An efficient one-pot synthesis of heterocycle-fused 1,2,3-triazole derivatives as anti-cancer agents.

A series of heterocycle-fused 1,2,3-triazoles were easily prepared by the 1,3-dipolar cycloaddition of heterocyclic ketene aminals or N,O-acetals with sodium azide and polyhalo isophthalonitriles in a one-pot reaction at room temperature without a catalyst and evaluated in vitro against a panel of human tumour cell lines. 1,3-Oxazoheterocycle fused 1,2,3-triazoles were more potent against the tumour cell lines Skov-3, HL-60, A431, A549 and HepG-2 than 1,3-diazoheterocycle fused 1,2,3-triazoles. 4-Methoxyphenyl substituted 1,3-oxazoheterocycle fused 1,2,3-triazole 6j was found to be the most potent derivative with IC(50) values lower than 1.9 microg/mL against A431 and K562 human tumour cell lines.

[Molecular detection of specific HPV types in Condylomata acuminata].

To detect HPV in genital warts (Condylomata acuminata, CA) for infection rate and association of specific HPV types between males and females, and to provide support for the development of HPV vaccines, we designed HPV type-specific oligonucleotide primers to amplify DNA fragments encoding L1 viral capsule protein. SSP-PCR was conducted in duplication for each CA sample from male and female patients. DNA of TA-cloned HPV was used as positive control, and deionized H2O was used as negative control. A total of 22 clinical samples, 13 from males and 9 from females, was collected from patients diagnosed with CA at hospitals in Beijing and Handan. HPV viral DNA was amplified in all 22 samples analyzed, with 100% detection rate. TA-cloning and sequencing of the PCR products confirmed correct amplification of HPV type-specific fragments. Of the 13 samples from males, 5 were infected with HPV6, 6 with HPV11, and 2 with HPV6 + HPV11. Of the 9 samples from females, 3 were infected with HPV6, 2 with HPV11, and 4 with both HPV6 and HPV11 infection. In addition, high-risk types HPV16, HPV18, HPV33, HPV35, HPV45, HPV54, HPV56 and HPV58 were also detected in 4 female samples that were mixed with cervical cell debris during sample collection. However, no HPV types other than HPV6 and HPV11was detected in all CA-only samples in this study. We have established a sensitive and reliable laboratory procedure for HPV detection and classification. Using the method, we reached 100% detection rate of HPV in the CA samples. Our results confirm that HPV6 and HPV11 are primarily responsible for CA, and there is no specific association of HPV types between warts in males and females.

Sodium cobalt aminomethylidenediphosphonate with a novel open framework structure.

This paper reports the synthesis and crystal structure of a cobalt aminomethylenediphosphonate compound NaCo(2)[NH(3)CH(PO(3))(PO(3)H(0.5))](2)(H(2)O)(2).xH(2)O (1). It shows a novel open framework structure in which layers of Co(2)[NH(3)CH(PO(3))(PO(3)H(0.5))](2)(H(2)O)(2) are connected by NaO(6) linkages. The magnetic studies show a dominant antiferromagnetic exchange between the Co(II) ions.

An azide-bridged homospin single-chain magnet: [Co(2,2'-bithiazoline)(N3)2]n.

A novel end-on azide-bridged homospin 1D chain, Co(bt)(N3)2 (1) (bt = 2,2'-bithiazoline), is constructed by sharing edges of Co(II) distorted octahedrons to form a helix, which shows magnetic hysteresis with steps and slow relaxation below 5-6 K, typical of single-chain magnet behavior.

4-[N,N-Bis(2-cyanoethyl)amino]pyridine.

The title compound, 3,3'-(4-pyridylimino)dipropanenitrile, C(11)H(12)N(4), has a twofold axis and consists of a pyridine ring head and two cyanoethyl tails, the three groups being linked by an N atom. The planar geometry around the amino N atom suggests conjugation with the pi-system of the pyridine ring. The molecules are stacked in a layer structure via relatively weak to very weak intermolecular C-H.pi and C-H.N hydrogen-bond interactions.