Interplays between plants and environmental factors determine pullout resistance on different revegetated slopes in mining areas.

Plant roots play a crucial role in enhancing soil stability and protecting slopes during ecological restoration, particularly in mining areas where external-soil spray seeding is employed. However, the relationship between plant root pullout resistance and environmental factors on different types of slopes remains unclear. In this study, we investigated the interactions between the pullout resistance of a dominant species, Artemisia gmelinii, and environmental factors on three slope types (rocky, geotechnical, and soil) using multi-group structural equation modeling. Our findings reveal that the pullout resistance of plant roots was strongly influenced by various factors, including but not limited to biological factors such as plant height and biomass. It showed a positive correlation between soil silt content and soil nutrient levels. Notably, the pullout resistance on soil slopes was significantly higher than on rocky slopes. Furthermore, the impact of soil nutrients and texture on pullout resistance was more pronounced on geotechnical and soil slopes compared to rocky slopes. Multi-group structural equation modeling highlighted that among all environmental factors, slope gradient and underground biomass had the most significant influence on pullout resistance across all slope types. Specifically, slope gradient had a greater effect on soil slopes, whereas underground biomass played a more prominent role on rocky and geotechnical slopes. Overall, our study suggests that when implementing external-soil spray seeding in mining areas, it is crucial to consider the interplay between plant roots and environmental factors, including slope properties. This holistic approach is crucial for maximizing the effectiveness of plants in slope protection during eco-engineering projects.

The association of leaf lifespan and background insect herbivory at the interspecific level.

Herbivory is well known to be a major selective pressure that affects plant communities, but the leaf traits that mediate variations in herbivory at the interspecific level remain controversial. We collected published data on background insect herbivory and leaf traits from a wide variety of species to test the hypothesis that species with intermediate leaf lifespans, lower fiber, and higher nutrient contents in leaves should have higher levels of herbivory. We found that at the interspecific level herbivory had a hump-shaped relationship with leaf lifespan and a positive relationship with leaf size. Surprisingly, our data show that nutritional traits have little relationship to herbivory. Our study provides new insights relevant to the recent debate on leaf trait-herbivory relationships. These findings are especially helpful in explaining the general patterns of herbivory detected on the global scale.

Quantifying the characteristics of particulate matters captured by urban plants using an automatic approach.

It is widely accepted that urban plant leaves can capture airborne particles. Previous studies on the particle capture capacity of plant leaves have mostly focused on particle mass and/or size distribution. Fewer studies, however, have examined the particle density, and the size and shape characteristics of particles, which may have important implications for evaluating the particle capture efficiency of plants, and identifying the particle sources. In addition, the role of different vegetation types is as yet unclear. Here, we chose three species of different vegetation types, and firstly applied an object-based classification approach to automatically identify the particles from scanning electron microscope (SEM) micrographs. We then quantified the particle capture efficiency, and the major sources of particles were identified. We found (1) Rosa xanthina Lindl (shrub species) had greater retention efficiency than Broussonetia papyrifera (broadleaf species) and Pinus bungeana Zucc. (coniferous species), in terms of particle number and particle area cover. (2) 97.9% of the identified particles had diameter ≤10 µm, and 67.1% of them had diameter ≤2.5 µm. 89.8% of the particles had smooth boundaries, with 23.4% of them being nearly spherical. (3) 32.4%-74.1% of the particles were generated from bare soil and construction activities, and 15.5%-23.0% were mainly from vehicle exhaust and cooking fumes.

Effects of road dust on the growth characteristics of Sophora japonica L. seedlings.

Road dust is one of the most common pollutants and causes a series of negative effects on plant physiology. Dust's impacts on plants can be regarded as a combination of load, composition and grain size impacts on plants; however, there is a lack of integrated dust effect studies involving these three aspects. In our study, Sophora japonica seedlings were artificially dusted with road dust collected from the road surface of Beijing so that we could study the impacts of this dust on nitrogen/carbon allocation, biomass allocation and photosynthetic pigments from the three aspects of composition, load and grain size. The results showed that the growth characteristics of S. japonica seedlings were mostly influenced by dust composition and load. Leaf N, root-shoot ratio and chlorophyll a/b were significantly affected by dust composition and load; leaf C/N, shoot biomass, total chlorophyll and carotenoid were significantly affected by dust load; stem N and stem C/N were significantly affected by dust composition; while the dust grain size alone did not affect any of the growth characteristics. Road dust did influence the growth characteristics more extensively than loam. Therefore, a higher dust load could increase the differences between road dust and loam treatments. The elements in dust are well correlated to the shoot N, shoot C/N, and root-shoot ratio of S. japonica seedlings. This knowledge could benefit the management of urban green spaces.

Reconstruction of Population-Level Migration Trajectories of Black-Faced Spoonbill (Platalea minor) Based on Citizen Science Data.

Migration is a critical ecological process for birds. Understanding avian migratory routes is essential for identifying important stopover sites and key foraging areas to ensure high-quality stopovers for birds. The Black-faced Spoonbill (Platalea minor), a national Grade I protected wild animal in China, is classified as endangered on the IUCN Red List of Threatened Species. Studying the migratory routes of the Black-faced Spoonbill and identifying critical stopover sites across different life histories is vital for its conservation. However, research on the migratory routes of this species has been very limited. This study, utilizing citizen science data and the Level-order-Minimum-cost-Traversal (LoMcT) algorithm, reconstructs the migratory trajectories of the Black-faced Spoonbill from 2018 to 2022. The results show that Wenzhou, Xiamen, Shantou, Shanwei, Hsinchu, Chiayi, and Tainan are significant stopovers for this species. The Black-faced Spoonbill is actively migratory during the migration season across the southeastern coastal region of China. The simulation results of this study reveal the migratory routes and activity patterns of the Black-faced Spoonbill, providing critical support for its conservation.

Artificial light at night decreases leaf herbivory in typical urban areas.

Artificial light at night (ALAN) is exerting growing pressure on natural ecosystems, but its impact on biological interactions remains unclear. This study aimed to assess how ALAN influences leaf functional traits and herbivory in two prevalent street tree species (Styphnolobium japonicum (L.) Schott and Fraxinus pennsylvanica) through field surveys and paired experiments in the urban areas of Beijing, China. We found that ALAN led to increased leaf toughness and decreased levels of leaf herbivory. Additionally, ALAN showed species-specific effects on leaf nutrients, size as well as defense substances. The findings illustrate that ALAN can significantly alter some key functional traits and ecological processes (nutrient cycling, energy flow). In general, we suggest that high ALAN intensity will be detrimental to the energy flow from urban plants to higher trophic levels, posing a potential threat to the maintenance of biodiversity (e.g., arthropod diversity, bird diversity) in urban ecosystems.

Spatial Distribution and Sustainable Development of Living Woody and Coarse Woody Debris in Warm-Temperate Deciduous Broadleaved Secondary Forests in China.

The investigation into the spatial patterns of living woody (LWD) and coarse woody debris (CWD) in warm-temperate deciduous broadleaved secondary forests serves as a foundational exploration of the mechanisms governing coexistence and mortality in forest ecosystems. The complete spatial randomness null model (CSR) was employed to analyze spatial distribution patterns, with the independent component null model (IC) and canonical correspondence analysis (CCA) utilized to elucidate spatial correlations and topographic influences. All three models were applied to LWD and CWD across various size classes within a 20-hectare plot in the Dongling Mountains. The study's findings indicate that both LWD and CWD predominantly exhibited aggregated patterns, transitioning to a random distribution as the size class increased. Both increasing abundance and maximum diameter at breast height (DBH) also have a significant influence on the distribution of species. Notably, rare species exhibited higher aggregation compared to common and abundant species. The spatial correlation results between LWD and CWD across various size classes predominantly showed positive correlations and uncorrelated patterns within the sampled plots. CCA analysis further revealed that elevation, convexity, slope, and aspect significantly influenced the spatial patterns of LWD and CWD across different size classes. Within the sample site, trees display a tendency to grow and die in clusters. Biotic factors have a more significant influence on species distribution than abiotic factors.

A division-of-labor mode contributes to the cardioprotective potential of mesenchymal stem/stromal cells in heart failure post myocardial infarction.

Background: Treatment of heart failure post myocardial infarction (post-MI HF) with mesenchymal stem/stromal cells (MSCs) holds great promise. Nevertheless, 2-dimensional (2D) GMP-grade MSCs from different labs and donor sources have different therapeutic efficacy and still in a low yield. Therefore, it is crucial to increase the production and find novel ways to assess the therapeutic efficacy of MSCs. Materials and methods: hUC-MSCs were cultured in 3-dimensional (3D) expansion system for obtaining enough cells for clinical use, named as 3D MSCs. A post-MI HF mouse model was employed to conduct in vivo and in vitro experiments. Single-cell and bulk RNA-seq analyses were performed on 3D MSCs. A total of 125 combination algorithms were leveraged to screen for core ligand genes. Shinyapp and shinycell workflows were used for deploying web-server. Result: 3D GMP-grade MSCs can significantly and stably reduce the extent of post-MI HF. To understand the stable potential cardioprotective mechanism, scRNA-seq revealed the heterogeneity and division-of-labor mode of 3D MSCs at the cellular level. Specifically, scissor phenotypic analysis identified a reported wound-healing CD142+ MSCs subpopulation that is also associated with cardiac protection ability and CD142- MSCs that is in proliferative state, contributing to the cardioprotective function and self-renewal, respectively. Differential expression analysis was conducted on CD142+ MSCs and CD142- MSCs and the differentially expressed ligand-related model was achieved by employing 125 combination algorithms. The present study developed a machine learning predictive model based on 13 ligands. Further analysis using CellChat demonstrated that CD142+ MSCs have a stronger secretion capacity compared to CD142- MSCs and Flow cytometry sorting of the CD142+ MSCs and qRT-PCR validation confirmed the significant upregulation of these 13 ligand factors in CD142+ MSCs. Conclusion: Clinical GMP-grade 3D MSCs could serve as a stable cardioprotective cell product. Using scissor analysis on scRNA-seq data, we have clarified the potential functional and proliferative subpopulation, which cooperatively contributed to self-renewal and functional maintenance for 3D MSCs, named as "division of labor" mode of MSCs. Moreover, a ligand model was robustly developed for predicting the secretory efficacy of MSCs. A user-friendly web-server and a predictive model were constructed and available (https://wangxc.shinyapps.io/3D_MSCs/).

High rodent abundance increases seed removal but decreases scatter-hoarding and seedling recruitment along an elevational gradient.

The distributions of small rodents in mountainous environments across different elevations can provide important information regarding the effects of climate change on the dispersal of plant species. However, few studies of oak forest ecosystems have compared the elevational patterns of sympatric rodent diversity, seed dispersal, seed bank, and seedling abundance. Thus, we tested the differences in the seed disperser composition and abundance, seed dispersal, seed bank abundance, and seedling recruitment for Quercus wutaishanica along 10 elevation levels in the Taihang Mountains, China. Our results provide strong evidence that complex asymmetric seed dispersal and seedling regeneration exist along an elevational gradient. The abundance of rodents had a significant negative correlation with the elevation and the seed removal rates peaked and then declined with increasing elevation. The seed removal rates were higher at middle and lower elevations than higher elevations but acorns were predated by 5 species of seed predators at middle and lower elevations, and thus, there was a lower likelihood of recruitment compared with those dropped beneath mother oaks at higher elevations. More importantly, the number of individual seeds in the seed bank and seedlings increased with the elevation, although dispersal services were reduced at sites lacking rodents. As conditional mutualists, the rodents could possibly act as antagonistic seed predators rather than mutualistic seed dispersers at low and middle elevations, thereby resulting in the asymmetric pattern of rodent and seedling abundance with increasing elevation to affect the community assembly and ecosystem functions on a large spatial scale.

Metformin reduces hepatocarcinogenesis by inducing downregulation of Cyp26a1 and CD8+ T cells.

BACKGROUND: Hepatocellular carcinoma (HCC) is a highly heterogeneous cancer with major challenges in both prevention and therapy. Metformin, adenosine monophosphate-activated protein kinase (AMPK) activator, has been suggested to reduce the incidence of HCC when used for patients with diabetes in preclinical and clinical studies. However, the possible effects of metformin and their mechanisms of action in non-diabetic HCC have not been adequately investigated. METHODS: Fah-/-  mice were used to construct a liver-injury-induced non-diabetic HCC model for exploring hepatocarcinogenesis and therapeutic potential of metformin. Changes in relevant tumour and biochemical indicators were measured. Bulk and single-cell RNA-sequencing analyses were performed to validate the crucial role of proinflammatory/pro-tumour CD8+ T cells. In vitro and in vivo experiments were performed to confirm Cyp26a1-related antitumour mechanisms of metformin. RESULTS: RNA-sequencing analysis showed that chronic liver injury led to significant changes in AMPK-, glucose- and retinol metabolism-related pathways in Fah-/- mice. Metformin prevented the formation of non-diabetic HCC in Fah-/- mice with chronic liver injury. Cyp26a1 ddexpression in hepatocytes was significantly suppressed after metformin treatment. Moreover, downregulation of Cyp26a1 occurred in conjunction with increased levels of all-trans-retinoic acid (atRA), which is involved in the activation of metformin-suppressed hepatocarcinogenesis in Fah-/- mice. In contrast, both CD8+  T-cell infiltration and proinflammatory/pro-tumour cytokines in the liver were significantly upregulated in Fah-/- mice during chronic liver injury, which was notably reversed by either metformin or atRA treatment. Regarding mechanisms, metformin regulated the decrease in Cyp26a1 enzyme expression and increased atRA expression via the AMPK/STAT3/Gadd45ß/JNK/c-Jun pathway. CONCLUSIONS: Metformin inhibits non-diabetic HCC by upregulating atRA levels and downregulating CD8+ T cells. This is the first reporting that the traditional drug metformin regulates the metabolite atRA via the Cyp26a1-involved pathway. The present study provides a potential application of metformin and atRA in non-diabetic HCC.

Rodent-mediated plant seed dispersal: What happens to the seeds after entering the gaps with different sizes?

In general, it is accepted that gap formation significantly affects the placement of scatter-hoarded seeds by small rodents, but the effects of different forest gap sizes on the seed-eating and scatter-hoarding behaviors of small rodents remain unclear. Thus, we examined the effects of a closed-canopy forest, forest edge, and gaps with different sizes on the spatial dispersal of Quercus variabilis acorns and cache placement by small rodents using coded plastic tags in the Taihang Mountains, China. The seeds were removed rapidly, and there were significant differences in the seed-eating and caching strategies between the stand types. We found that Q. variabilis acorns were usually eaten after being removed from the closed-canopy forest and forest edges. By contrast, the Q. variabilis acorns in the forest gap stands were more likely to be scatter-hoarded. The dispersal distances of Q. variabilis acorns were significantly longer in the forest gap plots compared with the closed canopy and forest edge plots. However, the proportion of scatter-hoarded seeds did not increase significantly as the gap size increased. In small-scale oak reforestation projects or research, creating small gaps to promote rodent-mediated seed dispersal may effectively accelerate forest recovery and successional processes.

Identifying changing interspecific associations along gradients at multiple scales using wavelet correlation networks.

Identifying interspecific associations is very important for understanding the community assembly process. However, most methods provide only an average association and assume that the association strength does not vary along the environmental gradient or with time. The scale effects are generally ignored. We integrated the idea of wavelet and network topological analysis to provide a novel way to detect nonrandom species associations across scales and along gradients using continuous or presence-absence ecological data. We first used a simulated species distribution data set to illustrate how the wavelet correlation analysis builds an association matrix and demonstrates its statistical robustness. Then, we applied the wavelet correlation network to a presence-absence data set of soil invertebrates. We found that the associations of invertebrates varied along an altitudinal gradient. We conclude by discussing several possible extensions of this method, such as predicting community assembly, utility in the temporal dimension, and the shifting effects of highly connected species within a community. The combination of the multiscale decomposition of wavelet and network topology analysis has great potential for fostering an understanding of the assembly and succession of communities, as well as predicting their responses to future climate change across spatial or temporal scales.

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In recent years, the research on the effects of urban road green space on local pollutant dispersion has been quite active, with mounting empirical results. Due to the lack of summary and arrangement, however, it is difficult to effectively guide the practice of urban green space construction. In this review, the road and green belt research objects were classified. Based on two common methods of field measurement and numerical simulation, the pollutant concentration index, vegetation characters and other influencing factors involved in relevant research were systematically reviewed. The effects of road green space on dispersion of local pollution were summarized. Future research should be strengthened in the aspects of expanding research objects, comprehensively considering multiple ecological processes, integrating two basic methods, and improving the accuracy and richness of data.

Contrasting Patterns and Drivers of Soil Fungal Communities between Two Ecosystems Divided by the Treeline.

The treeline is a sensitive region of the terrestrial ecosystem responding to climate change. However, studies on the composition and formation mechanisms of soil fungal communities across the treeline are still lacking. In this study, we investigated the patterns of soil fungal community composition and interactions among functional guilds above and below the treeline using Illumina high-throughput sequencing and ecological network analysis. The results showed that there were significant differences in the soil environment and soil fungal community composition between the two ecosystems above and below the treeline. At the local scale of this study, geographic distance and environmental factors affected the composition of the soil fungal community. Soil temperature was an important environmental predictor of soil fungal community composition. Species in soil fungal communities in the subalpine meadow were more closely related to each other compared to those in the montane forest. Furthermore, the soil fungal community in montane forest was more stable. Our findings contribute to a better understanding of how mountain ecological functions respond to global climate change.

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Identifying the complexity of diversity pattern of various taxa within a community is a challenge for ecologist. Scaling law is one of the suitable ways to detecting the complex ecological structure. In this study, we explored the scaling laws of soil fauna diversity pattern along an altitudinal gradient by multifractal analysis, and compared the difference of multifractal spectra between the litter and the soil layers. Consistent with results from plant communities in previous studies, there was power law scaling law for soil fauna diversity, i.e., richness, the exponential of Shannon's Diversity Index, and the inverse Simpson's Diversity Index. Moreover, power law scaling law also existed for the richness changes of different relative abundance species in both litter and soil layers. Although multifractal characteristics existed for both litter layer and soil layer of soil fauna diversity, the fractal structure of the diversity in the litter layer was more even than that in the soil layer, and the scaling properties of dominant and rare species showed different patterns in multifractal spectra between litter layer and soil layer. In conclusion, there were power law scaling laws for soil fauna diversity which had high richness and abundance along the altitudinal gradient, which would help us uncovering the spatial distribution mechanism of belowground biodiversity.

Impacts of Urbanization Undermine Nestedness of the Plant-Arbuscular Mycorrhizal Fungal Network.

Cities are prone to ecological problems, yet the impacts of rapid global urbanization on the feedback between above- and belowground subsystems remain largely unknown. We sampled the roots of 8 common herbaceous plants within the Fifth Ring (urban areas) and in Jiufeng National Forest Park (rural areas) in Beijing (China) to assess the impacts of urbanization on the network of plant-arbuscular mycorrhizal (AM) fungal associations. Using Illumina MiSeq sequencing, 81 AM fungal OTUs were identified in 78 herb root samples. The Shannon, Simpson, and Pielou indices of root AM fungi in urban areas were significantly higher than those in rural areas. In this study, a significantly nested mycorrhizal association network was observed in rural areas (NODF = 64.68), whereas a non-nested pattern was observed in urban areas (NODF = 55.50). The competition index C-score (0.0769) of AM fungi in urban areas was slightly lower than that in rural areas (0.1431), and the species specialization (d') of 8 host plants and fungal dissimilarity among 8 host plants in urban areas were significantly lower than those in rural areas. Convergent associations among hosts may be an important factor influencing this non-nested pattern of the plant-AM fungi network in urban areas. Generalists, rather than specialists, were enhanced during the establishment of mycorrhizal associations in urban areas. Our results suggest that reduced selectivity of host plants, and generalist promotion and specialist reduction of AM fungi during urbanization may contribute to the non-nested network of plant-AM fungal associations.

A Meta-Analysis on Degraded Alpine Grassland Mediated by Climate Factors: Enlightenment for Ecological Restoration.

Alpine grassland is the main ecosystem on the Qinghai-Tibet Plateau (QTP). Degradation and restoration of alpine grassland are related to ecosystem function and production, livelihood, and wellbeing of local people. Although a large number of studies research degraded alpine grassland, there are debates about degradation patterns of alpine grassland in different areas and widely applicable ecological restoration schemes due to the huge area of the QTP. In this study, we used the meta-analysis method to synthesize 80 individual published studies which were conducted to examine aboveground and underground characteristics in non-degradation (ND), light degradation (LD), moderate degradation (MD), heavy degradation (HD), and extreme degradation (ED) of alpine grassland on the QTP. Results showed that aboveground biomass (AGB), belowground biomass (BGB), Shannon-Wiener index (H'), soil moisture (SM), soil organic carbon (SOC), soil total nitrogen (TN), and available nitrogen (AN) gradually decreased along the degradation gradient, whereas soil bulk density (BD) and soil pH gradually increased. In spite of a tendency to soil desertification, losses of other soil nutrients and reduction of enzymes, there was no linear relationship between the variations with degradation gradient. Moreover, the decreasing extent of TN was smaller in areas with higher precipitation and temperature, and the decreasing extent of AGB, SOC, and TN was larger in areas with a higher extent of corresponding variables in the stage of ND during alpine grassland degradation. These findings suggest that in areas with higher precipitation and temperature, reseeding and sward cleavage can be used for restoration on degraded alpine grassland. Fencing and fertilization can be used for alpine grassland restoration in areas with lower precipitation and temperature. Microbial enzymes should not be used to restore degraded alpine grassland on a large scale on the QTP without detailed investigation and analysis. Future studies should pay more attention to the effects of climate factors on degradation processes and specific ecological restoration strategies in different regions of the QTP.

Wetland loss under the impact of agricultural development in the Sanjiang Plain, NE China.

Wetland loss has been the major environmental problem in Sanjiang Plain, NE China in recent years because of the dramatic agricultural development. We determined the spatial associations of the wetland loss rates in an 11,000-km(2) study area for two intervals of period 1 (1975-1989) and period 2 (1989-2004) spanning 30 years by using geographic information systems. The landscape of this area was simple with five categories, composed of ten types, and including three natural wetland types--open water, marsh, and wet meadow. Extensive agriculture was the principal cultivation form in terms of large size farm units in the area. Agriculture has become the principal land use category replacing natural wetlands over the 30-year period. It has changed the whole landscape of the region and the landscape pattern, causing wetland loss and fragmentation. The wetland loss rate of the area was very different between the two intervals, while wetland loss was not uniform throughout the region and was influenced by the landscape characteristics, such as topography, geomorphology, and the location of the wetlands in the watershed. Despite the remarkable land use changes, the wetlands distribution in the landscapes was similar compared to their original pattern. These results indicated that agricultural development affected the areas more than the distribution pattern of the wetlands in this region.

Cd heavy metal and plants, rather than soil nutrient conditions, affect soil arbuscular mycorrhizal fungal diversity in green spaces during urbanization.

Urbanization accelerates pollution and habitat fragmentation, and the mechanism that shapes the arbuscular mycorrhizal (AM) fungal community in urban ecosystem still remains poorly understood. In this study, soil samples from 23 sites (from rural to urban), belonging to 4 green space types (country park, Co; urban park, Pa; roadside green space1, RoP1; and roadside green space2, RoP2), were collected to assess the effects of the urbanization on the AM fungal diversity. Using 454 pyrosequencing, a total of 79 AM fungal OTUs were uncovered. We found that urbanization showed a neutral effect on Shannon diversity, Simpson diversity, Pielou diversity, and community composition of the AM fungi. Within urban areas, the composition of AM fungal community was significantly different between RoP1 and RoP2. The db-RDA analysis of RoP1 and RoP2 revealed that the soil Cd accounted for the largest community composition variation, with an explanation rate of 20.5%, followed by the SOC (15.1%). Across 23 sites, Cd may have an obvious ecological toxicity on AM fungi, with significantly negative correlations between the soil Cd content and the AM fungal species richness and evenness. The AM fungal community also indicated significantly Mantel correlation with the soil Cd contents. Additionally, high herbaceous richness promoted rich AM fungi. The herbaceous composition, not the richness, has a significant impact on the AM fungal community composition. This study suggests that the toxicity of Cd from traffic should receive more attention during urban green space construction and management, and reasonable plant configuration contributed to the maintenance of the AM fungal community.

Environmental correlates underlying elevational richness, abundance, and biomass patterns of multi-feeding guilds in litter invertebrates across the treeline.

Elevational richness patterns and underlying environmental correlates have contributed greatly to a range of general theories of biodiversity. However, the mechanisms underlying elevational abundance and biomass patterns across several trophic levels in belowground food webs remain largely unknown. In this study, we aimed to disentangle the relationships between the elevational patterns of different trophic levels of litter invertebrates and their underlying environmental correlates for two contrasting ecosystems separated by the treeline. We sampled 119 plots from 1020 to 1770 asl in forest and 21 plots from 1790 to 2280 asl in meadow on Dongling Mountain, northwest of Beijing, China. Four functional guilds were divided based on feeding regime: omnivores, herbivores, predators, and detritivores. We used eigenvector-based spatial filters to account for spatial autocorrelation and multi-model selection to determine the best environmental correlates for the community attributes of the different feeding guilds. The results showed that the richness, abundance and biomass of omnivores declined with increasing elevation in the meadow, whereas there was a hump-shaped richness pattern for detritivores. The richness and abundance of different feeding guilds were positively correlated in the forest, while not in the meadow. In the forest, the variances of richness in omnivores, predators, and detritivores were mostly correlated with litter thickness, with omnivores being best explained by mean annual temperature in the meadow. In conclusion, hump-shaped elevational richness, abundance and biomass patterns driven by the forest gradient below the treeline existed in all feeding guilds of litter invertebrates. Climate replaced productivity as the primary factor that drove the richness patterns of omnivores above the treeline, whereas heterogeneity replaced climate for herbivores. Our results highlight that the correlated elevational richness, abundance, and biomass patterns of feeding guilds are ecosystem-dependent and that the underlying environmental correlates shifted at the treeline for most feeding guilds.