Sample preparation for chromatography: an African perspective.

Africa as a continent has its unique challenges for analytical chemists in sample preparation for chromatographic analyses. The areas of agriculture, environment, food and health provide formidable challenges when it comes to method development, for example, drought can result in inadequate supplies of good quality water. The testing of water quality necessitates the development of assay methods that can be employed to not only determine the quantities of pesticides associated with malaria and tsetse fly eradication programmes, but also to monitor mycotoxins or neurotoxins. Urbanisation has also meant that endocrine disruptors such as phthalate esters need to be monitored. This review will profile some of the activities by analytical chemists practising in the African continent, who seek to address some of the challenges in sample preparation for chromatographic analyses.

Employing Solid Phase Microextraction as Extraction Tool for Pesticide Residues in Traditional Medicinal Plants.

HS-SPME was optimised using blank plant sample for analysis of organochlorine pesticides (OCPs) of varying polarities in selected medicinal plants obtained from northern part of Botswana, where OCPs such as DDT and endosulfan have been historically applied to control disease carrying vectors (mosquitos and tsetse fly). The optimised SPME parameters were used to isolate analytes from root samples of five medicinal plants obtained from Maun and Kasane, Botswana. The final analytes determination was done with a gas chromatograph equipped with GC-ECD and analyte was confirmed using electron ionisation mass spectrometer (GC-MS). Dieldrin was the only pesticide detected and confirmed with MS in the Terminalia sericea sample obtained from Kasane. The method was validated and the analyte recoveries ranged from 69.58 ± 7.20 to 113 ± 15.44%, with RSDs ranging from 1.19 to 17.97%. The method indicated good linearity (R2 > 0.9900) in the range of 2 to 100 ng g-1. The method also proved to be sensitive with low limits of detection (LODs) ranging from 0.48 ± 0.16 to 1.50 ± 0.50 ng g-1. It can be concluded that SPME was successfully utilized as a sampling and extraction tool for pesticides of diverse polarities in root samples of medicinal plants.