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The fabrication of formamidinium lead iodide (FAPbI3) perovskite solar cells (PSCs) involves the addition of methylammonium chloride (MACl) to promote low-temperature α-phase formation and grain growth. However, as the added MACl deprotonates and volatilizes into methylamine (MA0) and HCl for removal, MA0 can chemically interact with formamidinium (FA+), forming methyl formamidinium (MFA+) as a byproduct. Despite its significance, the chemical interactions among FAPbI3 perovskites, MACl additives, and their byproducts remain poorly understood. Our findings reveal that the FA+ and MA0 reaction primarily yields a mixture of cis/trans-N-methyl formamidinium iodide (MFAI) isomers, with cis-MFAI prevailing as the dominant species. Moreover, MFAI subsequently reacts with PbI2 to yield fully formed cis-MFAPbI3 2H-phase perovskite. We elucidated the effects of MFAI on the crystal growth, phase stability, and band gap of formamidine-based perovskites through the growth of single crystals. This research offers valuable insights into the role of these byproducts in influencing the efficiency and long-term stability of future PSCs.
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A few metal-organic frameworks (MOFs), which typically use strong acids as proton sources, display superprotonic conductivity (≈10-1 â S cm-1 ); however, they are rare due to the instability of MOFs in highly acidic conditions. For the first time, we report superprotonic conductivity using a moderately acidic guest, zwitterionic sulfamic acid (HSA), which is encapsulated in MOF-808 and MIL-101. HSA acts not only as a proton source but also as a proton-conducting medium due to its extensive hydrogen bonding ability and zwitterion effect. A new sustained concentration gradient method results in higher HSA encapsulation compared to conventional methods, producing 10HSA@MOF-808-(bSA)2 and 8HSA@MIL-101. These MOFs show impressive superprotonic conductivity of 2.47×10-1 and 3.06×10-1 â S cm-1 , respectively, at 85 °C and 98 % relative humidity, and maintain stability for 7â days.
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A new synthetic approach for tunable mesoporous metal-organic frameworks (MeMs) is developed. In this approach, mesopores are created in the process of heat conversion of highly mosaic metal-organic framework (MOF) crystals with non-interpenetrated low-density nanocrystallites into MOF crystals with two-fold interpenetrated high-density nanocrystallites. The two-fold interpenetration reduces the volume of the nanocrystallites in the mosaic crystal, and the accompanying localized agglomeration of the nanocrystallites results in the formation of mesopores among the localized crystallite agglomerates. The pore size can be easily modulated from 7 to 90 nm by controlling the heat treatment conditions, that is, the aging temperature and aging time. Various proteins can be encapsulated in the MeM, and immobilized enzymes show catalyst activity comparable to that of the free native enzymes. Immobilized ß-galactosidase is recyclable and the enzyme activity of the immobilized catalase is maintained after exposure to high temperatures and various organic solvents.
Assuntos
Enzimas Imobilizadas , Estruturas Metalorgânicas , Catálise , Enzimas Imobilizadas/metabolismo , Estruturas Metalorgânicas/química , TemperaturaRESUMO
A metal-organic framework (MOF) having superprotonic conductivity, MOF-808, is prepared by modulating the binding mode of the sulfamate (SA) moieties grafted onto the metal clusters. The activation of the SA-grafted MOF-808 at 150 °C changes the binding mode of the grafted SA from monodentate to bridging bidentate, thus converting the neutral amido (-S-NH2 ) moiety of the grafted SA to the more acidic cationic sulfiliminium (-S=NH2+ ) moiety. Further, the acidic sulfiliminium moiety of MOF-808-4SA-150 results in more efficient proton conduction than the amido moiety of MOF-808-4SA-60. At 60 °C and 95 % relative humidity, MOF-808-4SA-150 is found to have a proton conductivity of 7.89×10-2 â S cm-1 , which is more than 30-times higher than that of MOF-808-4SA-60. Moreover, this superprotonic conductivity is well maintained over 1000â cycles of conductivity measurements and for similar cyclic measurements each day for seven days.
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MOF-74 is one of the most explored metal-organic frameworks (MOFs), but its functionalization is limited to the dative post-synthetic modification (PSM) of the monodentate solvent site. Owing to the nature of the organic ligand and framework structure of MOF-74, the covalent PSM of MOF-74 is very demanding. Herein, we report, for the first time, the covalent PSM of amine-tagged defective Ni-MOF-74, which is prepared by deâ novo solvothermal synthesis by using aminosalicylic acid as a functionalized fragmented organic ligand. The covalent PSM of the amino group generates metal binding sites, and subsequent post-synthetic metalation with PdII ions affords the PdII -incorporated Ni-MOF-74 catalyst. This catalyst exhibits highly efficient, size-selective, and recyclable catalytic activity for the Suzuki-Miyaura cross-coupling reaction. This strategy is also useful for the covalent modification of amine-tagged defective Ni2 (DOBPDC), an expanded analogue of MOF-74.
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Fragment engineering of monoclonal antibodies (mAbs) has emerged as an excellent paradigm to develop highly efficient therapeutic and/or diagnostic agents. Engineered mAb fragments can be economically produced in bacterial systems using recombinant DNA technologies. In this work, we established recombinant production in Escherichia coli for monovalent antigen-binding fragment (Fab) adopted from a clinically used anticancer mAB drug cetuximab targeting epidermal growth factor receptor (EGFR). Recombinant DNA constructs were designed to express both polypeptide chains comprising Fab in a single vector and to secrete them to bacterial periplasmic space for efficient folding. Particularly, a C-terminal engineering to confer an interchain disulfide bond appeared to be able to enhance its heterodimeric integrity and EGFR-binding activity. Conformational relevance of the purified final product was validated by mass spectrometry and crystal structure at 1.9 Å resolution. Finally, our recombinant cetuximab-Fab was found to have strong binding affinity to EGFR overexpressed in human squamous carcinoma model (A431) cells. Its binding ability was comparable to that of cetuximab. Its EGFR-binding affinity was estimated at approximately 0.7 nM of Kd in vitro, which was quite stronger than the binding affinity of natural ligand EGF. Hence, the results validate that our construction could serve as an efficient platform to produce a recombinant cetuximab-Fab with a retained antigen-binding functionality.
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Antineoplásicos/metabolismo , Cetuximab/metabolismo , Receptores ErbB/antagonistas & inibidores , Escherichia coli/metabolismo , Fragmentos Fab das Imunoglobulinas/metabolismo , Proteínas Recombinantes/metabolismo , Relação Estrutura-Atividade , Antineoplásicos/química , Linhagem Celular Tumoral , Cetuximab/química , Cetuximab/genética , Cristalografia por Raios X , Escherichia coli/genética , Humanos , Fragmentos Fab das Imunoglobulinas/química , Fragmentos Fab das Imunoglobulinas/genética , Espectrometria de Massas , Ligação Proteica , Conformação Proteica , Engenharia de Proteínas , Proteínas Recombinantes/química , Proteínas Recombinantes/genéticaRESUMO
α-Synuclein (α-Syn) is an intrinsically disordered protein, whose fibrillar aggregates are associated with the pathogenesis of Parkinson's disease. α-Syn associates with lipid membranes and forms helical structures upon membrane binding. In this study, we explored the helix formation of α-Syn in solution containing trifluoroethanol using small-angle X-ray scattering and electrospray ionization ion mobility mass spectrometry. We then investigated the structural transitions of α-Syn to helical structures via association with large unilamellar vesicles as model lipid membrane systems. Hydrogen-deuterium exchange combined with electrospray ionization mass spectrometry was further utilized to understand the details of the regional interaction mechanisms of α-Syn with lipid vesicles based on the polarity of the lipid head groups. The characteristics of the helical structures were observed with α-Syn by adsorption onto the anionic phospholipid vesicles via electrostatic interactions between the N-terminal region of the protein and the anionic head groups of the lipids. α-Syn also associates with zwitterionic lipid vesicles and forms helical structures via hydrophobic interactions. These experimental observations provide an improved understanding of the distinct structural change mechanisms of α-Syn that originate from different regional interactions of the protein with lipid membranes and subsequently provide implications regarding diverse protein-membrane interactions related to their fibrillation kinetics.
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Membrana Celular/metabolismo , alfa-Sinucleína/química , alfa-Sinucleína/metabolismo , Membrana Celular/química , Bicamadas Lipídicas/química , Bicamadas Lipídicas/metabolismo , Fosfolipídeos/química , Fosfolipídeos/metabolismo , Ligação Proteica , Estrutura Secundária de Proteína , Lipossomas Unilamelares/química , Lipossomas Unilamelares/metabolismoRESUMO
Targeted drug delivery systems based on metal-organic frameworks (MOFs) have progressed tremendously since inception and are now widely applicable in diverse scientific fields. However, translating MOF agents directly to targeted drug delivery systems remains a challenge due to the biomolecular corona phenomenon. Here, we observed that supramolecular conjugation of antibodies to the surface of MOF particles (MOF-808) via electrostatic interactions and coordination bonding can reduce protein adhesion in biological environments and show stealth shields. Once antibodies are stably conjugated to particles, they were neither easily exchanged with nor covered by biomolecule proteins, which is indicative of the stealth effect. Moreover, upon conjugation of the MOF particle with specific targeted antibodies, namely, anti-CD44, human epidermal growth factor receptor 2 (HER2), and epidermal growth factor receptor (EGFR), the resulting hybrid exhibits an augmented targeting efficacy toward cancer cells overexpressing these receptors, such as HeLa, SK-BR-3, and 4T1, as evidenced by flow cytometry. The therapeutic effectiveness of the antibody-conjugated MOF (anti-M808) was further evaluated through in vivo imaging and the assessment of tumor inhibition effects using IR-780-loaded EGFR-M808 in a 4T1 tumor xenograft model employing nude mice. This study therefore provides insight into the use of supramolecular antibody conjugation as a promising method for developing MOF-based drug delivery systems.
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Estruturas Metalorgânicas , Camundongos Nus , Estruturas Metalorgânicas/química , Humanos , Animais , Camundongos , Sistemas de Liberação de Medicamentos , Anticorpos/química , Anticorpos/imunologia , Receptores ErbB/imunologia , Receptores ErbB/metabolismo , Linhagem Celular Tumoral , Células HeLa , Camundongos Endogâmicos BALB C , Antineoplásicos/química , Antineoplásicos/farmacologia , Receptor ErbB-2/imunologia , Receptor ErbB-2/metabolismo , FemininoRESUMO
Malaria, a major burden of disease caused by parasites of the genus Plasmodium, is widely spread in tropical and subtropical regions. Here, we have successfully developed a diagnostic technique for malaria. The proposed method is based on the interaction among the Plasmodium lactate dehydrogenase (pLDH), which is a biomarker for malaria, and pL1 aptamer against Plasmodium vivax lactate dehydrogenase (PvLDH) and Plasmodium falciparum lactate dehydrogenase (PfLDH). In addition, the cationic polymers, poly(diallyldimethylammonium chloride) (PDDA) and poly(allylamine hydrochloride) (PAH), aggregate gold nanoparticles (AuNPs) that should be possible to observe the change in color from red to blue, which depends on the concentration of pLDH. Using this aptasensor, pLDH proteins were successfully detected with low detection limits. Moreover, the specificity test proved that the aptasenor is very specific in targeting proteins over other interfering proteins. In addition, the pLDH from infected blood samples of the two main species of malaria were also detected. The limits of detection for P. vivax were determined as 80 parasites/µl for PDDA and 74 parasites/µl for PAH. The aptasenor has great advantages that can simply and rapidly diagnose malaria. Thus, the developed aptasensor for detection of pLDH can offer an effective and sensitive diagnosis of malaria.
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Aptâmeros de Nucleotídeos/metabolismo , Técnicas Biossensoriais/métodos , Colorimetria/métodos , Ouro/química , Malária/diagnóstico , Nanopartículas Metálicas/química , Polímeros/química , Aptâmeros de Nucleotídeos/química , Humanos , L-Lactato Desidrogenase/sangue , L-Lactato Desidrogenase/química , L-Lactato Desidrogenase/metabolismo , Malária/sangue , Modelos Moleculares , Conformação de Ácido Nucleico , Plasmodium/enzimologia , Plasmodium/isolamento & purificação , Plasmodium/fisiologia , Conformação ProteicaRESUMO
Tumor hypoxia poses a significant challenge in photodynamic therapy (PDT), which uses molecular oxygen to produce reactive oxygen species upon light excitation of a photosensitizer. For hypoxia mitigation, an enzyme catalase (CAT) can be beneficially used to convert intracellular hydrogen peroxide to molecular oxygen, but its utility is significantly limited due to the intrinsic membrane impermeability. Herein, we present direct integration of CAT into the outer surface of unmodified metal-organic framework (MOF) nanoparticles (NPs) via supramolecular interactions for effective cellular entry of CAT and consequent enhancement of PDT. The results demonstrated that CAT-loaded MOF NPs could successfully enter hypoxic cancer cells, after which the intracellularly delivered CAT molecules became dissociated from the MOF surface to efficiently initiate the oxygen generation and PDT process along with a co-delivered photosensitizer IR780. This achievement suggests that our protein-MOF integration strategy holds great potential in biomedical studies to overcome tumor hypoxia as well as to efficiently deliver biomolecular cargos.
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In this study, spinel cobalt oxide (Co3O4) nanoparticles without combining with any other metal atoms have been decorated through the influence of two hard templating agents, viz., zeolite-Y and carboxy-functionalized multiwalled carbon nanotubes (COOH-MWCNT). The adornment of the Co3O4 nanoparticles, through the combined impact of the aluminosilicate and carbon framework has resulted in quantum interference, causing the reversal of signatory Raman peaks of Co3O4. Apart from the construction of small Co3O4 nanoparticles at the interface of the two matrices, the particles were aligned along the direction of COOH-MWCNT. The catalyst Co3O4-Y-MWCNT exhibited excellent catalytic activity toward the methanol oxidation reaction (MOR) in comparison to Co3O4-Y, Co3O4-MWCNT, and bared Co3O4 with the current density of 0.92 A mg-1 at an onset potential of 1.33 V versus RHE. The material demonstrated persistent electrocatalytic activity up to 300 potential cycles and 20,000 s without substantial current density loss. High surface area of zeolite-Y in combination with the excellent conductivity of the COOH-MWCNT enhanced the electrocatalytic performance of the catalyst. The simplicity of synthesis, scale-up, and remarkable electrocatalytic activity of the catalyst Co3O4-Y-MWCNT provided an effective way toward the development of anode materials for direct methanol fuel cells.
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This research explores the alteration of metal-organic frameworks (MOFs) using a method called postsynthetic metal exchange. We focus on the shift from a Zn-based MOF containing a [Zn4O(COO)6] secondary building unit (SBU) of octahedral site symmetry (ANT-1(Zn)) to a Fe-based one with a [Fe3IIIO(COO)6]+ SBU of trigonal prismatic site symmetry (ANT-1(Fe)). The symmetry-mismatched SBU transformation cleverly maintains the MOF's overall structure by adjusting the conformation of the flexible 1,3,5-benzenetribenzoate linker to alleviate the framework strain. The process triggers a decrease in the framework volume and pore size alongside a change in the framework's charge. These alterations influence the MOF's ability to adsorb gas and dye. During the transformation, core-shell MOFs (ANT-1(Zn@Fe)) are formed as intermediate products, demonstrating unique gas sorption traits and adjusted dye adsorption preferences due to the structural modifications at the core-shell interface. Heteronuclear clusters, located at the framework interfaces, enhance the heat of CO2 adsorption. Furthermore, they also influence the selectivity of the dye size. This research provides valuable insights into fabricating novel MOFs with unique properties by modifying the SBU of a MOF with flexible organic linkers from one site symmetry to another.
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We have designed multiple detection systems for the DNA strand exchange process. Thermostable Thermotoga maritima recombinase A (TmRecA), a core protein in homologous recombination, and DNAzyme, a catalytic DNA that can cleave a specific DNA sequence, are introduced in this work. In a colorimetric method, gold nanoparticles (AuNPs) modified with complementary DNAs (cDNAs) were assembled by annealing. Aggregated AuNPs were then separated irreversibly by TmRecA and DNAzyme, leading to a distinct color change in the particles from purple to red. For the case of fluorometric detection, fluorescein isothiocyanate (FITC)-labeled DNA as a fluorophore and black hole quencher 1 (BHQ1)-labeled DNA as a quencher were used; successful strand exchange was clearly detected by variations in fluorescence intensity. In addition, alterations in the impedance of a gold electrode with immobilized DNA were employed to monitor the regular exchange of DNA strands. All three methods provided sufficient evidence of efficient strand exchange reactions and have great potential for applications in the monitoring of recombination, discovery of new DNAzymes, detection of DNAzymes, and measurement of other protein activities.
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Técnicas Biossensoriais/métodos , DNA Catalítico , Recombinases Rec A , DNA/química , DNA/genética , Técnicas Eletroquímicas , Fluoresceína-5-Isotiocianato , Corantes Fluorescentes , Fluorometria , Ouro , Nanopartículas Metálicas , Recombinases Rec A/genética , Recombinases Rec A/metabolismo , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Thermotoga maritima/enzimologia , Thermotoga maritima/genéticaRESUMO
Recently, aptamers have attracted the attention of many scientists, because they not only have all of the advantages of antibodies, but also have unique merits, such as thermal stability, low cost, and unlimited applications. In this review, we present the reasons why aptamers are known as alternatives to antibodies. Furthermore, several types of in vitro selection processes, including nitrocellulose membrane filtration, affinity chromatography, magnetic bead, and capillary electrophoresis-based selection methods, are explained in detail. We also introduce various applications of aptamers for the diagnosis of diseases and detection of small molecules. Numerous analytical techniques, such as electrochemical, colorimetric, optical, and mass-sensitive methods, can be utilized to detect targets, due to convenient modifications and the stability of aptamers. Finally, several medical and analytical applications of aptamers are presented. In summary, aptamers are promising materials for diverse areas, not just as alternatives to antibodies, but as the core components of medical and analytical equipment.
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Aptâmeros de Peptídeos , Técnicas Biossensoriais , Técnica de Seleção de AptâmerosRESUMO
Incorporating functionality into the framework of metal-organic frameworks (MOFs) has attracted substantial interest because the physical and chemical properties of MOFs can be tuned by functionalizing pores. The ligand functionalization of MOF-74 is challenging because of its pristine organic ligand and framework structure. Herein, we report a series of ligand-functionalized Ni-MOF-74 derivatives synthesized by defect engineering using a mixed-ligand approach. Defect generation and ligand functionalization of Ni-MOF-74 were simultaneously achieved by incorporation of fragmented organic ligands such as 5-formylsalicylic acid, 3-hydroxysalicylic acid, 2-hydroxynicotinic acid and 5-hydroxy-1H-benzimidazole-4-carboxylic acid. The resulting defect-engineered Ni-MOF-74 derivatives maintained relatively good crystallinity up to fragment incorporation levels of â¼20% and exhibited modified permanent porosity and CO2 adsorption properties depending on the functional groups and defect concentrations in the framework.
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Since the emergence of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), various vaccines are being developed, with most vaccine candidates focusing on the viral spike protein. Here, we developed a previously unknown subunit vaccine comprising the receptor binding domain (RBD) of the spike protein fused with the tetanus toxoid epitope P2 (RBD-P2) and tested its efficacy in rodents and nonhuman primates (NHPs). We also investigated whether the SARS-CoV-2 nucleocapsid protein (N) could increase vaccine efficacy. Immunization with N and RBD-P2 (RBDP2/N) + alum increased T cell responses in mice and neutralizing antibody levels in rats compared with those obtained using RBD-P2 + alum. Furthermore, in NHPs, RBD-P2/N + alum induced slightly faster SARS-CoV-2 clearance than that induced by RBD-P2 + alum, albeit without statistical significance. Our study supports further development of RBD-P2 as a vaccine candidate against SARS-CoV-2. Also, it provides insights regarding the use of N in protein-based vaccines against SARS-CoV-2.
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Vacinas contra COVID-19/imunologia , COVID-19/prevenção & controle , Proteínas do Nucleocapsídeo de Coronavírus/imunologia , Proteínas Recombinantes de Fusão/imunologia , SARS-CoV-2/imunologia , Glicoproteína da Espícula de Coronavírus/imunologia , Toxoide Tetânico/imunologia , Animais , COVID-19/genética , COVID-19/imunologia , Vacinas contra COVID-19/genética , Chlorocebus aethiops , Proteínas do Nucleocapsídeo de Coronavírus/genética , Feminino , Macaca fascicularis , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Transgênicos , Fosfoproteínas/genética , Fosfoproteínas/imunologia , Domínios Proteicos , Ratos , Proteínas Recombinantes de Fusão/genética , SARS-CoV-2/genética , Células Sf9 , Glicoproteína da Espícula de Coronavírus/genética , Spodoptera , Toxoide Tetânico/genética , Células VeroRESUMO
It is well known that the engrailed-2 (EN2) protein, a biomarker for prostate cancer, strongly binds to a specific DNA sequence (5'-TAATTA-3') to regulate transcription. Based on this intrinsic property, DNA probes with additional flanked sequences were designed and optimized. Various measurements, such as electrophoresis mobility shift assay, surface plasmon resonance, and quantitative fluorescence assay were performed to investigate the feasibility of the DNA probes. Then, the affinities of the DNA probes to the target protein were quantitatively determined using FAM-modified DNA probes and magnetic beads, resulting in dissociation constants ranging from 61.03 to 98.84nM. To develop an early diagnosis platform for prostate cancer, an ultrasensitive electrochemical biosensor based on the electrodeposition of gold nanoparticles was designed. The EN2 protein was quantitatively detected using the electrochemical biosensor, and the calculated detection limit was found to be 5.62fM. Finally, the specificity and applicability of the biosensor were verified using several proteins and an artificial urine medium. The impedance signals increased in the cases of EN2, suggesting that the system exhibited high selectivity to only EN2.
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Sondas de DNA , Técnicas Eletroquímicas/instrumentação , Proteínas de Homeodomínio/urina , Proteínas do Tecido Nervoso/urina , Neoplasias da Próstata/diagnóstico , Neoplasias da Próstata/urina , Sequência de Bases , Sondas de DNA/química , Desenho de Equipamento , Proteínas de Homeodomínio/análise , Humanos , Limite de Detecção , Masculino , Modelos Moleculares , Proteínas do Tecido Nervoso/análiseRESUMO
Although various studies related to nanoparticles-based photothermal therapy have been actively performed, an epoch-making photothermolysis therapy exhibiting both high selectivity and efficiency has yet not been discovered. For the first time, we have developed novel valuable therapeutic complexes, namely, dual aptamer-modified gold nanostars, for the targeting of prostate cancers, including PSMA(+) and PSMA(-) cells. The synthesized probes were characterized through several techniques, including UV-VIS spectral analysis, DLS analysis, zeta potential measurements, and TEM imaging, and were subsequently subjected to cytotoxicity tests, cell uptake confirmation, and in vitro photothermal therapy. The homogeneously well-fabricated nanostars presented high selectivity to prostate cancer cells and extremely high efficiency for therapy using an 808 nm laser under an irradiance of 0.3 W cm-2, which is lower than the permitted value for skin exposure (0.329 W cm-2). It is anticipated that this novel photothermal agent will become the general platform for targeted therapy.
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A simple, sensitive, and selective colorimetric biosensor for the detection of the malarial biomarkers Plasmodium vivax lactate dehydrogenase (PvLDH) and Plasmodium falciparum LDH (PfLDH) was demonstrated using the pL1 aptamer as the recognition element and gold nanoparticles (AuNPs) as probes. The proposed method is based on the aggregation of AuNPs using hexadecyltrimethylammonium bromide (CTAB). The AuNPs exhibited a sensitive color change from red to blue, which could be seen directly with the naked eye and was monitored using UV-visible absorption spectroscopy and transmission electron microscopy (TEM). The reaction conditions were optimized to obtain the maximum color intensity. PvLDH and PfLDH were discernible with a detection limit of 1.25 pM and 2.94 pM, respectively. The applicability of the proposed biosensor was also examined in commercially available human serum.
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Aptâmeros de Nucleotídeos/química , Colorimetria/métodos , L-Lactato Desidrogenase/sangue , Malária Falciparum/sangue , Plasmodium falciparum/isolamento & purificação , Proteínas de Protozoários/sangue , Ouro/química , Humanos , Limite de Detecção , Malária Falciparum/diagnóstico , Malária Falciparum/microbiologia , Nanopartículas Metálicas/química , Nanopartículas Metálicas/ultraestrutura , Plasmodium falciparum/enzimologiaRESUMO
Finding a highly sensitive diagnostic technique for malaria has challenged scientists for the last century. In the present study, we identified versatile single-strand DNA aptamers for Plasmodium lactate dehydrogenase (pLDH), a biomarker for malaria, via the Systematic Evolution of Ligands by EXponential enrichment (SELEX). The pLDH aptamers selectively bound to the target proteins with high sensitivity (K(d)=16.8-49.6 nM). The selected aptamers were characterized using an electrophoretic mobility shift assay, a quartz crystal microbalance, a fluorescence assay, and circular dichroism spectroscopy. We also designed a simple aptasensor using electrochemical impedance spectroscopy; both Plasmodium vivax LDH and Plasmodium falciparum LDH were selectively detected with a detection limit of 1 pM. Furthermore, the pLDH aptasensor clearly distinguished between malaria-positive blood samples of two major species (P. vivax and P. falciparum) and a negative control, indicating that it may be a useful tool for the diagnosis, monitoring, and surveillance of malaria.