Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 26
Filtrar
Mais filtros

Base de dados
País/Região como assunto
Tipo de documento
País de afiliação
Intervalo de ano de publicação
1.
J Sci Food Agric ; 99(3): 1020-1028, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30009387

RESUMO

BACKGROUND: Citrus fruits possess a high content of bioactive compounds whose changes during fruit maturation have not been studied in depth. Fruits were sampled from week 1, after fruit onset (7 days after flowering), to week 14. Volatile compounds isolated by headspace-solid-phase microextraction and polar extracts from all samples were analyzed by gas chromatography-mass spectrometry. RESULTS: The relative abundance of 107 identified metabolites allowed differences among samples at different stages of fruit growth to be established. Principal component analysis showed a clear discrimination among samples, and analysis of variance revealed significant differences in 94 out of the 107 metabolites. Among total volatiles, monoterpenes increased their relative abundance from 86% to 94% during fruit growth, d-limonene, γ-terpinene and ß-pinene being the most abundant; conversely, sesquiterpenes decreased from 11.5% to 2.8%, ß-bisabolene and α-bergamotene being the most concentrated. Sugars, in general, exhibited a gradual increase in abundance, reaching a maximum between weeks 9 and 12. Citric and malic acids, representing approximately 90% of the total identified carboxylic acids, reached a maximum concentration at commercial maturity (week 14). CONCLUSION: Of the 107 tentatively identified metabolites during Persian lime growth, sugars, carboxylic acids, and volatiles were those that experienced more significant changes and more clearly created differences among fruit growth stages. © 2018 Society of Chemical Industry.


Assuntos
Citrus/metabolismo , Frutas/química , Ácidos Carboxílicos/metabolismo , Citrus/crescimento & desenvolvimento , Frutas/crescimento & desenvolvimento , Cromatografia Gasosa-Espectrometria de Massas/métodos , Açúcares/metabolismo , Compostos Orgânicos Voláteis/metabolismo
2.
Electrophoresis ; 38(18): 2349-2360, 2017 09.
Artigo em Inglês | MEDLINE | ID: mdl-28560773

RESUMO

Black garlic is increasing its popularity in cuisine around the world; however, scant information exists on the composition of this processed product. In this study, polar compounds in fresh garlic and in samples taken at different times during the heat treatment process to obtain black garlic have been characterized by liquid chromatography coupled to tandem mass spectrometry in high resolution mode. Ninety-five compounds (mainly amino acids and metabolites, organosulfur compounds, and saccharides and derivatives) were tentatively identified in all the analysed samples and classified as a function of the family they belong to. Statistical analysis of the results allowed establishing that the major changes in garlic occur during the first days of treatment, and they mainly affect to the three representative families. The main pathways involved in the synthesis of the compounds affected by heat treatment, and their evolution during the process were studied.


Assuntos
Cromatografia Líquida/métodos , Alho/química , Alho/metabolismo , Extratos Vegetais/análise , Extratos Vegetais/química , Espectrometria de Massas em Tandem/métodos , Aminoácidos/análise , Aminoácidos/química , Carboidratos/análise , Carboidratos/química , Análise Discriminante , Fermentação , Temperatura Alta
3.
Anal Bioanal Chem ; 407(18): 5381-92, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-25935675

RESUMO

Sweat is one of the less employed biofluids for discovery of markers in spite of its increased application in medicine for detection of drugs or for diagnostic of cystic fibrosis. In this research, human sweat was used as clinical sample to develop a screening tool for lung cancer, which is the carcinogenic disease with the highest mortality rate owing to the advanced stage at which it is usually detected. In this context, a method based on the metabolite analysis of sweat to discriminate between patients with lung cancer versus smokers as control individuals is proposed. The capability of the metabolites identified in sweat to discriminate between both groups of individuals was studied and, among them, a trisaccharide phosphate presented the best independent performance in terms of the specificity/sensitivity pair (80 and 72.7%, respectively). Additionally, two panels of metabolites were configured using the PanelomiX tool as an attempt to reduce false negatives (at least 80% specificity) and false positives (at least 80% sensitivity). The first panel (80% specificity and 69% sensitivity) was composed by suberic acid, a tetrahexose, and a trihexose, while the second panel (69% specificity and 80% sensitivity) included nonanedioic acid, a trihexose, and the monoglyceride MG(22:2). Thus, the combination of the five metabolites led to a single panel providing 80% specificity and 79% sensitivity, reducing the false positive and negative rates to almost 20%. The method was validated by estimation of within-day and between-days variability of the quantitative analysis of the five metabolites.


Assuntos
Neoplasias Pulmonares/diagnóstico , Metabolômica/métodos , Suor/química , Espectrometria de Massas em Tandem/métodos , Idoso , Cromatografia Líquida , Estudos de Coortes , Feminino , Humanos , Neoplasias Pulmonares/química , Masculino , Pessoa de Meia-Idade , Análise Multivariada , Curva ROC
4.
Anal Chem ; 86(15): 7558-65, 2014 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-24992377

RESUMO

Liquid chromatography coupled to tandem mass spectrometry is one of the most widely used analytical platforms for profiling analysis in metabolomics. One weakness of untargeted metabolomic analysis, however, is the difficulty of identifying metabolites. In fact, the process typically involves mass-based searching of LC-MS and LC-MS/MS data and requires using MS/MS data for unequivocal identification. Current strategies use LC-MS analysis in the scan mode prior to acquiring MS/MS information about targeted metabolites or the "auto MS/MS" mode to fragment automatically the most intense precursor ions. Therefore, in both cases additional injections are required to obtain MS/MS data after data treatment to identify significant compounds whose signals are not so intense. Because an additional procedure is needed to enhance the fraction of metabolites with MS/MS data, in this work, the effectiveness of utilizing different MS/MS parameters across an analytical batch or repetitions of the same sample by using exclusion or inclusion criteria to select precursor ions is assessed. The procedure, known as "gas-phase fractionation (GPF)", was used here for untargeted analysis of serum. The joint use of four methods with a different mass range for selection of precursor ions each provided useful MS/MS information for at least 80% of all molecular entities detected in the MS scan replicates. By contrast, the conventional "auto MS/MS" mode of data acquisition provided MS/MS data for only 48-57% of entities and was therefore less effective toward identifying metabolites. The additional use of GPF improved the detection and annotation of metabolite families such as phospholipids, amino acids, bile acids, carnitines, and fatty acids and their derivatives.


Assuntos
Cromatografia Líquida/métodos , Metabolômica , Espectrometria de Massas em Tandem/métodos , Análise Química do Sangue , Gases , Humanos
5.
Electrophoresis ; 35(17): 2509-19, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-24788107

RESUMO

The aim of this article is to provide an overview on the potential of the CE-herbicides binomial. To this end, the methods proposed so far are discussed: their characteristics, types of samples and analytes to which the methods have been applied, sample preparation steps, if required (e.g. cleanup-preconcentration, derivatization steps), and type of detection in each case. Also, the methods are compared with counterparts based on LC, when appropriate. The role of MS detection in present and future analytical research in this field (both for identification and quantitation) are commented. The foreseeable and desirable trends in analysis of herbicides are also outlined in the light of the present trends in metabolomics as a way of knowing the pathways, the intermediate and final degradation products that can influence the crops and the food chain of humans and other animals, as a result.


Assuntos
Eletroforese Capilar/métodos , Eletroforese Capilar/tendências , Herbicidas/análise , Herbicidas/química , Fracionamento Químico
6.
J Steroid Biochem Mol Biol ; 189: 101-107, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-30836177

RESUMO

The role of vitamin D in mammographic density is still unclear. This study examines the association between serum 25-hydroxyvitamin D (25(OH)D) and mammographic density, overall and by specific women characteristics. DDM-Madrid is a cross-sectional study that recruited 1403 premenopausal women in a breast radiodiagnosis unit of Madrid City Council. Information was collected with a questionnaire and plasma 25(OH)D was measured by solid-phase extraction on-line coupled to liquid chromatography-tandem mass spectrometry. Percent mammographic density was assessed using a semi-automated computer tool (DM-Scan). Multivariable linear regression models were used to quantify the associations, categorizing 25(OH)D levels (nmol/L) into 3 groups according to the cut-offs established by the US Endocrine Society. Models were adjusted for age, education, body mass index, age at menarche, parity, previous breast biopsies, family history of breast cancer, physical activity, energy intake, use of corticoids, hypercholesterolemia and day of sample extraction. Mean serum 25(OH)D level was 49.4 + 18.9 nmol/L. Women with sufficient concentrations of 25(OH)D showed a slight decrease in mammographic density (ß >75nmol/L=-3.40; p = 0.037). No differences were observed according to women characteristics except for parity, where the protective effect of 25(OH)D was only seen among nulliparous (ß >75nmol/L=-13.00; p-heterogeneity = 0.006). In light of the protective effect of vitamin D on mammographic density and the high prevalence of vitamin D insufficiency in our population, improving these levels could be an effective measure for the prevention of health problems related to the lack of this essential vitamin.


Assuntos
Densidade da Mama , Mama/diagnóstico por imagem , Deficiência de Vitamina D/sangue , Vitamina D/análogos & derivados , Neoplasias da Mama/sangue , Neoplasias da Mama/diagnóstico por imagem , Neoplasias da Mama/epidemiologia , Estudos Transversais , Feminino , Humanos , Mamografia , Pessoa de Meia-Idade , Pré-Menopausa/sangue , Espanha/epidemiologia , Vitamina D/sangue , Deficiência de Vitamina D/epidemiologia
7.
J Agric Food Chem ; 66(8): 1813-1820, 2018 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-29400054

RESUMO

Citrus fruits possess a high content of phenolic compounds; however, few studies have focused on the changes occurring during fruit growth. In this study, the changes in the concentration of 20 flavonoids, 4 phenolic acids, and their biosynthetic precursors phenylalanine and tyrosine have been evaluated during fruit maturation (14 weeks). Extracts from all samples, obtained by ultrasound assistance, were analyzed by liquid chromatography coupled to tandem mass spectrometry with a triple quad system (LC-QqQ MS/MS). In general, the concentration of flavanones, which represented over 70% of the studied phenols, and flavones increased during fruit growth, reaching their maximum concentration around week 12. In general, flavanols and phenolic acids exhibited their maximum concentration at week 5 and then decreasing significantly during the rest of maturation. Phenylalanine and tyrosine showed a sinuous behavior during fruit growth. Partial least-squares showed a clear differentiation among fruits belonging to different maturation stages, coumaric acid derivatives being the most influential variables on the projection.


Assuntos
Citrus/química , Frutas/crescimento & desenvolvimento , Fenóis/química , Extratos Vegetais/química , Cromatografia Líquida de Alta Pressão , Citrus/crescimento & desenvolvimento , Flavonoides/química , Frutas/química , Espectrometria de Massas
8.
J Pharm Biomed Anal ; 136: 22-31, 2017 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-28063332

RESUMO

The literature about the influence of vitamin D on multiple sclerosis (MS) is very controversial, possibly as a result of the way through which the research on the subject has been conducted. The studies developed so far have been focused exclusively on gene expression: the effect of a given vitamin D metabolite on target receptors. The influence of the vitamin D status (either natural or after supplementation) on MS has been studied by measurement of the 25 monohydroxylated metabolite (also known as circulating form), despite the 1,25 dihydroxylated metabolite is considered the active form. In the light of the multiple metabolic pathways in which both forms of vitamin D (D2 and D3) are involved, monitoring of the metabolites is crucial to know the activity of the target enzymes as a function of both the state of the MS patient and the clinical treatment applied. The study of metabolomics aspects is here proposed to clarify the present controversy. In "omics" terms, our proposal is to take profit from up-stream information-thus is, from metabolomics to genomics-with a potential subsequent step to systems biology, if required.


Assuntos
Metaboloma , Metabolômica , Esclerose Múltipla/tratamento farmacológico , Vitamina D/metabolismo , Vitamina D/uso terapêutico , Animais , Suplementos Nutricionais , Relação Dose-Resposta a Droga , Humanos , Metaboloma/genética , Esclerose Múltipla/metabolismo , Vitamina D/administração & dosagem
9.
J Agric Food Chem ; 54(23): 8775-81, 2006 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-17090121

RESUMO

A study of the nonvolatile fraction of extracts from vine shoots obtained by superheated ethanol-water mixtures is presented. The influence of the temperature, extraction time, and percentage of ethanol on extraction was investigated by a multivariate experimental design to maximize the yield of total phenolic compounds, measured by using the Folin-Ciocalteu method. The best values found for these variables were 80% (v/v) ethanol, 240 degrees C, and 60 min. Under these conditions, the effect of pH was also investigated, and a strong improvement of yield was observed by decreasing the pH. The extracts were subject to liquid-liquid extraction with n-hexane. The remaining polar phase was dried in a rotary evaporator and then reconstituted in 10 mL of water. The insoluble residue was dissolved in 10 mL of methanol. Both fractions (aqueous and methanolic) were analyzed by HPLC, and the differences in composition according to the extraction conditions were studied. Compounds usually present in commercial wood extracts were identified (mainly benzoic and hydroxycinnamic acids and aldehydes); the most abundant were quantified, and the stability of the identified phenolic families under different extraction conditions was also investigated. Finally, the superiority of the superheated liquid extraction over conventional solid-liquid extraction was demonstrated.


Assuntos
Flavonoides/isolamento & purificação , Fenóis/isolamento & purificação , Vitis/química , Cromatografia Líquida de Alta Pressão , Cromatografia com Fluido Supercrítico/métodos , Etanol/química , Flavonoides/análise , Cinética , Fenóis/análise , Brotos de Planta/química , Polifenóis , Temperatura , Água/química
10.
PLoS One ; 11(1): e0148056, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26807979

RESUMO

BACKGROUND: Flavonoids have shown to exert multiple beneficial effects on human health, being also appreciated by both food and pharmaceutical industries. Citrus fruits are a key source of flavonoids, thus promoting studies to obtain them. Characteristics of these studies are the discrepancies among sample pretreatments and among extraction methods, and also the scant number of comparative studies developed so far. OBJECTIVE: Evaluate the effect of both the sample pretreatment and the extraction method on the profile of flavonoids isolated from lemon. RESULTS: Extracts from fresh, lyophilized and air-dried samples obtained by shaking extraction (SE), ultrasound-assisted extraction (USAE), microwave-assisted extraction (MAE) and superheated liquid extraction (SHLE) were analyzed by LC-QTOF MS/MS, and 32 flavonoids were tentatively identified using MS/MS information. ANOVA applied to the data from fresh and dehydrated samples and from extraction by the different methods revealed that 26 and 32 flavonoids, respectively, were significant (p≤0.01). The pairwise comparison (Tukey HSD; p≤0.01) showed that lyophilized samples are more different from fresh samples than from air-dried samples; also, principal component analysis (PCA) showed a clear discrimination among sample pretreatment strategies and suggested that such differences are mainly created by the abundance of major flavonoids. On the other hand, pairwise comparison of extraction methods revealed that USAE and MAE provided quite similar extracts, being SHLE extracts different from the other two. In this case, PCA showed a clear discrimination among extraction methods, and their position in the scores plot suggests a lower abundance of flavonoids in the extracts from SHLE. In the two PCA the loadings plots revealed a trend to forming groups according to flavonoid aglycones. CONCLUSIONS: The present study shows clear discrimination caused by both sample pretreatments and extraction methods. Under the studied conditions, liophilization provides extracts with higher amounts of flavonoids, and USAE is the best method for isolation of these compounds, followed by MAE and SE. On the contrary, the SHLE method was the less favorable to extract flavonoids from citrus owing to degradation.


Assuntos
Técnicas de Química Analítica/métodos , Citrus/química , Flavonoides/análise , Extratos Vegetais/análise , Fracionamento Químico , Cromatografia Líquida , Espectrometria de Massas em Tandem
11.
Talanta ; 153: 386-91, 2016 06 01.
Artigo em Inglês | MEDLINE | ID: mdl-27130132

RESUMO

A study on the key role of lemon sample pretreatment on the analytical results is here presented. The objective of the study was to analyze the differences between extracts obtained from lyophilized and air-dried samples-the most common sample pretreatment in citrus studies-in comparison to extracts from fresh samples. All the extracts were obtained with ultrasound assistance and analyzed by LC-QTOF MS/MS. The dataset, constituted by 74 tentative identified metabolites, was first evaluated by ANOVA, which showed significant differences in the concentration of 44 out of 74 metabolites (p≤0.01). Also, the pairwise mean comparison (Tukey HSD; p≤0.01) revealed that the concentration of metabolites in the extracts from fresh and air-dried samples was quite similar and differed from that in lyophilized samples. On the other hand, application of principal component analysis (PCA) showed a clear discrimination between pretreatments, explaining 86.20% of the total variability. The results of this study suggest that the main differences between extracts could be attributed to the effect of freezing or heating on metabolic pathways, and not only to thermolability of the compounds.


Assuntos
Citrus , Cromatografia Líquida , Espectrometria de Massas em Tandem
12.
Talanta ; 144: 349-55, 2015 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-26452832

RESUMO

Peel, a part of the citrus rich in compounds with high-added value, constitutes the bulk of the waste generated in citrus juice industries. Flavonoids are a class of these high-added value compounds characterized by their bioactivity. In this research, a method for analysis of flavonoids, based on LC-MS/MS by using a triple quadrupole detector, has been developed and applied to the quantitative analysis of 16 flavonoids in extracts obtained by maceration of citrus peel. The parameters involved in the ionization and fragmentation of the target analytes were optimized to develop a selected reaction monitoring (SRM) method, which reported detection and quantitation limits ranging from 0.005 to 5 ng/mL and from 0.01 to 10 ng/mL, respectively. The raw materials for flavonoids extraction were fresh, oven-dried and lyophilized peel of 8 different orange varieties, and the proposed quantitation method was applied to the analysis of the obtained extracts. Evaluation of the two methods of water removal showed that lyophilization preserves the concentration of the flavonoids, while oven-dried peel presented a decrease of glycosylated flavonoids and an increase of aglycone forms.


Assuntos
Métodos Analíticos de Preparação de Amostras/métodos , Citrus sinensis/química , Flavonoides/análise , Calibragem , Cromatografia Líquida , Dessecação , Flavonoides/química , Flavonoides/isolamento & purificação , Cinética , Limite de Detecção , Modelos Lineares , Espectrometria de Massas em Tandem
13.
Talanta ; 141: 150-7, 2015 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-25966395

RESUMO

An optimized method for extraction and characterization of compounds present in orange peel has been developed. The extraction method allows the simultaneous extraction of polar and non-polar compounds by using two immiscible extractants (a polar extractant-an 80:20 (v/v) methanol-water mixture, and a non-polar extractant-n-hexane). The method is ultrasound-assisted, thus facilitating both formation of a stable emulsion between the two immiscible extractants and favoring mass-transfer from the solid sample to the liquid phases by a wide contact surface. Optimization of the ultrasound-assisted emulsification-extraction (USAEE) led to the following values as desirability conditions for both extracts: 32% amplitude, 0.75s/s duty cycle and 7.5min of extraction time. The extracts obtained under these conditions were analyzed by LC-QTOF MS/MS in positive and negative ionization modes. Tentative identification of the most significant compounds present in each extract allowed their characterization by using high resolution tandem mass spectrometry. The optimum extracts provided by USAEE were compared by using Principal Component Analysis to those obtained by conventional extraction based on maceration. Thus, the composition of the polar extracts obtained after 7.5min ultrasonication was similar to that of conventional maceration for 4h in both the ionization modes. On the contrary, the analysis of non-polar extracts led to different results depending on the ionization mode: the ultrasound-assisted extract was similar to those of conventional maceration for 10h in negative and positive ionization. This behavior could be explained by the contribution of different groups of compounds to each ionization mode.


Assuntos
Fracionamento Químico/métodos , Citrus sinensis/metabolismo , Espectrometria de Massas em Tandem/métodos , Ultrassom/métodos , Cromatografia Líquida , Citrus sinensis/química , Cumarínicos/análise , Emulsões/química , Ácidos Graxos/análise , Flavonoides/análise , Hexanos/química , Metanol/química , Extratos Vegetais/análise , Extratos Vegetais/química , Análise de Componente Principal
14.
J AOAC Int ; 86(2): 394-9, 2003.
Artigo em Inglês | MEDLINE | ID: mdl-12723923

RESUMO

This paper reviews the use of analytical pervaporation (defined as the integration of 2 different analytical separation principles, evaporation and gas diffusion, in a single micromodule) coupled to flow-injection manifolds for the determination of analytes of interest in enology; the review discusses the advantages that these techniques can provide in wine analytical laboratories. Special attention is given to methods that enable the determination of either of 2 volatile analytes, or of one volatile analyte and one nonvolatile analyte by taking advantage of the versatility of the designed approaches. In a comparison of these methods with the official and/or standard methods, the results showed good agreement. In addition, the new methods offer improvements in linear determination range, quantitation limit, precision, rapidity, and potential for full automation. Thus, this review demonstrates that although the old technologies used in wine analytical laboratories may be supported by official and standard methods, they should be replaced by properly validated, new, and automated technologies.


Assuntos
Análise de Alimentos/instrumentação , Vinho/análise , Cromatografia Gasosa , Difusão , Análise de Alimentos/métodos , Volatilização
15.
Food Chem ; 136(2): 576-84, 2013 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-23122100

RESUMO

The effect of breakfast intake of fried oils containing natural antioxidants or a synthetic autooxidation inhibitor on the metabolism of essential fatty acids focused on obese individuals. Serum levels of eicosanoids were compared in individuals before and after intake of different breakfasts. Univariate descriptive analysis was used to characterise the cohort selected for this study and multivariate analysis to reveal statistical differences of normalised eicosanoids concentrations (determined by solid-phase extraction coupled to LC-MS/MS) depending on the edible oil used for breakfast preparation. The results showed that the intake of breakfast prepared with pure sunflower oil subjected to deep frying causes an effect over the eicosanoids profile that enables discrimination versus the rest of individuals. The effect was a significant increase in the concentration of hydroxyoctadecadienoic acid (HODE) metabolites, indicative markers of the intake of fried oils. The concentration of HODE metabolites was lower when the oil contained either natural antioxidants from olive-oil pomace or a synthetic autooxidation inhibitor as dimethylsiloxane. The comparison of the effect of fried sunflower oils with fried extra virgin olive oil shows the benefits associated to the consumption of the latter.


Assuntos
Ácidos Graxos Essenciais/metabolismo , Obesidade/dietoterapia , Óleos de Plantas/administração & dosagem , Adulto , Idoso , Estudos de Coortes , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Obesidade/metabolismo , Azeite de Oliva , Óleos de Plantas/metabolismo , Óleo de Girassol , Fatores de Tempo
16.
Food Chem ; 141(3): 1722-31, 2013 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-23870884

RESUMO

The metabolic profile of human serum after intake of breakfasts prepared with different heated vegetable oils has been studied. Four oils (olive and sunflower oils, pure and enriched with natural and artificial oxidation inhibitors) were subjected to a simulated heated process prior to breakfast preparation. A metabolomics global profiling approach performed on post-basal serum samples revealed statistical differences among individuals based on breakfast intake, and identified compounds responsible for such differences. Serum samples obtained in basal state (control samples) and 2 and 4h after programmed intakes were analyzed by LC-TOF/MS. The resulting fingerprints were compared and differences between basal and post-basal states evaluated, observing that the intake of different breakfasts altered the metabolic signature of serum. Analysis models based on PLS algorithms were developed to discriminate individuals in post-basal state for each intervention breakfast. Then, Volcano tests enabled to detect significant molecular entities explaining the variability associated to each breakfast. It is worth emphasizing the importance of fatty acids, their derivatives and phospholipids for tentative identification.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Espectrometria de Massas/métodos , Metabolômica/métodos , Obesidade/sangue , Óleos de Plantas/metabolismo , Soro/química , Adulto , Idoso , Desjejum , Culinária , Feminino , Temperatura Alta , Humanos , Masculino , Pessoa de Meia-Idade , Obesidade/metabolismo , Obesidade/fisiopatologia , Óleos de Plantas/química
17.
Food Chem ; 138(4): 2250-9, 2013 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-23497883

RESUMO

We have investigated the effects of the intake of oils heated at frying temperature in order to find an oil model for deep-frying that prevents postprandial oxidative stress. Twenty obese people received four breakfasts following a randomised crossover design consisting of different oils (virgin olive oil (VOO), sunflower oil (SFO), and a mixed seed oil (SFO/canola oil) with added dimethylpolysiloxane (SOX) or natural antioxidants from olives (SOP)), which were subjected to 20 heating cycles. The intake of SFO-breakfast reduced plasma GSH levels and the GSH/GSSG ratio, increased protein carbonyl levels, and induced a higher gene expression of the different NADPH-oxidase subunits, Nrf2-Keap1 activation, gene expression of the antioxidant enzymes in peripheral blood mononuclear cells and antioxidant plasma activities than the intake of the breakfasts prepared with VOO, SOP and SOX. Oils with phenolic compounds, whether natural (VOO) or artificially added (SOP), or with artificial antioxidant (SOX), could reduce postprandial oxidative stress compared with sunflower oil.


Assuntos
Antioxidantes/metabolismo , Obesidade/dietoterapia , Obesidade/metabolismo , Estresse Oxidativo , Óleos de Plantas/metabolismo , Adulto , Idoso , Antioxidantes/química , Ácidos Graxos Monoinsaturados/química , Ácidos Graxos Monoinsaturados/metabolismo , Feminino , Aditivos Alimentares/metabolismo , Temperatura Alta , Humanos , Masculino , Pessoa de Meia-Idade , Azeite de Oliva , Oxirredução , Óleos de Plantas/química , Período Pós-Prandial , Óleo de Brassica napus , Óleo de Girassol
18.
J Plant Physiol ; 169(17): 1673-9, 2012 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-22841626

RESUMO

The physiological and biochemical bases for glyphosate resistance and susceptibility in horseweed (Conyza canadensis L. Cronq.) populations collected from Córdoba, Huelva, Málaga, Jaén and Seville in southern Spain were investigated. Screening 25 populations treated with glyphosate (238gacidequivalentha(-1)) at the rosette stage (BBCH 14-15) revealed reductions in fresh weight (fw) of 9-99%. The resistant biotype (R C004) was 6.1 times more resistant than the susceptible biotype (S). Shikimate accumulation in both biotypes increased until 72h after treatment (HAT), and then continued to increase (to 61.2%) in the S biotype, but decreased by 40% in the R (C004) biotype. Differential glyphosate spray retention and foliar uptake of applied (14)C-glyphosate between the R (C004) and S biotype had no effect on resistance to this herbicide. Quantitative and qualitative tests showed greater (14)C-glyphosate mobility in the S biotype than in the R (C004) biotype. Glyphosate was metabolized faster in the R (C004) biotype than in the S biotype. The herbicide disappeared completely from the R (C004) biotype by conversion into glyoxylate, sarcosine and aminomethylphosphonic acid within 96 HAT. On the other hand, 41.43nmolg(-1)fw of all glyphosate applied remained in the S biotype and glyoxylate was its only non-toxic metabolite. These results suggest that glyphosate resistance in horseweed is due to two different non-target mechanisms, namely: (a) impaired glyphosate translocation and (b) glyphosate metabolism to other compounds.


Assuntos
Conyza/efeitos dos fármacos , Glicina/análogos & derivados , Herbicidas/farmacologia , Isótopos de Carbono/metabolismo , Ácidos Carboxílicos/metabolismo , Conyza/genética , Conyza/metabolismo , Relação Dose-Resposta a Droga , Genótipo , Glicina/metabolismo , Glicina/farmacologia , Resistência a Herbicidas , Herbicidas/metabolismo , Espécies Introduzidas , Isoxazóis , Organofosfonatos/metabolismo , Folhas de Planta/efeitos dos fármacos , Folhas de Planta/genética , Folhas de Planta/metabolismo , Distribuição Aleatória , Sarcosina/metabolismo , Espanha , Espectrofotometria , Tetrazóis , Controle de Plantas Daninhas , Glifosato
19.
J Agric Food Chem ; 59(13): 7194-202, 2011 Jul 13.
Artigo em Inglês | MEDLINE | ID: mdl-21644588

RESUMO

The influence of deep frying, mimicked by 20 heating cycles at 180 °C (each cycle from ambient temperature to 180 °C maintained for 5 min), on the unsaponifiable fraction of vegetable edible oils represented by three characteristic families of compounds (namely, phytosterols, aliphatic alcohols, and triterpenic compounds) has been studied. The target oils were extra virgin olive oil (with intrinsic content of phenolic antioxidants), refined sunflower oil enriched with antioxidant phenolic compounds isolated from olive pomace, refined sunflower oil enriched with an autoxidation inhibitor (dimethylpolysiloxane), and refined sunflower oil without enrichment. Monitoring of the target analytes as a function of both heating cycle and the presence of natural antioxidants was also evaluated by comparison of the profiles after each heating cycle. Identification and quantitation of the target compounds were performed by gas cromatography-mass spectrometry in single ion monitoring mode. Analysis of the heated oils revealed that the addition of natural antioxidants could be an excellent strategy to decrease degradation of lipidic components of the unsaponifiable fraction with the consequent improvement of stability.


Assuntos
Antioxidantes/administração & dosagem , Temperatura Alta , Óleos de Plantas/química , Saponinas/química , Álcoois/química , Azeite de Oliva , Fenóis/administração & dosagem , Fitosteróis/química , Óleo de Girassol , Triterpenos/química
20.
J Agric Food Chem ; 58(13): 7539-46, 2010 Jul 14.
Artigo em Inglês | MEDLINE | ID: mdl-20550122

RESUMO

The determination of sterols and fatty alcohols is a part of the study of the metabolomic profile of the unsaponifiable fraction in olive tree. Leaves and drupes from three varieties of olive tree (Arbequina, Picual, and Manzanilla) were used. The content of the target compounds was studied in five ripeness stages and three harvesting periods for olive drupes and leaves, respectively. A method based on ultrasound-assisted extraction and derivatization for the individual identification and quantitation of sterols and fatty alcohols, involving chromatographic separation and mass spectrometry detection by selected ion monitoring, was used. The concentrations of alcohols and sterols in the drupes ranged between 0.1 and 1086.9 mug/g and between 0.1 and 5855.3 mug/g, respectively, which are higher than in leaves. Statistical studies were developed to show the relationship between the concentration of the target analytes and variety, ripeness stage, and harvesting period.


Assuntos
Álcoois Graxos/análise , Olea/química , Extratos Vegetais/análise , Esteróis/análise , Olea/crescimento & desenvolvimento
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA