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1.
J Gen Virol ; 98(8): 1999-2000, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28786782

RESUMO

The family Virgaviridae is a family of plant viruses with rod-shaped virions, a ssRNA genome with a 3'-terminal tRNA-like structure and a replication protein typical of alpha-like viruses. Differences in the number of genome components, genome organization and the mode of transmission provide the basis for genus demarcation. Tobacco mosaic virus (genus Tobamovirus) was the first virus to be discovered (in 1886); it is present in high concentrations in infected plants, is extremely stable and has been extensively studied. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the taxonomy of the Virgaviridae, which is available at www.ictv.global/report/virgaviridae.


Assuntos
Vírus de Plantas/classificação , Genoma Viral , Doenças das Plantas/virologia , Vírus de Plantas/genética , Vírus de Plantas/isolamento & purificação , Vírus de Plantas/fisiologia , Plantas/virologia , RNA Viral/genética
2.
Phytopathology ; 106(6): 532-40, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-26976729

RESUMO

The U.S. Culture Collection Network was formed in 2012 by a group of culture collection scientists and stakeholders in order to continue the progress established previously through efforts of an ad hoc group. The network is supported by a Research Coordination Network grant from the U.S. National Science Foundation (NSF) and has the goals of promoting interaction among collections, encouraging the adoption of best practices, and protecting endangered or orphaned collections. After prior meetings to discuss best practices, shared data, and synergy with genome programs, the network held a meeting at the U.S. Department of Agriculture (USDA)-Agricultural Research Service (ARS) National Center for Genetic Resources Preservation (NCGRP) in Fort Collins, Colorado in October 2015 specifically to discuss collections that are vulnerable because of changes in funding programs, or are at risk of loss because of retirement or lack of funding. The meeting allowed collection curators who had already backed up their resources at the USDA NCGRP to visit the site, and brought collection owners, managers, and stakeholders together. Eight formal collections have established off-site backups with the USDA-ARS, ensuring that key material will be preserved for future research. All of the collections with backup at the NCGRP are public distributing collections including U.S. NSF-supported genetic stock centers, USDA-ARS collections, and university-supported collections. Facing the retirement of several pioneering researchers, the community discussed the value of preserving personal research collections and agreed that a mechanism to preserve these valuable collections was essential to any future national culture collection system. Additional input from curators of plant and animal collections emphasized that collections of every kind face similar challenges in developing long-range plans for sustainability.


Assuntos
Bactérias/genética , Genômica/organização & administração , Microbiologia/organização & administração , Agricultura , Bactérias/classificação , Bases de Dados Factuais/legislação & jurisprudência , Estados Unidos , United States Department of Agriculture/organização & administração
3.
PLoS Pathog ; 9(8): e1003337, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23950706

RESUMO

Human activity is causing new encounters between viruses and plants. Anthropogenic interventions include changing land use, decreasing biodiversity, trade, the introduction of new plant and vector species to native landscapes, and changing atmospheric and climatic conditions. The discovery of thousands of new viruses, especially those associated with healthy-appearing native plants, is shifting the paradigm for their role within the ecosystem from foe to friend. The cost of new plant virus incursions can be high and result in the loss of trade and/or production for short or extended periods. We present and justify three recommendations for plant biosecurity to improve communication about plant viruses, assist with the identification of viruses and their impacts, and protect the high economic, social, environmental, and cultural value of our respective nations' unique flora: 1) As part of the burden of proof, countries and jurisdictions should identify what pests already exist in, and which pests pose a risk to, their native flora; 2) Plant virus sequences not associated with a recognized virus infection are designated as "uncultured virus" and tentatively named using the host plant species of greatest known prevalence, the word "virus," a general location identifier, and a serial number; and 3) Invest in basic research to determine the ecology of known and new viruses with existing and potential new plant hosts and vectors and develop host-virus pathogenicity prediction tools. These recommendations have implications for researchers, risk analysts, biosecurity authorities, and policy makers at both a national and an international level.


Assuntos
Temas Bioéticos , Pesquisa Biomédica , Doenças das Plantas , Vírus de Plantas , Humanos
4.
Arch Virol ; 159(7): 1755-64, 2014 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-24519459

RESUMO

The Plant Virus Biodiversity and Ecology project was undertaken to better understand the nature of plant-viral interactions and the occurrence of non-pathogenic viruses. Plants from the Tallgrass Prairie Preserve (TPP), Osage County, Oklahoma, were surveyed from 2005 to 2008 for the presence of viruses, resulting in the detection, using a virus-like particle enrichment method, of the genome a novel virus, Ambrosia asymptomatic virus 1 (AAV1), from Ambrosia psilostachya DC (western ragweed). Here, we present the genomic organization and genetic variability of AAV1. The virus has a single-stranded RNA genome of about 7408 nt, which has six open reading frames (ORFs). Phylogenetic analysis of the replicase and coat protein ORFs of the virus indicates strongly that the virus should be placed in the genus Mandarivirus. No evidence of recombination was detected. We also report the detection in the TPP of two known viruses and seven other putative viruses, members of the order Tymovirales.


Assuntos
Ambrosia/virologia , Flexiviridae/isolamento & purificação , Genoma Viral/genética , Plantas/virologia , Tymoviridae/genética , Tymoviridae/isolamento & purificação , Sequência de Bases , Flexiviridae/classificação , Flexiviridae/genética , Regulação Viral da Expressão Gênica , Variação Genética , Oklahoma , Fases de Leitura Aberta , Filogenia , RNA Viral/genética , Tymoviridae/classificação
5.
BMC Microbiol ; 13: 1, 2013 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-23286760

RESUMO

BACKGROUND: Plant endophytic bacteria play an important role benefiting plant growth or being pathogenic to plants or organisms that consume those plants. Multiple species of bacteria have been found co-inhabiting plants, both cultivated and wild, with viruses and fungi. For these reasons, a general understanding of plant endophytic microbial communities and their diversity is necessary. A key issue is how the distributions of these bacteria vary with location, with plant species, with individual plants and with plant growing season. RESULTS: Five common plant species were collected monthly for four months in the summer of 2010, with replicates from four different sampling sites in the Tallgrass Prairie Preserve in Osage County, Oklahoma, USA. Metagenomic DNA was extracted from ground, washed plant leaf samples, and fragments of the bacterial 16S rDNA genes were amplified for analysis of terminal restriction fragment length polymorphism (T-RFLP). We performed mono-digestion T-RFLP with restriction endonuclease DdeI, to reveal the structures of leaf endophytic bacterial communities, to identify the differences between plant-associated bacterial communities in different plant species or environments, and to explore factors affecting the bacterial distribution. We tested the impacts of three major factors on the leaf endophytic bacterial communities, including host plant species, sampling dates and sampling locations. CONCLUSIONS: Results indicated that all of the three factors were significantly related (α = 0.05) to the distribution of leaf endophytic bacteria, with host species being the most important, followed by sampling dates and sampling locations.


Assuntos
Bactérias/classificação , Biodiversidade , Endófitos/classificação , Metagenoma , Folhas de Planta/microbiologia , Polimorfismo de Fragmento de Restrição , Bactérias/genética , Impressões Digitais de DNA , DNA Bacteriano/genética , DNA Ribossômico/genética , Endófitos/genética , Oklahoma , RNA Ribossômico 16S/genética
6.
Phytopathology ; 103(2): 129-34, 2013 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-23013451

RESUMO

Corn stunt disease has become a factor limiting maize production in some areas of the Americas in recent years. Although resistant maize genotypes have been developed in the past, this resistance has been unstable over time or in some geographical locations. To better understand disease components that could affect the stability of host resistance, we assessed the genome variability of the etiologic agent, Spiroplasma kunkelii. Isolates were obtained from a number of areas, and characterized molecularly by amplification of several regions of the spiroplasma chromosome and sequencing of specific gene fragments. The degree of polymorphism between isolates of different geographic origins was low, and the level of genomic variability was similar within isolates of different countries. Polymorphism among isolates was found in viral insertions and in the sequence of Skarp, a gene that encodes a membrane protein implicated in attachment to insect cells. The results suggest that the genome composition of this species is highly conserved among isolates. Hence, it is unlikely that the instability of maize resistance is due to generation of new pathotypes of S. kunkelii. Instead, other components of this complex pathosystem could account for the breakdown of resistance.


Assuntos
Genoma Bacteriano/genética , Doenças das Plantas/microbiologia , Polimorfismo Genético/genética , Spiroplasma/genética , Zea mays/microbiologia , Argentina , Proteínas de Bactérias/genética , Brasil , Costa Rica , DNA Bacteriano/química , Resistência à Doença , Genótipo , Geografia , México , Filogenia , Folhas de Planta/microbiologia , Análise de Sequência de DNA , Spiroplasma/isolamento & purificação , Estados Unidos
7.
BMC Bioinformatics ; 13 Suppl 15: S9, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-23046503

RESUMO

BACKGROUND: Members of the phylum Proteobacteria are most prominent among bacteria causing plant diseases that result in a diminution of the quantity and quality of food produced by agriculture. To ameliorate these losses, there is a need to identify infections in early stages. Recent developments in next generation nucleic acid sequencing and mass spectrometry open the door to screening plants by the sequences of their macromolecules. Such an approach requires the ability to recognize the organismal origin of unknown DNA or peptide fragments. There are many ways to approach this problem but none have emerged as the best protocol. Here we attempt a systematic way to determine organismal origins of peptides by using a machine learning algorithm. The algorithm that we implement is a Support Vector Machine (SVM). RESULT: The amino acid compositions of proteobacterial proteins were found to be different from those of plant proteins. We developed an SVM model based on amino acid and dipeptide compositions to distinguish between a proteobacterial protein and a plant protein. The amino acid composition (AAC) based SVM model had an accuracy of 92.44% with 0.85 Matthews correlation coefficient (MCC) while the dipeptide composition (DC) based SVM model had a maximum accuracy of 94.67% and 0.89 MCC. We also developed SVM models based on a hybrid approach (AAC and DC), which gave a maximum accuracy 94.86% and a 0.90 MCC. The models were tested on unseen or untrained datasets to assess their validity. CONCLUSION: The results indicate that the SVM based on the AAC and DC hybrid approach can be used to distinguish proteobacterial from plant protein sequences.


Assuntos
Proteínas de Bactérias/classificação , Proteínas de Plantas/classificação , Proteobactérias/química , Máquina de Vetores de Suporte , Sequência de Aminoácidos , Bases de Dados de Proteínas , Modelos Teóricos
8.
J Gen Virol ; 93(Pt 2): 408-418, 2012 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-22049092

RESUMO

The proposed phylogenetic structure of the genus Tobamovirus supports the idea that these viruses have codiverged with their hosts since radiation of the hosts from a common ancestor. The determinations of genome sequence for two strains of Passion fruit mosaic virus (PafMV), a tobamovirus from plants of the family Passifloraceae (order Malpighiales) from which only one other tobamovirus (Maracuja mosaic virus; MarMV) has been characterized, combined with the development of Bayesian analysis methods for phylogenetic inference, provided an opportunity to reassess the co-divergence hypothesis. The sequence of one PafMV strain, PfaMV-TGP, was discovered during a survey of plants of the Tallgrass Prairie Preserve for their virus content. Its nucleotides are only 73 % identical to those of MarMV. A conserved ORF not found in other tobamovirus genomes, and encoding a cysteine-rich protein, was found in MarMV and both PafMV strains. Phylogenetic tree construction, using an alignment of the nucleotide sequences of PafMV-TGP and other tobamoviruses resulted in a major clade containing isolates exclusively from rosid plants. Asterid-derived viruses were exclusively found in a second major clade that also contained an orchid-derived tobamovirus and tobamoviruses infecting plants of the order Brassicales. With a few exceptions, calibrating the virus tree with dates of host divergence at two points resulted in predictions of divergence times of family specific tobamovirus clades that were consistent with the times of divergence of the host plant orders.


Assuntos
Evolução Biológica , Filogenia , Plantas/virologia , Tobamovirus/genética , Tobamovirus/isolamento & purificação , Análise por Conglomerados , Sequência Conservada , Genoma Viral , Genótipo , Dados de Sequência Molecular , Fases de Leitura Aberta , Análise de Sequência de DNA , Homologia de Sequência do Ácido Nucleico
9.
Arch Virol ; 157(10): 1919-29, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-22729615

RESUMO

Population genetic analysis of grapevine fanleaf virus (GFLV) was done on the basis of the virus movement protein (MP) gene sequences from the isolates detected and identified in this study and those of all previously reported GFLV strains/isolates. These revealed that the GFLV populations of Iran and Slovenia were highly distinct, whereas those of France, Germany, Italy and the USA were composed of multiple lineages. All populations were significantly differentiated from each other. However, two GFLV isolates from Tunisia, the only recorded GFLVs from that country, were not statistically distinct from the French, German and Italian populations. The ratio of non-synonymous nucleotide diversity to synonymous nucleotide diversity (Pi(a)/Pi(s)) was less than 1, suggesting that the MP gene has been under purifying selection. The neutrality tests were indicative of a balancing selection that is operating within Iranian and USA GFLV isolates, but they show a purifying selection within the other populations. Eleven recombination events were detected in a total of 50 isolates from France, Germany, Iran, Italy, Slovenia and the USA. The results from the recombination analysis were in agreement with those of the phylogenetic analysis. This study suggests that diversity among GFLV geographical populations resulted from possible host adaptation, recombination and founder effects.


Assuntos
Nepovirus/classificação , Nepovirus/genética , Doenças das Plantas/virologia , Proteínas do Movimento Viral em Plantas/genética , Recombinação Genética , Vitis/virologia , França , Variação Genética , Genética Populacional , Alemanha , Irã (Geográfico) , Itália , Dados de Sequência Molecular , Nepovirus/isolamento & purificação , Filogenia , Proteínas do Movimento Viral em Plantas/química , Análise de Sequência de DNA , Eslovênia , Tunísia , Estados Unidos
10.
Phytopathology ; 102(2): 166-76, 2012 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-22026416

RESUMO

Native virus-plant interactions require more understanding and their study will provide a basis from which to identify potential sources of emerging destructive viruses in crops. A novel tymovirus sequence was detected in Asclepias viridis (green milkweed), a perennial growing in a natural setting in the Tallgrass Prairie Preserve (TGPP) of Oklahoma. It was abundant within and frequent among A. viridis plants and, to varying extents, within other dicotyledonous and one grass (Panicum virgatum) species obtained from the TGPP. Extracts from A. viridis containing the sequence were infectious to a limited number of species. The virus genome was cloned and determined to be closely related to Kennedya yellow mosaic virus. The persistence of the virus within the Oklahoma A. viridis population was monitored for five successive years. Virus was present in a high percentage of plants within representative areas of the TGPP in all years and was spreading to additional plants. Virus was present in regions adjacent to the TGPP but not in plants sampled from central and south-central Oklahoma. Virus was present in the underground caudex of the plant during the winter, suggesting overwintering in this tissue. The RNA sequence encoding the virus coat protein varied considerably between individual plants (≈3%), likely due to drift rather than selection. An infectious clone was constructed and the virus was named Asclepias asymptomatic virus (AsAV) due to the absence of obvious symptoms on A. viridis.


Assuntos
Asclepias/virologia , Genoma Viral/genética , Doenças das Plantas/virologia , Tymovirus/isolamento & purificação , Sequência de Bases , Proteínas do Capsídeo/genética , Clonagem Molecular , DNA Complementar/química , DNA Complementar/genética , Deriva Genética , Variação Genética , Geografia , Especificidade de Hospedeiro , Interações Hospedeiro-Patógeno , Dados de Sequência Molecular , Oklahoma , Filogenia , Folhas de Planta/virologia , RNA Viral/genética , Análise de Sequência de DNA , Nicotiana/virologia , Tymovirus/classificação , Tymovirus/genética
11.
Can J Microbiol ; 57(6): 525-32, 2011 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21635220

RESUMO

Phytopathogenic spiroplasmas can multiply in vascular plants and insects. A deeper understanding of this dual-host life could be furthered through the identification by random mutagenesis of spiroplasma genes required. The ability of the EZ::TN™ Tnp transposome™ system to create random insertional mutations in the genome of Spiroplasma citri was evaluated. The efficiency of electroporation-mediated transformation of S. citri BR3-3X averaged 28.8 CFUs/ng transposome for 10(9) spiroplasma cells. Many transformants appearing on the selection plates were growth impaired when transferred to broth. Altering broth composition in various ways did not improve their growth. However, placing colonies into a small broth volume resulted in robust growth and successful subsequent passages of a subset of transformants. PCR using primers for the dihydrofolate reductase gene confirmed the transposon's presence in the genomes of selected transformants. Southern blot hybridization and nucleotide sequencing suggested that insertion was random within the chromosome and usually at single sites. The insertions were stable. Growth rates of all transformants were lower than that of the wild-type S. citri, but none lost the ability to adhere to a Circulifer tenellus (CT-1) cell line. The EZ::TN™ Tnp transposome™ system represents an additional tool for genetic manipulation of the fastidious spiroplasmas.


Assuntos
Técnicas Bacteriológicas/métodos , Mutagênese Insercional , Spiroplasma citri/crescimento & desenvolvimento , Spiroplasma citri/genética , Animais , Linhagem Celular , Meios de Cultura , DNA Bacteriano/genética , Eletroporação , Genoma Bacteriano , Hemípteros/microbiologia , Viabilidade Microbiana , Reação em Cadeia da Polimerase , Análise de Sequência de DNA , Transformação Genética
12.
BMC Bioinformatics ; 11 Suppl 6: S3, 2010 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-20946614

RESUMO

BACKGROUND: Phylogenetic analyses reveal probable patterns of divergence of present day organisms from common ancestors. The points of divergence of lineages can be dated if a corresponding historical or fossil record exists. For many species, in particular viruses, such records are rare. Recently, Bayesian phylogenetic analysis using sequences from closely related organisms isolated at different times have been used to calibrate divergences. Phylogenetic analyses depend on the assumption that the average substitution rates that can be calculated from the data apply throughout the course of evolution. RESULTS: The present study tests this crucial assumption by charting the kinds of substitutions observed between pairs of sequences with different levels of total substitutions. Datasets of aligned sequences, both viral and non-viral, were assembled. For each pair of sequences in an aligned set, the distribution of nucleotide interchanges and the total number of changes were calculated. Data were binned according to total numbers of changes and plotted. The accumulation of the six possible interchange types in retroelements as a function of distance followed closely the expected hyperbolic relationship. For other datasets, however, significant deviations from this relationship were noted. A rapid initial accumulation of transition interchanges was frequent among the datasets and anomalous changes occurred at specific divergence levels. CONCLUSIONS: The accumulation profiles suggested that substantial changes in frequencies of types of substitutions occur over the course of evolution and that such changes should be considered in evaluating and dating viral phylogenies.


Assuntos
Evolução Molecular , Genômica/métodos , Vírus/genética , Sequência de Bases , Bases de Dados Genéticas , Filogenia
13.
Plant Dis ; 94(1): 75-82, 2010 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30754385

RESUMO

The impact of citrus stubborn disease, caused by Spiroplasma citri, on citrus production is associated with the symptom severity of infected trees but its association with bacterial levels and virulence are unknown. Fifty-eight S. citri isolates were cultivated from severely and mildly symptomatic trees and randomly amplified polymorphic DNA and short-sequence repeat fingerprinting differentiated four major S. citri genotypes among these isolates. Each genotype was present in both mildly and severely symptomatic trees, suggesting that readily detectable genetic differences in the S. citri populations did not account for differences in disease severity. No variation in the size of amplicons of the pathogenicity-related fructose operon was observed in isolates from trees having varying degrees of symptom expression. Quantitative polymerase chain reaction demonstrated that spiroplasma titer is over 6,000 times higher in fruit from severely symptomatic than from mildly symptomatic trees. The genotypic similarities among S. citri isolates from severely and mildly symptomatic trees, and the consistently higher bacterial titer in the former than in the latter, suggests that titer but not genotype is, at least in part, responsible for the greater symptom severity in some of the S. citri-affected trees in the orchard evaluated.

14.
Virus Res ; 141(2): 169-73, 2009 May.
Artigo em Inglês | MEDLINE | ID: mdl-19152821

RESUMO

The diversity of viruses associated with non-cultivated plants was assessed from plant samples collected in the Tallgrass Prairie Preserve of northeastern Oklahoma, USA. The samples were processed to determine the sequences of nucleic acids extracted from the virus-like particle fraction of plant homogenates. Sequences from 95 specimens of 52 plant species included those of probable origin from the genomes of plants (including retroelements), bacteria, fungi, other organisms, and viruses. Virus-like sequences were identified in sequences from 25% of the specimens, coming from 19% of the plant species. Evidence of a member of the genus Tymovirus was found in 16 specimens of 6 plant species, making it the most predominant virus associated with the sampled plants. There was evidence of the presence of more than one virus in each of six specimens.


Assuntos
Conservação dos Recursos Naturais , Vírus de Plantas/isolamento & purificação , Poaceae/virologia , Dados de Sequência Molecular , Oklahoma , Vírus de Plantas/classificação , Vírus de Plantas/genética
15.
Viruses ; 11(8)2019 07 24.
Artigo em Inglês | MEDLINE | ID: mdl-31344791

RESUMO

I thank all the teams of authors, the scientists who reviewed submitted manuscripts and made suggestions that improved the reports, and the editorial staff workers who put this special issue together [...].


Assuntos
Biodiversidade , Ecologia , Vírus de Plantas/genética , Vírus de Plantas/classificação , Vírus de Plantas/fisiologia
16.
Viruses ; 11(5)2019 05 05.
Artigo em Inglês | MEDLINE | ID: mdl-31060293

RESUMO

Diverse studies of viral evolution have led to the recognition that the evolutionary rates of viral taxa observed are dependent on the time scale being investigated-with short-term studies giving fast substitution rates, and orders of magnitude lower rates for deep calibrations. Although each of these factors may contribute to this time dependent rate phenomenon, a more fundamental cause should be considered. We sought to test computationally whether the basic phenomena of virus evolution (mutation, replication, and selection) can explain the relationships between the evolutionary and phylogenetic distances. We tested, by computational inference, the hypothesis that the phylogenetic distances between the pairs of sequences are functions of the evolutionary path lengths between them. A Basic simulation revealed that the relationship between simulated genetic and mutational distances is non-linear, and can be consistent with different rates of nucleotide substitution at different depths of branches in phylogenetic trees.


Assuntos
Evolução Molecular , Mutação , Vírus/genética , Humanos , Filogenia , Viroses/virologia , Vírus/classificação
17.
BMC Evol Biol ; 8: 335, 2008 Dec 18.
Artigo em Inglês | MEDLINE | ID: mdl-19094195

RESUMO

BACKGROUND: Viruses that have spent most of their evolutionary time associated with a single host lineage should have sequences that reflect codivergence of virus and host. Several examples for RNA viruses of host-virus tree congruence are being challenged. DNA viruses, such as mastreviruses, are more likely than RNA viruses to have maintained a record of host lineage association. RESULTS: The full genomes of 28 isolates of Wheat dwarf virus (WDV), a member of the Mastrevirus genus, from different regions of China were sequenced. The analysis of these 28 entire genomes and 18 entire genome sequences of cereal mastreviruses from other countries support the designation of wheat, barley and oat mastrevirus isolates as separate species. They revealed that relative divergence times for the viruses WDV, Barley dwarf virus (BDV), Oat dwarf virus (ODV) and Maize streak virus (MSV) are proportional to divergence times of their hosts, suggesting codivergence. Considerable diversity among Chinese isolates was found and was concentrated in hot spots in the Rep A, SIR, LIR, and intron regions in WDV genomes. Two probable recombination events were detected in Chinese WDV isolates. Analysis including further Mastrevirus genomes concentrated on coding regions to avoid difficulties due to recombination and hyperdiversity. The analysis demonstrated congruence of trees in two branches of the genus, but not in the third. Assuming codivergence, an evolutionary rate of 10-8 substitutions per site per year was calculated. The low rate implies stronger constraints against change than are obtained by other methods of estimating the rate. CONCLUSION: We report tests of the hypothesis that mastreviruses have codiverged with their monocotyledonous hosts over 50 million years of evolution. The tests support the hypothesis for WDV, BDV and ODV, but not for MSV and other African streak viruses.


Assuntos
Evolução Molecular , Geminiviridae/genética , Interações Hospedeiro-Patógeno , Doenças das Plantas/virologia , Poaceae/virologia , Geminiviridae/classificação , Geminiviridae/isolamento & purificação , Dados de Sequência Molecular , Filogenia , Recombinação Genética , Alinhamento de Sequência , Análise de Sequência de DNA
18.
J Virol Methods ; 152(1-2): 49-55, 2008 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-18590770

RESUMO

To test the hypothesis that many viruses remain to be discovered in plants, a procedure was developed to sequence nucleic acids cloned randomly from virus-like particle fractions of plant homogenates. As a test of the efficiency of the procedure we targeted Ambrosia psilostachya, western ragweed, plants growing at the Tallgrass Prairie Preserve of northeastern Oklahoma. Amplifiable nucleic acid was found in the fractions from six of twelve specimens and sequences were characterized from four of them. Evidence was obtained for the presence of viruses belonging to two families (Caulimoviridae, Flexiviridae). Multiple viral species were found in two of the four specimens and their level within the isolated nucleic acid population varied from less than 1-37%. None of the sequences were derived from reported sequences of known viruses. Thus, the analysis of nucleic acid from virus-like particles is a useful tool to expand our knowledge of the universe of viruses to non-cultivated species.


Assuntos
Ambrosia/virologia , DNA Viral/genética , RNA Viral/genética , Vírus/isolamento & purificação , DNA Viral/análise , Dados de Sequência Molecular , RNA Viral/análise , Vírus/classificação , Vírus/genética
19.
PLoS One ; 13(11): e0207062, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30403741

RESUMO

Coniothyrium glycines, the causal agent of soybean red leaf blotch, is a USDA APHIS-listed Plant Pathogen Select Agent and potential threat to US agriculture. Sequencing of the C. glycines mt genome revealed a circular 98,533-bp molecule with a mean GC content of 29.01%. It contains twelve of the mitochondrial genes typically involved in oxidative phosphorylation (atp6, cob, cox1-3, nad1-6, and nad4L), one for a ribosomal protein (rps3), four for hypothetical proteins, one for each of the small and large subunit ribosomal RNAs (rns and rnl) and a set of 30 tRNAs. Genes were encoded on both DNA strands with cox1 and cox2 occurring as adjacent genes having no intergenic spacers. Likewise, nad2 and nad3 are adjacent with no intergenic spacers and nad5 is immediately followed by nad4L with an overlap of one base. Thirty-two introns, comprising 54.1% of the total mt genome, were identified within eight protein-coding genes and the rnl. Eighteen of the introns contained putative intronic ORFs with either LAGLIDADG or GIY-YIG homing endonuclease motifs, and an additional eleven introns showed evidence of truncated or degenerate endonuclease motifs. One intron possessed a degenerate N-acetyl-transferase domain. C. glycines shares some conservation of gene order with other members of the Pleosporales, most notably nad6-rnl-atp6 and associated conserved tRNA clusters. Phylogenetic analysis of the twelve shared protein coding genes agrees with commonly accepted fungal taxonomy. C. glycines represents the second largest mt genome from a member of the Pleosporales sequenced to date. This research provides the first genomic information on C. glycines, which may provide targets for rapid diagnostic assays and population studies.


Assuntos
Ascomicetos/genética , Ascomicetos/fisiologia , Endonucleases/metabolismo , Genoma Mitocondrial/genética , Glycine max/microbiologia , Anotação de Sequência Molecular , Doenças das Plantas/microbiologia , Códon/genética , Endonucleases/genética , Genômica , Íntrons/genética , RNA de Transferência/genética
20.
mBio ; 8(4)2017 08 15.
Artigo em Inglês | MEDLINE | ID: mdl-28811341

RESUMO

The U.S. Culture Collection Network held a meeting to share information about how culture collections are responding to the requirements of the recently enacted Nagoya Protocol on Access to Genetic Resources and the Fair and Equitable Sharing of Benefits Arising from their Utilization to the Convention on Biological Diversity (CBD). The meeting included representatives of many culture collections and other biological collections, the U.S. Department of State, U.S. Department of Agriculture, Secretariat of the CBD, interested scientific societies, and collection groups, including Scientific Collections International and the Global Genome Biodiversity Network. The participants learned about the policies of the United States and other countries regarding access to genetic resources, the definition of genetic resources, and the status of historical materials and genetic sequence information. Key topics included what constitutes access and how the CBD Access and Benefit-Sharing Clearing-House can help guide researchers through the process of obtaining Prior Informed Consent on Mutually Agreed Terms. U.S. scientists and their international collaborators are required to follow the regulations of other countries when working with microbes originally isolated outside the United States, and the local regulations required by the Nagoya Protocol vary by the country of origin of the genetic resource. Managers of diverse living collections in the United States described their holdings and their efforts to provide access to genetic resources. This meeting laid the foundation for cooperation in establishing a set of standard operating procedures for U.S. and international culture collections in response to the Nagoya Protocol.


Assuntos
Biodiversidade , Bancos de Espécimes Biológicos , Biotecnologia/legislação & jurisprudência , Microbiologia Ambiental , Agricultura/legislação & jurisprudência , Agricultura/organização & administração , Bancos de Espécimes Biológicos/legislação & jurisprudência , Bancos de Espécimes Biológicos/organização & administração , Biotecnologia/organização & administração , Bases de Dados Genéticas/legislação & jurisprudência , Modelos Genéticos , Estados Unidos , United States Department of Agriculture
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