RESUMO
Acyl-CoA-binding proteins (ACBPs) are involved in binding and trafficking acyl-CoA esters in eukaryotic cells. ACBPs contain a well-conserved acyl-CoA-binding domain. Their various functions have been characterized in the model plant Arabidopsis and, to a lesser extent, in rice. In this study, genome-wide detection and expression analysis of ACBPs were performed on Elaeis guineensis (oil palm), the most important oil crop in the world. Seven E. guineensis ACBPs were identified and classified into four groups according to their deduced amino acid domain organization. Phylogenetic analysis showed conservation of this family with other higher plants. All seven EgACBPs were expressed in most tissues while their differential expression suggests various functions in specific tissues. For example, EgACBP3 had high expression in inflorescences and stalks while EgACBP1 showed strong expression in leaves. Because of the importance of E. guineensis as an oil crop, expression of EgACBPs was specifically examined during fruit development. EgACBP3 showed high expression throughout mesocarp development, while EgACBP1 had enhanced expression during rapid oil synthesis. In endosperm, both EgACBP1 and EgACBP3 exhibited increased expression during seed development. These results provide important information for further investigations on the biological functions of EgACBPs in various tissues and, in particular, their roles in oil synthesis.
Assuntos
Inibidor da Ligação a Diazepam/genética , Regulação da Expressão Gênica de Plantas , Óleo de Palmeira/metabolismo , Proteínas de Plantas/genética , Sequência de Aminoácidos , Arecaceae/genética , Arecaceae/metabolismo , Inibidor da Ligação a Diazepam/metabolismo , Endosperma/metabolismo , Filogenia , Folhas de Planta/metabolismo , Proteínas de Plantas/metabolismo , Sementes/metabolismo , TranscriptomaRESUMO
We have characterized an oil palm (Elaeis guineensis Jacq.) constitutive promoter that is derived from a translationally control tumor protein (TCTP) gene. The TCTP promoter was fused transcriptionally with the gusA reporter gene and transferred to monocot and dicot systems in order to study its regulatory role in a transient expression study. It was found that the 5' region of TCTP was capable of driving the gusA expression in all the oil palm tissues tested, including immature embryo, embryogenic callus, embryoid, young leaflet from mature palm, green leaf, mesocarp and stem. It could also be used in dicot systems as it was also capable of driving gusA expression in tobacco leaves. The results indicate that the TCTP promoter could be used for the production of recombinant proteins that require constitutive expression in the plant system.
Assuntos
Arecaceae/genética , Regulação da Expressão Gênica de Plantas/genética , Genes de Plantas/genética , Proteínas de Plantas/genética , Regiões Promotoras Genéticas/genética , Sequência de Aminoácidos , Arecaceae/citologia , Arecaceae/fisiologia , Sequência de Bases , Biolística , Northern Blotting , Clonagem Molecular , DNA de Plantas/química , DNA de Plantas/genética , Flores/genética , Flores/fisiologia , Genes Reporter , Dados de Sequência Molecular , Folhas de Planta/genética , Folhas de Planta/fisiologia , Raízes de Plantas/genética , Raízes de Plantas/fisiologia , Plantas Geneticamente Modificadas , Plasmídeos/genética , Reação em Cadeia da Polimerase , RNA de Plantas/genética , Análise de Sequência de DNA , Estresse Fisiológico , Nicotiana/genética , Nicotiana/fisiologiaRESUMO
The ubiquitin extension protein (uep1) gene was identified as a constitutively expressed gene in oil palm. We have isolated and characterized the 5' region of the oil palm uep1 gene, which contains an 828 bp sequence upstream of the uep1 translational start site. Construction of a pUEP1 transformation vector, which contains gusA reporter gene under the control of uep1 promoter, was carried out for functional analysis of the promoter through transient expression studies. It was found that the 5' region of uep1 functions as a constitutive promoter in oil palm and could drive GUS expression in all tissues tested, including embryogenic calli, embryoid, immature embryo, young leaflet from mature palm, green leaf, mesocarp and meristematic tissues (shoot tip). This promoter could also be used in dicot systems as it was demonstrated to be capable of driving gusA gene expression in tobacco.