The Role of IgM Antibodies in T Cell Lymphoma Protection in a Novel Model Resembling Anaplastic Large Cell Lymphoma.

MRL/lpr mice typically succumb to immune complex-mediated nephritis within the first year of life. However, MRL/lpr mice that only secrete IgM Abs because of activation-induced deaminase deficiency (AID-/-MRL/lpr mice) experienced a dramatic increase in survival. Further crossing of these mice to those incapable of making secretory IgM (µS mice) generated mice lacking any secreted Abs but with normal B cell receptors. Both strains revealed no kidney pathology, yet Ab-deficient mice still experienced high mortality. In this article, we report Ab-deficient MRL/lpr mice progressed to high-grade T cell lymphoma that can be reversed with injection of autoreactive IgM Abs or following adoptive transfer of IgM-secreting MRL/lpr B cells. Anti-nuclear Abs, particularly anti-dsDNA IgM Abs, exhibited tumor-killing activities against a murine T cell lymphoma cell line. Passive transfers of autoreactive IgM Abs into p53-deficient mice increased survival by delaying onset of T cell lymphoma. The lymphoma originated from a double-negative aberrant T cell population seen in MRL/lpr mice and most closely resembled human anaplastic large cell lymphoma. Combined, these results strongly implicate autoreactive IgM Abs in protection against T cell lymphoma.

Impacts of the COVID-19 pandemic on household food waste behaviour: A systematic review.

Food waste has adverse economic, social, and environmental impacts and increases the prevalence of food insecurity. Panic buying at the beginning of the COVID-19 outbreak raised serious concerns about a potential rise in food waste levels and higher pressure on waste management systems. This article aims to investigate the impact of COVID-19 on food waste behaviour and the extent to which it occurs using the systematic review method. A total of 38 articles were identified and reviewed following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. The findings showed that the COVID-19 pandemic led to reductions in household food waste in most countries. Several changes in shopping and cooking behaviours, food consumption, and managing inventory and leftovers have occurred due to COVID-19. Based on these insights, we predicted that some desirable food-management habits would be retained, and others would roll back in the post-COVID-19 world. The review contributes to the food waste literature by offering a comprehensive overview of behavioural changes during the COVID-19 pandemic and future research directions.

Lysines in the lyase active site of DNA polymerase ß destabilize nonspecific DNA binding, facilitating searching and DNA gap recognition.

DNA polymerase (pol) ß catalyzes two reactions at DNA gaps generated during base excision repair, gap-filling DNA synthesis and lyase-dependent 5´-end deoxyribose phosphate removal. The lyase domain of pol ß has been proposed to function in DNA gap recognition and to facilitate DNA scanning during substrate search. However, the mechanisms and molecular interactions used by pol ß for substrate search and recognition are not clear. To provide insight into this process, a comparison was made of the DNA binding affinities of WT pol ß, pol λ, and pol µ, and several variants of pol ß, for 1-nt-gap-containing and undamaged DNA. Surprisingly, this analysis revealed that mutation of three lysine residues in the lyase active site of pol ß, 35, 68, and 72, to alanine (pol ß KΔ3A) increased the binding affinity for nonspecific DNA ∼11-fold compared with that of the WT. WT pol µ, lacking homologous lysines, displayed nonspecific DNA binding behavior similar to that of pol ß KΔ3A, in line with previous data demonstrating both enzymes were deficient in processive searching. In fluorescent microscopy experiments using mouse fibroblasts deficient in PARP-1, the ability of pol ß KΔ3A to localize to sites of laser-induced DNA damage was strongly decreased compared with that of WT pol ß. These data suggest that the three lysines in the lyase active site destabilize pol ß when bound to DNA nonspecifically, promoting DNA scanning and providing binding specificity for gapped DNA.

Supply chain resilience reactive strategies for food SMEs in coping to COVID-19 crisis.

BACKGROUND: The ability of small- and medium-sized enterprises in the food industry (FSMEs) in cultivating resilience against the COVID-19 pandemic is vital food security. However, there is limited supply chain resilience literature to guide FSMEs in overcoming disruptions caused by pandemic. SCOPE AND APPROACH: This review aims to provide a broad view of SCRes reactive strategies for FSMEs in dealing with crises in the context of COVID-19. Attention is given to the literature on resilience in other types of supply chain and situated in the context of food settings. The factors are monitored or controlled to contribute to FSME resiliency.Key findings and conclusion: Four quadrants, i.e., (1) rapid with low cost, (2) rapid with high cost, (3) slow with low cost and (4) slow with high cost, are offered based on the limitations and the time needed to react, and the strategies of each quadrant are explained in depth. This review also provides a better understanding of and guidance on reactive strategies for SCRes as options for FSMEs in dealing with the COVID-19 pandemic. This review suggests future directions as extensions based on the logical flow of this review.

A causal eco-industrial park hierarchical transition model with qualitative information: Policy and regulatory framework leads to collaboration among firms.

Eco-industrial parks promise to reduce environmental and social impacts and improve the economic performance of industrial parks. However, the transition from industrial parks to eco-industrial parks is still not well understood. This study contributes to developing valid hierarchical eco-industrial park transition attribute sets with qualitative information, as prior studies lack an exploration of the attributes in the transition of eco-industrial parks in Hungary. In nature, eco-industrial park transition attributes have causal and hierarchical interrelationships and are described with qualitative information. The assessment involves an analysis of the industrial symbiosis principles by using linguistic preferences. However, multiple attributes are involved in the assessment; therefore, this study proposes the Delphi method to develop a valid attribute set and applies fuzzy set theory to translate qualitative information into crisp values. The fuzzy decision-making trial evaluation laboratory method is used to visualize the attributes' causal interrelationships under uncertainties. The results indicate that the policy and regulatory framework leads to collaboration among firms in the eco-industrial park transition model. In practice, price reforms, management commitment, strategic planning, cognitive barriers and the integration of external information are the practical criteria for improvement. Theoretical and practical implications are also discussed.

Pervasive Ohmic contacts of monolayer 4-hT2 graphdiyne transistors.

Monolayer (ML) graphdiyne, a two-dimensional semiconductor with appropriate band gap and high carrier mobility, is a promising candidate for channel material in field effect transistors (FETs). Using density functional theory combined with non-equilibrium Green's function method, we systematically investigate the contact and transport properties of graphdiyne FETs with various electrodes, including metals (Cu, Au, Ni, Al and Ag) and MXenes (Cr2C, Ta2C and V2C). Strong interaction can be found between ML graphdiyne and the Cu, Ni and MXenes electrodes with indistinguishable band structure of ML graphdiyne, while weak or medium interaction exists in the contacts of ML graphdiyne and the Au, Al and Ag electrodes where the band structure of ML graphdiyne remains intact. Despite the different contact interactions, Ohmic contacts are generated with all considered electrode materials owing to the weak Fermi level pinning of graphdiyne. The linear I-V characteristic curve verifies the Ohmic contact between Au electrode and graphdiyne ultimately. The theoretically calculated Schottky barrier heights of graphdiyne with Cu electrode are consistent with the available experimental data. Our calculation suggests that graphdiyne is an excellent channel material of FETs forming desired Ohmic contacts with wide-ranging electrodes and thus is promising to fabricate high performance FETs.

Anisotropic interfacial properties of monolayer C2N field effect transistors.

Monolayer C2N is promising for next-generation electronic and optoelectronic applications due to its appropriate band gap and high carrier efficiency. However, relative studies have been held back due to the lack of high-quality electrode contacts. Here, we comprehensively study the electronic and transport properties of monolayer C2N with a series of electrode materials (Al, Ti, Ni, Cu, Ag, Pt, V2C, Cr2C and graphene) by using the nonequilibrium Green's function (NEGF) method combined with density functional theory (DFT). The monolayer C2N forms Ohmic contacts with the Ti/Cu/Ag electrode material in both armchair and zigzag directions, whereas Ohmic contact is only formed in the zigzag direction of the C2N-Al field effect transistor. However, the C2N-Ni, -Pt, -V2C, -Mo2C, -graphene contact systems form n-type Schottky contacts in either the armchair or zigzag direction owing to the relatively strong Fermi level pinning (the pinning factor S = 0.32 in the armchair direction and S = 0.26 in the zigzag direction). By insertion of BN or graphene between the C2N and Pt electrode in the armchair direction of contact systems, the Fermi level pinning can be effectively weakened due to the suppression of metal-induced gap states. Conspicuously, an Ohmic contact is realized in the C2N field effect transistors with the BN-Pt electrode, suggesting a possible approach to fabricating high-performance devices. Our study is conducive to selecting appropriate electrode materials for C2N-based field effect transistors.

DNA polymerase ß contains a functional nuclear localization signal at its N-terminus.

DNA polymerase ß (pol ß) requires nuclear localization to fulfil its DNA repair function. Although its small size has been interpreted to imply the absence of a need for active nuclear import, sequence and structural analysis suggests that a monopartite nuclear localization signal (NLS) may reside in the N-terminal lyase domain. Binding of this domain to Importin α1 (Impα1) was confirmed by gel filtration and NMR studies. Affinity was quantified by fluorescence polarization analysis of a fluorescein-tagged peptide corresponding to pol ß residues 2-13. These studies indicate high affinity binding, characterized by a low micromolar Kd, that is selective for the murine Importin α1 (mImpα1) minor site, with the Kd strengthening to ∼140 nM for the full lyase domain (residues 2-87). A further reduction in Kd obtains in binding studies with human Importin α5 (hImpα5), which in some cases has been demonstrated to bind small domains connected to the NLS. The role of this NLS was confirmed by fluorescent imaging of wild-type and NLS-mutated pol ß(R4S,K5S) in mouse embryonic fibroblasts lacking endogenous pol ß. Together these data demonstrate that pol ß contains a specific NLS sequence in the N-terminal lyase domain that promotes transport of the protein independent of its interaction partners. Active nuclear uptake allows development of a nuclear/cytosolic concentration gradient against a background of passive diffusion.

Left-Right Asymmetry in Spectral Characteristics of Lung Sounds Detected Using a Dual-Channel Auscultation System in Healthy Young Adults.

Though lung sounds auscultation is important for the diagnosis and monitoring of lung diseases, the spectral characteristics of lung sounds have not been fully understood. This study compared the spectral characteristics of lung sounds between the right and left lungs and between healthy male and female subjects using a dual-channel auscultation system. Forty-two subjects aged 18-22 years without smoking habits and any known pulmonary diseases participated in this study. The lung sounds were recorded from seven pairs of auscultation sites on the chest wall simultaneously. We found that in four out of seven auscultation pairs, the lung sounds from the left lung had a higher total power (PT) than those from the right lung. The PT of male subjects was higher than that of female ones in most auscultation pairs. The ratio of inspiration power to expiration power (RI/E) of lung sounds from the right lung was greater than that from the left lung at auscultation pairs on the anterior chest wall, while this phenomenon was reversed at auscultation pairs on the posterior chest wall in combined subjects, and similarly in both male and female subjects. Though the frequency corresponding to maximum power density of lung sounds (FMPD) from the left and right lungs was not significantly different, the frequency that equally divided the power spectrum of lung sounds (F50) from the left lung was significantly smaller than that from the right lung at auscultation site on the anterior and lateral chest walls, while it was significantly larger than that of from the right lung at auscultation site on the posterior chest walls. In conclusion, significant differences in the PT, FMPD, F50, and RI/E between the left and right lungs at some auscultation pairs were observed by using a dual-channel auscultation system in this study. Structural differences between the left and the right lungs, between the female and male subjects, and between anterior and posterior lungs might account for the observed differences in the spectral characteristics of lung sounds. The dual-channel auscultation system might be useful for future development of digital stethoscopes and power spectral analysis of lung sounds in patients with various kinds of cardiopulmonary diseases.

Altered Ig hypermutation pattern and frequency in complementary mouse models of DNA polymerase ζ activity.

To test the hypothesis that DNA polymerase ζ participates in Ig hypermutation, we generated two mouse models of Pol ζ function: a B cell-specific conditional knockout and a knock-in strain with a Pol ζ mutagenesis-enhancing mutation. Pol ζ-deficient B cells had a reduction in mutation frequency at Ig loci in the spleen and in Peyer's patches, whereas knock-in mice with a mutagenic Pol ζ displayed a marked increase in mutation frequency in Peyer's patches, revealing a pattern that was similar to mutations in yeast strains with a homologous mutation in the gene encoding the catalytic subunit of Pol ζ. Combined, these data are best explained by a direct role for DNA polymerase ζ in Ig hypermutation.

Altered pattern of immunoglobulin hypermutation in mice deficient in Slip-GC protein.

We recently identified a novel germinal center GTPase, SLIP-GC, that localizes to replication factories in B cells and that, when reduced, induces DNA breaks in lymphoma B cell lines in an activation-induced deaminase (AID)-dependent manner. Herein, we generated mice deficient in SLIP-GC and examined the impact of SLIP-GC deficiency in immunoglobulin hypermutation and class switch recombination, both AID-dependent mechanisms. SLIP-GC-deficient mice experienced a substantial increase in mutations at G:C base pairs at the region downstream of JH4 in the immunoglobulin heavy chain locus. This change was reflected in the overall mutation frequency, and it was associated with an increase in transitions from G:C base pairs, a hallmark of AID-mediated deamination during replication. In addition, G:C transitions at non-immunoglobulin loci also increased in these mice. Given the intracellular localization of SLIP-GC to sites of replicating DNA, these results suggest that SLIP-GC protects replicating DNA from AID-mediated deamination of cytosines in both strands.

A simple method for increasing levels of high-density lipoprotein cholesterol: a pilot study of combination aerobic- and resistance-exercise training.

Evidence suggests that physical activity has a beneficial effect of elevated high-density lipoprotein cholesterol (HDL-C) on reducing coronary artery risk. However, previous studies show contrasting results for this association between different types of exercise training (i.e., aerobic, resistance, or combined aerobic and resistance training). The aim of this study was to determine which type of exercise training is more effective in increasing HDL-C levels. Forty obese men, age 18-29 yr, were randomized into 4 groups: an aerobic-training group (n = 10), a resistance-training group (n = 10), a combined-exercise-training group (n = 10), and a control group (n = 10). After a 12-wk exercise program, anthropometrics, blood biochemical variables, and physical-fitness components were compared with the data obtained at the baseline. Multiple-regression analysis was used to evaluate the association between different types of exercise training and changes in HDL-C while adjusting for potential confounders. The results showed that with the control group as the comparator, the effects of combined-exercise training (ß = 4.17, p < .0001), aerobic training (ß = 3.65, p < .0001), and resistance training (ß = 2.10, p = .0001) were positively associated with increase in HDL-C after adjusting for potential confounders. Our findings suggested that a short-term exercise program can play an important role in increasing HDL-C levels; either aerobic or resistance training alone significantly increases the HDL-C levels, but the improvements are greatest with combined aerobic and resistance training.

Spodoptera litura larvae are attracted by HvAV-3h-infected S. litura larvae-damaged pepper leaves.

BACKGROUND: Herbivore-induced plant volatiles (HIPVs) are important self-defense outputs of pepper plants to resist insect pests. Ascoviruses are pathogenic to the larvae of most lepidopteran vegetable pests. However, whether Heliothis virescens ascovirus 3h (HvAV-3h)-infected Spodoptera litura larvae can change pepper leaf HIPVs is not well understood. RESULTS: Spodoptera litura larvae preferred S. litura-infested leaves, and this preference was stronger with longer duration of S. litura infestation. In addition, S. litura larvae significantly chose pepper leaves damaged by HvAV-3h-infected S. litura over the healthy pepper leaves. Results also showed that S. litura larvae preferred leaves mechanically damaged and treated with oral secretions from HvAV-3h infected-S. litura larvae in a simulation test. We captured the volatiles emitted by leaves under six treatments. Results showed that the volatile profile changed with the different treatments. Testing of volatile blends, prepared to the proportion released showed that the blend from simulated HvAV-3h-infected S. litura larvae-damaged plants was the most attractive to S. litura larvae. Further, we also found that some of the compounds significantly attracted S. litura larvae at specific concentrations. CONCLUSION: HvAV-3h-infected S. litura can alter the release of HIPVs in pepper plants and thus become more attractive to S. litura larvae. We speculate that this may be due to alterations in the concentration of some compounds (such as geranylacetone and prohydrojasmon) affecting the behavior of S. litura larvae. © 2023 Society of Chemical Industry.

Nexus amongst environmental regulations, carbon emission intensity and technological innovation in China's construction industry.

China's construction industry confronts with the dilemma of carbon emissions in adjusting the environmental regulations. Many studies are neglected on discovering the potential nexus amongst environmental regulations (ERs), technological innovation (TI) and CEI (CEI) and ignores the relationships amongst TI for reducing CEI. To mitigate this gap, this study bridges institutional theory to integrate the practices in the construction industry. This study applies a panel dataset on the construction industry from 30 provinces during 2004-2018 and uses it with a two-step system-generalised method of moments for analysis. The proposed method enables the prevention of the interference of the heteroscedasticity problem and improves certain analytical efficiency. The results are as a guideline for policymakers in rechecking the policies and regulations adequacy. The findings indicate that (1) the forced emission reduction effect is proven by command-and-control and market-based ERs, which can inhibit CEI; (2) voluntary ERs have an inverted U-shaped nexus with CEI; in other words, the green paradox effect shifts to the forced emission reduction effect once the intensity of voluntary ERs increases; and (3) market-based and voluntary ERs reduce CEI effectively by using TI as the mediator in construction industry.

Identifying the evolution of ecological poverty alleviation efficiency and its influencing factors: evidence from counties in Northeast China.

Ecological poverty alleviation (EPA) is an effective strategy to address the vicious circle of poverty and environmental destruction in poor areas. However, it remains controversial whether this strategy has really succeeded in this respect. Previous research investigated the impact of a certain factor on EPA, and only few studies integrated them to explore their differential effects, thereby overlooking the complexity of EPA. Therefore, this study quantified the overall efficiency of the EPA strategies of 28 poor counties in three provinces of Northeast China from 2005 to 2018 by using a super-efficiency slacks-based measure model. This model can take into account undesirable outputs; as such, it has significant advantages in measuring the coordination among economic and social development and environmental protection. The Tobit model was used to explore the factors influencing EPA efficiency. The results show that, first, the majority of counties investigated had an EPA efficiency below the overall national average. Second, as for the factors influencing EPA efficiency, it was found that (1) GDP per capita and investment in environmental governance favored EPA efficiency, as they are conducive to stimulating regional consumption dynamics and achieving green economic development; (2) science and technology expenditure and urbanization were not conducive to EPA efficiency; and (3) industrial structure and trade had insignificant effects on EPA efficiency, due to the small scale of industry and the inadequacy of the policy system. This study assessed EPA efficiency from a holistic perspective, and addressed the controversies over EPA's influencing factors, thereby providing an effective method to conduct regional EPA assessment and improve EPA performance in poor regions of China.

Resource saving and carbon footprint reduction potential of urban symbiosis strategy in express packaging waste recycling network.

The booming express delivery industry corresponds to the environmental challenges caused by massive express packaging waste (EPW). An efficient logistics network is necessary link to support EPW recycling. This study, therefore, designed a circular symbiosis network for EPW recycling based on urban symbiosis strategy. The treatment of EPW in this network includes reuse, recycling and replacing. An optimization model with multi-depot collaboration combining material flow analysis and optimization methods was developed and a hybrid non-dominated sorting genetic algorithm-II (NSGA-II) was designed as technical support for designing the circular symbiosis network while quantitatively assessing the economic and environmental benefits of the network. The results show that the designed circular symbiosis option has better resource saving and carbon footprint reduction potential than both the business as usual option and circular symbiosis option without service collaboration. In practice, the proposed circular symbiosis network can save EPW recycling costs and reduce carbon footprint. This study provides a practical guideline for the application of urban symbiosis strategies to help urban green governance and the sustainable development of express companies.

A circular waste bioeconomy development model in the Ecuadorian fishery industry: the impact of government strategy on supply chain integration and smart operations.

This study develops a set of measures to address the interrelationship among circular waste-based bioeconomy (CWBE) attributes, including those of government strategy, digital collaboration, supply chain integration, smart operations, and a green supply chain, to build a circular bioeconomy that feeds fish waste back into the economy. CWBE development is a potential solution to the problem of waste reuse in the fish supply chain; however, this potential remains untapped, and prior studies have failed to provide the criteria to guide its practices. Such an analytical framework requires qualitative assessment, which is subject to uncertainty due to the linguistic preferences of decision makers. Hence, this study adopts the fuzzy Delphi method to obtain a valid set of attributes. A fuzzy decision-making trial and evaluation was applied to address the attribute relationships and determine the driving criteria of CWBE development. The results showed that government strategies play a causal role in CWBE development and drive digital collaboration, smart operations, and supply chain integration. The findings also indicated that smart manufacturing technology, organizational policies, market enhancement, supply chain analytics, and operational innovation are drivers of waste integration from fisheries into the circular economy through waste-based bioeconomy processes.

Uncovering four domains of energy management in palm oil production: a sustainable bioenergy production trend.

This study aims to identify current and future research trends in sustainable bioenergy production. The systematic review is conducted using a social network analysis method. The data were collected from the Web of Science and Scopus database (2010-2021). Out of the 1747 articles reviewed, 100 were found to be relevant for thematic analysis. The results uncovered four domains of palm oil biodiesel production for sustainable energy management: (1) renewable energy, (2) biodiesel, (3) bioenergy, and (4) life cycle assessment. This study has proposed a sustainable bioenergy production framework based on the four main domains. The framework sheds light on the future of sustainable bioenergy production. The findings indicate the potential growth of the research topic, including sustainable bioenergy, palm oil biodiesel, energy management, and carbon emissions reduction. Future research must incorporate the energy management framework to design a sustainable energy management ecosystem strategy. In addition, the industry must comply with the international sustainability standard and sustainable development goals to manage the energy supply chain and consistency of palm oil biodiesel production.

Understanding barriers and motivations in solid waste management from Malaysian industries: a comparative analysis.

The objective of this study is to explore the similarities and differences in the barriers and motivations between the plastic and resins and food and beverages industries as these two industries are the major contributors of solid waste in Malaysia. Prior studies are lacking with regard to explaining the barriers and motivations in solid waste management from the Malaysian context. This study is focused on 10 firms from the plastics and resins industry and 9 from the food and beverages industry in Malaysia. Through Rasch measurement theory, the results indicate that the barriers of lack of skills and qualifications and lack of closed-loop control and the motivations of cost savings and a business model are performed differently. The findings further confirm that the lack of skills and qualifications is a more difficult barrier to overcome than the lack of closed-loop control, while the motivation factor of a business model is more difficult to achieve than cost savings. In terms of practical contribution, this study provides results that can help policy makers in Malaysia to close the gaps present regarding the adoption of solid waste management practices and to devise appropriate incentives. The study also supports managers of companies in regard to working on the most pressing hindering and promoting factors in the field of solid waste management.

Developing a hierarchical framework for assessing the strategic effectiveness of sustainable waste management in the Somaliland construction industry.

This study contributes to develop a hierarchical framework for assessing the strategic effectiveness of waste management in the construction industry. This study identifies a valid set of strategic effectiveness attributes of sustainable waste management (SWM) in construction. Prior studies have neglected to develop a strategic effectiveness assessment framework for SWM to identify reduce, reuse, and recycle policy initiatives that ensure waste minimization and resource recovery programs. This study utilizes the fuzzy Delphi method to screen out nonessential attributes in qualitative information. This study initially proposes a set of 75 criteria; after two rounds of assessment, consensus regarding 28 criteria is achieved among experts, and the 28 criteria are validated. Fuzzy interpretive structural modeling divides the attributes into various elements. The modeling constructs a six-level model that depicts the interrelationships among the 28 validated criteria as a hierarchical framework, and it finds and ranks the optimal drivers for practical improvement. This study integrates the best-worst method to measure the weights of different criteria in the hierarchical strategic effectiveness framework. The findings reveal that waste management operational strategy, construction site waste management performance, and the mutual coordination level are the top aspects for assessing strategic effectiveness in the hierarchical framework. In practice, the waste reduction rate, the recycling rate, water and land usage, the reuse rate, and noise and air pollution levels are identified to assist policymakers in evaluation. The theoretical and managerial implications are discussed.