RESUMO
While prior scholarship on preventive health behaviors has focused on identifying their cognitive predictors, emerging literature suggests that emotion may also be an important determinant of health behaviors. Drawing from appraisal theory and the discrete-emotions models of affect, the current study establishes emotional pathways to support for COVID-19 policies and social distancing behavior. Analyses of survey data collected in the U.S. and South Korea demonstrate that negative emotion experienced following partisan media use increases support for COVID-19 policies and social distancing behavior. Particularly, fear and anxiety toward the pandemic emerged as strong mediators in both countries while sadness also mediated the pathways in the U.S.
Assuntos
COVID-19 , Humanos , COVID-19/epidemiologia , COVID-19/prevenção & controle , SARS-CoV-2 , Emoções , Ansiedade , MedoRESUMO
In this study, we isolated a novel avian reovirus (ARV) strain, K738/14, from a broiler chicken with viral arthritis in South Korea. Genome sequence comparisons showed relatively low nucleotide identity with previously identified ARV strains. Phylogenetic analyses suggested multiple reassortment events between reovirus strain S1133 and reoviruses of Hungarian, Chinese, and US origin had occurred. In addition, recombination analyses showed evidence of intra-segmental recombination in the M2 and S2 genes. Based on our genetic analyses, multiple reassortment events, intra-segmental recombination, and accumulation of point mutations have possibly contributed to the emergence of this novel genotype of ARV, identified in Korea.
Assuntos
Doenças das Aves/virologia , Galinhas/virologia , Genoma Viral , Orthoreovirus Aviário/genética , Orthoreovirus Aviário/isolamento & purificação , Infecções por Reoviridae/veterinária , Animais , Artrite Infecciosa/epidemiologia , Doenças das Aves/epidemiologia , Genes Virais , Genótipo , Fases de Leitura Aberta , Orthoreovirus Aviário/classificação , Filogenia , Mutação Puntual , Vírus Reordenados/genética , Recombinação Genética , Infecções por Reoviridae/virologia , República da Coreia , Análise de Sequência de DNARESUMO
Infectious bronchitis virus (IBV) infections cause great economic losses to the poultry industry worldwide. IBVs continuously evolve by developing mutations in antigenic sites; therefore, an IBV vaccine that provides broad cross-protection can be a highly relevant and practical method in IBV control strategies. Although some IBV vaccine strains are known to provide protection against multiple IBV serotypes, in general commercially available IBV vaccine strains provide protection against antigenically related viruses but not distinct heterologous viruses. In the present study we characterized the Korean variant IBV K40/09 strain with regard to its immunogenicity and protective efficacy against seven currently circulating IBV serotypes. Three-week-old specific-pathogen-free chickens were intraocularly immunized with the IBV K40/09 strain at 10(3.5) 50% egg infective dose (EID50). Three weeks after immunization all the birds were challenged with seven different strains at 10(4.5) EID50. Chickens immunized with the IBV K40/09 strain showed significantly high levels of protection against all challenge viruses at the trachea and kidney levels. Our results suggest that IBV K40/09 could be useful to ensure IBV vaccine effectiveness owing to its cross-protective ability. Therefore, the IBV K40/09 strain merits consideration as a vaccine candidate to prevent infection as well as the spread of new IBV strains and many IBV variants that have been reported worldwide.
Assuntos
Galinhas , Infecções por Coronavirus/veterinária , Vírus da Bronquite Infecciosa/imunologia , Vírus da Bronquite Infecciosa/patogenicidade , Doenças das Aves Domésticas/imunologia , Vacinas Virais/imunologia , Animais , Anticorpos Neutralizantes/sangue , Anticorpos Antivirais/sangue , Embrião de Galinha , Infecções por Coronavirus/imunologia , Infecções por Coronavirus/virologia , Proteção Cruzada , Vírus da Bronquite Infecciosa/genética , Rim/patologia , Dados de Sequência Molecular , Doenças das Aves Domésticas/virologia , República da Coreia , Análise de Sequência de DNA/veterinária , Organismos Livres de Patógenos Específicos , Traqueia/patologia , Vacinas Atenuadas/imunologia , VirulênciaRESUMO
A Salmonella Gallinarum (SG)-specific bacteriophage isolated from sewage effluent was used to prevent horizontal transmission of SG in commercial layer chickens. Six-week-old chickens, each challenged with 5 x 10(8) colony-forming units of SG, cohabited with contact chickens treated with 10(6) plaque-forming units/kg of bacteriophage, prepared in feed additives, for 7 days before, and 21 days after challenge with SG. Mortality was observed for 3 wk after challenge and SG was periodically reisolated from the liver, spleen, and cecum of chickens. SG re-isolation from organs was decreased and a significant (P < 0.05) reduction in mortality was observed in contact chickens treated with the bacteriophage, as compared to untreated contact chickens, indicating that bacteriophage administration in feed additives significantly prevented the horizontal transmission of SG. These results provide important insights into prevention and control strategies against SG infection and suggest that the use of bacteriophages may be a novel, safe, and effectively plausible alternative to antibiotics for the prevention of SG infection in poultry.
Assuntos
Galinhas , Doenças das Aves Domésticas/prevenção & controle , Doenças das Aves Domésticas/terapia , Salmonelose Animal/prevenção & controle , Salmonelose Animal/terapia , Fagos de Salmonella/fisiologia , Salmonella/virologia , Ração Animal , Animais , Dieta/veterinária , Feminino , Doenças das Aves Domésticas/microbiologia , Doenças das Aves Domésticas/mortalidade , Salmonelose Animal/microbiologia , Salmonelose Animal/mortalidadeRESUMO
Since 2007, 55 adenovirus strains have been isolated from commercial chicken flocks in Korea and have been identified and the pathogenicity of these isolates was confirmed in specific-pathogen-free chickens of different age. Based on sequencing analysis of the hexon gene, 55 FAdV isolates were genetically related to the IBH-2A strain of FAdV3 (4 isolates, 99.2% to 100%), the KR5 strain of FAdV4 (22 isolates, 97.9% to 99.2%), the 764 strain of FAdV9 (11 isolates, 99.1% to 99.3%), and the 1047 strain of FAdV11 (18 isolates, > 99%). Experimental infections with four serotypes of FAdV resulted in high mortality of 18-day-old chicken embryos and 1-day-old chicks with marked liver necrosis similar to those observed in the natural outbreaks. Notably, specific hydropericardium was observed in chicks challenged with the K531 strain (serotype 4). However, 3-wk-old chickens challenged with FAdVs, regardless of serotype, did not show any clinical signs or mortality except histologic lesions of focal hepatocytic necrosis with mild lymphocytic infiltration. The results indicate that four FAdV serotypes (3, 4, 9, and 11) are the dominant serotypes of FAdVs in the Korea and are pathogenic enough to cause clinical disease in young chicks. The present investigation provides important information on the epidemiology and pathogenesis of FAdVs and highlights the importance of control strategies against FAdV infection in Korea.
Assuntos
Infecções por Adenoviridae/veterinária , Aviadenovirus/patogenicidade , Doenças das Aves Domésticas/virologia , Infecções por Adenoviridae/epidemiologia , Infecções por Adenoviridae/virologia , Animais , Aviadenovirus/isolamento & purificação , Galinhas , Óvulo/virologia , Filogenia , Doenças das Aves Domésticas/epidemiologia , República da Coreia/epidemiologia , Organismos Livres de Patógenos Específicos , VirulênciaRESUMO
An outbreak of avian influenza, caused by an H9N2 low-pathogenic avian influenza virus (AIV), occurred in a chicken farm and caused severe economic losses due to mortality and diarrhea. AIV was isolated and identified in a sample from an affected native Korean chicken. Genetic analysis of the isolate revealed a high sequence similarity to genes of novel reassortant H9N2 viruses isolated from slaughterhouses and live bird markets in Korea in 2008 and 2009. Animal challenge studies demonstrated that the replication kinetics and pathogenicity of the isolate were considerably altered due to adaptation in chickens. Vaccine protection studies indicated that commercial vaccine was not able to prevent virus shedding and clinical disease when chickens were challenged with the isolate. These results suggest that the novel H9N2 virus possesses the capacity to replicate efficiently in the respiratory system against vaccination and to cause severe disease in domestic chickens. The results also highlight the importance of appropriate updating of vaccine strains, based on continuous surveillance data, to prevent the possibility of a new H9N2 epidemic in Korea.
Assuntos
Galinhas , Vírus da Influenza A Subtipo H9N2/isolamento & purificação , Influenza Aviária/virologia , Animais , Surtos de Doenças/veterinária , Vírus da Influenza A Subtipo H9N2/genética , Vacinas contra Influenza/imunologia , Influenza Aviária/epidemiologia , Influenza Aviária/prevenção & controle , República da Coreia/epidemiologiaRESUMO
In recent years, myoelectric interfaces using surface electromyogram (EMG) signals have been developed for assisting people with physical disabilities. Especially, in the myoelectric interfaces for robotic hands or arms, decoding the user's upper-limb movement intentions is cardinal to properly control the prosthesis. However, because previous experiments were implemented with only healthy subjects, the possibility of classifying reaching-to-grasping based on the EMG signals from the residual limb without the below-elbow muscles was not investigated yet. Therefore, we aimed to investigate the possibility of classifying reaching-to-grasping tasks using the EMG from the upper arm and upper body without considering wrist muscles for prosthetic users. In our study, seven healthy subjects, one trans-radial amputee, and one wrist amputee were participated and performed 10 repeatable 12 reaching-to-grasping tasks based on the Southampton Hand Assessment Procedure (SHAP) with 12 different weighted (light and heavy) objects. The acquired EMG was processed using the principal component analysis (PCA) and convolutional neural network (CNN) to decode the tasks. The PCA-CNN method showed that the average accuracies of the healthy subjects were 69.4 ± 11.4%, using only the EMG signals by the upper arm and upper body. The result with the PCA-CNN method showed 8% significantly higher accuracies than the result with the widely used time domain and auto-regressive-support vector machine (TDAR-SVM) method as 61.6 ± 13.7%. However, in the cases of the amputees, the PCA-CNN showed slightly lower performance. In addition, in the aspects of assistant daily living, because grip force is also important when grasping an object after reaching, the possibility of classifying the two light and heavy objects in each reaching-to-grasping task was also investigated. Consequently, the PCA-CNN method showed higher accuracy at 70.1 ± 9.8%. Based on our results, the PCA-CNN method can help to improve the performance of classifying reaching-to-grasping tasks without wrist EMG signals. Our findings and decoding method can be implemented to further develop a practical human-machine interface using EMG signals.
RESUMO
Compartment syndrome (CS) is a pathological event caused by elevated intracompartmental pressure (ICP); however, changes from the onset of inducing atraumatic CS remained unclear. The study aimed to investigate the physiological changes in a newly developed in vivo porcine acute atraumatic CS model. CS was induced by ischemia-reperfusion injury in the left hind leg of fourteen pigs divided into an echogenicity group (EG) and a shear wave elastography group (SEG). Echogenicity was measured in EG, and shear elastic modulus (SEM) was measured in SEG seven times before, at the onset of inducing CS, and every 30 min after the onset over eight hours. Simultaneously, ICP, blood pressure, and muscle perfusion pressure (MPP) were also measured in both groups. Our results indicate that SEM of the experimental leg in SEG significantly increased as CS developed compared to the control leg (p = 0.027), but no statistical difference in the echogenicity in EG was found between the experimental leg and control leg. There were also significant correlations between SEM and ICP (p < 0.001) and ICP and MPP (p < 0.001). Our method and findings can be a basis to develop a non-invasive diagnostic tool using a shear wave elastography for atraumatic CS.
Assuntos
Síndromes Compartimentais/diagnóstico por imagem , Técnicas de Imagem por Elasticidade/métodos , Animais , Síndromes Compartimentais/etiologia , Síndromes Compartimentais/fisiopatologia , Modelos Animais de Doenças , Módulo de Elasticidade/fisiologia , Extremidades/diagnóstico por imagem , Humanos , Masculino , Sus scrofaRESUMO
Cronobacter sakazakii is an opportunistic pathogenic bacterium found in powdered infant formula and is fatal to neonates. Antibiotic resistance has emerged owing to overuse of antibiotics. Therefore, demand for high-yield bacteriophages as an alternative to antibiotics has increased. Accordingly, we developed a modified mass-production method for bacteriophages by introducing a two-stage self-cycling (TSSC) process, which yielded high-concentration bacteriophage solutions by replenishing the nutritional medium at the beginning of each process, without additional challenge. pH of the culture medium was monitored in real-time during C. sakazakii growth and bacteriophage CS01 propagation, and the changes in various parameters were assessed. The pH of the culture medium dropped to 5.8 when the host bacteria reached the early log phase (OD540 = 0.3). After challenge, it decreased to 4.65 and then recovered to 4.94; therefore, we set the optimum pH to challenge the phage at 5.8 and that to harvest the phage at 4.94. We then compared phage production during the TSSC process in jar-type bioreactors and the batch culture process in shaker flasks. In the same volume of LB medium, the concentration of the phage titer solution obtained with the TSSC process was 24 times higher than that obtained with the batch culture process. Moreover, we stably obtained high concentrations of bacteriophage solutions for three cycles with the TSSC process. Overall, this modified TSSC process could simplify large-scale production of bacteriophage CS01 and reduce the unit cost of phage titer solution. These results could contribute to curing infants infected with antibiotic-resistant C. sakazakii.
Assuntos
Bacteriófagos/crescimento & desenvolvimento , Reatores Biológicos , Cronobacter sakazakii/virologia , Cultura de Vírus/métodos , Meios de Cultura , Microbiologia de Alimentos , Fórmulas Infantis/microbiologiaRESUMO
Cronobacter sakazakii is an opportunistic pathogen causing serious infections in neonates. In this study, a bacteriophage ΦCS01, which infects C. sakazakii, was isolated from swine feces and its morphology, growth parameters, and genomic analysis were investigated. Transmission electron microscopy revealed that ΦCS01 has a spherical head and is 65.74 nm in diameter with a 98.75 nm contracted tail, suggesting that it belongs to the family Myoviridae. The major viral proteins are approximately 71 kDa and 64 kDa in size. The latent period of ΦCS01 was shown to be 60 min, and the burst size was 90.7 pfu (plaque-forming units)/ infected cell. Bacteriophage ΦCS01was stable at 4-60°C for 1 h and lost infectivity after 1 h of heating at 70°C. Infectivity remained unaffected at pH 4-9 for 2 h, while the bacteriophage was inactivated at pH <3 or >10. The double-stranded ΦCS01 DNA genome consists of 48,195 base pairs, with 75 predicted open reading frames. Phylogenetic analysis is closely related to that of the previously reported C. sakazakii phage ESP2949-1. The newly isolated ΦCS01 shows infectivity in the host bacterium C. sakazakii, indicating that it may be a promising alternative to antibacterial agents for the removal of C. sakazakii from powdered infant formulas.
Assuntos
Bacteriófagos/classificação , Bacteriófagos/genética , Bacteriófagos/fisiologia , Cronobacter sakazakii/virologia , Genoma Viral , Filogenia , Animais , Bacteriófagos/isolamento & purificação , DNA , Fezes/microbiologia , Inocuidade dos Alimentos , Especificidade de Hospedeiro , Concentração de Íons de Hidrogênio , Myoviridae/classificação , Fases de Leitura Aberta , Suínos , Temperatura , Proteínas Virais/química , Sequenciamento Completo do GenomaRESUMO
A natural recombinant nephropathogenic K40/09 strain of infectious bronchitis virus (IBV) was heat-adapted for possible future use as live attenuated vaccine. The K40/09 strain was selected during successive serial passages in specific-pathogen free (SPF) embryonated eggs at sub-optimal higher temperature (56°C). Unlike the parental strain, the attenuated strain, designated K40/09 HP50, was found to be safe in 1-day-old SPF chicks, which showed neither mortality nor signs of morbidity, and rarely induced ciliostasis or histological changes in the trachea and kidney after intraocular and fine-spray administration. K40/09 HP50 provided almost complete protection against two distinct subgroups of a nephropathogenic strain (KM91-like and QX-like subgroup) and elicited the production of high titers of neutralizing antibody (neutralization index of 3.6). We conclude that the K40/09 HP50 vaccine virus is rapidly attenuated by heat adaptation and exhibits the desired level of attenuation, immunogenicity, and protective efficacy required for a live attenuated vaccine. These results indicate that the K40/09 vaccine could be helpful for the reduction of economic losses caused by recently emergent nephropathogenic IBV infection in many countries.
Assuntos
Adaptação Biológica , Infecções por Coronavirus/veterinária , Vírus da Bronquite Infecciosa/imunologia , Doenças das Aves Domésticas/prevenção & controle , Vacinas Virais/imunologia , Animais , Galinhas , Infecções por Coronavirus/prevenção & controle , Temperatura Alta , Vírus da Bronquite Infecciosa/isolamento & purificação , Rim/patologia , Doenças das Aves Domésticas/imunologia , Inoculações Seriadas , Análise de Sobrevida , Traqueia/patologia , Vacinas Atenuadas/administração & dosagem , Vacinas Atenuadas/efeitos adversos , Vacinas Atenuadas/imunologia , Vacinas Atenuadas/isolamento & purificação , Vacinas Virais/administração & dosagem , Vacinas Virais/efeitos adversos , Vacinas Virais/isolamento & purificaçãoRESUMO
The number of clinical cases of inclusion body hepatitis (IBH) and hydropericardium-hepatitis syndrome (HHS) has been increasing, resulting in considerable economic losses in many countries. Currently, only fowl Adenovirus (FAdV) serotype 4 (FAdV-4) has been reported as the causative agent of HHS, whereas IBH can be caused by all 12 serotypes of FAdV. For protection against HHS, various live and killed FAdV serotype 4 vaccines have been developed. However, there is a concern whether these vaccines composed of FAdV-4 alone could provide protection against IBH, which is caused by other serotypes of virulent FAdVs. To date, there have been no reports evaluating the protective efficacy of the FAdV-4 vaccine against other serotypes of FAdV. Thus, we investigated the cross-protection efficacy of an inactivated oil-emulsion FAdV-4 vaccine against various serotypes of FAdV field isolates. Our study demonstrated that the inactivated oil-emulsion FAdV-4 vaccine could provide broad cross-protection against various serotypes of FAdV in not only vaccinated birds, but also the progenies of vaccinated breeder. Therefore, we conclude that the inactivated oil-emulsion FAdV-4 vaccine could be effective in preventing the spread of various other serotypes of FAdV as well as FAdV-4 infection in the poultry industry.
Assuntos
Infecções por Adenoviridae/prevenção & controle , Proteção Cruzada , Doenças das Aves Domésticas/prevenção & controle , Vacinas Virais/imunologia , Animais , Anticorpos Antivirais/sangue , Formação de Anticorpos , Aviadenovirus/classificação , Galinhas , Hepatite Viral Animal/prevenção & controleRESUMO
A reduced graphene oxide (GO) layer was produced on undoped and n-type GaN, and its effect on the current- and heat-spreading properties of GaN-based light-emitting diodes (LEDs) was studied. The reduced GO inserted between metal electrode and GaN semiconductor acted as a conducting layer and enhanced lateral current flow in the device. Especially, introduction of the reduced GO layer on the n-type GaN improved the electrical performance of the device, relative to that of conventional LEDs, due to a decrease in the series resistance of the device. The enhanced current-spreading was further of benefit, giving the device a higher light output power and a lower junction temperature at high injection currents. These results therefore indicate that reduced GO can be a suitable current and heat-spreading layer for GaN-based LEDs.
RESUMO
BACKGROUND: Currently, Asian lineage highly pathogenic avian influenza (HPAI) H5N1 has become widespread across continents. These viruses are persistently circulating among poultry populations in endemic regions, causing huge economic losses, and raising concerns about an H5N1 pandemic. To control HPAI H5N1, effective vaccines for poultry are urgently needed. OBJECTIVE: In this study, we developed HPAI virus-like particle (VLP) vaccine as a candidate poultry vaccine and evaluated its protective efficacy and possible application for differentiating infected from vaccinated animals (DIVA). METHODS: Specific pathogen-free chickens received a single injection of HPAI H5N1 VLP vaccine generated using baculovirus expression vector system. Immunogenicity of VLP vaccines was determined using hemagglutination inhibition (HI), neuraminidase inhibition (NI), and ELISA test. Challenge study was performed to evaluate efficacy of VLP vaccines. RESULTS AND CONCLUSIONS: A single immunization with HPAI H5N1 VLP vaccine induced high levels of HI and NI antibodies and protected chickens from a lethal challenge of wild-type HPAI H5N1 virus. Viral excretion from the vaccinated and challenged group was strongly reduced compared with a mock-vaccinated control group. Furthermore, we were able to differentiate VLP-vaccinated chickens from vaccinated and then infected chickens with a commercial ELISA test kit, which offers a promising strategy for the application of DIVA concept.
Assuntos
Virus da Influenza A Subtipo H5N1/imunologia , Vacinas contra Influenza/imunologia , Influenza Aviária/prevenção & controle , Doenças das Aves Domésticas/prevenção & controle , Vacinas de Partículas Semelhantes a Vírus/imunologia , Animais , Anticorpos Antivirais/imunologia , Galinhas , Virus da Influenza A Subtipo H5N1/patogenicidade , Vacinas contra Influenza/administração & dosagem , Vacinas contra Influenza/genética , Influenza Aviária/imunologia , Influenza Aviária/virologia , Doenças das Aves Domésticas/imunologia , Doenças das Aves Domésticas/virologia , Organismos Livres de Patógenos Específicos , Vacinação , Vacinas de Partículas Semelhantes a Vírus/administração & dosagem , Vacinas de Partículas Semelhantes a Vírus/genéticaRESUMO
Bacteriophage ΦCJ07 with broad host ranges for Salmonella strains isolated from sewage effluent were used to reduce Salmonella Enteritidis (SE) infection in chickens. One-day-old chicks challenged with 5×10(7) colony-forming units/bird of SE were cohabitated with contact chicks and treated with three concentrations (10(5), 10(7) and 10(9) plaque forming units (PFU)/g) of bacteriophage prepared as a feed additive for 21days after challenge. Salmonella in the intestine was quantified and environmental contamination level was examined at 1, 2 and 3weeks after challenge. All treatments reduced intestinal SE colonization in challenged and contact chickens and reduced the environmental contamination level, but the reductions produced by 10(7) and 10(9)PFU/g of bacteriophage were significant (P<0.05) as compared with untreated controls. In addition, seven out of 10 (70%) contact chickens treated with 10(9)PFU/g of bacteriophage had no detectable intestinal Salmonella at 3weeks after treatment, suggesting that bacteriophage therapy significantly prevented the horizontal transmission of SE. These results provide important insights into preventive and control strategies against SE infection in poultry and indicate that the use of bacteriophage could reduce the incidence of Salmonella food poisoning.
Assuntos
Bacteriófagos/fisiologia , Galinhas , Doenças das Aves Domésticas/prevenção & controle , Salmonelose Animal/prevenção & controle , Salmonella enteritidis/virologia , Animais , Controle Biológico de Vetores , Doenças das Aves Domésticas/microbiologia , Salmonelose Animal/microbiologiaRESUMO
The infectious bronchitis virus (IBV) is continuously evolving through point mutation and recombination of their genome, subsequently the emergence of IBV variants complicates disease control. The objective of this study was to investigate genetic characterization of new IBV variants isolated from commercial chicken flocks in Korea collected between 2005 and 2010. Phylogenetic analysis revealed that all new IBV isolates belonged to Korean group II (K-II), which included the nephropathogenic IBV strains. However, the isolates formed a new gene cluster that was distinguished from the two distinct K-II subgroups (KM91-like and QX-like). Recombination events were identified in the S1 gene, with their putative parental strains being the KM91-like or QX-like subgroup. In addition, two crossover sites were observed in the S1 gene of IBV isolates. These results suggest that natural genetic recombination between heterologous strains classified into different genetic groups has occurred and may have caused the emergence of new IBV strains. This finding provides important information on IBV evolution and is essential for the effective control of IB in Korea.
Assuntos
Galinhas , Infecções por Coronavirus/veterinária , Vírus da Bronquite Infecciosa/genética , Doenças das Aves Domésticas/virologia , Sequência de Aminoácidos , Animais , Infecções por Coronavirus/epidemiologia , Infecções por Coronavirus/virologia , Vírus da Bronquite Infecciosa/classificação , Funções Verossimilhança , Modelos Genéticos , Dados de Sequência Molecular , Filogenia , Doenças das Aves Domésticas/epidemiologia , Recombinação Genética , República da Coreia/epidemiologia , Alinhamento de Sequência , Análise de Sequência de DNA , Proteínas Virais de Fusão/genéticaRESUMO
Avian influenza viruses (AIV) can be genetically distinguished by geographical origin. The present study found evidence of intercontinental transfer of North American lineage AIV into Asia via migratory bird populations. The North American lineage genes were detected in live animal markets during avian influenza surveillance, seemed to have reassorted with Eurasian AIV in wild bird habitats, and had transmitted to live animal markets. Enhanced AIV surveillance is required to understand the influence of newly transferred North American lineage AIV genes on AIV evolution in Asia and to investigate AIV ecology in various transcontinental migrant species.
Assuntos
Aves/virologia , Influenza Aviária/epidemiologia , Animais , Embrião de Galinha , Genes Virais , Vírus da Influenza A/genética , Vírus da Influenza A/isolamento & purificação , Influenza Aviária/virologia , América do Norte , República da Coreia/epidemiologia , Reação em Cadeia da Polimerase Via Transcriptase ReversaRESUMO
Highly pathogenic avian influenza (HPAI) H5N1 virus circulates among a variety of free-ranging wild birds and continually poses a threat to animal and human health. During the winter of 2010-2011, we surveyed Korean wild bird habitats. From 728 fresh fecal samples, 14 HPAI H5N1 viruses were identified. The isolates phylogenetically clustered with other recently isolated clade 2.3.2 HPAI H5N1 viruses isolated from wild birds in Mongolia. All HPAI-positive fecal samples were analyzed by DNA barcoding for host-species identification. Twelve of the 14 HPAI-positive samples were typed as Mandarin Duck (Aix galericulata). The high incidence of HPAI subtype H5N1 viruses in wild Mandarin Duck droppings is a novel finding and underscores the need for enhanced avian influenza virus surveillance in wild Mandarin Ducks. Further investigation of the susceptibility of Mandarin Ducks to HPAI H5N1 clade 2.3.2 virus would aid the understanding of HPAI ecology and epidemiology in wild birds.