Genomic predictions for crossbred dairy cattle.

Genomic evaluations are useful for crossbred as well as purebred populations when selection is applied to commercial herds. Dairy farmers had already spent more than $1 million to genotype over 32,000 crossbred animals before US genomic evaluations became available for those animals. Thus, new tools were needed to provide accurate genomic predictions for crossbreds. Genotypes for crossbreds are imputed more accurately when the imputation reference population includes purebreds. Therefore, genotypes of 6,296 crossbred animals were imputed from lower-density chips by including either 3,119 ancestors or 834,367 genotyped animals in the reference population. Crossbreds in the imputation study included 733 Jersey × Holstein F1 animals, 55 Brown Swiss × Holstein F1 animals, 2,300 Holstein backcrosses, 2,026 Jersey backcrosses, 27 Brown Swiss backcrosses, and 502 other crossbreds of various breed combinations. Another 653 animals appeared to be purebreds that owners had miscoded as a different breed. Genomic breed composition was estimated from 60,671 markers using the known breed identities for purebred, progeny-tested Holstein, Jersey, Brown Swiss, Ayrshire, and Guernsey bulls as the 5 traits (breed fractions) to be predicted. Estimates of breed composition were adjusted so that no percentages were negative or exceeded 100%, and breed percentages summed to 100%. Another adjustment set percentages above 93.5% equal to 100%, and the resulting value was termed breed base representation (BBR). Larger percentages of missing alleles were imputed by using a crossbred reference population rather than only the closest purebred reference population. Crossbred predictions were averages of genomic predictions computed using marker effects for each pure breed, which were weighted by the animal's BBR. Marker and polygenic effects were estimated separately for each breed on the all-breed scale instead of within-breed scales. For crossbreds, genomic predictions weighted by BBR were more accurate than the average of parents' breeding values and slightly more accurate than predictions using only the predominant breed. For purebreds, single-trait predictions using only within-breed data were as accurate as multi-trait predictions with allele effects in different breeds treated as correlated effects. Crossbred genomic predicted transmitting abilities were implemented by the Council on Dairy Cattle Breeding in April 2019 and will aid producers in managing their breeding programs and selecting replacement heifers.

Genome-wide association study for ketosis in US Jerseys using producer-recorded data.

Ketosis is one of the most frequently reported metabolic health events in dairy herds. Several genetic analyses of ketosis in dairy cattle have been conducted; however, few have focused specifically on Jersey cattle. The objectives of this research included estimating variance components for susceptibility to ketosis and identification of genomic regions associated with ketosis in Jersey cattle. Voluntary producer-recorded health event data related to ketosis were available from Dairy Records Management Systems (Raleigh, NC). Standardization was implemented to account for the various acronyms used by producers to designate an incidence of ketosis. Events were restricted to the first reported incidence within 60 d after calving in first through fifth parities. After editing, there were a total of 42,233 records from 23,865 cows. A total of 1,750 genotyped animals were used for genomic analyses using 60,671 markers. Because of the binary nature of the trait, a threshold animal model was fitted using THRGIBBS1F90 (version 2.110) using only pedigree information, and genomic information was incorporated using a single-step genomic BLUP approach. Individual single nucleotide polymorphism (SNP) effects and the proportion of variance explained by 10-SNP windows were calculated using postGSf90 (version 1.38). Heritability of susceptibility to ketosis was 0.083 [standard deviation (SD) = 0.021] and 0.078 (SD = 0.018) in pedigree-based and genomic analyses, respectively. The marker with the largest associated effect was located on chromosome 10 at 66.3 Mbp. The 10-SNP window explaining the largest proportion of variance (0.70%) was located on chromosome 6 beginning at 56.1 Mbp. Gene Ontology (GO) and Medical Subject Heading (MeSH) enrichment analyses identified several overrepresented processes and terms related to immune function. Our results indicate that there is a genetic component related to ketosis susceptibility in Jersey cattle and, as such, genetic selection for improved resistance to ketosis is feasible.

Genetic evaluations for mixed-breed populations.

An all-breed animal model was developed for routine genetic evaluations of US dairy cattle. Data sets from individual breeds were combined, and records from crossbred cows were included. About 1% of recent cows were first-generation crossbreds. The numbers of cows with records since 1960 ranged from 10 to 22 million for the 6 traits analyzed, which were milk, fat, protein, somatic cell score, productive life, and daughter pregnancy rate. Programs were modified to account for general heterosis, to group unknown parents separately by breed, to adjust variances separately by breed, and to adjust data to a 36-mo age equivalent instead of a mature equivalent. Convergence rate of the all-breed model was similar to that of the previous within-breed animal model. Estimated breed differences were similar to those obtained previously from phenotypic breed means or from studies of crossbred cows and their herd-mates. Genetic evaluations from the all-breed and within-breed systems had high correlations: >0.99 for recent Holsteins and slightly <0.99 for other breeds. Predicted transmitting abilities will be converted back to the within-breed bases for purebred animals and to the breed of sire base for crossbred animals so that most purebred breeders will not be affected by the change to a multibreed model. Evaluations of crossbred animals from the multibreed model can include accurate information for both parents. Reliabilities also increase for purebred relatives because of the additional crossbred records and in mixed breed herds because cows of other breeds are additional contemporaries. Another benefit of the multibreed model is that breed differences are routinely estimated and updated. More research and education may be needed on using the new evaluations in the design of breeding programs. Implementation is expected in May 2007.