Examining the Peer-Reviewed Literature on Tobacco-Related Social Media Data: Scoping Review.

INTRODUCTION: Tobacco researchers have used social media data to examine tobacco industry marketing practices (eg, influencers), and to document user experience with tobacco products. This study summarized the literature that analyzed tobacco-related social media data, including domain, social media platform, tobacco product type, and themes of findings, among other variables. AIMS AND METHODS: PubMed, PsycINFO, Web of Science, and Communication Source were searched between 2004 and 2022. Peer-reviewed articles were included if they were written in English, included at least one tobacco-related term, and one social media-related term, and analyzed a social media post. Two coders screened all-titles and abstracts. The final sample consisted of (n = 255) articles. Studies were coded for domain, social media platform, tobacco product type, data source, type of data, coding and analytic method, and presence of validation procedure, among other variables. RESULTS: A total of 10 504 820 581 tobacco-related social media posts were assessed across 255 studies. User experience (54.1%) and promotion (23.1%) were the most researched domains. Researchers used data from Twitter the most (42.7%). Text (43.1%) was the most common type of data analyzed. Thematic analysis (80.8%) was the most common analytic technique. Themes of findings from content analyses often pertained to the health effects of tobacco use (61.0%) and promotion (44.2%). CONCLUSIONS: Researchers have analyzed billions of tobacco-related social media posts to describe user experience with, and promotions related to, tobacco products like e-cigarettes on platforms like Twitter. Future research may examine tobacco-related social media data from newer platforms like TikTok. IMPLICATIONS: Real-time surveillance of tobacco-related content on social media can keep the tobacco control community abreast of tobacco industry promotional strategies, user experience with tobacco products, and perceived health effects of tobacco use. A framework may be developed to establish best-practices for social media data collection and analysis, including strategies to identify posts from bot accounts and validate methodological approaches used in thematic analysis.

Analytical performance of the FDA-cleared Parsortix® PC1 system.

Introduction: The Parsortix® PC1 system, Food and Drug Administration (FDA) cleared for use in metastatic breast cancer (MBC) patients, is an epitope-independent microfluidic device for the capture and harvest of circulating tumor cells from whole blood based on cell size and deformability. This report details the analytical characterization of linearity, detection limit, precision, and reproducibility for this device. Methods: System performance was determined using K2-EDTA blood samples collected from self-declared healthy female volunteers (HVs) and MBC patients spiked with prelabeled cultured breast cancer cell lines (SKBR3, MCF7, or Hs578T). Samples were processed on Parsortix® PC1 systems and captured cells were harvested and enumerated. Results: The system captured and harvested live SKBR3, MCF7, and Hs578T cells and fixed SKBR3 cells linearly between 2 and ~100 cells, with average harvest rates of 69%, 73%, 79%, and 90%, respectively. To harvest ≥1 cell ≥95% of the time, the system required 3, 5 or 4 live SKBR3, MCF7 or Hs578T cells, respectively. Average harvest rates from precision studies using 5, 10, and ~50 live cells spiked into blood for each cell line ranged from 63.5% to 76.2%, with repeatability and reproducibility percent coefficient of variation (%CV) estimates ranging from 12.3% to 32.4% and 13.3% to 34.1%, respectively. Average harvest rates using ~20 fixed SKBR3 cells spiked into HV and MBC patient blood samples were 75.0% ± 16.1% (%CV = 22.3%) and 68.4% ± 14.3% (%CV = 21.1%), respectively. Conclusions: These evaluations demonstrate the Parsortix® PC1 system linearly and reproducibly harvests tumor cells from blood over a range of 1 to ~100 cells.

Type II endometrial cancers with minimal, non-invasive residual disease on final pathology: What should we do next?

There are minimal data regarding the management of high risk endometrial cancer histologies lacking invasive disease on the final pathology specimen. This study examines a cohort of these patients and assesses outcomes including time to recurrence and risk of death after management with and without adjuvant therapies. Endometrial cancer patients with minimal or no remaining invasive disease on final pathologic specimen from 1995 to 2010 were included. Surgical procedure was at the discretion of the operating physician. Electronic medical records were used to abstract relevant clinicopathologic data and standard statistical methods were employed. 70 patients met inclusion criteria, of which 26 were high grade histologies. Adjuvant therapies were given in 12 of 26 patients. 6/26 patients recurred, of which 50% were salvaged with therapy at time of recurrence. Overall deaths occurred in 3 of 26 patients in the high risk cohort. Less than half of the high risk cohort received adjuvant therapies after surgical management. No histologic type was found to increase risk of recurrence, and treatment with initial adjuvant therapy did not significantly reduce recurrence risk. Large scale prospective trials are needed to aid in management of this unique endometrial cancer population.

Development of a clinical prediction rule for diagnosing hip osteoarthritis in individuals with unilateral hip pain.

STUDY DESIGN: Prospective cohort/predictive validity study. OBJECTIVE: To determine the diagnostic accuracy of common clinical examination items and to construct a preliminary clinical prediction rule for diagnosing hip osteoarthritis (OA) in individuals with unilateral hip pain. BACKGROUND: The current gold standard for the diagnosis of hip OA is a standing anteroposterior (AP) radiograph of the pelvis. Other than for Altman's criteria, little research has been done to determine the accuracy of clinical examination findings for diagnosing hip OA. METHODS AND MEASURES: Seventy-two subjects completed the study. Each subject received a standardized history, physical examination, and standing AP radiograph of the pelvis. Subjects with a Kellgren and Lawrence score of 2 or higher based on the radiographs were considered to have definitive hip OA. Likelihood ratios (LRs) were computed to determine which clinical examination findings were most diagnostic of hip OA. Potential predictor variables were entered into a logistic regression model to determine the most accurate set of clinical examination items for diagnosing hip OA. RESULTS: Twenty-one (29%) of the 72 subjects had radiographic evidence of hip OA. A clinical prediction rule consisting of 5 examination variables was identified. If at least 4 of 5 variables were present, the positive LR was equal to 24.3 (95% confidence interval: 4.4-142.1), increasing the probability of hip OA to 91%. CONCLUSION: The preliminary clinical prediction rule provides the ability to a priori identify patients with hip pain who are likely to have hip OA. A validation study should be done before the rule can be implemented in routine clinical practice.

Genital injuries acute evaluation and management.

Genital trauma may result in external injuries to the labia, vulva or vagina, urethra and anus and internal injuries to the bony pelvis, bladder, bowels and reproductive organs. Worldwide, the most common cause of genital trauma in reproductive age women is injury sustained during childbirth, but in this chapter we will focus on accidental genital injuries as well as those arising from sexual violence, and female genital mutilation. While genital injuries alone rarely result in death; if not properly managed, chronic discomfort, dyspareunia, infertility, or fistula formation may result. Clinicians need to be able to recognize these injuries and provide initial management, and assure that the patient's mental, emotional and physical needs are addressed.

Preliminary validation of the military low back pain questionnaire.

Soldiers must perform a variety of physical tasks that the civilian population does not. The Modified Oswestry Disability Index (M-ODI) is the most widely used measure of function in patients with low back pain but does not include military tasks. The Military Low Back Pain Questionnaire (MBQ) was developed by military Physical Therapists to include tasks such as wearing body armor. The purpose of this study was to provide preliminary evidence for the reliability, responsiveness, and validity of the MBQ in nondeployed Soldiers. The MBQ had good reliability compared to the M-ODI. The inter-rater correlation coefficient for the M-ODI was 0.79 and 0.75 for the MBQ. Cronbach's alpha was 0.75 and 0.85 for the M-ODI and MBQ, respectively. The minimal detectable change for the M-ODI was 21.03 and 22.97 for the MBQ. Responsiveness was assessed using a global rating of change; area under the curve for the M-ODI was 0.82 and 0.90 for the MBQ. The correlation between the M-ODI and the MBQ was r = 0.80 indicating good concurrent validity. The MBQ was as reliable as the M-ODI in an Army population. There were trends in the psychometrics suggesting the MBQ may be more sensitive to change than the M-ODI in this population.

A comparison of deployed occupational tasks performed by different types of military battalions and resulting low back pain.

With deployment Soldiers must now wear body armor and additional equipment while performing occupational tasks, representing a large demand that has not been considered when studying military occupations. The purpose of this study was to: (1) describe tasks required by different occupational battalions within a Brigade Combat Team; (2) establish the incidence of low back pain (LBP) in each battalion and; (3) determine which tasks predict LBP within the different battalions. This was a prospective cohort study investigating 805 Soldiers in a Brigade Combat Team deployed to Afghanistan for 1 year. Demographic, occupational, and fitness variables were recorded. There was no difference in time spent on fitness training between the battalions. Occupational tasks performed by deployed Soldiers vary in the level of physical demand between battalions. Infantry had the highest fitness score (257); wore the heaviest equipment (70 lb.); spent the most time wearing body armor (49 hours/week), performing dismounted patrol (29 hours/week), and lifting objects (35 hours/week); spent the least amount of time working at a desk (14 hours/week); but had a similar incidence of LBP (77%) compared to other battalions. History of LBP and time spent wearing body armor were the two most consistent predictors of LBP across battalion types.

Loads worn by soldiers predict episodes of low back pain during deployment to Afghanistan.

STUDY DESIGN: Prospective cohort study. OBJECTIVE: To describe low back pain (LBP) sustained by soldiers deployed to Afghanistan and identify demographic, fitness, and occupational risk factors of LBP. SUMMARY OF BACKGROUND DATA: LBP is the most common injury in deployed soldiers. It results in lost duty days, medical evacuations, and permanent disability. Risk factors for LBP have not been investigated in this population. METHODS: This study investigated a US Army Brigade Combat Team deployed to Afghanistan for 1 year. Demographic, fitness, and occupational variables were recorded before and after deployment. Episodes of LBP were assessed using self-report data. Logistic regression was used to investigate predictors of (1) moderate or worse LBP developed during deployment and (2) all LBP developed during deployment. RESULTS: The incidence of moderate or worse LBP was 22% in the 805 soldiers studied. Soldiers with elevated risk of LBP were male, in Cavalry or Infantry units, wore body armor for more than 6 hr/d, worked at a desk for 1 to 4 hours a day, spent 5 to 8 hr/d lifting, spent more than 1 hr/wk on walking patrol, spent 21 to 40 hr/wk in a tactical vehicle, or wore equipment. The most parsimonious set of significant predictors for moderate or worse LBP included age, OR = 1.04 (1.01-1.08); fitness score, OR = 0.99 (0.989-0.999); the amount of time spent wearing body armor, OR = 1.16 (1.10-1.23); the amount of time spent on walking patrol, OR = 1.01 (1.003-1.02); and weight of the equipment worn, OR = 1.01 (1.002-1.013). The incidence of all LBP (minor or worse) was 77%. The most parsimonious set of significant predictors for all LBP included age, OR = 1.06 (1.02-1.09); fitness score, OR = 0.99 (0.985-0.997); history of LBP, OR = 2.94, (1.87-4.62); equipment weight, OR = 1.008 (1.003-1.01); and time spent wearing body armor, OR = 1.13 (1.07-1.19). CONCLUSION: This study found that being older, having lower fitness scores, wearing armor longer, and wearing heavier loads increased the risk of LBP in deployed soldiers. Units more likely to wear loads such as the Infantry and Cavalry and tasks commonly involving wearing loads such as patrolling all increased the risk of LBP as well.

Dietary supplementation of Ashwagandha (Withania somnifera, Dunal) enhances NK cell function in ovarian tumors in the laying hen model of spontaneous ovarian cancer.

PROBLEM: Ovarian cancer (OVCA) disseminates in a distinct pattern through peritoneal metastasis and little is known about the immunosuppression in the tumor microenvironment. Our goal was to determine changes in NK cell population during OVCA development and the effects of Ashwagandha (Withania somnifera, Dunal) supplementation on NK cell localization in laying hens with OVCA. METHODS: Frequency of NK cells in ovarian tumors at early and late stages in 3- to 4-year-old hens (exploratory study) as well as in hens supplemented with dietary Ashwagandha root powder for 90 days (prospective study) was examined. RESULTS: The population of stromal NK cells but not the intratumoral NK cells increased with OVCA development and progression. Ashwagandha supplementation decreased the incidence and progression of OVCA. Both the stromal and intratumoral NK cell population increased significantly (P < 0.0001) in Ashwagandha supplementated hens. CONCLUSION: The results of this study suggest that the population of stromal and tumorinfiltrating NK cells is increased by dietary Ashwagandha supplementation. Thus, Ashwagandha may enhance antitumor function of NK cells. This study may be useful for a clinical study to determine the effects of dietary Ashwagandha on NK cell immune function in patients with ovarian cancer.