Morphine induces HADHA succinylation, while HADHA desuccinylation alleviates morphine tolerance by influencing autophagy.

Morphine tolerance is an important factor in unsatisfactory analgesia. HADHA is a crucial enzyme in fatty acid ß-oxidation. In this study, we investigated the potential significance of HADHA in a mechanism that might cause morphine tolerance related to functional changes in energy metabolism and further explored the effect of HADHA desuccinylation on morphine tolerance. Rats received daily intrathecal injections of 10 µg of morphine for a duration of 7 consecutive days, and pain thresholds were measured using the mechanical withdrawal threshold (MWT) and thermal tail flick latency (TFL) tests. µ-Opioid receptor (MOR), LC3-I/II, and P62 expression and HADHA succinylation were assessed. HADHA succinylation was analyzed by liquid chromatography-tandem mass spectrometry (LC‒MS/MS) and parallel reaction monitoring (PRM). Morphine influenced the LC3II/LC3I ratio and P62 expression level, which are crucial indicators of autophagy, and stimulated HADHA succinylation. Additionally, HADHA was selectively bound by the desuccinylase SIRT5, and SIRT5 overexpression decreased HADHA succinylation, reduced P62 expression, and alleviated morphine tolerance.

Psychometric Evaluation of the Disaster Preparedness Evaluation Tool© (DPET) on Emergency Nurses in Mainland China: Two Cross-Sectional Studies.

BACKGROUND: Emergency nurses play a major role in disaster relief in mainland China, but there is no valid instrument to measure the extent of their disaster preparedness. The Disaster Preparedness Evaluation Tool© is a reliable instrument to assess the disaster preparedness of nurse practitioners. The tool has been translated and validated in Saudi Arabia, Taiwan, China and the United States of America. OBJECTIVES: This study aimed at translating and adapting the Disaster Preparedness Evaluation Tool© (DPET) for emergency nurses in mainland China and determining its psychometric properties. DESIGN, SETTINGS AND PARTICIPANTS: A total of 2 cross-sectional online surveys were conducted in the emergency departments of 26 public grade III-A hospitals in Guangdong, mainland China. In the first study, 633 emergency nurses were recruited from May to August, 2018. In the second study, 205 were recruited in April 2019. METHODS: The instrument was adapted through rigorous forward-backward translation, face validity, and pre-test processes. Exploratory factor and parallel analyses were used in the first study. Confirmatory factor analysis, internal consistency and split-half reliability were used in the second study. RESULTS: Exploratory factor and parallel analyses extracted a 5-factor solution comprising of 34 items that accounted for 64.06% of the total variance. The fit indices indicated a good model fit. The reliability was good, as indicated by a Cronbach's alpha of 0.97 and a split-half reliability coefficient of 0.97. CONCLUSION: The mainland China version of the DPET (DPET-MC) was a reliable and valid instrument and can be used in practice.

Disaster Preparedness and Associated Factors Among Emergency Nurses in Guangdong Province, China: A Descriptive Cross-Sectional Study.

OBJECTIVE: The purpose of this study was to examine the prevalence of disaster preparedness and to explore associated factors among emergency nurses in Guangdong Province, China. METHODS: In this descriptive, cross-sectional study, the mainland China version of the Disaster Preparedness Evaluation Tool was used to collect data from 633 nurses working in 26 emergency departments, in August 2018. Descriptive analyses were used to examine the disaster preparedness, and multiple linear regression analysis was used to investigate associated factors. RESULTS: The perceived disaster preparedness of emergency nurses was at a moderate level. Among the 5 dimensions, the score for disaster management was lowest. Emergency nurses' disaster training (r = .26; P < 0.001) and drill experiences (r = .22; P < 0.001) were significantly correlated with disaster preparedness. Six significant factors associated with disaster preparedness were identified in this study: age, gender, disaster training experience, disaster drill experience, willingness, and educational level (R2 = .14; F = 18.20; P < 0.001). CONCLUSIONS: Hospitals and nurse managers should carry out interdisciplinary and multidisciplinary cooperation to improve emergency nurses' disaster preparedness, especially disaster management. Organizing disaster simulation exercises, providing psychological support and safety considerations, and formulating disaster nursing training programs may be beneficial for emergency nurses' disaster preparedness.

Coupling and coordinated development of new urbanization and agro-ecological environment in China.

Agricultural ecological environment conservation is a major strategic mission for the sustainable development of China's agriculture and rural areas at present. Therefore, it is of great significance to examine the coupling and coordination relationship between new urbanization and the agro-ecological environment, and, accordingly, to establish China's sustainable agro-economic development model. However, this topic has not been adequately addressed in previous studies. To fill this gap, this study detailed an empirical investigation into the spatio-temporal differentiation of and influencing factors on the coupling and coordinated development of new urbanization and the agricultural ecological environment in China. First, aided by spatial analysis methods, a detailed analysis of the spatio-temporal patterns and dynamics of the coupling and coordinated development of new urbanization and the agro-ecological environment was conducted. The driving factors were quantitatively examined using the random panel Tobit model and the panel data modeling technique. The results indicate that the coupling degree between China's new urbanization and the agro-ecological environment ranges from 0.30 to 0.50, which is in the antagonistic stage. The degree of coupling and coordination is between 0.39 and 0.59, basically on the verge of imbalance and reluctant coordination. Most provinces lag behind in new urbanization. Panel data analysis demonstrates financial support for agriculture, rural economic development, environmental pollution control capabilities, and government capabilities significantly impacting the degree of coupling and coordination between China's new urbanization and the agro-ecological environment. Among them, financial support for agriculture and industrialization has different effects on the degree of coupling and coordination between the eastern and midwestern regions. Based on the findings of this study, a series of policy suggestions for improving China's new urbanization and agro-ecological environment is proposed.

Insight the roles of loosely-bound and tightly-bound extracellular polymeric substances on Cu2+, Zn2+ and Pb2+ biosorption process with Desulfovibrio vulgaris.

The aim of this study is to explore the fate and mechanism of metal cations of biosorption in the Desulfovibrio vulgaris system (including bacterial cells and secreted loosely-bound extracellular polymeric substances (LB-EPS) and tightly-bound extracellular polymeric substances (TB-EPS)). The relative contribution of EPS (TB-EPS and LB-EPS) to the adsorption of three metal cations is much greater than that of bacterial cells, and the adsorption capacity of Pb2+ on EPS (TB-EPS and LB-EPS) is much greater than that of Cu2+ and Zn2+ (Pb2+ > Cu2+ > Zn2+). The order of absorption capacity was as follows: LB-EPS > TB-EPS > bacterial cells, the adsorption contribution of EPS (including TB-EPS and LB-EPS) to Cu2+, Zn2+ and Pb2+ accounted for total adsorption capacity was 82%, 83% and 86%, respectively. It was suggested that LB-EPS was the first reaction barrier of immobilization metal cations before metal cations was able to pass through EPS and react with cells. The adsorption process was dominated by complexation and electrostatic interaction. The three-dimensional excitation-emission matrix (3D-EEM) identified two main fluorescence peaks of the aromatic and tryptophan protein-like substances in EPS. According to the synchronous fluorescence spectra, the tryptophan protein-like substances were gradually quenched with increased metal cations concentrations, which the quencher mechanism is dynamic quenching. The findings of this work are significant to reveal the fate of Cu2+, Zn2+ and Pb2+ during its sorption process onto Desulfovibrio vulgaris, and provide useful information of the interaction between Desulfovibrio vulgaris and its secreted EPS with metal cations.