Nitrogen availability prevents oxidative effects of salinity on wheat growth and photosynthesis by up-regulating the antioxidants and osmolytes metabolism, and secondary metabolite accumulation.

BACKGROUND: Salinity is one of the damaging abiotic stress factor. Proper management techniques have been proposed to considerably lower the intensity of salinity on crop growth and productivity. Therefore experiments were conducted to assess the role of improved nitrogen (N) supplementation on the growth and salinity stress tolerance in wheat by analyzing the antioxidants, osmolytes and secondary metabolites. RESULTS: Salinity (100 mM NaCl) stress imparted deleterious effects on the chlorophyll and carotenoid synthesis as well as the photosynthetic efficiency. N supplementation resulted in increased photosynthetic rate, stomatal conductance and internal CO2 concentration with effects being much obvious in seedlings treated with higher N dose. Under non-saline conditions at both N levels, protease and lipoxygenase activity reduced significantly reflecting in reduced oxidative damage. Such effects were accompanied by reduced generation of toxic radicals like hydrogen peroxide and superoxide, and lipid peroxidation in N supplemented seedlings. Antioxidant defence system was up-regulated under saline and non-saline growth conditions due to N supplementation leading to protection of major cellular processes like photosynthesis, membrane structure and function, and mineral assimilation. Increased osmolyte and secondary metabolite accumulation, and redox components in N supplemented plants regulated the ROS metabolism and NaCl tolerance by further strengthening the antioxidant mechanisms. CONCLUSIONS: Findings of present study suggest that N availability regulated the salinity tolerance by reducing Na uptake and strengthening the key tolerance mechanisms.

Spermine application alleviates salinity induced growth and photosynthetic inhibition in Solanum lycopersicum by modulating osmolyte and secondary metabolite accumulation and differentially regulating antioxidant metabolism.

Influence of exogenously applied spermine (Spm) on growth and salinity stress tolerance in tomato was investigated. Salinity reduced growth, chlorophyll synthesis and mineral uptake leading to significant reduction in photosynthesis, however Spm application proved beneficial in alleviating the decline to considerable extent. Applied Spm improved nitrate reductase activity, δ-amino levulinic acid content and gas exchange parameters more apparently at 100 µM than 50 µM concentrations. Spm application enhanced the accumulation of compatible osmolytes including proline, glycine betaine and sugars leading to greater tissue water content and photosynthesis. Salinity stress induced oxidative effects were mitigated by Spm treatment reflected interms of reduced accumulation of reactive oxygen species and the activities of protease and lipoxygenase, hence leading to membrane strengthening and protection of their function. Differential influence of exogenous Spm was evident on the functioning of antioxidant system with SOD, GR and APX activities much higher in Spm treated seedlings than CAT and DHAR. Increased synthesis of GSH, AsA and tocopherol in Spm treated seedlings was obvious thereby helping in maintaining the redox homeostasis and the enzymatic antioxidant functioning. Interestingly Spm application maintained the nitric oxide levels higher than control under normal condition while as lowered its concentrations in salinity stressed seedlings depicting existence of probable interaction. Activities of polyamine metabolizing enzymes was up-regulated and the accumulation of secondary metabolites including phenols and flavonoids also increased due to Spm application. Further studies are required to understand the mechanisms clearly.