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1.
Can Vet J ; 59(1): 82-84, 2018 01.
Artigo em Inglês | MEDLINE | ID: mdl-29302107

RESUMO

A 1-year-old spayed female cat was presented with tachypnea and a protrusion on the ventral thorax. Radiography revealed ventral sternal subluxation between the 6th and 7th sternebrae. There was no evidence of respiratory distress, even after follow-up, and a conservative management approach was successful in this healthy, young, indoor cat.


Subluxation sternale aiguëchez un chat d'intérieur. Une chatte stérilisée âgée de 1 an a été présentée avec de la tachypnée et une protrusion sur le thorax ventral. La radiographie a révélé une subluxation sternale ventrale entre le 6e et le 7e sternèbre. Il n'y avait pas de signe de détresse respiratoire, même après le suivi, et une approche de gestion prudente a été efficace chez ce jeune chat d'intérieur en santé.(Traduit par Isabelle Vallières).


Assuntos
Doenças do Gato/etiologia , Luxações Articulares/veterinária , Esterno/lesões , Animais , Gatos , Feminino , Luxações Articulares/diagnóstico , Luxações Articulares/terapia
2.
Methods Mol Biol ; 1074: 51-67, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23975805

RESUMO

Embryonic stem (ES) cells are derived from the inner cell masses of preimplantation embryos. ES cells are pluripotent cells with the capacity for long-term propagation and broad differentiation plasticity. These cells have an exceptional functional feature in that they can differentiate into all tissues and organs, including germ cells. Established ES cell lines have been generated in mouse, human, and nonhuman primate but derivation of ES cells in farm animals has been problematic. Several ES-like cell lines from farm animals have been reported to exhibit properties of pluripotency in vitro. However, only a few of them morphologically resemble ES cells, or express markers that are associated with established ES cell lines from mouse and humans. Methods for derivation, propagation, and differentiation of ES cells from domestic animals have not been fully established. In this chapter, we describe methods for isolation of goat ES (gES) cell lines from in vivo-derived blastocysts and characterization of markers indicative of pluripotency. In addition, we outline differentiation of gES cells into all three germ layers in vivo by forming teratomas as a hallmark of pluripotency.


Assuntos
Blastocisto/citologia , Técnicas de Cultura de Células/métodos , Células-Tronco Embrionárias/citologia , Cabras , Animais , Embrião de Mamíferos/citologia , Humanos , Cariotipagem , Camundongos , Células-Tronco Pluripotentes/citologia , Células-Tronco Pluripotentes/metabolismo , Teratoma/metabolismo
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