Genome-wide identification and systematic analysis of the HD-Zip gene family and its roles in response to pH in Panax ginseng Meyer.

BACKGROUND: Ginseng, Panax ginseng Meyer, is a traditional herb that is immensely valuable both for human health and medicine and for medicinal plant research. The homeodomain leucine zipper (HD-Zip) gene family is a plant-specific transcription factor gene family indispensable in the regulation of plant growth and development and plant response to environmental stresses. RESULTS: We identified 117 HD-Zip transcripts from the transcriptome of ginseng cv. Damaya that is widely grown in Jilin, China where approximately 60% of the world's ginseng is produced. These transcripts were positioned to 64 loci in the ginseng genome and the ginseng HD-Zip genes were designated as PgHDZ genes. Identification of 82 and 83 PgHDZ genes from the ginseng acc. IR826 and cv. ChP genomes, respectively, indicated that the PgHDZ gene family consists of approximately 80 PgHDZ genes. Phylogenetic analysis showed that the gene family originated after Angiosperm split from Gymnosperm and before Dicots split from Monocots. The gene family was classified into four subfamilies and has dramatically diverged not only in gene structure and functionality but also in expression characteristics. Nevertheless, co-expression network analysis showed that the activities of the genes in the family remain significantly correlated, suggesting their functional correlation. Five hub PgHDZ genes were identified that might have central functions in ginseng biological processes and four of them were shown to be actively involved in plant response to environmental pH stress in ginseng. CONCLUSIONS: The PgHDZ gene family was identified from ginseng and analyzed systematically. Five potential hub genes were identified and four of them were shown to be involved in ginseng response to environmental pH stress. The results provide new insights into the characteristics, diversity, evolution, and functionality of the PgHDZ gene family in ginseng and lay a foundation for comprehensive research of the gene family in plants.

Experimental and simulation study on chatter stability region of integral impeller with non-uniform allowance.

In order to accurately improve and predict chatter stability region of machining process, an optimization method of machining process with non-uniform allowance of integral impeller was proposed. The modal parameters of the workpiece process system were obtained using the finite element analysis. Based on the regenerative chatter analysis theory, a limit comparison diagram of the stability with uniform allowance and non-uniform allowance was established. The simulation results showed that the non-uniform allowance natural frequency is about 1.43 times as much as the uniform allowance natural frequency, and the machining system stiffness non-uniform allowance is twice as much as the uniform allowance, while the limit of chatter stability region is increased by 3 times. This article studied uniform allowance and non-uniform allowance of milling chatter stability with experimental method. Tool path for five-axis machining and machine tool simulation based on NX CAM were planned. The comparisons of cutting processing uniform allowance and non-uniform allowance were done, and the surface profile detection of the test part with the three-dimensional scanning was carried out. The experimental results showed that the average optimization rate for manufacturing precision of blade suction surface after optimization and pressure surface was 63.8% and 48.84%. The total experiment showed that this process optimization strategy could effectively improve the stiffness of the integral impeller blade and reduce the cutting chatter of the blade during the cutting process.