The potential of Ethiopian medicinal plants to treat emergent viral diseases.

Ethiopians have deep-rooted traditions of using plants to treat ailments affecting humans and domesticated animals. Approximately 80% of the population continues to rely on traditional medicine, including for the prevention and treatment of viral diseases. Many antiviral plants are available to and widely used by communities in areas where access to conventional healthcare systems is limited. In some cases, pharmacological studies also confirm the potent antiviral properties of Ethiopian plants. Building on traditional knowledge of medicinal plants and testing their antiviral properties may help to expand options to address the global pandemic of COVID-19 including its recently isolated virulent variants and prepare for similar outbreaks in the future. Here, we provide an ethnobotanical and pharmacological inventory of Ethiopian medicinal plants that might contribute to the prevention and treatment of viral diseases. We identified 387 species, about 6% of Ethiopia's known flora, for which records of use by local communities and traditional herbalists have been documented for the treatment of viral diseases. We provide a framework for further investigation and development of this vital resource much anticipated to help combat emergent viral diseases along with existing ones in Ethiopia and elsewhere.

Medicinal plants used to treat livestock ailments in Ensaro District, North Shewa Zone, Amhara Regional State, Ethiopia.

BACKGROUND: In Ethiopia, the majority of animal owners throughout the country depend on traditional healthcare practices to manage their animals' health problems. This ethnoveterinary study was carried out in Ensaro District, North Showa Zone, and Amhara Region, Ethiopia, to identify medicinal plant species used by the local community to treat various livestock ailments. METHODS: To collect ethnobotanical information, a total of 389 informants (283 men and 106 women) were selected. Among these 95 traditional medicine practitioners were purposely chosen, while the remaining 294 were selected through a systematic random sampling method. Ethnobotanical data were collected through semi-structured interviews, participant observation, guided filed walks and focus group discussions. The Informant Consensus Factor (ICF) and Fidelity Level (FL) values, preference, and direct matrix exercise ranking were determined using quantitative methods. Statistical tests were used to compare indigenous knowledge of medicinal plants among different informant groups. RESULTS: A total of 44 ethnoveterinary medicinal plant species were collected and identified that were distributed across 43 genera and 28 families. The family Solanaceae stood first by contributing 4 species followed by Amaranthaceae, Asteraceae, Euphorbiaceae, Fabaceae and lamiaceae with 3 species each. Thirty- seven (88.09%) medicinal plants were collected from wild habitats, 6 medicinal plant species were collected from home garden. The most frequently used life form was shrubs (23 species, 54.76%) followed by herbs (13 species, 30.95%). The widely used parts of medicinal plants were leaves followed by roots. Prepared remedies were administered through drenching, dropping, smearing, eating, wrapping, fumigating and washing. There was significant difference in the indigenous knowledge of medicinal plants between gender, urban and rural people, general and key informants and among age groups. CONCLUSION: Ensaro District is a relatively rich in diversity of ethnoveterinary medicinal plants together with a rich indigenous knowledge in the local communities to collect and effectively utilize for the management different livestock diseases. On the other hand, these days, agricultural expansion, fuel wood collection, cutting plants for fence, furniture and charcoal production are the major threatening factors of these plant resources. Thus, people of the study area must implement in situ and ex-situ conservation strategies to ensure sustainable utilization of these species.

Ethiopian Medicinal Plants Traditionally Used for the Treatment of Cancer, Part 2: A Review on Cytotoxic, Antiproliferative, and Antitumor Phytochemicals, and Future Perspective.

This review provides an overview on the active phytochemical constituents of medicinal plants that are traditionally used to manage cancer in Ethiopia. A total of 119 articles published between 1968 and 2020 have been reviewed, using scientific search engines such as ScienceDirect, PubMed, and Google Scholar. Twenty-seven medicinal plant species that belong to eighteen families are documented along with their botanical sources, potential active constituents, and in vitro and in vivo activities against various cancer cells. The review is compiled and discusses the potential anticancer, antiproliferative, and cytotoxic agents based on the types of secondary metabolites, such as terpenoids, phenolic compounds, alkaloids, steroids, and lignans. Among the anticancer secondary metabolites reported in this review, only few have been isolated from plants that are originated and collected in Ethiopia, and the majority of compounds are reported from plants belonging to different areas of the world. Thus, based on the available bioactivity reports, extensive and more elaborate ethnopharmacology-based bioassay-guided studies have to be conducted on selected traditionally claimed Ethiopian anticancer plants, which inherited from a unique and diverse landscape, with the aim of opening a way forward to conduct anticancer drug discovery program.

Ethnobotanical study of medicinal plants used to treat human ailments in Quara district, northwestern Ethiopia.

BACKGROUND: Ethiopia is recognized as one of the centers of origin for the diversity of many plant species, including medicinal plants. Throughout the country, a large proportion of the population relies on these therapeutic plant species for primary healthcare. While such traditional medicinal knowledge has been documented in some regions, there is a lack of information from the Quara district of northwestern Ethiopia. Therefore, this study aimed to document the indigenous and local knowledge of the use of medicinal plants among three ethnic groups residing in the area. METHODOLOGY: An ethnobotanical study was conducted from August 2022 to October 2023 in ten kebeles of the Quara district. Data were collected through semi-structured interviews, field walks, and focus group discussions with 286 informants using snowball, purposive, and random sampling techniques. Quantitative analyses included Rahman's similarity index (RSI), informant consensus factor (ICF), and direct matrix ranking (DMR). Descriptive statistics were used to analyze basic ethnobotanical data. RESULTS: A total of 128 medicinal plant species from 112 genera and 50 families were documented and used to treat 14 disease categories. There was a 28% overlap in medicinal plant knowledge among the three ethnic groups studied. Fabaceae was the most represented family with 22 species. Trees accounted for the majority of the documented plants (37.5%), and leaves were the most commonly used plant parts (23.1%). Oral administration (56.7%) of plant extracts was the primary mode of remedy preparation and use. The highest ICF value (0.93) was recorded for circulatory and blood-related disorders. The study identified nine plants and 39 therapeutic uses not previously reported in Ethiopia. The RSI showed high overlap with neighboring areas and low similarity with distant areas. Top-ranked multipurpose plants were Ziziphus spina-christi and Terminalia leiocarpa, with agricultural expansion and fuelwood collection identified as major threats. CONCLUSION: The findings demonstrate the rich diversity of medicinal plants and associated traditional knowledge in the Quara district. The high ethnobotanical indices warrant further phytochemical and pharmacological investigations. Integrated conservation efforts are recommended to address the challenges facing these valuable plant resources.

Ethnobotanical study of medicinal plants in Dibatie district, Metekel zone, Benishangul Gumuz Regional State, western Ethiopia.

BACKGROUND: Herbal medicine has been used for the treatment of human and livestock ailments since ancient times. Numerous rural and urban communities in Ethiopia practice traditional medicine and transfer the knowledge verbally from generation to generation. Thus, this study was conducted to document the traditional medicinal plants and associated indigenous knowledge in Dibatie district, Metekel zone, Benishangul Gumuz Regional State, western Ethiopia. METHODS: Three hundred seventy-four (374) informants from 11 kebeles (the smallest administrative units) were selected and participated in the data delivery. The ethnobotanical data collection was carried out using semi-structured interviews, preference ranking, direct matrix ranking, field observation, market surveys, and focus group discussions, including voucher specimen collections. The ethnobotanical data were analyzed using descriptive statistics (frequency and percentage), ranking, comparison, and quantitative ethnobotanical techniques such as informant consensus factor, fidelity level index, Jaccard's coefficient of similarity, and use value index. RESULTS: A total of 170 plant species were recorded to treat 79 human and 29 livestock ailments. Fabaceae (with 20 species) and Asteraceae (with 18 species) were the most dominant medicinal plant families in the area. Most remedial plants were herbs (61 species, 35.88%), followed by shrubs (39 species, 22.94%). The majority (135 species, 79.41%) of medicinal plants were harvested from wild sources and mainly possessed multiple remedy parts (41.17%) that are usually prescribed in fresh form (60.13%). The most commonly reported human ailment was snake venom, while blackleg was mostly reported among livestock diseases. The herbal medicines were mostly administered orally (52.20%), followed by dermal (17.62%) application. Embelia schimperi Vatke, Glinus lotoides L., Haplosciadium abyssinicum Hochst., Mucuna melanocarpa Hochst. ex A. Rich., and Phragmanthera macrosolen (Steud. ex A. Rich.) M.G.Gilbert had the highest fidelity level values (100%) against the corresponding ailments. CONCLUSION: The study area is rich in a diversity of potential medicinal plants and associated indigenous knowledge. Thus, appropriate conservation actions and careful utilization are essential to counteract the rise of anthropogenic threats and to ensure the continuity of plants with the related indigenous knowledge. Additionally, the medicinal plants should be validated through experimentation to integrate local knowledge with modern medications.

A systematic review exploring the diversity and food security potential of wild edible plants in Ethiopia.

Wild edible plants (WEPs) are important food sources globally due to their accessibility and affordability. In Ethiopia, where diverse cultural groups consume WEPs, this systematic review explores their diversity, edible parts, and role in supporting food security. The review examined 38 original studies on the ethnobotany of WEPs in Ethiopia from 2000 to 2022. It identified a total of 651 WEP species from 343 genera and 94 families, with the Fabaceae family having the most species (51). Herbs and shrubs were the predominant growth habits, and fruits were the most consumed plant parts. The review prioritized nine WEP species for cultivation and promotion. However, threats such as overgrazing, agricultural expansion, and the use of woody species for construction, firewood, and charcoal have depleted WEP resources and eroded traditional knowledge about their use. The review suggests that WEPs have the potential to contribute to food and nutritional security in Ethiopia if these threats are effectively managed. However, the limited coverage of ethnobotanical studies on WEPs requires further investigation. The study recommends integrating the prioritized WEPs into the national food system for promotion, cultivation, and nutrient analysis to evaluate their nutritional bioavailability.

Ethnobotanical study of wild edible plants in Dibatie district, Metekel zone, Benishangul Gumuz Regional State, western Ethiopia.

BACKGROUND: Plants deliver livelihood and food for millions of people in the world. Indeed, wild edible plants support rural communities in developing countries to overcome seasonal unfavorable conditions. In rural areas of Ethiopia, wild edible plants play an indispensable role in fighting food insecurity as emergency or supplementary foods. Hence, this research was aimed at studying the ethnobotanical assessment of wild edible plants in Dibatie district, Metekel zone, western Ethiopia. METHODS: Ethnobotanical data was collected using a semi-structured interview, field observation, focus group discussions, a market survey, and the ranking of selected plants. Besides, voucher specimens were collected and stored at the National Herbarium of Ethiopia. Descriptive statistics, preference ranking, direct matrix ranking, and familiarity index were computed for data analysis. RESULTS: This study has documented 54 wild edible plant species belonging to 33 plant families and 46 genera. Of these, most (38.90%) had tree growth habits. Wild edible plants bear mostly fruits (72.20%) as edible parts. Local people usually consume these plants freshly raw as complementary foods, though some wild edibles require processing. They were mostly harvested in the January (31.48%) and May (27.78%) months, with the least collected in September (7.41%). Most wild edible plants (78.57%) were available in uncontrolled habitats, while others (21.43%) live in farmlands, home gardens, and as live fences. Out of the recorded plants, about 98% had additional uses besides their nutritional values. CONCLUSION: Wild edible plants assist the livelihoods of the local people in food security, agriculture, energy sources, construction, medicines, ecological services, aesthetics, income generation, and household utensils. Nevertheless, wild edible plants are recently threatened due to various anthropogenic factors in the study area. Thus, they need wise use and in-situ and ex-situ conservation measures from all the concerned bodies for sustainable use in the future.

Ethnoveterinary medicinal plants and their utilization by indigenous and local communities of Dugda District, Central Rift Valley, Ethiopia.

BACKGROUND: Ethnoveterinary medicinal plants have been used by the people of Dugda District in the primary health care system to treat various livestock ailments for generations, particularly, in underserved rural areas. However, these ethnoveterinary practices and medicinal plants are dwindling without proper documentation, due to undergoing socio-cultural and environmental changes. Hence, this study aimed at inventory and analysis of ethnoveterinary medicinal plants and the associated indigenous and local knowledge used in the treatment of livestock health problems in Dugda District. METHODS: Data were collected from 378 local inhabitants through semi-structured interviews, 18 focus group discussions with 6 to 8 participants in each couple with participant field observations. Informant consensus factor (ICF), fidelity-level (FL) and relative importance value (RI) were used to evaluate the agreement of informants on ethnoveterinary practices, healing potential of medicinal plants and the most multipurpose species. Using standard taxonomic procedures, voucher specimens were collected, identified and deposited at the National Herbarium of Addis Ababa University. RESULTS: In total, 64 medicinal plants of ethnoveterinary uses, belonging to 33 families were reported to treat 37 livestock health problems. Anthrax, inappetence and diarrhoea were said to be the most prevalent veterinary health problems treated by traditional medicinal plants. Family Fabaceae was the most widely reported (7 spp.), followed by Apocynaceae, Cucurbitaceae and Solanaceae (5 spp. each). Herbs were the dominant life forms (21spp.), followed by shrubs (20 spp.). The most commonly sought plant parts were leaves (55.25%), followed by roots (23.44%). The principal method of preparation was pounding remedial parts (46.85%) and mixing with cold water. The main route of administration was via oral application (72.67%), drenching diseased livestock. Withania somnifera and Kedrostis foetidissima were the most cited medicinal plants with 53 and 43 use reports (URs), respectively. ICF showed that respiratory diseases scored the highest value (0.94), while most of the reported medicinal plants were gastrointestinal agents. RI value analysis revealed that Croton macrostachyus had highest diversity of uses, followed by K. foetidissima and W. somnifera. Ethnoveterinary uses of some medicinal plants such as Phytolacca dodecandra, Calpurnia aurea, Cyphostemma cyphopetalum and Verbascum sinaiticum as prophylaxis against rabies were a new input for ethnoveterinary database. CONCLUSION: The study revealed that the people of the Dugda District are endowed with rich ethnoveterinary knowledge and medicinal plants. However, ethnoveterinary knowledge associated with medicinal plant species significantly differ (P < 0.05) between general and key informants, young and matured age groups and informants' educational level. This infers the impact of mode of ethnoveterinary knowledge transfer, literacy, modern education and globalization on ethnoveterinary uses of medicinal plant species. And also most of the medicinal plants are found in wild habitats with nearly no conservation attention. Therefore, it is imperative to implement participatory conservation actions for medicinal plants in collaboration with traditional institutions (Gada system). The disparity of ethnoveterinary knowledge could be also minimized through awareness creation among local communities about the knowledge and its revitalization. Furthermore, medicinal plants, which are widely utilized and multipurpose, should be screened for their phytochemicals, pharmacological and toxicological activities to confirm ethnoveterinary uses and for future development of veterinary pharmaceuticals.

Ethnobotanical study of traditional medicinal plants used by the local people in Habru District, North Wollo Zone, Ethiopia.

BACKGROUND: Ethiopia is a country located in the Horn of Africa, which combines richness in plant resources and cultures of human plant use. The people of Habru District of North Wollo Zone (Amhara Region, Ethiopia) have a long history of use of plant resources for various purposes including in traditional herbal remedy preparation and use. However, the district has not been adequately studied for its ethnobotanical resources and the associated knowledge. This study focused on human medicinal plants and their traditional uses in Habru District. The objective of the study was to document and analyze the plant species used by the local communities to treat human ailments along with the associated traditional knowledge and practices. METHODOLOGY: The study was carried out in Habru District from June 2021 to December 2022. Ethnobotanical data were collected using semi-structured interviews, guided field walks, 13 focus group discussions (one at the district level and 12 at the kebele/subdistrict level) and market surveys. A total of 388 informants (250 males and 138 females) were selected from all 13 kebeles within Habru District using systematic random sampling, and 42 key informants were purposively selected. Descriptive statistics, preference ranking, direct matrix ranking, informant consensus factor and fidelity level were applied for data analysis. RESULTS: The results provide insights into the medicinal plant diversity within Habru District, where 134 plant species in 110 genera and 54 families were documented, including 2 endemics, highlighting the district's significance in biodiversity conservation and healthcare delivery. Disease prevalence analysis showed that gastrointestinal and parasitic ailments (ICF = 0.85), febrile diseases (ICF = 0.84), and culture-related conditions exhibit high informant consensus factors. Remedy preparation involves various plant parts, predominantly leaves (47.3%), followed by roots (22.1%), fruits (7.0%), and seeds (5.8%). Freshly harvested plant parts were frequently used (58.2%), while 24.7% involved both dried and fresh parts. Oral application (47.3%) and topical use (31.8%) are the major routes of remedy administration. The marketability of medicinal plants was evident, with 16.4% of the species reported as marketable, including Terminalia brownii Fresen. Myrtus communis L., Ruta chalepensis L., Olea europaea L. subsp. cuspidata (Wall. & G.Don) Cif., Allium sativum L. and Capsicum annuum L. Multipurpose plants such as Solanum somalense Franchet. (91.3% FL), Ocimum lamiifolium Hochst. ex. Benth. (88.9% FL), and Verbascum sinaiticum Benth. (85.7% FL) exhibited notable healing potentials. CONCLUSION: The current study underscores the intricate relationship between the local community and medicinal plants, emphasizing the importance of biodiversity conservation and health care and acknowledging the dynamic interplay between cultural heritage and ecosystem health. The results contribute to the development of sustainable conservation strategies, healthcare practices and the preservation of traditional knowledge, and highlight the interdependence of human societies and their natural environments. Community-based conservation initiatives with active participation of local communities are desirable for the conservation and sustainable use of medicinal plant species and their habitats. Raising public awareness about the sustainable harvesting and utilization of marketed medicinal plants (e.g., Terminalia brownii Fresen.) that are under threat is also important to ensure their availability for future generations and contribution to socioeconomic development.

Ethnomedicine, antibacterial activity, antioxidant potential and phytochemical screening of selected medicinal plants in Dibatie district, Metekel zone, western Ethiopia.

BACKGROUND: Medicinal plants play a major role in the delivery of healthcare, particularly among the rural population of Ethiopia. Plant extracts and their bioactive compounds have been utilized for the treatment of several diseases. This study was aimed at evaluating the antibacterial activity, antioxidant capacity, and phytochemical content of selected medicinal plants used in Dibatie district, western Ethiopia. METHODS: Study plants were collected, shade dried, pulverized, extracted by maceration in 80% ethanol, and subjected to antibacterial, antioxidant, and phytochemical tests. Minimum inhibitory concentration (MIC) was determined using 96-well microplates and nutrient broth microdilution. Antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay. Phytochemical screening was conducted using standard test methods. RESULTS: The ethanolic extract of Polystachya steudneri Rchb.f. pseudobulbs was the most active against gram-negative Proteus mirabilis, Salmonella typhimurium, Klebsiella pneumoniae, Escherichia coli, and Shigella flexneri, with MIC values of 8 ± 0, 11 ± 5, 3 ± 1, 3 ± 1, and 2 ± 0 mg/mL, respectively. The ethanolic extract of P. steudneri was also the most effective against gram-positive Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus agalactiae, and Enterococcus faecalis, with MIC values of 8 ± 0, 8 ± 0, 3 ± 1, and 16 ± 0 mg/mL, respectively. Ethanolic extracts of Gnidia involucrata Steud. ex A.Rich. stems and roots were effective antioxidants, with respective 50% DPPH free radical inhibitory concentrations (IC50) of 168.68 and 181.79 µg/mL, followed by that of P. steudneri (IC50 = 203.11 µg/mL). The study plants contained alkaloids, anthocyanins, anthraquinones, cardiac glycosides, coumarins, flavonoids, phenols, saponins, steroids, tannins, and terpenoids. CONCLUSIONS: This study confirmed the antibiotic, antioxidant, and phytochemical constituents of the investigated plants and suggested further investigations that may lead to bioactive lead compounds.

Antibacterial activity and phytochemical screening of traditional medicinal plants most preferred for treating infectious diseases in Habru District, North Wollo Zone, Amhara Region, Ethiopia.

Ethiopia's healthcare system relies on traditional medicinal practices that use medicinal plants to treat human and livestock ailments. However, the lack of empirical validation regarding the efficacy of these treatments against specific infectious diseases necessitates rigorous scientific investigations. The objective of this study was to investigate the antibacterial activity and phytochemical screening on five selected medicinal plant species, namely Solanum somalense Franchet., Verbascum sinaiticum Benth., Rumex nervosus Vahl, Withania somnifera (L.) Dunal and Calpurnia aurea (Ait.) Benth. The plants were first identified jointly with local informants and later considering mainly their high informant consensus and fidelity level values for their efficacy in treating infectious diseases in the area. Ethanol and aqueous extracts were prepared from the plant materials, and their antibacterial activities were evaluated against standard bacterial strains, representing both gram-positive and gram-negative types. To assess the antibacterial activity of the extracts, the minimum inhibitory concentration (MIC) was determined using the broth dilution method. Additionally, phytochemical screening was performed using standard qualitative tests to identify various secondary metabolites. The results indicated antibacterial efficacy in the ethanol extracts of S. somalense, W. somnifera, and C. aurea against particular bacterial strains (S. somalense against S. agalactiae with MIC of 1.5 mg/mL; W. somnifera against S. aureus and E. coli, with MIC of 2 mg/mL; C. aurea against E. coli and K. pneumoniae, with MICs of 3 mg/mL and 3.5 mg/mL, respectively). The results of the phytochemical screening indicated the presence of steroids, alkaloids, flavonoids, saponins, and terpenoids. The selected medicinal plants demonstrated promising antibacterial activity against certain bacterial strains. The current findings support the long-standing claim of the traditional medical system of the study area for their continued use of these plants in their treatment of infectious diseases. Further investigation is required to isolate the responsible active compounds and characterize the constituents and description of their antibacterial effect for possible use in areas where these infectious diseases are major health problems.

Ethnobotanical study of underutilized wild edible plants and threats to their long-term existence in Midakegn District, West Shewa Zone, Central Ethiopia.

BACKGROUND: Ethiopia is endowed with much plant diversity. The insignificant number of studies on wild edible plants with their ethnobotanical perspectives indicated that this plant diversity comprised only hundreds of wild edible plants used to supplement food sources for the local community under different conditions. There still need to be further investigations throughout the country when compared to the total area and cultural diversity of the country. However, they are seriously under pressure due to different natural and human influences. Therefore, the study was conducted to document underutilized wild edible plants along with their associated indigenous knowledge and explore threats to them in Midakegn District. METHODS: A questionnaire survey, semi-structured interviews, a market survey, score ranking, and focused group discussions were employed for data collection. Statistical analysis of ethnobotanical knowledge mean variation between different informant groups was computed by using one-way ANOVA in the IBM SPSS Statics version 24 package. RESULTS: A total of fifty underutilized wild edible plants belonging to 39 genera and 30 families were collected, recorded, and documented. The families Moraceae (four), Fabaceae, Flacourtaceae, Myrtaceae, Rosaceae, and Tiliaceae (each three) represented the highest number of species. It comprised shrubs (44%), trees (36%), herbs (18%), and epiphytes (2%). Fruits (62.3%) were found to be the most frequently used and mostly taken raw, fresh, or dried. These edible resources were consumed to supplement staple foods (67.3%), whereas 25% were used as emergency foods. The majority of species (96%) had multiple uses in addition to their edibility. A significantly higher (P < 0.05) number of underutilized wild edible plants were cited by males than females, by key informants than generals, elders than youngsters, illiterate than literate, and poorer than other wealth class groups of the community. Priority rankings indicate that agricultural expansion, fuel wood harvest, overgrazing, and selective harvesting are the most threatening factors to underutilized wild edible plants. CONCLUSIONS: Fifty underutilized wild edible plants, along with their associated indigenous knowledge, were recorded. Local people utilize them for supplementing staple food, as emergency food, to get relief, trust, and chew during drought. But they are mainly threatened by different human activities in the study area.

Ecological determinants in plant community structure across dry afromontane forest patches of Northwestern Ethiopia.

Ethiopia is a mountainous country with great geographic diversity. The diversified topographic features in Ethiopia made the country have a rich biodiversity forest cover in tropical Africa. This made Ethiopia have the largest floral diversity in tropical Africa. This floral diversity is rich in endemic elements. About 6,027 vascular plant species (including subspecies) with about 10.7% endemism have been documented. Plant community types are primarily influenced by topographic factors, as well as disturbance and environmental factors. The objective of this study is to demonstrate that 1: The forest patches in the study area exhibit distinct plant community types. 2: The composition and structure of these plant communities are influenced by various environmental variables. To achieve this, a total of 76 plots were used to collect vegetation and environmental data. The collected data were then analyzed using the R software, employing agglomerative hierarchical cluster analysis and redundancy analysis (RDA) to identify plant communities and assess the relationship between these communities and environmental variables.R software was used to identify plant communities and analyze the relationship between plant community types and environmental variables using agglomerative hierarchical cluster analysis and redundancy analysis (RDA). Four plant community types were identified. The RDA results highlighted the significant impact (p < 0.005) of altitude, aspect, slope, grazing, and human interference on species distribution and the formation of plant communities. The RDA results highlighted the significant impact (p < 0.005) of altitude, aspect, slope, grazing, and human interference on species distribution and the formation of plant communities.The findings indicate that the variation in plant communities is closely associated with topographic factors such as altitude, slope, aspect, as well as disturbance factors like grazing, and human interference, with altitude being the most influential factor. Based on these findings, it is recommended that conservation plans take into consideration the effects of grazing and human interference in order to address the challenges faced in conserving forest patches in the future.Additionally, further research efforts should focus on mitigating disturbance factors and understanding the environmental variables that affect forest patches to enhance their conservation.

Antibacterial and phytochemical analysis of traditional medicinal plants: An alternative therapeutic Approach to conventional antibiotics.

The purpose of this study was to carry out antibacterial and phytochemical analyses on six selected medicinal plants that have been traditionally used by the local people to treat and control different diseases. The antibacterial activities of methanolic extracts of these plants were assessed using the Agar well diffusion and Microtiter broth dilution methods. The root extract of Andrachne aspera showed significantly (p < 0.05) highest mean zone of inhibition at concentrations of 100 mg/ml (33 ± 0.17) and 200 mg/ml (33.5 ± 0.84) against S. epidermidis. The second highest mean zone of inhibition (24.8 ± 0.41) was recorded by Dichrostachys cinerea leaf extract against S. epidermidis at 200 mg/ml concentration. The minimum inhibitory concentrations 1.0 ± 0.0 was recorded by Andrachne aspera against E. faecalis and 2.0 ± 0.0 against S. aureus by Dichrostachys cinerea. The preliminary phytochemical analysis showed that Andrachne aspera and Dichrostachys cinerea contained strong concentration of Polyphenols and Flavonoids. Therefore, these two medicinal plant species have promising potential for further detailed investigations, including safety tests, characterization and isolation of bioactive secondary metabolites for the development of alternative drugs.

Composition of homegarden plants and cultural use in an indigenous community in Northwest Ethiopia.

BACKGROUND: Homegardens in Northern Ethiopia received little investigation into the diversity of plants and no study and recording in the Gozamin District. This study was used to fill the gap in how cultural use and cultural importance conserve species diversity in homegardens in the different agroclimatic zones in northwestern Ethiopia. METHODS: The study district and 12 kebeles were chosen using multistage and stratified random selection procedures based on traditional agroclimatic zones in the Gozamin District, Northwest Ethiopia, respectively. The number of plots chosen in each homegarden was determined by the homegarden's size, which ranges from 0.015 to 0.5 ha. These data were gathered by putting plots with a distance gradient from home (size: 10 × 10 m each). A semi-structured interview and complete plant inventory were conducted to document the informant's knowledge of plant species. Sørensen's similarity indices and Shannon-Wiener diversity indices were used to compare the similarity of sites and three agroclimatic zones, respectively. Direct matrix ranking, cultural importance (CI), the relative frequency of citation, and cultural value were used in quantitative analysis to compare the most common multipurpose plants. RESULTS: A total of 238 culturally important plant species from 81 families were identified. The Kruskal-Wallis test showed that there was a significant difference among the three agroclimatic zones species diversity (H = 103.4, Hc = 111.2, p < 0.05). Of the total plant species recorded, 59% were reported to be utilized for environmental uses, 35% were food crops, and 35% were medicinal plant species. The same was true for the three agroclimatic zones; food and medicinal uses were the first and second most important use categories, respectively. The similarity index for 64% of the sites investigated was less than 0.5. Cordia africana (FC = 125) was the most culturally significant species with a value of 2.23 on the CI index. CONCLUSION: Homegardens are multifunctional systems. The presence of different agroclimatic zones, cultural uses, cultural importance, and cultural value of the species are central to conserving plant species in the area. As the size of the garden increases, so does the diversity of species and uses. Our findings suggest that conservation strategies should take into account the links between plant composition and cultural importance.

Effects of essential oils on potato tuber sprouting at room temperature storage in Ethiopia.

This study was conducted with the objective of finding alternative management options for potato tuber sprouting during storage. Essential oils from Croton macrostachyus, Eucalyptus globulus, Allium sativum, Cymbopogon citratus, Cymbopogon martini, Rosmarinus officinalis and Thymus schimperi were applied in completely randomized design using two quantities (1 and 2 ml) and three frequencies (1, 2, 3). Essential oils from Cymbopogon citratus and Thymus schimperi showed the lowest percentage of weight loss relative to control tubers for varieties Gudene (8.07%) and Jalene (13.34%), respectively. Essential oils were applied in the form of vapour inside wooden box for 24 h without direct contact with tubers. For both varieties, the potato tuber weight loss found to be minimized using 2 ml essential oils. Similarly, lowest percentage of weight loss at one, two and three applications were found for variety Jalene from Cymbopogon martini, Eucalyptus globulus and Croton macrostachyus, respectively. On the other hand, lowest percentage of weight loss was maintained using Eucalyptus globulus for variety Gudene. The number and length of sprouts did not vary with quantities. Therefore, for variety Gudene, lower number of sprouts relative to control tuber (3 sprouts/tuber) was found on potato tuber treated with essential oils from Cymbopogon martini and Thymus schimperi (2.7 sprouts/tuber) whereas for variety Jalene, lower number of sprouts relative to control tuber (8.7 sprouts/tubers) was found on potato tubers treated with essential oils from Allium sativum and Rosmarinus officinalis (5.7 sprouts/tuber). The length of the longest sprouts on control tubers was 11.7 mm and 20 mm for varieties Gudene and Jalene, respectively. Longest and shortest length of sprout was found on potato tubers treated with Eucalyptus globulus and Rosmarinus officinalis, respectively, for both varieties. Therefore, essential oils used in the current study are promising alternatives to control potato sprouting during storage. However, seedlings of these species and other potential species must be made available to farmers with training on how to make extracts and apply them.

Ethnobotanical investigation of medicinal plants in Buska Mountain range, Hamar district, Southwestern Ethiopia.

BACKGROUND: Despite the fact that ethnobotanical studies have been conducted in various parts of Ethiopia, compared with the existence of the multitude and diverse ethnic groups and their associated traditional knowledge, the studies are not comprehensive enough for all the localities and communities in the country. This is also true for the Hamar community of Southwestern Ethiopia, who are totally dependent on plants and plant products for their livelihood. Hence, this investigation was done to identify and record medicine plants and the native wisdom of the community in the area. METHODS: Three hundred twenty six (326) informants were selected from the 12 lowest governmental units (Kebeles) applying Cochran's formula through stratified random sampling technique. From the total informants, 74 (48 males and 26 females) were purposively selected for in-depth discussion. Semi-structured interviews, focus group discussions, guided field walks as well as market surveys were used for data collection. Standard ethnobotanical analytical tools comprising ranking and comparison were used for the analysis. Preference ranking, pair-wise comparison, informant consensus factor, direct matrix ranking, Cultural Significance Index (CSI) and Jaccard's similarity coefficient (JCS) as well as Analysis of Variances (ANOVA), applying SPSS (version 20) were computed. RESULTS: A total of 145 species practical to cure about 72 ailments of livestock and humans were recorded. Families Fabaceae (with 22% of species), Asteraceae (11%), and Lamiaceae (9%) were discovered as the most dominant families in the area. Shrubs contributed the most (40%) to the growth forms followed by herbs (26.5%). Fresh leaves of the plants were parts that are used most frequently in the area. The highest ICF value (0.94) was recorded for reproductive problem categories. There was a relatively very high dependence of the community on plants and plant products together with a hoarded indigenous knowledge in the area that positively correlated with age (r = 0.82). CONCLUSION: The study's findings revealed that Buska Mountain range is a home for highly diverse and most dependable plant species and associated indigenous knowledge. However, because of the realized environmental threats in the area, the conservation efforts of the community should be invigorated and supported in order to sustain the biodiversity in general and the medicinal plant species in particular.

Ethnobotany of an indigenous tree Piliostigma thonningii (Schumach.) Milne-Redh. (Fabaceae) in the arid and semi-arid areas of South Omo Zone, southern Ethiopia.

BACKGROUND: Trees are important components of terrestrial ecosystems; they provide ecological, economic, and cultural services to humans. There is an urgent need for undertaking ethnobotanical investigations and documentation on the indigenous botanical knowledge of the local communities of a given area. This study was conducted to assess and document the categoric ethnobotany of Piliostigma thonningii and the associated indigenous knowledge of the local people related to use, management practices, and the threatening factors in the South Omo Zone of southwestern Ethiopia. MATERIALS AND METHODS: Six Kebeles were purposively selected from two districts of the zone and a total of 84 respondents were sampled, with consideration of gender, age, and wealth status. Data were collected using structured and semi-structured interviews, field observations, and group discussions. Relevant descriptive statistical methods were used to analyze the data. Ethnobotanical knowledge held by informants were computed using Pearson's chi-square test and direct matrix ranking and pair-wise ranking was used to prioritize the uses according to community preferences and the level of the destructiveness of the reported threats. RESULTS: The results showed that P. thonningii provides different functions to people's livelihoods as shade, food, fodder, soil fertility, fuelwood, medicine, rope, multipurpose materials (e.g., Borketa for sitting and head support), huts, beehives, farm implements, chairs, fences, and timber. The indigenous knowledge of local people on the uses of P. thonningii has shown significant (P < 0.05) relationship to age groups (being higher for elderly people). The consumption practice of respondents has significant (P < 0.05) association to the Kebeles, age, and income, with higher value for Hamer, lower aged groups, and low-income families. It provides nutritious animal feed to improve browse intake, survival, and productivity of domestic animals and also improve productivity of farm through amelioration of soil fertility. The status of P. thonningii has been decreasing due to agricultural expansion, collection of the species for firewood, dry fencing, and due to browsing. Respondents' attitude and interest to maintain and conserve the species has shown a significant (P< 0.05) association to gender, wealth, and level of education. Higher interest was observed in men than women, mid-high wealth class households than low income, and people with education than uneducated. CONCLUSIONS: This study attempted to provide information on the multiple uses of P. thonningii. Given the key roles of P. thonningii for the people and the environment to improve household food security, agricultural productivity, and income sources and the threats to it, the need to protect it in natural forests and woodlands and optimize its uses in agroforestry systems is high. Serious consideration of this species will ultimately allow households to reap the benefits expected to accrue from it in the arid and semi-arid areas of Ethiopia where plants of this nature are usually rare.

Ethnomycological study of edible and medicinal mushrooms in Menge District, Asossa Zone, Benshangul Gumuz Region, Ethiopia.

BACKGROUND: Menge District has long been inhabited by people who have a long tradition of using wild mushrooms mainly as food, source of income, and medicine. Extensive utilization of wild edible mushrooms (WEM) coupled with an ever-increasing population growth, deforestation, and agricultural land expansion threatens fungal diversity and WEM in the area. Hence, this study is aimed at documenting and analyzing the ethnomycological knowledge of the people in order to preserve the dwindling WEM wealth and associated indigenous knowledge. METHODS: Ethnomycological data were collected using semi-structured interviews, focus group discussions, participant observations, and walk-in-the-woods methods. Statistical tests were used to compare the indigenous knowledge and practice of wild mushroom among different informant categories using One-way ANOVA and t tests. RESULTS: A total of 20 ethnomycologically important wild mushroom species belonging to ten genera and six families were identified, of which 15 were reported to be edible in the District. The family Lyophyllaceae was represented by the highest number of species (nine species, 45%) followed by Agaricaceae (seven species, 35%) and each of the remaining four families had single species representation. Significant difference (P < 0.05) was observed on the mean number of WEM reported among different group of respondents. Wild edible mushroom collection habit and practice was significantly (P < 0.05) influenced by gender, age, and literacy level. The output of preference ranking exercise indicated Termitomyces schimperi was ranked first followed by Termitomyces letestui, Termitomyces microcarpus, and Termitomyces eurhizusas as the most preferred edible mushrooms respectively. CONCLUSION: The present study shows that Menge District is rich in wild mushroom diversity and associated indigenous knowledge. However, anthropogenic factors together with loss of indigenous knowledge and very poor conservation efforts threaten the survival of economically and ecologically important mushrooms in the area. Thus, complementary in situ and ex situ mushroom conservation strategy is highly recommended.

Evaluation of Skin Irritation and Acute and Subacute Oral Toxicity of Lavandula angustifolia Essential Oils in Rabbit and Mice.

Lavandula angustifolia is used in traditional and folk medicines of Ankober District, North Central Ethiopia, for the treatment of several livestock and human disorders. This toxicity study aimed to investigate L. angustifolia essential oil oral toxicity in mice and skin irritation in rabbit. L. angustifolia essential oil was analyzed using gas chromatography-mass spectrometry methods and showed predominance of Eucalyptol (52.36%), Camphor (11.91%), gamma-terpinene (8.775%) and endoborneol (7.585%). Limit test at 2000 mg/kg dose was used for L. angustifolia essential oil acute toxicity test and revealed LD50 value was higher than 2000 mg/kg. For subacute toxicity study 2000mg/kg was given orally to each mouse for 21 days. The result demonstrated no significant changes (p > 0.05) in the body weights, and biochemical parameters, gross abnormalities, water, and food intake were observed. No macroscopic changes were seen in the histopathology analysis of kidneys and livers. For skin irritation test shaved rabbit skin was treated with 10% ointment formulation. Ointment of L. angustifolia oil did not affect mice skin. Generally, this toxicity study demonstrated that L. angustifolia essential oil is nontoxic.