Wake-Mediated Propulsion of an Upstream Particle in Two-Dimensional Plasma Crystals.

The wake-mediated propulsion of an "extra" particle in a channel of two neighboring rows of a two-dimensional plasma crystal, observed experimentally by Du et al. [Phys. Rev. E 89, 021101(R) (2014)PRESCM1539-375510.1103/PhysRevE.89.021101], is explained in simulations and theory. We use the simple model of a pointlike ion wake charge to reproduce this intriguing effect in simulations, allowing for a detailed investigation and a deeper understanding of the underlying dynamics. We show that the nonreciprocity of the particle interaction, owing to the wake charges, is responsible for a broken symmetry of the channel that enables a persistent self-propelled motion of the extra particle. We find good agreement of the terminal extra-particle velocity with our theoretical considerations and with experiments.

Assessing particle kinematics via template matching algorithms.

Template matching algorithms represent a viable tool to locate particles in optical images. A crucial factor of the performance of these methods is the choice of the similarity measure. Recently, it was shown in [Gao and Helgeson, Opt. Express 22 (2014)] that the correlation coefficient (CC) leads to good results. Here, we introduce the mutual information (MI) as a nonlinear similarity measure and compare the performance of the MI and the CC for different noise scenarios. It turns out that the mutual information leads to superior results in the case of signal dependent noise. We propose a novel approach to estimate the velocity of particles which is applicable in imaging scenarios where the particles appear elongated due to their movement. By designing a bank of anisotropic templates supposed to fit the elongation of the particles we are able to reliably estimate their velocity and direction of motion out of a single image.

Mode-coupling instability in a fluid two-dimensional complex plasma.

A theory of the mode-coupling instability (MCI) in a fluid two-dimensional complex plasma is developed. In analogy to the point-wake model of the wake-mediated interactions commonly used to describe MCI in two-dimensional crystals, the layer-wake model is employed for fluids. It is demonstrated that the wake-induced coupling of wave modes occurs in both crystalline and fluid complex plasmas, but the confinement-density threshold, which determines the MCI onset in crystals, virtually disappears in fluids. The theory shows excellent qualitative agreement with available experiments and provides certain predictions to be verified.

Nonthermal plasma affects viability and morphology of Mycoplasma hominis and Acholeplasma laidlawii.

AIM: To study the effects exerted by argon microwave nonthermal plasma (NTP) on cell wall-lacking Mollicutes bacteria. METHODS AND RESULTS: 10(8) CFU ml(-1) agar plated Mycoplasma hominis and Acholeplasma laidlawii were treated with the nonthermal microwave argon plasma for 30-300 s. The maximal 10- and 100-fold drop was observed for A. laidlawii and Myc. hominis, respectively. Similarly treated Escherichia coli and Staphylococcus aureus demonstrated the 10(5) and 10(3) drop, respectively. Removal of cholesterol affected resistance of A. laidlawii. 10 mmol l(-1) antioxidant butylated hydroxytoluene decreased mortality by a factor of 25-200. UV radiation alone caused 25-85% mortality in comparison with the whole NTP. Exogenously added hydrogen peroxide H2O2 did not cause mortality. NTP treatment of Myc. hominis triggered growth of microcolonies, which were several tenfold smaller than a typical colony. CONCLUSIONS: Despite the lack of cell wall, A. laidlawii and Myc. hominis were more resistant to argon microwave NTP than other tested bacteria. Mycoplasma hominis formed microcolonies upon NTP treatment. A role of UV and active species was demonstrated. SIGNIFICANCE AND IMPACT OF THE STUDY: The first study of NTP effects on Mollicutes revealed importance of a membrane composition for bacterial resistance to NTP. New specific Myc. hominis morphological forms were observed. The study confirmed importance of the concerted action of reactive oxygen species (ROS) with UV and other plasma bioactive agents for NTP bactericidal action.

Scale-free behavior of a 2D complex plasma during rapid cooling.

Experimental evidence is presented for a scale-free transition from an unordered to an ordered state in a 2D complex plasma that differs from the KTHNY theory of phase transitions in 2D systems. The transition is characterized by the formation and growth of ordered domains. A fractal relationship is found between the domain areas and domain boundary lengths, which can be explained by a recent theoretical model. The experimental findings are supported by a molecular dynamics simulation of a 2D particle system.

Investigation of the mutagenic potential of cold atmospheric plasma at bactericidal dosages.

In the past few years, cold atmospheric plasma (CAP) has evolved into a new tool in the fight against nosocomial infections and antibiotic-resistant microorganisms. The products generated by the plasma-electrons, ions, reactive species and UV light-represent a 'lethal cocktail' for different kinds of pathogen, which opens up possible applications in hygiene and medicine. Nevertheless, to ensure the safe usage of CAP on skin (e.g., to treat wounds or skin diseases) several pre-clinical in vitro studies have to be performed before implementing clinical trials on humans. In the study presented here, inactivation experiments with Escherichia coli were carried out to identify the necessary plasma dosage for a 5 log reduction: with a small hand-held battery-operated CAP device, these disinfection properties were achieved after application during 30s. This and higher plasma dosages were then used to analyze the mutagenicity induced in V79 Chinese hamster cells-to furthermore define a 'safe application window'-with the HPRT (hypoxanthine-guanine phosphoribosyl transferase) mutation assay. The results show that a CAP treatment of up to 240 s and repeated treatments of 30s every 12h did not induce mutagenicity at the Hprt locus beyond naturally occurring spontaneous mutations.

A randomized two-sided placebo-controlled study on the efficacy and safety of atmospheric non-thermal argon plasma for pruritus.

BACKGROUND: To look into new potential indications for physical plasma and because some reports suggest plasma having antipruritic effects, we investigated the treatment of pruritus that often represents a therapeutic challenge. OBJECTIVES: To assess the efficacy and safety of cold atmospheric argon plasma as add-on-therapy in pruritic diseases. METHODS: We treated 46 patients with various pruritic diseases with cold plasma for 2 min daily in addition to standard treatment. All patients served as their own control, when their pruritic disease was treated with argon gas (placebo). The outcome measure was a long-term and short-term reduction in itching measured by means of a visual analogue score (VAS). RESULTS: The VAS scores at baseline were comparable (plasma 4.57, SD 2.38, argon 4.34, SD 2.35). We did not find any significant differences in VAS reduction between plasma and argon: long-term VAS difference of 1.97 (SD 1.33) for plasma and 1.74 (SD 2.37) for argon [P = 0.224, 95% CI: (-0.15; 0.60)], short-term VAS difference of 1.92 (SD 1.33) for plasma and 1.97 (SD 1.29) for argon [P = 0.544, 95% CI: (-0.21; 0.11)]. In both groups, patients experienced a significant reduction of pruritus at the end of therapy compared to baseline [plasma 1.97 (P < 0.0001), placebo 1.74 [P < 0.0001)]. No relevant side effects occurred, and treatment was well tolerated. CONCLUSIONS: Treatment with cold plasma did not result in higher pruritus reduction than treatment with placebo. A significant reduction of pruritus compared to no effect was found at the end of therapy in both groups. Both treatment options had similar safety profiles.

Microstructure of a liquid two-dimensional dusty plasma under shear.

The microstructure of a strongly coupled liquid undergoing a shear flow was studied experimentally. The liquid was a shear melted two-dimensional plasma crystal, i.e., a single-layer suspension of micrometer-size particles in a rf discharge plasma. Trajectories of particles were measured using video microscopy. The resulting microstructure was anisotropic, with compressional and extensional axes at around ±45° to the flow direction. Corresponding ellipticity of the pair correlation function g(r) or static structure factor S(k) gives the (normalized) shear rate of the flow.

Three-dimensional structure of Mach cones in monolayer complex plasma crystals.

The structure of Mach cones in a crystalline complex plasma has been studied experimentally using an intensity sensitive imaging, which resolved particle motion in three dimensions. This revealed a previously unknown out-of-plane cone structure, which appeared due to excitation of the vertical wave mode. The complex plasma consisted of micron sized particles forming a monolayer in a plasma sheath of a gas discharge. Fast particles, spontaneously moving under the monolayer, created Mach cones with multiple structures. The in-plane cone structure was due to compressional and shear lattice waves.

Successful and safe use of 2 min cold atmospheric argon plasma in chronic wounds: results of a randomized controlled trial.

BACKGROUND: The development of antibiotic resistance by microorganisms is an increasing problem in medicine. In chronic wounds, bacterial colonization is associated with impaired healing. Cold atmospheric plasma is an innovative promising tool to deal with these problems. OBJECTIVES: The 5-min argon plasma treatment has already demonstrated efficacy in reducing bacterial numbers in chronic infected wounds in vivo. In this study we investigated a 2-min plasma treatment with the same device and the next-generation device, to assess safety and reduction in bacterial load, regardless of the kind of bacteria and their resistance level in chronic wounds. METHODS: Twenty-four patients with chronic infected wounds were treated in a prospective randomized controlled phase II study with 2 min of cold atmospheric argon plasma every day: 14 with MicroPlaSter alpha device, 10 with MicroPlaSter beta device (next-generation device) in addition to standard wound care. The patient acted as his/her own control. Bacterial species were detected by standard bacterial swabs and bacterial load by semiquantitative count on nitrocellulose filters. The plasma settings were the same as in the previous phase II study in which wounds were exposed for 5 min to argon plasma. RESULTS: Analysis of 70 treatments in 14 patients with the MicroPlaSter alpha device revealed a significant (40%, P<0.016) reduction in bacterial load in plasma-treated wounds, regardless of the species of bacteria. Analysis of 137 treatments in 10 patients with the MicroPlaSter beta device showed a highly significant reduction (23.5%, P<0.008) in bacterial load. No side-effects occurred and the treatment was well tolerated. CONCLUSIONS: A 2-min treatment with either of two cold atmospheric argon plasma devices is a safe, painless and effective technique to decrease the bacterial load in chronic wounds.

[New approaches to therapy of persistent infections: elimination of intracellular Chlamydai trachomatis by exposure to low temperature argon plasma].

AIM: Study microbicidal activity of low temperature argon plasma (LTP) that is a stream of partially ionized argon having macroscopic temperature of the environment against Chlamydia trachomatis obligate intracellular parasites. Study viability of host cells in parallel. MATERIALS AND METHODS: McCoy line cells infected with C. trachomatis (Bu-434/L2 strain) were exposed to LTP obtained by using atmospheric pressure plasma SHF generator. Intracellular localization of chlamydiae was visualized by luminescent microscopy. RESULTS: Exposure of infected McCoy line cells resulted in the destruction of chlamydia inclusions and practically complete elimination of intracellular bacteria. At the same time LTP exposure did not result in immediate death of host cells, an insignificant reduction of the number of cells was observed 24 hours after the exposure to LTP. CONCLUSION: The effect of LTP for elimination of intracellular chlamydia without significant changes in viability of eukaryotic host cells was demonstrated.

In situ characterization of nanoparticles during growth by means of white light scattering.

A simple method of characterization of suspensions of spherical nanoparticles with monotonically variable size is proposed. It allows for the in situ measurement of the particle size as well as spectral dependence of their refractive indices. The method requires three optical channels: one for the illumination of a suspension by white light and two for the measurements of the spectra of scattered light. Parameters of the particles are determined by fitting the measured temporal spectral surfaces by the calculated Mie scattering functions. The method is applied to the particles being grown in a low-pressure reactive plasma of a discharge in an acetylene-argon mixture.

Direct experimental measurement of the speed-stress relation for dislocations in a plasma crystal.

The speed-stress relation for gliding edge dislocations was experimentally measured for the first time. The experimental system used, a two-dimensional plasma crystal, allowed observation of individual dislocations at the "atomistic" level and in real time. At low applied stress dislocations moved subsonically, at higher stress their speed abruptly increased to 1.9 times the speed of shear waves, then slowly grew with stress. There is evidence that immediately after nucleation dislocations can move faster than pressure waves.

Pattern formation in a complex plasma in high magnetic fields.

Low-pressure room-temperature neon, argon, krypton, and air plasmas were studied in magnetic fields up to flux densities of 2.3 T. Filaments appeared parallel to the magnetic field lines, and patterns such as spirals and concentric circles formed in the perpendicular direction. We link these effects to the magnetization of the ions. We also used a layer of embedded microparticles as probes in the plasma. Their motion changed dramatically from a collective rotation of the whole ensemble in moderate magnetic fields to a rotation in several small vortices centered at the filaments.

Complex plasmas in external fields: the role of non-hamiltonian interactions.

Dedicated experiments with strongly coupled complex plasmas in external electric fields were carried out under microgravity conditions using the PK-4 dc discharge setup. The focus was put on the comparative analysis of the formation of stringlike anisotropic structures due to reciprocal (hamiltonian) and nonreciprocal (non-hamiltonian) interactions between microparticles (induced by ac and dc fields, respectively). The experiments complemented by numerical simulations demonstrate that the responses of complex plasmas in these two regimes are drastically different. It is suggested that the observed difference is a manifestation of intrinsic thermodynamic openness of driven strongly coupled systems.

Freezing and melting of 3D complex plasma structures under microgravity conditions driven by neutral gas pressure manipulation.

Freezing and melting of large three-dimensional complex plasmas under microgravity conditions is investigated. The neutral gas pressure is used as a control parameter to trigger the phase changes: Complex plasma freezes (melts) by decreasing (increasing) the pressure. The evolution of complex plasma structural properties upon pressure variation is studied. Theoretical estimates allow us to identify the main factors responsible for the observed behavior.

Plasma applications in medicine with a special focus on dermatology.

The recent tremendous progress in understanding physical plasma phenomenon, together with the development of new plasma sources has put growing focus on the application of plasmas in health care. Active plasma components, such as molecules, atoms, ions, electrons and photons, reactive species, ultraviolet radiation, optical and infrared emission and heat have the ability of activating, controlling and catalysing reactions and complex biochemical procedures. Thermal and non-thermal (i.e. cold) plasmas - both already widely established in medicine - are used for various therapeutic applications. Particularly in dermatology, plasma applications hold big potential, for example, in wound healing, such as efficient disinfection or sterilization, therapy of various skin infections or tissue regeneration. This review gives an overview on potential plasma applications in medicine - including the recent research on skin diseases - and summarizes possible interactions between plasmas and living tissue.

[Prospects for the use of low-temperature gas plasma as an antimicrobial agent].

Results of application of LTP at atmospheric pressure as an antibacterial agent during the last decade are considered with reference to physicochemical mechanisms of its bactericidal action. The principles of designing modern LTP sources are described in conjunction with the results of LTP application against pathogenic bacteria in vitro and in biofilms. The possibility to destroy biofilm matrix by LTP is estimated along with the results of its testing for the treatment of acute and chronic wound surfaces. Prospects for the development of "plasma medicine" in this country and abroad are discussed with special emphasis on its advantages, such as the absence of long-acting toxic compounds, small probability of spontaneous mutations accounting for resistance to LTP, relatively low cost of LTP sources, independence of LTP effect of the surface relief, painless application.

Direct observation of mode-coupling instability in two-dimensional plasma crystals.

Dedicated experiments on melting of two-dimensional plasma crystals were carried out. The melting was always accompanied by spontaneous growth of the particle kinetic energy, suggesting a universal plasma-driven mechanism underlying the process. By measuring three principal dust-lattice wave modes simultaneously, it is unambiguously demonstrated that the melting occurs due to the resonance coupling between two of the dust-lattice modes. The variation of the wave modes with the experimental conditions, including the emergence of the resonant (hybrid) branch, reveals exceptionally good agreement with the theory of mode-coupling instability.

Kinetics of fluid demixing in complex plasmas: role of two-scale interactions.

Using experiments and combining theory and computer simulations, we show that binary complex plasmas are particularly good model systems to study the kinetics of fluid-fluid demixing at the "atomistic" (individual particle) level. The essential parameters of interparticle interactions in complex plasmas, such as the interaction range(s) and degree of nonadditivity, can be varied significantly, which allows systematic investigations of different demixing regimes. The critical role of competition between long-range and short-range interactions at the initial stage of the spinodal decomposition is discussed.