Different strategies of osmoadaptation in the closely related marine myxobacteria Enhygromyxa salina SWB007 and Plesiocystis pacifica SIR-1.

Only a few myxobacteria are known to date that are classified as marine, owing to their salt dependency. In this study, the salt tolerance mechanism of these bacteria was investigated. To this end, a growth medium was designed in which the mutated Escherichia coli strain BKA13 served as sole food source for the predatory, heterotrophic myxobacteria. This enabled measurement of the osmolytes without any background and revealed that the closely related strains Enhygromyxa salina SWB007 and Plesiocystis pacifica SIR-1 developed different strategies to handle salt stress. Ple. pacifica SIR-1, which was grown between 1 and 4 % NaCl, relies solely on the accumulation of amino acids, while Enh. salina SWB007, which was grown between 0.5 and 3 % NaCl, employs, besides betaine, hydroxyectoine as the major compatible solute. In accordance with this analysis, only in the latter strain was a locus identified that codes for genes corresponding to the biosynthesis of betaine, ectoine and hydroxyectoine.

Intra and extracellular effects of benzo [α] pyrene on liver, gill and blood of Caspian White fish (Rutilus frissi kutum): Cyto-genotoxicity and histopathology approach.

Southern parts of the Caspian Sea have been faced with a diverse range of oil pollutants. Therefore, this study was designed to evaluate the effects of relevant environmental concentrations of benzo[α]pyrene (BαP) on liver, gill, and blood of Caspian White fish. To this end, 150 fingerling fish (6.5 ± 0.8 g) were exposed to under, near and over environmental concentrations of BαP (i.e. 50, 100, and 200 ppb, respectively) and two control groups for 21 days. Following exposure to BαP, generally, DNA damage increased in the liver and gill cells as well as the frequency of micro- and bi-nucleated erythrocytes in a time and concentration-dependent pattern. In addition, the liver and gill tissues displayed several histopathological lesions. Together, the findings are warning the health status of the Caspian Sea due to an ever-increasing concentration of BαP through using Caspian White fish as an ecological model.

Effect of gamma radiation on the growth, survival, hematology and histological parameters of rainbow trout (Oncorhynchus mykiss) larvae.

Effects of low (1, 2.5 and 5Gy) and high doses (10, 20 and 40Gy) of gamma radiation were examined on the growth, survival, blood parameters and morphological changes of the intestines of rainbow trout (Oncorhynchus mykiss) larvae (103±20mg) after 12 weeks of exposure. Negative effects of gamma radiation on growth and survival were observed as radiation level and time increased. Changes were well documented at 10 and 20Gy. All the fish were dead at the dose of 40Gy. In all the treatments, levels of red blood cells (RBC), hematocrit (HCT) and hemoglobin (HB) were significantly (P<0.05) declined as the irradiation levels increased, whereas the amount of mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCH) did not change. No significant differences (P>0.05) were found in the levels of white blood cells (WBC), lymphocytes and monocytes. Destruction of the intestinal epithelium cells was indicated as the irradiation levels increased to 1Gy and above. The highest levels of growth, survival, specific growth rate (SGR), condition factor (CF) and protein efficiency rate (PER) were obtained in the control treatment. The results showed that gamma rays can be a potential means for damaging rainbow trout cells.