RESUMO
A CMOS fabricated silicon microchip was used as a platform for immunoassays and DNA synthesis and hybridization. The chip is covered with a biofriendly matrix wherein the chemistries occur. The active silicon chip has over 1000 active electrodes that can be individually addressed for both synthesis of DNA and protein attachment to a membrane on the chip surface. Additionally, the active chip can be further used for the detection of various analytes at the chip surface via digital read out resulting from the redox enzymes on the captured oligonucleotide or antibody.
Assuntos
DNA/análise , DNA/genética , Peroxidase do Rábano Silvestre/metabolismo , Imunoensaio/métodos , Análise de Sequência com Séries de Oligonucleotídeos/métodos , Bacteriófagos/imunologia , Bacteriófagos/isolamento & purificação , Eletroquímica , Eletrodos , Humanos , Imunoensaio/instrumentação , Luz , Análise de Sequência com Séries de Oligonucleotídeos/instrumentação , Orosomucoide/análise , Orosomucoide/imunologia , SoluçõesRESUMO
Protein arrays will greatly accelerate research and development in medical and biological sciences. We have used cell-free protein biosynthesis and a parallel immobilization strategy for producing protein biochips. We demonstrate a model two-protein microarray using luciferase and green fluorescent protein, both expressed in a cell-free system and specifically immobilized on CombiMatrix semiconductor oligonucleotide microarrays. This demonstration provides evidence for the appropriate folding, activity, robust presentation, and efficient flexible detection of proteins on the microscale.