Cadmium effects on the freshwater teleost Prochilodus lineatus: Accumulation and biochemical, genotoxic, and behavioural biomarkers.

In order to evaluate the effects of Cd, juveniles of the Neotropical fish Prochilodus lineatus were exposed to 1 and 10 µg L-1 Cd, for 24 and 96 h. Fish exposed to Cd showed metal accumulation in the gills, kidney, and liver, an increase in DNA damage in erythrocytes, and an increase in lipid peroxidation (LPO) in the kidney. Cd exposure also caused a reduction in catalase activity, metallothionein induction, and LPO in the liver. Cd stimulated the swimming activity of exposed fish, resulting in longer swimming times and distances travelled, especially for the shortest exposure time. Changes in acetylcholinesterase activity (AChE) in the muscle and brain are probably related to these behavioural responses. These results show that Cd affects the functioning of several organs in P. lineatus, which is indicated by the genotoxic damage and changes in the AChE and swimming pattern of the exposed fish.

Multiple biomarker responses in Prochilodus lineatus subjected to short-term in situ exposure to streams from agricultural areas in Southern Brazil.

In order to assess the quality of streams susceptible to contamination by pesticides we apply biochemical and genotoxic biomarkers in the Neotropical fish Prochilodus lineatus submitted to in situ tests. Fish were caged, for 96 h, in two streams located in areas with intensive use of pesticides, the Apertados (AP) and the Jacutinga (JC), and in a small stream (Godoy stream ­ GD) found inside a forest fragment adjacent to a State Park. Biochemical parameters, such as biotransformation enzymes 7-ethoxyresorufin-O-deethylase (EROD) and glutathione-S-transferase (GST), non-protein thiols (NPSH), lipoperoxidation (LPO), protein carbonylation (PCO) and acetylcholinesterase (AChE) were evaluated in various fish organs, as well as genotoxic biomarkers (damage to DNA and occurrence of micronuclei and erythrocyte nuclear abnormalities). Samples of water and sediment were collected for analysis of metals (Cu, Cr, Pb, Ni, Mn, Cd and Zn), organochloride pesticides, and triazine and glyphosate herbicides. We observed an increase in liver GST activity in fish at AP and gill GST activity in fish at JC. An increase in liver LPO was also observed in fish exposed to AP and JC. The same animals also exhibited increased DNA damage and erythrocyte nuclear abnormalities (ENAs) compared to the fish kept in GD. A number of compounds showed concentrations higher than the permitted levels, in particular, dichlorodiphenyltrichloroethane (DDT), its metabolites dichlorodiphenyldichloroethylene (DDE) and dichlorodiphenyldichloroethane (DDD), hexachlorocyclohexanes (HCH), heptachloride, diclofluanid and aldrins. These pesticides were detected at higher concentrations in water and sediment samples from AP, followed by JC and GD. The Integrated Biomarker Response Index (IBR) indicated that AP and JC (AP: 21.7 > JC: 18.5 > GD: 12.6) have the worst environmental quality. Integrated biomarker analysis revealed that the alterations observed related well with the levels of environmental contaminants, demonstrating the effectiveness of this biomonitoring approach.