vary the chemical composition of the soil. Our results suggest that treated wastewater can be considered an alternative to freshwater for the cultivation of oregano due to the fact that it does not greatly influence the yield quality and quantity of this species in the short-term.

Agro-Morphological Characterization of Sicilian Chili Pepper Accessions for Ornamental Purposes.

The species belonging to the genus Capsicum have been widely used as decorative vegetables, however only a few genotypes are available for this purpose. The goal of the present work was the agro-morphological characterization of several chili pepper accessions cultivated into different pot sizes (10, 14, 18 or 20 cm diameters). The agro-morphological characterization of 19 accessions was performed following IPGR (International Plant Genetic Resources Institute) descriptors: plant height (PH), plant canopy width (PCW), PH/PCW ratio, plant growth habit, plant visual quality, first flower emission, fruiting start, end of harvest, fruit number, fruit length, fruit width, fruit color at mature stage and fruit shape. Analysis of Variance (ANOVA) for all observed traits showed statistical significant differences among the genotypes tested. Results of the heat map complementarily secured the frequency of multiplicity highlighted from the ANOVA analysis. Furthermore, the present study pointed out that A33, A27, G1 and A1 chili pepper accessions achieved optimal performances in terms of plant visual quality, which is a crucial trait for ornamental purposes.

Effects of Environment and Sowing Time on Growth and Yield of Upland Cotton (Gossypium hirsutum L.) Cultivars in Sicily (Italy).

Cotton is one of the most important industrial crops in the world. Though widely cultivated in Sicily (Italy) in the past, cotton growth on the island has disappeared today due to a complex variety of agronomic, economic and socio-political reasons. In recent years, increased interest in natural fibers worldwide has led to a revival in cotton plants in the Mediterranean area. The aim of this paper was to assess the response of Gossypium hirsutum L. cultivars to different environments and sowing times. Elsa and Juncal were selected from the most promising cotton cultivars regarding earliness and productivity. Plants were tested with three sowing times and in two Sicilian environments. Cotton yield and yield components were significantly affected by experimental station, sowing time and cultivar. Lint yield of cultivars was 1.60 t ha-1 on average, and the highest value of 1.99 t ha-1 was obtained from an early sowing time. The three indices of agronomic earliness varied significantly based on treatments. In conclusion, the evaluation of response genotype-by-environment under different sowing times could represent a strategy to obtain optimal cotton seed and lint yields, although other general aspects, such as labor costs, land availability and capital resources, should be also considered when evaluating the reintroduction of the species in Sicily.

Celery (Apium graveolens L.) Performances as Subjected to Different Sources of Protein Hydrolysates.

The vegetable production sector is currently fronting several issues mainly connected to the increasing demand of high quality food produced in accordance with sustainable horticultural technologies. The application of biostimulants, particularly protein hydrolysates (PHs), might be favorable to optimize water and mineral uptake and plant utilization and to increase both production performance and quality feature of vegetable crops. The present study was carried out on celery plants grown in a tunnel to appraise the influence of two PHs, a plant-derived PH (P-PH), obtained from soy extract and an animal PH (A-PH), derived from hydrolyzed animal epithelium (waste from bovine tanneries) on yield, yield components (head height, root collar diameter, and number of stalks), mineral composition, nutritional and functional features, as well as the economic profitability of PHs applications. Fresh weight in A-PH and P-PH treated plants was 8.3% and 38.2% higher, respectively than in untreated control plants. However, no significant difference was found between A-PH treated plants and control plants in terms of fresh weight. Head height significantly increased by 5.5% and 16.3% in A-PH and P-PH treated plants, respectively compared with untreated control (p ≤ 0.05). N content was inferior in PHs treated plants than in untreated control. Conversely, K and Mg content was higher in A-PH and P-PH treated plants as compared to the untreated ones. Furthermore, A-PH and P-PH improved ascorbic acid content by 8.2% and 8.7%, respectively compared with the non-treated control (p ≤ 0.001). Our results confirmed, also, that PHs application is an eco-friendly technique to improve total phenolic content in celery plants. In support of this, our findings revealed that animal or plants PH applications increased total phenolics by 36.9% and 20.8%, respectively compared with untreated plants (p ≤ 0.001).

Research focusing on plant performance in constructed wetlands and agronomic application of treated wastewater - A set of experimental studies in Sicily (Italy).

Constructed wetlands are sustainable technologies for the treatment of wastewater. These biological systems have been widely studied throughout the world for more than 30 years; however, most studies have focused on the effects of design and engineering on pollutant removal from wastewater. Undoubtedly, agro-technical aspects have been given too little consideration by research. This paper reports the main results of a set of experiments carried out on two pilot horizontal subsurface flow systems in Sicily (Italy). Festuca, Lolium and Pennisetum spp. in combination and three emergent macrophytes-Arundo donax L., Cyperus alternifolius L. and Typha latifolia L.-alone, were assessed. The aim of the study was to demonstrate that, under predetermined hydraulic and design conditions, the choice of plant species and the management of the vegetation can significantly affect the pollutant removal performance of constructed wetlands. In addition, wastewater (after treatment) can also be used for agricultural purposes leading to increased sustainability in agricultural systems. Arundo and Typha-planted units performed better than Cyperus-planted units in terms of chemical, physical and microbiological contaminant removal. All the species adapted extremely well to wetland conditions. Polyculture systems were found to be more efficient than monocultures in the removal of dissolved organic compounds. The reuse of treated wastewater for the irrigation of open fields and horticultural crops led to significant savings in the use of freshwater and fertilizers. The results of physical-energy characterization of A. donax above-ground plant residues and pellets highlighted the fact that a constructed wetland could also be a potential source of bioenergy.

Agronomic, metabolomic and lipidomic characterisation of Sicilian Origanum vulgare (L.) ecotypes.

Although Origanum vulgare (L.) has been deeply analysed at phytochemical level, poor knowledge is available regarding non-volatile compounds such as lipids. The aim of this work was to characterise five wild Sicilian Origanum ecotypes from an agronomic, metabolomic and lipidomic perspective. Serradifalco presented higher dry weight and inflorescences/plant than the others while Favara had a significantly higher number of branches per plant and more extensive flowered stratum. Metabolomic analysis, performed with LC-MS-TOF, allowed a preliminary characterisation of the non-volatile metabolome of the five oregano ecotypes Origanum vulgare ssp. hirtum. Twenty-five metabolites were identified belonging to organic acids, amino acids, lysophosphatidylcholines, carnithines, nucleic bases and lysophosphatidylethanolamines. Lipidomic analysis identified 115 polar plant membrane glycerolipid species. Thirteen of them were differentially present in the two chosen ecotypes. The role of these metabolites in plant physiology from a qualitative and pharmacological point of view was discussed.

Effects of Plant Density on the Number of Glandular Trichomes and on Yield and Quality of Essential Oils from Oregano.

Plants yields are influenced by agronomic techniques. Plant density is a complex issue and extremely important when maximizing both crop quality, and biomass and essential oil yields. Plants belonging to the Origanum vulgare subspecies hirtum (Link) Ietswaart were grown adopting four types of plant density and were characterized in biometric and chemical terms. The samples were analyzed using the ANOVA (Principal Component Analysis) statistical method regarding biometric aspects, EO yield and peltate hair density. Essential oil (EO) was extracted by hydrodistillation and analyzed using GC-FID and GC-MS. GC-FID and GC-MS analysis led to the identification of 45 compounds from the EO. Plant density affected production both in terms of biomass and EO. However, it was not found to have affected peltate glandular trichome density or EO quality.

A survey of wild plant species for food use in Sicily (Italy) - results of a 3-year study in four Regional Parks.

BACKGROUND: This paper illustrates the results of a study carried out in four Regional Parks of Sicily (Italy), concerning traditional knowledge on food use of wild plant species. The main aims of the paper were: (i) to verify which wild plant species are used for food purpose in the local culture based on information provided by elderly inhabitants (ii) to verify the presence of wild plant species which have not been cited for food use in previous studies in the Mediterranean area (iii) to determine how many of the most frequently cited wild plant species are cultivated by the local population in the four Sicilian Parks. METHODS: Semi-structured interviews were carried out in the local communities of the four Regional Parks between 2007 and 2010. A total of 802 people over the age of 60 were interviewed. Cultural Importance Index was used to evaluate the level of importance given to any wild plant species as a food in the local culture. The level of appreciation of the wild plant species and the possible effects of wild plants on human health were also investigated. RESULTS: Local communities currently use a total number of 119 wild species for food purposes. Asteraceae and Brassicaceae were the most represented botanical families. In each of the four Sicilian Parks, Cichorium intybus L. and Foeniculum vulgare Mill. obtained the highest Cultural Importance Index values. Sixty-four species were indicated as also having medicinal properties. Leaves and other aerial plant parts were the parts most-used for the preparation of traditional recipes. CONCLUSIONS: The research shows that the level of traditional knowledge on the food uses of wild plant species in the study area is poor. The food uses of plants which are most likely to survive over time are those at the interface of food and medicine. Further agronomic studies are needed for a number of species with a view to introducing them as a crop into non-intensive agricultural systems.

Agronomical and chemical characterisation of Thymbra capitata (L.) Cav. biotypes from Sicily, Italy.

In this study, the agronomical and chemical characterisation of 13 Sicilian biotypes of Thymbra capitata (L.) Cav., grown under the same agricultural and environmental condition, are reported. The main morpho-productive parameters and quali-quantitative profile of essential oils (EOs) were determined. The EOs were analysed by gas chromatography-flame ionisation detector and gas chromatography-mass spectrometry. Hierarchical cluster analysis and principal component analysis statistical methods were used to group biotypes according to the EOs chemical composition. The EO yield ranged between 4.6 and 8.1 (v/w). A total of 38 EO compounds have been identified. The compounds mostly represented were α-pinene, myrcene, α-terpinene, p-cymene, γ-terpinene, borneol, carvacrol and ß-caryophyllene. In all biotypes, the carvacrol (67.4-79.5%) was the main compound, confirming that T. capitata is a carvacrol chemotype. The results showed that all Sicilian Thymbra biotypes have a good adaptation to the climatic conditions of the test environment.