Linking magnetite in the abdomen of honey bees to a magnetoreceptive function.

Previous studies of magnetoreception in honey bees, Apis mellifera, focused on the identification of magnetic material, its formation, the location of the receptor and potential underlying sensory mechanisms, but never directly linked magnetic material to a magnetoreceptive function. In our study, we demonstrate that ferromagnetic material consistent with magnetite plays an integral role in the bees' magnetoreceptor. Subjecting lyophilized and pelletized bee tagmata to analyses by a superconducting quantum interference device generated a distinct hysteresis loop for the abdomen but not for the thorax or the head of bees, indicating the presence of ferromagnetic material in the bee abdomen. Magnetic remanence of abdomen pellets produced from bees that were, or were not, exposed to the 2.2-kOe field of a magnet while alive differed, indicating that magnet exposure altered the magnetization of this magnetite in live bees. In behavioural two-choice field experiments, bees briefly exposed to the same magnet, but not sham-treated control bees, failed to sense a custom-generated magnetic anomaly, indicating that magnet exposure had rendered the bees' magnetoreceptor dysfunctional. Our data support the conclusion that honey bees possess a magnetite-based magnetoreceptor located in the abdomen.

Honey bees possess a polarity-sensitive magnetoreceptor.

Honey bees, Apis mellifera, exploit the geomagnetic field for orientation during foraging and for alignment of their combs within hives. We tested the hypothesis that honey bees sense the polarity of magnetic fields. We created an engineered magnetic anomaly in which the magnetic field generally either converged toward a sugar reward in a watch glass, or away from it. After bees in behavioral field studies had learned to associate this anomaly with a sugar water reward, we subjected them to two experiments performed in random order. In both experiments, we presented bees with two identical sugar water rewards, one of which was randomly marked by a magnetic field anomaly. During the control experiment, the polarity of the magnetic field anomaly was maintained the same as it was during the training session. During the treatment experiment, it was reversed. We predicted that bees would not respond to the altered anomaly if they were sensitive to the polarity of the magnetic field. Our findings that bees continued to respond to the magnetic anomaly when its polarity was in its unaltered state, but did not respond to it when its polarity was reversed, support the hypothesis that honey bees possess a polarity-sensitive magnetoreceptor.

Size does not matter, but features do: Clark's nutcrackers (Nucifraga columbiana) weigh features more heavily than geometry in large and small enclosures.

Two groups of Clark's nutcrackers (Nucifraga columbiana) were trained to locate a hidden goal which was consistently located at one corner of a fully enclosed rectangular environment with distinctive cues available at each corner. One group was trained in a small enclosure, whereas the second group was trained in a large enclosure. Once the birds were showing accurate search behavior, they were presented with non-reinforced tests in either the same sized environment as training or the novel sized environment, as well as in a square-shaped environment. The birds were able to accurately search at the two geometrically correct corners when the four distinctive features were removed showing that they had encoded geometry. Although accuracy was greater when tested in the same sized environment as during training, accuracy was above chance in both environments. Regardless of the size of training enclosure both groups showed primary control by features along with secondary control by geometry. Furthermore, when the features and geometric cues provided conflicting information as to the goal location, both groups weighed featural cues over geometry, and this was independent of whether the size of the testing environment was maintained or manipulated. These results show that for Clark's nutcrackers the size of the environment had little effect on the weighing of featural and geometric cues. Furthermore, although nutcrackers encoded both features and geometry, when spatial cues provided discrepant information as to the goal location, nutcrackers relied primarily on features. This article is part of a Special Issue entitled: CO3 2013.

Does the Earth's magnetic field serve as a reference for alignment of the honeybee Waggle dance?

The honeybee (Apis mellifera) waggle dance, which is performed inside the hive by forager bees, informs hive mates about a potent food source, and recruits them to its location. It consists of a repeated figure-8 pattern: two oppositely directed turns interspersed by a short straight segment, the "waggle run". The waggle run consists of a single stride emphasized by lateral waggling motions of the abdomen. Directional information pointing to a food source relative to the sun's azimuth is encoded in the angle between the waggle run line and a reference line, which is generally thought to be established by gravity. Yet, there is tantalizing evidence that the local (ambient) geomagnetic field (LGMF) could play a role. We tested the effect of the LGMF on the recruitment success of forager bees by placing observation hives inside large Helmholtz coils, and then either reducing the LGMF to 2% or shifting its apparent declination. Neither of these treatments reduced the number of nest mates that waggle dancing forager bees recruited to a feeding station located 200 m north of the hive. These results indicate that the LGMF does not act as the reference for the alignment of waggle-dancing bees.