A Modified Approach for in Situ Chemical Oxidation Coupled to Biodegradation Enhances Light Nonaqueous Phase Liquid Source-Zone Remediation.

Field and batch experiments were conducted to assess whether a modified approach for in situ chemical oxidation (ISCO) (with MgO2 and Fe2O3 particles recovered from acid mine drainage treatment) can enhance LNAPL (light nonaqueous phase liquid) dissolution and produce bioavailable soluble compounds. This modified ISCO approach was coupled to biodegradation to further remove residual compounds by microbially mediated processes. Pure palm biodiesel (B100) was chosen to represent a poorly water-soluble compound that behaves like LNAPLs, and 100 L was released to a 2 m2 area excavated down to the water table. A past adjacent B100-field experiment under natural attenuation was conducted as a baseline control. Results demonstrated the enhancement of organic compound dissolution and production of soluble compounds due to the modified in situ chemical oxidation. The slow release of H2O2 by MgO2 decomposition (termed partial chemical oxidation) and production of soluble compounds allowed the stimulation of microbial growth and promoted a beneficial response in microbial communities involved in oxidized biodiesel compound biodegradation. This is the first field experiment to demonstrate that this modified ISCO approach coupled to biodegradation could be a feasible strategy for the removal of poorly water-soluble compounds (e.g., biodiesel) and prevent the long-term effects generally posed in source zones.

Combined iron and sulfate reduction biostimulation as a novel approach to enhance BTEX and PAH source-zone biodegradation in biodiesel blend-contaminated groundwater.

The use of biodiesel as a transportation fuel and its growing mandatory blending percentage in diesel increase the likelihood of contaminating groundwater with diesel/biodiesel blends. A 100L-field experiment with B20 (20% biodiesel and 80% diesel, v/v) was conducted to assess the potential for the combined biostimulation of iron and sulfate reducing bacteria to enhance BTEX and PAH biodegradation in a diesel/biodiesel blend-contaminated groundwater. A B20 field experiment under monitored natural attenuation (MNA) was used as a baseline control. Ammonium acetate and a low-cost and sustainable product recovered from acid mine drainage treatment were used to stimulate iron and sulfate-reducing conditions. As a result, benzene and naphthalene concentrations (maximum concentrations were 28.1µgL-1 and 10.0µgL-1, respectively) remained lower than the MNA experiment (maximum concentrations were 974.7µgL-1 and 121.3µgL-1, respectively) over the whole experiment. Geochemical changes were chronologically consistent with the temporal change of the predominance of Geobacter and GOUTA19 which might be the key players responsible for the rapid attenuation of benzene and naphthalene. To the best of our knowledge, this is the first field experiment to demonstrate the potential for the combined iron and sulfate biostimulation to enhance B20 source-zone biodegradation.