Complete genome of the multidrug-resistant Acinetobacter baumannii strain KBN10P02143 isolated from Korea.

Acinetobacter baumannii, a strictly aerobic, non-fermentative, Gram-negative coccobacillary rod-shaped bacterium, is an opportunistic pathogen in humans. We recently isolated a multidrug-resistant A. baumannii strain KBN10P02143 from the pus sample drawn from a surgical patient in South Korea. We report the complete genome of this strain, which consists of 4,139,396 bp (G + C content, 39.08%) with 3,868 protein-coding genes, 73 tRNAs and six rRNA operons. Identification of the genes related to multidrug resistance from this genome and the discovery of a novel conjugative plasmid will increase our understanding of the pathogenicity associated with this species.

Kinetics analysis of growth and lactic acid production in pH-controlled batch cultures of Lactobacillus casei KH-1 using yeast extract/corn steep liquor/glucose medium.

This study was performed to determine the optimal conditions of yeast extract, corn steep liquor and glucose concentration for the growth and lactic acid production of Lactobacillus casei KH-1 and to assess the effect of these conditions using a response surface methodology. A Box-Behnken design was used as an experimental design for the allocation of treatment combination as 17 pH-controlled batch cultures. The growth and product parameters were estimated by Gombertz, Leudeking and Piret models from experimental data, and analyzed statistically with response surfaces. The effects of yeast extract, corn steep liquor and glucose were significant for the maximum specific growth rate, mu(max) and the maximum biomass concentration, X(ma). The interaction of corn steep liquor and glucose indicated that the positive or negative effect of glucose on mu(max) in corn steep liquor below or above 2.1% could be explained by the glucose-dependent availability of a nutrient on mu(max) Although the experiment was achieved in pH-controlled batch culture for L. casei KH-1, the growth- and non-growth-associated production rate parameters, a and b, were significant in the response surface model. The growth and lactic acid production of L. casei KH-1 were strongly affected by glucose and the importance of the media composition was demonstrated. The estimated optimal conditions of the growth and lactic acid production of L. casei KH-1 were 1.276% and 0.697% for yeast extract, 3.505% and 1.708% for corn steep liquor, and 2.390% and 2.215% for glucose, respectively.

Complete genome of the multidrug-resistant Acinetobacter baumannii strain KBN10P02143 isolated from Korea

Acinetobacter baumannii, a strictly aerobic, non-fermentative, Gram-negative coccobacillary rod-shaped bacterium, is an opportunistic pathogen in humans. We recently isolated a multidrug-resistant A. baumannii strain KBN10P02143 from the pus sample drawn from a surgical patient in South Korea. We report the complete genome of this strain, which consists of 4,139,396 bp (G + C content, 39.08%) with 3,868 protein-coding genes, 73 tRNAs and six rRNA operons. Identification of the genes related to multidrug resistance from this genome and the discovery of a novel conjugative plasmid will increase our understanding of the pathogenicity associated with this species.