Non-invasive estimation of muscle fibre size from high-density electromyography.

Because of the biophysical relation between muscle fibre diameter and the propagation velocity of action potentials along the muscle fibres, motor unit conduction velocity could be a non-invasive index of muscle fibre size in humans. However, the relation between motor unit conduction velocity and fibre size has been only assessed indirectly in animal models and in human patients with invasive intramuscular EMG recordings, or it has been mathematically derived from computer simulations. By combining advanced non-invasive techniques to record motor unit activity in vivo, i.e. high-density surface EMG, with the gold standard technique for muscle tissue sampling, i.e. muscle biopsy, here we investigated the relation between the conduction velocity of populations of motor units identified from the biceps brachii muscle, and muscle fibre diameter. We demonstrate the possibility of predicting muscle fibre diameter (R2  = 0.66) and cross-sectional area (R2  = 0.65) from conduction velocity estimates with low systematic bias (∼2% and ∼4% respectively) and a relatively low margin of individual error (∼8% and ∼16%, respectively). The proposed neuromuscular interface opens new perspectives in the use of high-density EMG as a non-invasive tool to estimate muscle fibre size without the need of surgical biopsy sampling. The non-invasive nature of high-density surface EMG for the assessment of muscle fibre size may be useful in studies monitoring child development, ageing, space and exercise physiology, although the applicability and validity of the proposed methodology need to be more directly assessed in these specific populations by future studies. KEY POINTS: Because of the biophysical relation between muscle fibre size and the propagation velocity of action potentials along the sarcolemma, motor unit conduction velocity could represent a potential non-invasive candidate for estimating muscle fibre size in vivo. This relation has been previously assessed in animal models and humans with invasive techniques, or it has been mathematically derived from simulations. By combining high-density surface EMG with muscle biopsy, here we explored the relation between the conduction velocity of populations of motor units and muscle fibre size in healthy individuals. Our results confirmed that motor unit conduction velocity can be considered as a novel biomarker of fibre size, which can be adopted to predict muscle fibre diameter and cross-sectional area with low systematic bias and margin of individual error. The proposed neuromuscular interface opens new perspectives in the use of high-density EMG as a non-invasive tool to estimate muscle fibre size without the need of surgical biopsy sampling.

Associations of muscle volume of individual human plantar intrinsic foot muscles with morphological profiles of the foot.

Human plantar intrinsic foot muscles consist of 10 muscles that originate and insert within the sole of the foot. It is known that the anatomical cross-sectional area (ACSA) and muscle thickness of two plantar intrinsic foot muscles, the flexor hallucis brevis (FHB) and abductor hallucis (ABH), associate with morphological parameters of the foot, such as total and truncated foot length and navicular height. However, it is unclear how the size for each of the plantar intrinsic foot muscles associates with various morphological profiles of the foot. This study aimed to elucidate this subject. By using magnetic resonance imaging (MRI), serial images of the right foot were obtained in 13 young adult men without foot deformities. From the obtained MR images, ACSA for each of the individual plantar intrinsic foot muscles was analyzed along the foot length, and then its muscle volume (MV) was calculated. The analyzed muscles were the abductor digiti minimi (ABDM), ABH, adductor hallucis oblique head (ADDH-OH), adductor hallucis transverse head (ADDH-TH), flexor digitorum brevis (FDB), FHB, and quadratus plantae (QP). Furthermore, MV of the whole plantar intrinsic foot muscle (WHOLE) was defined as the total MVs of all the analyzed muscles. As morphological parameters, total foot length, truncated foot length, forefoot width, ball circumference, instep circumference, navicular height, great toe eversion angle, and little toe inversion angle were measured using a laser three-dimensional foot scanner in standing and sitting conditions. In addition, navicular drop (ND) and normalized truncated navicular height (NTNH) were also calculated as medial longitudinal arch (MLA) height indices. The MV of WHOLE was significantly associated with the forefoot width, ball circumference, and instep circumference (r = 0.647-0.711, p = 0.006-0.013). Positive correlations were found between the forefoot width and MV of FHB, FDB, and QP (r = 0.564-0.653, p = 0.015-0.045), between the ball circumference and MV of QP (r = 0.559, p = 0.047), between the instep circumference and MV of FHB (r = 0.609, p = 0.027), and between the little toe inversion angle and MV of QP (r = 0.570, p = 0.042). The MVs of ABH, ABDM, and ADDH-OH were not significantly correlated with any morphological parameters of the foot. Similarly, no significant correlations were found between MV of each muscle and either of the MLA height indices (ND and NTNH). Thus, the current results indicate that forefoot width and circumferential parameters (instep and ball circumference), not MLA height, associate with the size of the whole plantar intrinsic foot muscles, especially those specialized in toe flexion (FHB, FDB, and QP).

Corticospinal excitability and motor representation after long-term resistance training.

It is poorly understood how the central nervous system adapts to resistance training, especially after years of exposure. We compared corticospinal excitability and motor representation assessed with transcranial magnetic stimulation (TMS) between long-term resistance trained (LRT, ≥3 years) versus untrained (UNT) males (n = 15/group). Motor-evoked potentials (MEPs) were obtained from the biceps brachii during isometric elbow flexion. Stimulus-response curves were created at the hotspot during 10% maximum voluntary torque (MVT) contractions. Maximum peak-to-peak MEP amplitude (MEPmax) was acquired with 100% stimulator output intensity, whilst 25%-100% MVT was produced. Maps were created during 10% MVT contractions, with an individualised TMS intensity eliciting 20% MEPmax at the hotspot. LRT had a 48% lower stimulus-response curve slope than UNT (p < .05). LRT also had a 66% larger absolute map size, although TMS intensity used for mapping was greater in LRT versus UNT (48% vs. 26% above active motor threshold) to achieve a target 20% MEPmax at the hotspot, due to the lower slope of LRT. Map size was strongly correlated with the TMS intensity used for mapping (r = 0.776, p < .001). Once map size was normalised to TMS intensity, there was no difference between the groups (p = .683). We conclude that LRT had a lower stimulus-response curve slope/excitability, suggesting higher neural efficiency. TMS map size was overwhelmingly determined by TMS intensity, even when the MEP response at the hotspot was matched among individuals, likely due to larger current spread with higher intensities. Motor representation appears similar between LRT and UNT given no difference in the normalised map size.

Relationship between Trunk Muscularity and Club Head Speed in Male Golfers.

This study examined how the volume of trunk muscles and its bilateral asymmetry are related to club head speed in golfers. Fourteen right-handed male golfers performed five driver shots, and the club head speed for each trial was calculated from a three-dimensional reflective marker position of the club head immediately before impact. The volume of each side of the rectus abdominis, erector spinae, psoas major, quadratus lumborum, lateral abdominal wall muscle, and multifidus was determined using magnetic resonance imaging. For each muscle, the ratio of the larger to smaller side in muscle volume was calculated to assess bilateral asymmetry. The club head speed correlated positively with the volume of each side of the rectus abdominis and erector spinae, left quadratus lumborum, and the asymmetric ratio of the psoas major (r=0.595-0.747), but negatively with the asymmetric ratio of the quadratus lumborum (r=-0.641). Multiple regression analysis revealed that the right erector spinae volume and the asymmetric ratio of the psoas major were significant contributors for the club head speed (R2=0.797). These results indicate that the variation in the club head speed can be strongly explained by the absolute volume and bilateral asymmetry of specific trunk muscles.

Suspended Push-up Training Augments Size of not only Upper Limb but also Abdominal Muscles.

We investigated the effects of sling-based, suspended push-up training on muscle size and function of upper limb and abdominal muscles. Eight men conducted suspended push-ups to failure 3 sets/session, 3 sessions/week, for 8 weeks. The maximum number of push-ups during training gradually and significantly increased from the first to last training session (+92%), suggesting improved muscle endurance. After the training, muscle thickness of the elbow extensors (+16%) and flexors (+3%), as well as abdominal muscles (rectus abdominis: RA,+27%; external oblique: EO,+14%) significantly increased. No changes occurred in maximum isometric strength of elbow extension or flexion, nor in 1-repetition maximum bench press. In a follow-up experiment, electromyograms (EMGs) of RA, EO and internal oblique (IO) during suspended push-ups to failure were measured and normalized to those during maximum voluntary contraction of each muscle (% EMGmvc) in six men. EMG significantly increased when reaching failure in all muscles (RA: 46-88%, EO: 32-50%, IO: 19-52%, start-end), and was particularly high in RA. These results suggest that suspended push-up training can augment size of not only upper limb but also abdominal muscles, likely attributable to high muscle activities during exercise; however, this does not necessarily improve maximum strength after training thus warrants careful interpretation/application.

Effect of abdominal bracing training on strength and power of trunk and lower limb muscles.

PURPOSE: It is unknown whether maximal voluntary co-contraction of abdominal muscles, called abdominal bracing, can be a training maneuver for improving strength and power of trunk and lower limb muscles. The present study aimed to elucidate this. METHODS: Twenty young adult men (23.3 ± 1.8 years) were allocated to training (TG, n = 11) or control (CG, n = 9) group. TG conducted an 8-week training program (3 days/week) consisting of 2-s maximal abdominal bracing followed by 2-s muscle relaxation (5 × 10 repetitions/day). Maximal voluntary isometric strength during trunk flexion and extension, hip extension, and knee extension, maximal lifting power from sitting position, and the thicknesses of abdominal muscles were measured before and after the intervention. In addition, surface electromyograms from trunk and lower limb muscles and intra-abdominal pressure (IAP) during the maximal abdominal bracing and maximal lifting tasks were also determined. RESULTS: After the intervention, TG showed significant increases in isometric trunk extension (+14.4 %) and hip extension (+34.7 %) strength and maximal lifting power (+15.6 %), while CG did not show any changes in strength and power variables. Furthermore, TG had significant gains in the muscle thickness of the oblique internal (+22.4 %), maximal IAP during abdominal bracing (+36.8 %), and the rate of IAP rise during lifting task (+58.8 %), without corresponding changes in CG. CONCLUSION: The current study indicates that a training style with maximal voluntary co-contraction of abdominal muscles can be an effective maneuver for increasing strength and power during movements involving trunk and hip extensions, without using external load.

Downhill walking training with and without exercise-induced muscle damage similarly increase knee extensor strength.

This study examined whether avoiding or experiencing exercise-induced muscle damage (EIMD) influences strength gain after downhill walking training. Healthy young males performed treadmill downhill walking (gradient: -28%, velocity: 5 km · h(-1) and load: 10% of body mass) 1 session per week for four weeks using either a ramp-up protocol (n = 16), where exercise duration was gradually increased from 10 to 30, 50 and 70 min over four sessions, or a constant protocol (n = 14), where exercise duration was 40 min for all four sessions. Indirect markers of EIMD were measured throughout the training period. Maximal knee extension torque in eccentric (-1.05 rad·s(-1)), isometric and concentric (1.05 rad·s(-1)) conditions were measured at pre- and post-training. The ramp-up group showed no indications of EIMD throughout the training period (e.g., plasma creatine kinase (CK) activity: always <185 U · L(-1)) while EIMD was evident after the first session in the constant group (CK: peak 485 U · L(-1)). Both groups significantly increased maximal knee extension torque in all conditions with greater gains in eccentric (ramp-up: +19%, constant: +21%) than isometric (+16%, +15%) and concentric (+12%, +10%) strength without any significant group-difference. The current results suggest that EIMD can be avoided by the ramp-up protocol and is not a major determinant of training-induced strength gain.

Neuromuscular adaptations following 12-week maximal voluntary co-contraction training.

PURPOSE: The present study examined neuromuscular adaptations following 12-week maximal voluntary co-contraction training. METHODS: Sixteen young men were allocated to training (TG, n = 9) or control (CG, n = 7) group. TG conducted a training program (3 days/week), which consisted of 4-s maximal voluntary contractions of elbow flexors and extensors by simultaneously contracting both muscle groups at 90° of the elbow joint, followed by 4-s muscle relaxation (10 repetitions/set, 5 sets/day) for 12 weeks. In addition to the muscle thicknesses of elbow flexors and extensors, the torque and electromyograms (EMGs) of the two muscle groups during isometric maximal voluntary contraction (MVC) were determined before (Pre), after 4 weeks, and 12 weeks of intervention. RESULTS: After intervention, CG showed no significant changes in all measured variables. In TG, MVC torque significantly increased in both elbow flexors (+13 % at 4 weeks and +15 % at 12 weeks) and extensors (+27 % at 4 weeks and +46 % at 12 weeks) from Pre. Muscle thickness also significantly increased in both elbow flexors (+4 %) and extensors (+4 %) at 12 weeks. Agonist EMG activities during MVC significantly increased in both elbow flexors (+31 % at 4 weeks and +44 % at 12 weeks) and extensors (+27 % at 4 weeks and +40 % at 12 weeks), without changes in antagonist involuntary coactivation level in both muscle groups. CONCLUSION: These results indicate that maximal voluntary co-contraction is applicable as a training modality for increasing the size and strength of antagonistic muscle pairs without increasing involuntary coactivation level.

Plantar intrinsic foot muscle activity and its relationship with postural sway during tiptoe standing in ballet dancers and non-dancers.

BACKGROUND: Minimizing postural sway during tiptoe standing is essential for ballet dancers. Investigation of the activity of the plantar intrinsic foot muscles (PIFMs) may provide insight into postural sway in dancers. Herein, we compared PIFM activity during tiptoe standing between dancers and non-dancers and examined its relationship with postural sway. METHODS: We enrolled 14 female ballet dancers and 13 female non-dancers. Electromyography (EMG) amplitudes of 64 channels of PIFMs and center of pressure (COP) data were recorded during bipedal tiptoe standing tasks performed with ankle plantarflexion angles of 20°, 40°, and 60° (dancers only). The EMG amplitudes were normalized to those during the maximum voluntary contraction, and the muscle activity level and its coefficient of variation over time (EMG-CVtime) during the task were assessed. Standard deviations in the anteroposterior and mediolateral directions, velocity, and area were calculated from the COP data. RESULTS: Most COP and EMG variables were significantly lower in dancers than in non-dancers in both the 20° and 40° tasks (p < 0.05). Significant correlations were found between most combinations of the COP and EMG variables in both the 20° and 40° tasks in the whole cohort (r = 0.468-0.807, p ≤ 0.014). In the 60° task in dancers, COP velocity was strongly correlated with EMG-CVtime (r = 0.700, p = 0.005). CONCLUSION: These results provide novel evidence that the PIFMs do not require high activity, but rather that its low, steady activity is the key, to achieve less postural sway during bipedal tiptoe standing in dancers.

Differences in the Size of Individual Plantar Intrinsic Foot Muscles Between Ballet Dancers and Non-Dancers.

INTRODUCTION: In classic ballet, choreography often involves tiptoe standing. Tiptoe standing requires a high and stable foot arch structure, which is achieved by contraction of the plantar intrinsic foot muscles (PIFMs). Long-term repetitive loading with a specific movement can induce hypertrophic adaptation of the associated muscles. For dancers, however, limited information on the size of individual PIFMs is available from previous studies. The purpose of this study was to determine the differences in the sizes of 10 individual PIFMs between dancers and non-dancers. METHODS: Muscle volumes (MVs) of 10 individual PIFMs were measured using magnetic resonance imaging in 15 female dancers and 15 female non-dancers. Muscles analyzed included abductor hallucis, flexor digitorum brevis, abductor digiti minimi, quadratus plantae, lumbricals, flexor hallucis brevis, adductor hallucis oblique head, adductor hallucis transverse head, flexor digiti minimi, plantar/dorsal interossei. In addition to absolute MVs, relative MVs normalized to body mass (rMVBM) and the percentage of individual MVs relative to the sum of 10 individual PIFM MVs (%MVWHOLE) were calculated. RESULTS: The absolute MVs of 6 individual PIFMs, including the flexor digitorum brevis and lumbricals, were +16% to 59% larger in dancers than in non-dancers (P ≤ .048). The rMVBM of all individual PIFMs were +35% to 95% larger in dancers than in non-dancers (P ≤ .019). The %MVWHOLE of the flexor digitorum brevis and lumbricals were +10% to 36% higher (P ≤ .014) and those of the abductor digiti minimi and adductor hallucis oblique head were +8% to 11% lower (P ≤ .037) in dancers than in non-dancers. CONCLUSIONS: For all 3 MV measures, only the flexor digitorum brevis and lumbricals, which are functionally specialized for flexion of the second to fifth metatarsophalangeal joints, were consistently larger in dancers than in non-dancers. This may be due to long-term repetitive loading on these PIFMs during ballet training involving tiptoe standing.

Sex differences in muscle morphology between male and female sprinters.

There is a marked difference between males and females in sprint running performance, yet a comprehensive investigation of sex differences in the muscle morphology of sprinters, which could explain the performance differences, remains to be completed. This study compared muscle volumes of 23 individual leg muscles and 5 functional muscle groups, assessed with 3 T magnetic resonance imaging, between male (n = 31) and female (n = 22) sprinters, as well as subgroups of elite males (EM, n = 5), elite females (EF, n = 5), and performance-matched (to elite females) males (PMMEF, n = 6). Differences in muscle volume distribution between EM, EF, and unathletic male (UM) controls were also assessed. For the full cohorts, male sprinters were more muscular than their female counterparts, but the differences were nonuniform and anatomically variable, with the largest differences in the hip extensors and flexors. However, among elite sprinters the sex differences in the volume of the functional muscle groups were almost uniform (absolute volume +47-53%), and the muscle volume distribution of EM was more similar to EF than to UM (P < 0.039). For PMMEF, relative hip extensor volume, but not stature or percent body fat, differentiated for performance (PMMEF and EF < EM) rather than sex. In conclusion, although the full cohorts of sprinters showed a marked sex difference in the amount and distribution of muscle mass, elite sprinters appeared to be selected for a common muscle distribution phenotype that for these elite subgroups was a stronger effect than that of sex. Relative hip extensor muscle volume, rather than stature, percent body fat, or total relative muscle volume, appeared to be the primary determinant of the sex difference in performance.NEW & NOTEWORTHY We present novel evidence suggesting muscle volume, specifically relative hip extensor volume, may be a primary deterministic variable for the sex difference in sprint performance, such that with matched sprint times, male and female sprinters may be expected to have equivalent muscle morphology. We highlight striking similarities in distribution of leg muscle mass between elite male and female sprinters and provide evidence for the existence of a muscular distribution phenotype specific to elite sprinters, irrespective of sex.

Long-Term Resistance Trained Human Muscles Have More Fibres, More Myofibrils and Tighter Myofilament Packing than Untrained.

INTRODUCTION: Increases in skeletal muscle size occur in response to prolonged exposure to resistance training that is typically ascribed to increased muscle fibre size. Whether muscle fibre number also changes remains controversial, and a paucity of data exists about myofibrillar structure. This cross-sectional study compared muscle fibre and myofibril characteristics in long-term resistance-trained (LRT) versus untrained (UNT) individuals. METHODS: The maximal anatomical cross-sectional area (ACSAmax) of the biceps brachii muscle was measured by MRI in 16 LRT (5.9 ± 3.5 years' experience) and 13 UNT males. A muscle biopsy was taken from the biceps brachii to measure muscle fibre area, myofibril area and myosin spacing. Muscle fibre number, myofibril number in total and per fibre were estimated by dividing ACSAmax by muscle fibre area or myofibril area, and muscle fibre area by myofibril area, respectively. RESULTS: Compared to UNT, LRT individuals had greater ACSAmax (+70%, P < 0.001), fibre area (+29%, P = 0.028), fibre number (+34%, P = 0.013), and myofibril number per fibre (+49%, P = 0.034) and in total (+105%, P < 0.001). LRT individuals also had smaller myosin spacing (-7%, P = 0.004; i.e. greater packing density) and a tendency towards smaller myofibril area (-16%, P = 0.074). ACSAmax was positively correlated with fibre area ( r = 0.526), fibre number ( r = 0.445) and myofibril number (in total r = 0.873 and per fibre r = 0.566), and negatively correlated with myofibril area ( r = -0.456) and myosin spacing ( r = -0.382) (all P < 0.05). CONCLUSIONS: The larger muscles of LRT individuals exhibited more fibres in cross-section and larger muscle fibres, which contained substantially more total myofibrils and more packed myofilaments than UNT participants, suggesting plasticity of muscle ultrastructure.

Hamstrings Hypertrophy is Specific to the Training Exercise: Nordic Hamstring versus Lengthened State Eccentric Training.

INTRODUCTION: The hamstring muscles play a crucial role in sprint running, but are also highly susceptible to strain injuries, particularly within the biceps femoris long head (BFlh). This study compared the adaptations in muscle size and strength of the knee flexors, as well as BFlh muscle and aponeurosis size, after two eccentrically focused knee flexion training regimes: Nordic hamstring training (NHT) or lengthened state eccentric training (LSET, isoinertial weight-stack resistance in an accentuated hip-flexed position), to habitual activity (no training controls: CON). METHODS: 42 healthy young males completed 34 sessions of NHT or LSET over 12 weeks or served as CON (n = 14/group). MRI-measured muscle volume of seven individual knee flexors and BFlh aponeurosis area, and maximum knee flexion torque during eccentric, concentric and isometric contractions were assessed pre- and post-training. RESULTS: LSET induced greater increases in hamstrings (+18% vs +11%) and BFlh (+19% vs +5%) muscle volumes and BFlh aponeurosis area (+9% vs +3%) than NHT (all P ≤ 0.001), with no changes after CON. There were distinctly different patterns of hypertrophy between the two training regimes, largely due to the functional role of the muscles; LSET was more effective for increasing the size of knee flexors that also extend the hip (2.2-fold vs NHT), whereas NHT increased the size of knee flexors that do not extend the hip (1.9-fold vs LSET; both P ≤ 0.001). Changes in maximum eccentric torque differed only between LSET and CON (+17% vs +4%; P = 0.009), with NHT (+11%) in-between. CONCLUSIONS: These results suggest that LSET is superior to NHT in inducing overall hamstrings and BFlh hypertrophy, potentially contributing to better sprint performance improvements and protection against hamstring strain injuries than NHT.

Trunk muscle activities during abdominal bracing: comparison among muscles and exercises.

Abdominal bracing is often adopted in fitness and sports conditioning programs. However, there is little information on how muscular activities during the task differ among the muscle groups located in the trunk and from those during other trunk exercises. The present study aimed to quantify muscular activity levels during abdominal bracing with respect to muscle- and exercise-related differences. Ten healthy young adult men performed five static (abdominal bracing, abdominal hollowing, prone, side, and supine plank) and five dynamic (V- sits, curl-ups, sit-ups, and back extensions on the floor and on a bench) exercises. Surface electromyogram (EMG) activities of the rectus abdominis (RA), external oblique (EO), internal oblique (IO), and erector spinae (ES) muscles were recorded in each of the exercises. The EMG data were normalized to those obtained during maximal voluntary contraction of each muscle (% EMGmax). The % EMGmax value during abdominal bracing was significantly higher in IO (60%) than in the other muscles (RA: 18%, EO: 27%, ES: 19%). The % EMGmax values for RA, EO, and ES were significantly lower in the abdominal bracing than in some of the other exercises such as V-sits and sit-ups for RA and EO and back extensions for ES muscle. However, the % EMGmax value for IO during the abdominal bracing was significantly higher than those in most of the other exercises including dynamic ones such as curl-ups and sit-ups. These results suggest that abdominal bracing is one of the most effective techniques for inducing a higher activation in deep abdominal muscles, such as IO muscle, even compared to dynamic exercises involving trunk flexion/extension movements. Key PointsTrunk muscle activities during abdominal bracing was examined with regard to muscle- and exercise-related differences.Abdominal bracing preferentially activates internal oblique muscles even compared to dynamic exercises involving trunk flexion/extension movements.Abdominal bracing should be included in exercise programs when the goal is to improve spine stability.

A classification of the plantar intrinsic foot muscles based on the physiological cross-sectional area and muscle fiber length in healthy young adult males.

BACKGROUND: Plantar intrinsic foot muscles (PIFMs) are composed of 10 muscles and play an essential role in achieving functional diversity in the foot. Previous studies have identified that the morphological profiles of PIFMs vary between individuals. The morphological profiles of a muscle theoretically reflect its output potentials: the physiological cross-sectional area (PCSA) of a muscle is proportional to its maximum force generation, and the muscle fiber length (FL) is its shortening velocity. This implies that the PCSA and FL may be useful variables for characterizing the functional diversity of the individual PIFM. The purpose of this study was to examine how individual PIFMs can be classified based on their PCSA and FL. METHODS: In 26 healthy young adult males, the muscle volume and muscle length of seven PIFMs (abductor hallucis, ABDH; abductor digiti minimi, ABDM; adductor hallucis oblique head, ADDH-OH; ADDH transverse head, ADDH-TH; flexor digitorum brevis, FDB; flexor hallucis brevis, FHB; quadratus plantae, QP) were measured using magnetic resonance imaging. The PCSA and FL of each of the seven PIFMs were then estimated by combining the data measured from the participants and those of muscle architectural parameters documented from cadavers in previous studies. A total of 182 data samples (26 participants × 7 muscles) were classified into clusters using k-means cluster analysis. The optimal number of clusters was evaluated using the elbow method. RESULTS: The data samples of PIFMs were assigned to four clusters with different morphological profiles: ADDH-OH and FHB, characterised by large PCSA and short FL (high force generation and slow shortening velocity potentials); ABDM and FDB, moderate PCSA and moderate FL (moderate force generation and moderate shortening velocity potentials); QP, moderate PCSA and long FL (moderate force generation and rapid shortening velocity potentials); ADDH-TH, small PCSA and moderate FL (low force generation and moderate shortening velocity potentials). ABDH components were assigned equivalently to the first and second clusters. CONCLUSIONS: The approach adopted in this study may provide a novel perspective for interpreting the PIFMs' function based on their maximal force generation and shortening velocity potentials.

Triceps brachii hypertrophy is substantially greater after elbow extension training performed in the overhead versus neutral arm position.

The biarticular triceps brachii long head (TBLong) is lengthened more in the overhead than neutral arm position. We compared triceps brachii hypertrophy after elbow extension training performed in the overhead vs. neutral arm position. Using a cable machine, 21 adults conducted elbow extensions (90-0°) with one arm in the overhead (Overhead-Arm) and the other arm in the neutral (Neutral-Arm) position at 70% one-repetition maximum (1RM), 10 reps/set, 5 sets/session, 2 sessions/week for 12 weeks. Training load was gradually increased (+5% 1RM/session) when the preceding session was completed without repetition failure. 1RM of the assigned condition and MRI-measured muscle volume of the TBLong, monoarticular lateral and medial heads (TBLat+Med), and whole triceps brachii (Whole-TB) were assessed pre- and post-training. Training load and 1RM increased in both arms similarly (+62-71% at post, P = 0.285), while their absolute values/weights were always lower in Overhead-Arm (-34-39%, P < 0.001). Changes in muscle volume in Overhead-Arm compared to Neutral-Arm were 1.5-fold greater for the TBLong (+28.5% vs. +19.6%, Cohen's d = 0.61, P < 0.001), 1.4-fold greater for the TBLat+Med (+14.6% vs. +10.5%, d = 0.39, P = 0.002), and 1.4-fold greater for the Whole-TB (+19.9% vs. +13.9%, d = 0.54, P < 0.001). In conclusion, triceps brachii hypertrophy was substantially greater after elbow extension training performed in the overhead versus neutral arm position, even with lower absolute loads used during the training.HighlightsGrowing evidence suggests that resistance training at long muscle lengths promotes muscle hypertrophy, but its practical applications are yet to be explored.Triceps brachii muscle hypertrophy was substantially greater after cable elbow extension training performed in the overhead than neutral arm position, particularly in the biarticular triceps brachii long head, even with lower absolute loads lifted (i.e. lower mechanical stress to muscles/joints).Cable elbow extension training should be performed in the overhead rather than neutral arm position if one aims to maximise muscle hypertrophy of the triceps brachii or to prevent atrophy of this muscle.

Triceps surae muscle hypertrophy is greater after standing versus seated calf-raise training.

Background: The triceps surae muscle plays important roles in fundamental human movements. However, this muscle is relatively unresponsive to resistance training (difficult to hypertrophy) but prone to atrophy with inactivity compared with other muscles. Thus, identifying an effective training modality for the triceps surae is warranted. This study compared triceps surae muscle hypertrophy after standing/knee-extended versus seated/knee-flexed plantarflexion (calf-raise) training, where the gastrocnemius is lengthened and shortened, respectively. Methods: Fourteen untrained adults conducted calf-raise training with one leg in a standing/knee-extended position and the other leg in a seated/knee 90°-flexed position at 70% of one-repetition maximum. Each leg performed 10 repetitions/set, 5 sets/session, 2 sessions/week for 12 weeks. Before and after the intervention, magnetic resonance imaging scans were obtained to assess muscle volume of each and the whole triceps surae. Results: Muscle volume significantly increased in all three muscles and the whole triceps surae for both legs (p ≤ 0.031), except for the gastrocnemius muscles of the seated condition leg (p = 0.147-0.508). The changes in muscle volume were significantly greater for the standing than seated condition leg in the lateral gastrocnemius (12.4% vs. 1.7%), medial gastrocnemius (9.2% vs. 0.6%), and whole triceps surae (5.6% vs. 2.1%) (p ≤ 0.011), but similar between legs in the soleus (2.1% vs. 2.9%, p = 0.410). Conclusion: Standing calf-raise was by far more effective, therefore recommended, than seated calf-raise for inducing muscle hypertrophy of the gastrocnemius and consequently the whole triceps surae. This result and similar between-condition hypertrophy in the soleus collectively suggest that training at long muscle lengths promotes muscle hypertrophy.

Heavy Resistance Training Versus Plyometric Training for Improving Running Economy and Running Time Trial Performance: A Systematic Review and Meta-analysis.

BACKGROUND: As an adjunct to running training, heavy resistance and plyometric training have recently drawn attention as potential training modalities that improve running economy and running time trial performance. However, the comparative effectiveness is unknown. The present systematic review and meta-analysis aimed to determine if there are different effects of heavy resistance training versus plyometric training as an adjunct to running training on running economy and running time trial performance in long-distance runners. METHODS: Electronic databases of PubMed, Web of Science, and SPORTDiscus were searched. Twenty-two studies completely satisfied the selection criteria. Data on running economy and running time trial performance were extracted for the meta-analysis. Subgroup analyses were performed with selected potential moderators. RESULTS: The pooled effect size for running economy in heavy resistance training was greater (g = - 0.32 [95% confidence intervals [CIs] - 0.55 to - 0.10]: effect size = small) than that in plyometric training (g = -0.13 [95% CIs - 0.47 to 0.21]: trivial). The effect on running time trial performance was also larger in heavy resistance training (g = - 0.24 [95% CIs - 1.04 to - 0.55]: small) than that in plyometric training (g = - 0.17 [95% CIs - 0.27 to - 0.06]: trivial). Heavy resistance training with nearly maximal loads (≥ 90% of 1 repetition maximum [1RM], g = - 0.31 [95% CIs - 0.61 to - 0.02]: small) provided greater effects than those with lower loads (< 90% 1RM, g = - 0.17 [95% CIs - 1.05 to 0.70]: trivial). Greater effects were evident when training was performed for a longer period in both heavy resistance (10-14 weeks, g = - 0.45 [95% CIs - 0.83 to - 0.08]: small vs. 6-8 weeks, g = - 0.21 [95% CIs - 0.56 to 0.15]: small) and plyometric training (8-10 weeks, g = 0.26 [95% CIs - 0.67 to 0.15]: small vs. 4-6 weeks, g = - 0.06 [95% CIs 0.67 to 0.55]: trivial). CONCLUSIONS: Heavy resistance training, especially with nearly maximal loads, may be superior to plyometric training in improving running economy and running time trial performance. In addition, running economy appears to be improved better when training is performed for a longer period in both heavy resistance and plyometric training.

Associations between the size of individual plantar intrinsic and extrinsic foot muscles and toe flexor strength.

BACKGROUND: The size of the plantar intrinsic and extrinsic foot muscles has been shown to be associated with toe flexor strength (TFS). Previous studies adopted the size of limited plantar intrinsic foot muscles or a compartment containing several muscles as an independent variable for TFS. Among the plantar intrinsic and extrinsic foot muscles, therefore, it is unclear which muscle(s) primarily contributes to TFS production. The present study aimed to clarify this subject. METHODS: In 17 young adult men, a series of anatomical cross-sectional area of individual plantar intrinsic and extrinsic foot muscles was obtained along the foot length and the lower leg length, respectively, using magnetic resonance imaging. Maximal anatomical cross-sectional area (ACSAmax) and muscle volume (MV) for each constituent muscle of the plantar intrinsic foot muscles (flexor hallucis brevis; flexor digitorum brevis, FDB; abductor hallucis; adductor hallucis oblique head, ADDH-OH; adductor hallucis transverse head, ADDH-TH; abductor digiti minimi; quadratus plantae) and extrinsic foot muscles (flexor hallucis longus; flexor digitorum longus) were measured. TFS was measured with a toe grip dynamometry. RESULTS: TFS was significantly associated with the ACSAmax for each of the ADDH-OH (r = 0.674, p = 0.003), ADDH-TH (r = 0.523, p = 0.031), and FDB (r = 0.492, p = 0.045), and the MV of the ADDH-OH (r = 0.582, p = 0.014). As for the ADDH-OH, the correlation coefficient with TFS was not statistically different between ACSAmax and MV (p = 0.189). Stepwise multiple linear regression analysis indicated that ACSAmax and MV of the ADDH-OH alone explained 42 and 29%, respectively, of the variance in TFS. CONCLUSION: The ADDH-OH is the primary contributor to TFS production among the plantar intrinsic and extrinsic foot muscles as the result of the stepwise multiple linear regression analysis.

The Muscle Morphology of Elite Female Sprint Running.

INTRODUCTION: A paucity of research exists examining the importance of muscle morphological and functional characteristics for elite female sprint performance. PURPOSE: This study aimed to compare lower body muscle volumes and vertical jumping power between elite and subelite female sprinters and assess the relationships of these characteristics with sprint race and acceleration performance. METHODS: Five elite (100 m seasons best [SBE 100 ], 11.16 ± 0.06 s) and 17 subelite (SBE 100 , 11.84 ± 0.42 s) female sprinters underwent: 3T magnetic resonance imaging to determine the volume of 23 individual leg muscles/compartments and five functional muscle groups; countermovement jump and 30 m acceleration tests. RESULTS: Total absolute lower body muscle volume was higher in elite versus subelite sprinters (+15%). Elite females exhibited greater muscle volume of the hip flexors (absolute, +28%; relative [to body mass], +19%), hip extensors (absolute, +22%; relative, +14%), and knee extensors (absolute, +21%), demonstrating pronounced anatomically specific muscularity, with relative hip flexor volume alone explaining 48% of sprint performance variability. The relative volume of five individual muscles (sartorius, gluteus maximus, adductor magnus, vastus lateralis, illiopsoas) were both distinct between groups (elite > subelite) and related to SBE 100 ( r = 0.553-0.639), with the combination of the sartorius (41%) and the adductor magnus (17%) explaining 58% of the variance in SBE 100 . Elite female sprinters demonstrated greater absolute countermovement jump power versus subelite, and absolute and relative power were related to both SBE 100 ( r = -0.520 to -0.741) and acceleration performance ( r = 0.569 to 0.808). CONCLUSIONS: This investigation illustrates the distinctive, anatomically specific muscle volume distribution that facilitates elite sprint running in females, and emphasizes the importance of hip flexor and extensor relative muscle volume.