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1.
Clin Exp Immunol ; 179(2): 161-72, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25351931

RESUMO

One would assume that the anti-inflammatory activity of α1-anti-trypsin (AAT) is the result of inhibiting neutrophil enzymes. However, AAT exhibits tolerogenic activities that are difficult to explain by serine-protease inhibition or by reduced inflammatory parameters. Targets outside the serine-protease family have been identified, supporting the notion that elastase inhibition, the only functional factory release criteria for clinical-grade AAT, is over-emphasized. Non-obvious developments in the understanding of AAT biology disqualify it from being a straightforward anti-inflammatory agent: AAT does not block dendritic cell activities, nor does it promote viral and tumour susceptibilities, stunt B lymphocyte responses or render treated patients susceptible to infections; accordingly, outcomes of elevated AAT do not overlap those attained by immunosuppression. Aside from the acute-phase response, AAT rises during the third trimester of pregnancy and also in advanced age. At the molecular level, AAT docks onto cholesterol-rich lipid-rafts and circulating lipid particles, directly binds interleukin (IL)-8, ADAM metallopeptidase domain 17 (ADAM17) and danger-associated molecular pattern (DAMP) molecules, and its activity is lost to smoke, high glucose levels and bacterial proteases, introducing a novel entity - 'relative AAT deficiency'. Unlike immunosuppression, AAT appears to help the immune system to distinguish between desired responses against authentic threats, and unwanted responses fuelled by a positive feedback loop perpetuated by, and at the expense of, inflamed injured innocent bystander cells. With a remarkable clinical safety record, AAT treatment is currently tested in clinical trials for its potential benefit in a variety of categorically distinct pathologies that share at least one common driving force: cell injury.


Assuntos
Imunidade Adaptativa , Linfócitos B/imunologia , Células Dendríticas/imunologia , Imunidade Inata , Terapia de Imunossupressão/métodos , alfa 1-Antitripsina/uso terapêutico , Proteínas ADAM/antagonistas & inibidores , Proteínas ADAM/imunologia , Proteína ADAM17 , Linfócitos B/patologia , Células Dendríticas/patologia , Feminino , Humanos , Tolerância Imunológica/efeitos dos fármacos , Gravidez , alfa 1-Antitripsina/imunologia
2.
Braz J Biol ; 83: e270865, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37222368

RESUMO

The present study aimed to evaluate concentrations and forms of application of salicylic acid used for water stress mitigation on the gas exchange and growth of yellow passion fruit. The experimental design was arranged in randomized blocks in a 4 × 4 × 2 factorial scheme, with four concentrations of salicylic acid (SA) via foliar application (0.0, 0.7, 1.4, and 2.1 mM), four SA concentrations via fertigation (0.0, 0.7, 1.4, and 2.1 mM), and two irrigation depths estimated based on the actual evapotranspiration - ETr (50 and 100% of ETr), with three replications. Water stress negatively affected the physiology and growth of yellow passion fruit seedlings at 75 days after sowing (DAS). The application of salicylic acid, regardless of the form of application, attenuates the effects of water stress on gas exchange and growth of yellow passion fruit, with the best results obtained when applying a concentration of 1.30 mM via leaf or 0.90 mM via fertirrigation. The combination of foliar application of AS and fertigation contributed to improve photosynthetic and growth parameters under water conditions of 50 and 100% of ETr. The foliar application of AS presents superior responses to the application via fertigation. These results reinforce the hypothesis that the attenuation of water stress by salicylic acid is related to the maintenance of gas exchange, which depends on the concentration and form of application, and studies testing combinations throughout the crop cycle become promising for advances in knowledge from the action of this phytohormone on abiotic stress.


Assuntos
Passiflora , Desidratação , Frutas , Plântula , Ácido Salicílico
3.
Braz J Biol ; 83: e272615, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37222374

RESUMO

The limitation in the quality of water sources for irrigation in the semi-arid region of northeastern Brazil is increasingly present, so it is necessary to use water with high concentrations of salts for agricultural production, which makes the use of elicitors essential to mitigate the harmful effects of salinity on plants. Given the above, the objective of this study was to evaluate the effects of foliar application of salicylic acid on the mineral composition and production of guava plants under salt stress conditions in the post-grafting phase. The experiment was carried out under greenhouse conditions, in a randomized block design, in a 2 × 4 factorial scheme, with two levels of electrical conductivity of irrigation water (0.6 and 3.2 dS m-1) and four concentrations of salicylic acid (0, 1.2, 2.4, and 3.6 mM), with three replicates. During the flowering stage of guava, N, P, and K contents accumulated in the leaves according to the following order of concentration: N > K > P. Foliar application of 1.2 mM of salicylic acid increases the leaf contents of N, P, and K in guava plants grown under irrigation with water of 0.6 dS m-1. Water salinity of 3.2 dS m-1 reduces the growth and production components of guava plants.


Assuntos
Psidium , Ácido Salicílico , Estresse Salino , Minerais , Água
4.
Braz J Biol ; 83: e274595, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37792747

RESUMO

The objective of this study was to evaluate the salicylic acid applications in attenuating the harmful effects of saline nutrient solution on the physiology and growth of 'Gaúcho' melon cultivated in the NFT hydroponic system. The experiment was conducted in a greenhouse, in Pombal-PB, Brazil. The cultivation system used was the Nutrient Film Technique - NFT hydroponics. A completely randomized split-plot design was used, with the plot referring to four levels of salinity in the nutrient solution - ECns (2.1 control, 3.2, 4.3, and 5.4 dS m-1) and the sub-plot four concentrations of salicylic acid (SA) (0, 1.5, 3.0, and 4.5 mM), applied via foliar spray, with six replications. Nutrient solution of 4.3 and 5.4 dS m-1 electrical conductivity promotes higher maximum and variable fluorescence, respectively. The stomatal conductance, transpiration, stem diameter, main branch length, leaf dry mass, and stem dry mass of 'Gaúcho' melon plants decrease with the increase in salinity of the nutrient solution. Salicylic acid increases the initial fluorescence and the main branch length of 'Gaúcho' melon plants in hydroponic cultivation. Salicylic acid at a concentration of 1.5 to 4.5 mM did not attenuate the effects of salt stress on the internal CO2 concentration, CO2 assimilation rate, and root dry mass of 'Gaúcho' melon plants.


Assuntos
Dióxido de Carbono , Cucurbitaceae , Hidroponia , Ácido Salicílico/farmacologia , Fluorescência , Estresse Salino , Clorofila
5.
Braz J Biol ; 83: e273017, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37255174

RESUMO

Considering the relevance of bell pepper and the limitations imposed by the deleterious effects of salt stress, especially in semi-arid regions, it is extremely important to establish strategies that can facilitate the use of saline water in vegetable production. In this scenario, this study aimed to evaluate the effect of the frequency of foliar application of salicylic acid on the morphophysiology and production of the bell pepper cv. "All Big" irrigated with saline water. The study was conducted at a greenhouse in Campina Grande - PB. The treatments were distributed in a completely randomized design and set up in a 4 × 4 factorial arrangement with three replications, corresponding to four application frequencies of salicylic acid (F1- No application of salicylic acid, F2 - Weekly application, F3- fortnightly application, and F4- monthly application) and four levels of electrical conductivity of irrigation water - ECw (0.8, 1.6, 2.4 and 3.2 dS m-1). The fortnightly application of salicylic acid at a concentration of 1.0 mM mitigated the effects of salt stress on the morphophysiology and production components of bell pepper cv. All Big cultivated with ECw of up to 2.4 dS m-1, which reinforces the hypothesis that salicylic acid can act as a signaling molecule and reduce the effects of saline stress in bell pepper, enabling the use of brackish water in agricultural activity, mainly in semi-arid regions of northeastern Brazil, which have a shortage of fresh water.


Assuntos
Capsicum , Ácido Salicílico , Ácido Salicílico/farmacologia , Estresse Salino , Agricultura , Verduras
6.
Braz J Biol ; 83: e273404, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37255204

RESUMO

The objective of this study was to evaluate the physiology and production of sugar-apple as a function of irrigation intervals and foliar application of proline under the conditions of Paraíba's semi-arid region. A randomized block design was laid out in a 4 × 2 factorial scheme, with treatments resulting from the combination of four irrigation intervals (1, 4, 8 and 12 days) and two concentrations of proline (0 and 10 mM), with four replicates, and the plot consisted of four usable plants. Increase in irrigation intervals reduced the gas exchange of sugar-apple plants at 298 days after transplanting. Exogenous application of proline at concentration of 10 mM increased contents of chlorophyll a, chlorophyll b, total chlorophyll and carotenoids and fruit fresh mass in plants grown under 12-day irrigation intervals.


Assuntos
Desidratação , Malus , Clorofila A , Prolina , Açúcares
7.
Braz J Biol ; 83: e274991, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37909589

RESUMO

The objective of this study was to evaluate the water status, photosynthetic pigments, and photochemical efficiency of mini watermelon plants under salt stress and phosphate fertilization. The experiment was conducted in pots under greenhouse conditions in Pombal, PB, Brazil. The experimental design used was randomized blocks in a 5 × 4 factorial scheme, with five levels of electrical conductivity of irrigation water - ECw (0.3, 1.3, 2.3, 3.3, and 4.3 dS m-1) and four doses of phosphorus (60, 80, 100, and 120% of the recommendation), with three replicates. The relative water content in the tissues decreased with the increase in ECw levels in all phosphorus doses, with decreases of 7.05, 7.81 and 8.83% per unit increase in ECw, in plants fertilized with 80, 100 and 120% P2O5. On the other hand, ECw levels increased electrolyte leakage, regardless of phosphorus doses of the recommendation. The synthesis of photosynthetic pigments and the quantum efficiency of photosystem II were inhibited by increasing water salinity from 0.3 dS m-1 in plants grown under phosphorus doses above 60% of the recommendation. Water salinity from 0.3 dS m-1 reduced chlorophyll b contents, initial, maximum, and variable fluorescence of mini watermelon plants, with a decrease of 11.86, 4.51, 4.53, and 4.54% per unit increment of ECw, respectively.


Assuntos
Citrullus , Fosfatos , Fotossíntese , Águas Salinas , Fósforo
8.
Braz J Biol ; 83: e275322, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37729213

RESUMO

Several studies have been conducted evaluating the management of fertilizers as strategies to mitigate the deleterious effects of salt stress, so the objective of this work was to evaluate the quality of 'Crioula' guava seedlings under irrigation with water of different salinity levels and fertilized with combinations of nitrogen and potassium. The experiment was performed in a protected (screened) environment using a randomized block design and analyzed in a 5 × 4 factorial scheme, with four replicates, with the plot formed by two plants. The treatments were formed from the combination of the factor electrical conductivity of irrigation water (ECw 0.3, 1.1, 1.9, 2.7 and 3.5 dS m-1), with the factor combinations (C) of nitrogen (N) and potassium (K2O) doses, being C1 = 70% N + 50% K2O, C2 = 100% N + 75% K2O, C3= 130% N + 100% K2O and C4= 160% N + 125% K2O. The recommended dose of 100% N and K, respectively, was 541.1 mg N dm-3 soil and 798.6 mg K dm-3 soil. The combinations of N and K fertilization corresponding to 70% N + 50% K2O and 100% N + 75% K2O of the recommended doses promoted greater growth of 'Crioula' guava seedlings. Water with ECw of 2.1 dS m-1 promotes the formation of quality 'Crioula' guava seedlings.


Assuntos
Psidium , Plântula , Salinidade , Nitrogênio , Potássio , Solo , Água
9.
Braz J Biol ; 82: e265069, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36327399

RESUMO

Salt stress caused by excess salts present in irrigation water, is one of the biggest barriers in agricultural production, especially in semi-arid regions. Thus, the use of substances, such as salicylic acid, that minimize the deleterious effects of salinity on plants can be an alternative to ensure satisfactory production. In this context, the objective of this study was to evaluate the effects of different methods of application of salicylic acid on the growth, production and water use efficiency of cherry tomato plants under salt stress. The study was conducted in a greenhouse, using an Entisol soil with a sandy loam texture. The treatments were distributed in a completely randomized design, in a 2×4 factorial arrangement, corresponding to two levels of electrical conductivity of irrigation water - ECw (0.6 and 2.6 dS m-1) and four methods of application of salicylic acid (Control - without application of SA; via spraying; via irrigation and via spraying and irrigation), with five replicates and one plant per plot. The salicylic acid concentration used in the different methods was 1.0 mM. Application of salicylic acid via foliar spraying increased the growth, production and water use efficiency of cherry tomato plants. The salt stress induced by the electrical conductivity of 2.6 dS m-1 was attenuated by the foliar application of salicylic acid. The use of water of 2.6 dS m-1 associated with the application of salicylic acid via irrigation water further intensified the adverse effects of salinity on cherry tomato plants.


Assuntos
Solanum lycopersicum , Ácido Salicílico/farmacologia , Salinidade , Estresse Salino , Água
10.
Braz J Biol ; 84: e261211, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35792744

RESUMO

To mitigate the deleterious effects of salt stress, substances capable of acting as mitigators and/or inducers of tolerance to stress have been used, enabling the use of saline waters and contributing to the development of irrigated agriculture. In this context, the aim of the present study was to evaluate the effect of foliar spraying with hydrogen peroxide as an attenuator of salt stress effects on soursop morphophysiology. The experiment was conducted under greenhouse conditions in Campina Grande - PB, Brazil, using a randomized block design, in a 4 × 4 factorial arrangement, whose treatments resulted from the combination of four levels of electrical conductivity of irrigation water - ECw (0.8 - control, 1.6, 2.4, and 3.2 dS m-1) and four concentrations of hydrogen peroxide - H2O2 (0, 10, 20, and 30 µM), with three replicates. Foliar application of hydrogen peroxide at a concentration of 10 µM increased growth, chlorophyll synthesis, and relative water content in the leaves and consequently reduced the foliar water saturation deficit of soursop irrigated with ECw up to 1.6 dS m-1. The concentration of hydrogen peroxide of 30 µM intensified the salt stress on the electrolyte leakage in the leaf blade and the photosynthetic pigments of soursop, 270 days after transplanting.


Assuntos
Annona , Peróxido de Hidrogênio , Peróxido de Hidrogênio/farmacologia , Fotossíntese , Folhas de Planta/fisiologia , Estresse Salino
11.
Braz J Biol ; 82: e262664, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36197402

RESUMO

The excess of salts present in the water can limit the hydroponic cultivation of melon in semi-arid regions of the Brazilian Northeast, making it necessary to use strategies that allow the use of these waters. Among these strategies, the use of elicitor substances stands out, such as salicylic acid. In this context, this study aimed to evaluate the effect of foliar application of salicylic acid in mitigating the harmful effects of salt stress on the morphophysiology and production of 'Gaúcho' melon cultivated in a hydroponic system. A completely randomized design was adopted in a split-plot scheme, with four levels of electrical conductivity of the nutrient solution - ECsn (2.1, 3.2, 4.3, and 5.4 dS m-1) considered the plots and four salicylic acid concentrations - SA (0, 1.5, 3.0, and 4.5 mM), the subplots, with six replications. The foliar application of salicylic acid concentrations did not mitigate the deleterious effects of salt stress on the morphophysiology and yield of melon grown in hydroponic system. The concentration of 4.5 mM of salicylic acid intensified the harmful effects of the salinity of the nutrient solution on gas exchange and fresh weight of hydroponic melon.


Assuntos
Cucurbitaceae , Ácido Salicílico , Hidroponia , Ácido Salicílico/farmacologia , Estresse Salino , Sais , Água
12.
J Pediatr Urol ; 17(3): 295-301, 2021 06.
Artigo em Inglês | MEDLINE | ID: mdl-33712372

RESUMO

OBJECTIVE: To analyze the effect of electrical nerve stimulation on urinary symptoms in pediatric patients with monosymptomatic primary enuresis refractory to conventional treatment. METHODS: Three databases (Medline, Embase, and Cochrane) were searched and 160 studies were identified by July 15, 2020. After establishing and applying the inclusion and exclusion criteria, a step-by-step analysis was performed using the title, abstract and full text. The Cochrane Collaboration Tool was then used to analyze the biases of the selected studies. RESULTS: Of the 160 articles found, 03 were selected for this systematic review. In 02 studies there was a significant reduction in the number of wet nights/week after electrical nerve stimulation. Urodynamic pattern was evaluated in 01 study, with improvement of maximum cystometric capacity in the intervention group. About maximum voided volume, there was no improvement in 01 study, but in other, there was increase in the intervention group. CONCLUSION: Electrical nerve stimulation might promote improvement in partial and total response scores over the number of dry nights, with no improvement in urodynamic parameters, and could be considered as an feasible option in the management of refractory monosymptomatic primary enuresis. However, it is worth emphasizing the need to conduct more RCTs with a larger sample for better evaluation of the role of neurostimulation.


Assuntos
Terapia por Estimulação Elétrica , Enurese , Enurese Noturna , Criança , Humanos , Enurese Noturna/terapia , Micção , Urodinâmica
13.
Int Endod J ; 43(9): 792-7, 2010 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-20579134

RESUMO

AIM: To evaluate the radiopacity, degree of conversion (DC) and flexural strength of an experimental dental cement, with several added radiopaque substances. METHODOLOGY: Titanium dioxide, quartz, zirconia, bismuth oxide, barium sulphate and ytterbium trifluoride were added to the experimental cement in five different concentrations. Radiopacity was evaluated with a phosphor plate system, and the radiodensity of specimens was compared with an aluminium step-wedge. DC was evaluated with FT-infrared spectroscopy following 20 s of photo-activation. Specimens with dimensions of 12 x 2 x 2 mm were used for the flexural strength test. Data were analysed with two-way anova and Tukey's post hoc test. RESULTS: Radiopacity of the experimental dental cements with barium sulphate and bismuth oxide at 40% and ytterbium fluoride at 30% and 40% showed no significant differences in comparison with 3 mm of Al (181, 96). CONCLUSIONS: The experimental dental cements with at least 30% added ytterbium trifluoride had satisfactory radiopacity without influencing other properties.


Assuntos
Meios de Contraste/química , Cimentos Dentários/química , Fluoretos/química , Itérbio/química , Ácido 4-Aminobenzoico/química , Absorciometria de Fóton , Alumínio/química , Sulfato de Bário/química , Materiais Biocompatíveis/química , Bismuto/química , Bis-Fenol A-Glicidil Metacrilato/química , Cânfora/análogos & derivados , Cânfora/química , Humanos , Teste de Materiais , Metacrilatos/química , Transição de Fase , Processos Fotoquímicos , Maleabilidade , Polietilenoglicóis/química , Ácidos Polimetacrílicos/química , Quartzo/química , Espectroscopia de Infravermelho com Transformada de Fourier , Estresse Mecânico , Fatores de Tempo , Titânio/química , Ítrio/química , Zircônio/química , para-Aminobenzoatos
14.
Oper Dent ; 45(5): 467-472, 2020 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-32243254

RESUMO

This article describes an indirect composite restoration technique for diastema closure and tooth lengthening with a noninvasive approach using regularly available materials such as silicone, composite resin, and an adhesive system. The procedure resulted in occlusal and functional improvement, with diastema closure, protrusive guide adjustment, and an increase in central incisor length. The procedure provided an adequate proportion of the central incisors with an esthetically natural appearance. It also resulted in simple, fast, and accurate manufacturing with a noninvasive esthetic indirect rehabilitation compared with more invasive preparation of ceramic veneers.


Assuntos
Facetas Dentárias , Diastema , Resinas Compostas , Diastema/terapia , Estética Dentária , Humanos , Incisivo
15.
Oper Dent ; 44(5): 536-544, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30978159

RESUMO

This study investigated the stability of the optical properties of high-translucent shades of dental resin-based composites. Four commercial materials (Filtek Z350 XT, Opallis, Amelogen Plus, and IPS Empress Direct) and 14 non-Vita shades were tested. Disc-shaped specimens for each resin-based composite-shade combination (n=6) were evaluated at T0 (baseline), T1 (after 30 days of storage in water), and T2 (after 30 days of storage each in water and a coffee solution). Color measurements were performed according to the L'C'h' color system. Translucency Parameter (TP) and CIEDE2000 color difference (ΔE00) were calculated. Data were statistically analyzed at α = 0.05. Baseline TP values varied from 43 ± 1 to 55 ± 1. Changes in TP at T1 varied from -3.0% (Opallis T-Neutral) to 4.2% (Amelogen Plus Trans Orange), with no major differences from T0. At T2, most resin-based composites showed significantly increased opacity, with changes varying between -15.0% (Empress Direct Trans 20) and -2.7% (Z350 XT Blue). However, the TP values were ≥40 throughout the study. Storage in water caused negligible color differences, with ΔE00 values at T1 ≤ 0.9 ± 0.6. At T2, all materials tested showed significant color difference, and ΔE00 ≥ 3.2 ± 0.2. The orange shades from Opallis and Amelogen Plus showed lower color variation than did the other shades. The most significant optical changes upon storage were detected in the hue and particularly the chroma color coordinate. In conclusion, the high-translucent resin-based composites showed large variability in the stability of their optical properties among the tested brands and different shades of the same material. Regardless of the storage condition, the tested resin-based composites retained their high-translucency character over time.


Assuntos
Resinas Compostas , Materiais Dentários , Cor , Colorimetria , Teste de Materiais
16.
J Virol Methods ; 263: 20-23, 2019 01.
Artigo em Inglês | MEDLINE | ID: mdl-30366017

RESUMO

Viruses belonging to the genus Begomovirus (family Geminiviridae) have circular single-strand DNA genomes encapsidated into quasi-icosahedral particles, and are transmitted by whiteflies of the Bemisia tabaci complex. Biological and molecular properties of begomoviruses have been studied efficiently with infectious clones containing dimeric genomic components. However, current approaches employing enzymatic digestion and ligation to binary vectors are laborious, mostly due to many cloning steps or partial digestion by restriction enzyme. Here, an infectious clone of the bipartite begomovirus Bean golden mosaic virus (BGMV) was obtained using PCR and Gibson Assembly (GA). Common bean (Phaseolus vulgaris) seedlings displayed severe yellow mosaic and stunt symptoms 15 days after agroinoculation with DNA-A and DNA-B of BGMV. The approach based on PCR-GA protocol is a fast and useful tool to obtain infectious clones of a circular DNA plant virus.


Assuntos
Begomovirus/genética , Clonagem Molecular/métodos , DNA Circular/genética , Genoma Viral/genética , Reação em Cadeia da Polimerase/métodos , Agrobacterium tumefaciens/genética , Begomovirus/patogenicidade , DNA Viral/genética , Phaseolus/virologia , Doenças das Plantas/virologia , Plântula/virologia
17.
Oper Dent ; 41(1): E13-22, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26509231

RESUMO

OBJECTIVE: The esthetics of the smile are related to the color, shape, texture, dental alignment, gingival contour, and the relationship of these with the face. PURPOSE: To present a two-year follow-up for an esthetic rehabilitation clinical case in which the method of digital smile design (DSD) was used to assist and improve diagnosis, communication, and predictability of treatment through an esthetic analysis of the assembly: face, smile, periodontal tissue, and teeth. CLINICAL PROCEDURE: The smile's esthetics were improved through gingival recontouring, dental home bleaching, and a restorative procedure with thin porcelain laminate veneers using lithium disilicate glass-ceramic (e.max Ceram, Ivoclar-Vivadent) laminates on teeth 4 through 13. DISCUSSION: The proposed technique had an acceptable clinical performance at the end of a two-year follow-up. SIGNIFICANCE: DSD can be used to increase professional/patient communication and to provide greater predictability for the smile's esthetic rehabilitation.


Assuntos
Facetas Dentárias , Estética Dentária , Porcelana Dentária , Seguimentos , Gengiva , Humanos , Sorriso
18.
Braz J Med Biol Res ; 27(6): 1291-7, 1994 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-7894342

RESUMO

1. Outer-membrane protein patterns of Escherichia coli recovered from the peritoneal cavities of infected guinea pigs and grown in medium M9 containing 2,2'-dipyridyl were studied by polyacrylamide gel electrophoresis (SDS-PAGE) to determine whether in vivo conditions of growth affected the expression of these bacterial surface proteins. 2. Eleven strains of septicemic E. coli studied in vitro under conditions of iron restriction expressed iron-regulated outer-membrane proteins, mainly the protein of approximately 74 kDa, whereas avirulent strains grown under similar conditions did not present the 74-kDa protein. 3. These results show the distribution of iron-regulated outer-membrane proteins among avian E. coli and suggest that the protein of approximately 74 kDa may be important for the virulence of these strains.


Assuntos
Proteínas da Membrana Bacteriana Externa/fisiologia , Escherichia coli/fisiologia , Ferro/fisiologia , 2,2'-Dipiridil , Animais , Galinhas , Eletroforese em Gel de Poliacrilamida , Escherichia coli/crescimento & desenvolvimento , Escherichia coli/patogenicidade , Cobaias
19.
Rev Bras Enferm ; 54(3): 511-6, 2001.
Artigo em Português | MEDLINE | ID: mdl-12227342

RESUMO

The present work presents a brief retrospective of the main facts that were part of the creation of the Brazilian Association of Nursing in Goiás Federal State. It identifies meaningful accomplishments of its administrations from 1948 up to 2001, pointing out its concerns, struggles and achievements based on documents such as book-proceedings, written documents, publications and reports of people who were involved with the association. It was concluded that this is an appropriate time for proposals which aim at the historical, political and social rescue of this association.


Assuntos
Sociedades de Enfermagem/história , Brasil , História do Século XX , Sociedades de Enfermagem/organização & administração
20.
Braz. j. med. biol. res ; 27(6): 1291-1297, June 1994.
Artigo em Inglês | LILACS | ID: lil-319775

RESUMO

1. Outer-membrane protein patterns of Escherichia coli recovered from the peritoneal cavities of infected guinea pigs and grown in medium M9 containing 2,2'-dipyridyl were studied by polyacrylamide gel electrophoresis (SDS-PAGE) to determine whether in vivo conditions of growth affected the expression of these bacterial surface proteins. 2. Eleven strains of septicemic E. coli studied in vitro under conditions of iron restriction expressed iron-regulated outer-membrane proteins, mainly the protein of approximately 74 kDa, whereas avirulent strains grown under similar conditions did not present the 74-kDa protein. 3. These results show the distribution of iron-regulated outer-membrane proteins among avian E. coli and suggest that the protein of approximately 74 kDa may be important for the virulence of these strains.


Assuntos
Animais , Cobaias , Escherichia coli , Ferro , Proteínas da Membrana Bacteriana Externa/fisiologia , 2,2'-Dipiridil , Galinhas , Eletroforese em Gel de Poliacrilamida , Escherichia coli
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