No city for wetland species: habitat associations affect mammal persistence in urban areas.

The fast rate of replacement of natural areas by expanding cities is a key threat to wildlife worldwide. Many wild species occur in cities, yet little is known on the dynamics of urban wildlife assemblages due to species' extinction and colonization that may occur in response to the rapidly evolving conditions within urban areas. Namely, species' ability to spread within urban areas, besides habitat preferences, is likely to shape the fate of species once they occur in a city. Here we use a long-term dataset on mammals occurring in one of the largest and most ancient cities in Europe to assess whether and how spatial spread and association with specific habitats drive the probability of local extinction within cities. Our analysis included mammalian records dating between years 1832 and 2023, and revealed that local extinctions in urban areas are biased towards species associated with wetlands and that were naturally rare within the city. Besides highlighting the role of wetlands within urban areas for conserving wildlife, our work also highlights the importance of long-term biodiversity monitoring in highly dynamic habitats such as cities, as a key asset to better understand wildlife trends and thus foster more sustainable and biodiversity-friendly cities.

Public perception and acceptance of coypu Myocastor coypus removal in urban areas: influences of age and education.

Monitoring and management of alien coypu (Myocastor coypus) is a key issue in Europe since this species has been included in the EU Invasive Alien Species Regulation 1143/2014. Thus, controlling the population of this rodent is considered as imperative by wildlife managers. Coypu management in urban areas is crucial considering potential conflicts with human activities. The aim of this study is to investigate citizens' knowledge, perceptions and opinions towards the presence and management of coypu in a case study in Central Italy (the Serravalle urban park, Tuscany). The survey was administered to a subset of municipal residents and garnered responses from 281 park visitors. The outcomes showed a high level of knowledge of respondents: 99.3% of total respondents had heard of coypu before this survey, and 93.9% were able to distinguish the coypu from the Eurasian beaver (Castor fiber). Despite the importance of coypu control in invaded countries, our results highlight that most respondents are against the removal of coypu, with the exception of most young, highly-educated and upperclass men, but favouring sterilization over capture and population control. Findings of this study could assist managers in enhancing the efficacy of management efforts via an information initiative involving the general public. Achieving this goal may entail improved communication by wildlife managers, which is imperative for optimizing management strategies regarding alien species.

Different facets of the same niche: Integrating citizen science and scientific survey data to predict biological invasion risk under multiple global change drivers.

Citizen science initiatives have been increasingly used by researchers as a source of occurrence data to model the distribution of alien species. Since citizen science presence-only data suffer from some fundamental issues, efforts have been made to combine these data with those provided by scientifically structured surveys. Surprisingly, only a few studies proposing data integration evaluated the contribution of this process to the effective sampling of species' environmental niches and, consequently, its effect on model predictions on new time intervals. We relied on niche overlap analyses, machine learning classification algorithms and ecological niche models to compare the ability of data from citizen science and scientific surveys, along with their integration, in capturing the realized niche of 13 invasive alien species in Italy. Moreover, we assessed differences in current and future invasion risk predicted by each data set under multiple global change scenarios. We showed that data from citizen science and scientific surveys captured similar species niches though highlighting exclusive portions associated with clearly identifiable environmental conditions. In terrestrial species, citizen science data granted the highest gain in environmental space to the pooled niches, determining an increased future biological invasion risk. A few aquatic species modelled at the regional scale reported a net loss in the pooled niches compared to their scientific survey niches, suggesting that citizen science data may also lead to contraction in pooled niches. For these species, models predicted a lower future biological invasion risk. These findings indicate that citizen science data may represent a valuable contribution to predicting future spread of invasive alien species, especially within national-scale programmes. At the same time, citizen science data collected on species poorly known to citizen scientists, or in strictly local contexts, may strongly affect the niche quantification of these taxa and the prediction of their future biological invasion risk.

Eurasian beavers in Central Italy: perceptions in the local community.

According to the IUCN guidelines, wildlife reintroduction should consider any impacts on humans within feasibility assessments. Eurasian beavers Castor fiber are recovering across their native range, due to protection laws and reintroductions. In Central Italy, a self-sustaining, naturalised population of Eurasian beavers has been identified in the last five years. A questionnaire to measure whether and how citizens in the local area perceive the presence of the beaver was administered to 1114 respondents. We observed a comprehensive awareness of the presence of the beaver in Italy and a high ability to distinguish it from non-native coypus Myocastor coypus (92.3%). We also recorded a general high knowledge of issues related to the presence of the beaver (i.e., potential effects on indigenous biodiversity). The majority (65.5%) of the surveyed population was in favour of reintroducing the beaver in Central Italy, and only 1.2% was firmly against it. The majority of interviewed people was against the removal of beavers from Central Italy (65.8%), whereas only 3.7% was in favor, citing fears of perceived impacts on the river, crops, and fish populations.

Spatial ecology of crested porcupine in a metropolitan landscape.

Human settlements, including cities, may provide wildlife with new ecological niches, in terms of habitat types and food availability, thus requiring plasticity for adaptation. The crested porcupine Hystrix cristata is a habitat-generalist, large-sized rodent, also recorded in some suburban areas, but no information is available on its habitat use in metropolitan landscapes. Here, we assessed the land-use factors influencing the presence of crested porcupines in a metropolitan area of Central Italy. We collected data on the occurrence of crested porcupines from the metropolitan area of Rome, following an observer-oriented approach to record occurrences and retreive pseudo-absences. We then related the presence/absence of H. cristata to landscape composition. Occupancy models showed that cultivations and scrubland were positively related to porcupine presence, most likely as they provide food resources and shelter sites, respectively. Although the crested porcupine has been confirmed as a "generalist" species in terms of habitat selection, a strong preference for areas limiting the risk of being killed and providing enough food and shelter was observed. We therefore suggest that the crested porcupine may adapt to deeply modified landscapes such as large cities by selecting specific favourable land-use types.

The good, the bad and the ugly of COVID-19 lockdown effects on wildlife conservation: Insights from the first European locked down country.

The COVID-19 pandemic zoonosis has determined extensive lockdowns worldwide that provide an unprecedented opportunity to understand how large-scale shifts of human activities can impact wildlife. We addressed the impacts of the COVID-19 lockdown on wildlife in Italy, the first European country that performed a countrywide lockdown, and identified potentially beneficial and negative consequences for wildlife conservation and management. We combined a qualitative analysis of social media information with field data from multiple taxa, data from citizen science projects, and questionnaires addressed to managers of protected areas. Both social media information and field data suggest that a reduction of human disturbance allowed wildlife to exploit new habitats and increase daily activity. The field data confirmed some positive effects on wildlife conservation, such as an increase in species richness in temporarily less-disturbed habitats, a higher breeding success of an aerial insectivorous bird, and reduction of road-killing of both amphibians and reptiles. Despite some positive effects, our data also highlighted several negative impacts of the COVID-19 crisis on wildlife. The lower human disturbance linked to lockdown was in fact beneficial for invasive alien species. Results from questionnaires addressed to managers of protected areas highlighted that the COVID-19 lockdown interrupted actions for the control of invasive alien species, and hampered conservation activities targeting threatened taxa. Furthermore, the reduction of enforcement could cause a surge of illegal killing of wildlife. The COVID-19 crisis, besides having deep socio-economic impacts, might profoundly affect wildlife conservation, with potentially long-lasting effects.

Assessment of color response and activity rhythms of the invasive black planthopper Ricania speculum (Walker, 1851) using sticky traps.

To be effective, management strategies of invasive alien species cannot ignore their spatiotemporal behavior particularly those exerting serious damages to human activities. The black planthopper Ricania speculum is an Asian insect that has been reported as an alien invasive species in Italy, where it threatens local plant diversity, including important crops. In our work, we analyzed the activity rhythms of this species through circular statistics and the efficiency of chromotropic traps to capture adult individuals. Captures were carried out in central Italy, where the black planthopper is showing a remarkable range expansion, after its first discovery in 2009. We observed that the species was mainly crepuscular, with a high intersexual activity overlap. Activity rhythms changed between July-August and September-October, with changing heliophany, but peaked at sunset and were the lowest in the second half of the night and early morning. The insects were mostly caught by green traps, particularly in September, which is the period of egg-laying inside the leaves; conversely, orange ones were avoided, and yellow ones captured proportionally to their local availability. Strategies for controlling this species should consider concentrating trapping effort during the activity peak, using green sticky traps to enhance the capture success of each trap, with the lowest impact over non-target species.

Patterns of activity rhythms of invasive coypus Myocastor coypus inferred through camera-trapping.

Studies on activity rhythms are pivotal for the management of invasive alien species, as they provide basic insights into species basic ecology and may increase the success of control programs. The coypu Myocastor coypus, introduced from South America for fur farms, has become one of the most invasive rodents in Europe. Introduced coypus may affect crop productions, as well as natural vegetation and the breeding success of wading birds. In this study, we examined activity data collected through intensive camera-trapping in three Italian areas, including two natural areas in Northern and Central Italy, and a suburban area in Central Italy. Coypus were mostly diurnal in areas characterised by low predator pressure and, at night, they are mostly active in bright moonlight. Conversely, where predators, human pressure or numerical control programmes are present, coypus remarkably shift their behaviour towards crepuscular and night hours. In these last areas, nocturnal activity increased as moonlight decreased, possibly to reduce predation risk or encounters with humans. Where winter temperature are low, diurnal habits may have developed as a physiological adaptation and a strategy to preserve energy, potentially achieving a cost/effective thermal balance.

Arthropods and associated pathogens from native and introduced rodents in Northeastern Italy.

Introduced Siberian chipmunks Eutamias sibiricus have been reported to be important reservoirs for human Lyme disease, as they may host high numbers of hard ticks carrying Borrelia spp. and other pathogens. In the present study, we assessed the prevalence of Borrelia spp. and other pathogenic bacteria in ectoparasite arthropod species infesting Siberian chipmunks and coexisting native small rodents. Small rodents were trapped with Sherman traps in Veneto (NE Italy), where the largest Italian populations of chipmunks occur. A total of 14 individual ticks were found on 223 rodents, with 6 more ticks obtained from stored dead chipmunks from the same study area. Ectoparasites were screened for pathogens by molecular analyses including species-specific PCR amplifications. Rickettsia monacensis, Borrelia lusitaniae, and Anaplasma platys were present in the parasites of both native rodents and introduced chipmunks. The present findings suggest a role for the invasive species E. sibiricus in the maintenance of the Ixodes ricinus life cycle, which may result in the modification of the transmission dynamics of tick-borne pathogens. Moreover, the presence of Rickettsia in urban populations of chipmunks may represent a serious risk for human health and should be investigated further.

Assessment of Detection Methods and Vegetation Associations for Introduced Finlayson's Squirrels (Callosciurus finlaysonii) in Italy.

Managing biological invasions requires rapid, cost-effective assessments of introduced species' occurrence, and a good understanding of the species' vegetation associations. This is particularly true for species that are elusive or may spread rapidly. Finlayson's squirrel (Callosciurus finlaysonii) is native to Thailand and southeastern Asia, and two introduced populations occur in peninsular Italy. One of the two introduced populations is rapidly expanding, but neither effective monitoring protocols nor reliable information on vegetation associations are available. To fill this gap, we conducted visual surveys and hair tube sampling in a periurban landscape of southern Italy to compare the effectiveness of these two methods in assessing presence of Finlayson's squirrel. We also determined the species' association with vegetation types at detection locations and nesting sites. Both visual and hair tube sampling effectively assessed the species' presence, but hair tubes resulted in fewer false absences. Moreover, when we controlled for the costs of labor and equipment, hair tubes were 33.1% less expensive than visual sampling. Presence of squirrels and their nests was positively correlated with shrub species richness, indicating that the occurrence of forests with well-developed understory may inhibit the spread of the species.

Long live the alien: is high genetic diversity a pivotal aspect of crested porcupine (Hystrix cristata) long-lasting and successful invasion?

Studying the evolutionary dynamics of an alien species surviving and continuing to expand after several generations can provide fundamental information on the relevant features of clearly successful invasions. Here, we tackle this task by investigating the dynamics of the genetic diversity in invasive crested porcupine (Hystrix cristata) populations, introduced to Italy about 1500 years ago, which are still growing in size, distribution range and ecological niche. Using genome-wide RAD markers, we describe the structure of the genetic diversity and the demographic dynamics of the H. cristata invasive populations and compare their genetic diversity with that of native African populations of both H. cristata and its sister species, H. africaeaustralis. First, we demonstrate that genetic diversity is lower in both the invasive Italian and the North Africa source range relative to other native populations from sub-Saharan and South Africa. Second, we find evidence of multiple introduction events in the invasive range followed by very limited gene flow. Through coalescence-based demographic reconstructions, we also show that the bottleneck at introduction was mild and did not affect the introduced genetic diversity. Finally, we reveal that the current spatial expansion at the northern boundary of the range is following a leading-edge model characterized by a general reduction of genetic diversity towards the edge of the expanding range. We conclude that the level of genome-wide diversity of H. cristata invasive populations is less important in explaining its successful invasion than species-specific life-history traits or the phylogeographic history in the native source range.

Oscheius tipulae in Italy: Evidence of an Alien Isolate in the Integral Natural Reserve of Montecristo Island (Tuscany).

Montecristo Island is an integral natural reserve of the Tuscan Archipelago National Park (Central Italy), characterized by a peculiar assemblage of flora and fauna, with several endemic taxa, and also with a high number of alien species. During a soil survey, we found an alien Oscheius tipulae Lam & Webster, 1971 isolate, phylogenetically close to others from South America. In this article, we examined the possible pathways of introduction of this nematode. Because of the high number of alien plants in this protected area and the low desiccation survival ability of O. tipulae, we hypothesized that the presence of this alien nematode isolate may be related to the soil of introduced plants, although historical association with plant-associated invertebrates is also possible. Further studies with more populations and marker molecules are necessary to investigate the distribution of O. tipulae and the possible impact on this natural reserve.

Ectoparasite load in the crested porcupine Hystrix cristata Linnaeus, 1758 in Central Italy.

The crested porcupine Hystrix cristata is a large body-sized rodent, occurring in Europe only in the Italian Peninsula, where it may have been introduced in early Medieval times. Its parasite fauna is currently poorly known and limited to few anecdotal observations. We have analyzed the ectoparasite load of 165 crested porcupines from Tuscany and Latium (Central Italy). Both captured and road-killed individuals were checked for fleas and ticks. Overall, only 39 porcupines were infested by four species of ticks and five of fleas. Abundance of ectoparasites was higher in areas with higher habitat richness, with respect to densely wooded areas. The most frequent species was the flea Pulex irritans (25%), whose prevalence peaked in winter probably because of optimal abiotic conditions in the porcupine's den. The remaining species of both hard ticks (Rhipicephalus bursa, Pholeoixodes hexagonus, and Ixodes ventalloi) and fleas (Paraceras melis, Ctenocephalides canis, Dasypsyllus gallinulae, and Hystrichopsylla talpae), all with prevalence lower than 5%, could be due to den sharing with other vertebrates, mainly carnivores such as, e.g., red foxes and badgers. The second most prevalent species was the generalist tick Ixodes ricinus (21%). An adult male-biased parasitism for ticks has been detected, suggesting a possible role of testosterone related immune-depressive effect. The low richness in dominant ectoparasite species, built up by locally acquired generalist taxa, provides support to the allochthonous origin of this rodent in Italy.

Macroparasites of introduced parakeets in Italy: a possible role for parasite-mediated competition.

Alien species are considered a cause of biodiversity loss throughout the world. An important but often overlooked form of competition with native species is the parasite-mediated one. Introduced species may bring their own parasites from their native ranges (spillover) or get native parasites from native species, thus increasing the parasites' spread and transmission risk (spillback). Thus, a complete knowledge of parasites hosted by introduced species is important to assess and to possibly prevent impacts. Ring-necked and monk parakeets have been introduced in many European countries, where they established a number of alien reproductive populations. We sampled 21 ring-necked parakeets and 7 monk parakeets from Italy and identified 35 arthropod ectoparasites belonging to five species. Amongst those, one species was native to India (Neopsittaconirmus lybartota), where alien populations of ring-necked parakeet may have been originated, and one species from South America (Paragoniocotes fulvofasciatus), which is typically found of the monk parakeet in its native range. The other three species of arthropod parasites were native to Italy and commonly found on native species, suggesting the possibility of spillback processes.

Host specificity of the badger's flea (Paraceras melis) and first detection on a bat host.

Defining the whole spectrum of potential hosts of a parasite has large epidemiological and evolutionary implications in biology. Specialized parasites might be able to occasionally exploit a range of different host species, increasing the individual survival and the chances of successful dispersal. For long time Paraceras melis has been considered a specific flea of European badger Meles meles. Anyway, it has occasionally been reported on different hosts. In this work, we summarize the host spectrum of P. melis from literature and we report its first detection on a bat host. Ten species were identified as occasional hosts, man included, and the plasticity of this flea in host exploitation is noteworthy because of possible increase of pathogens transmission to humans and domestic species.

A forensic genetic investigation reveals a captive origin for a wild alien population of raccoons in Italy.

Invasive alien species have extensively impacted the ecosystems, where they may affect the native biodiversity. The North American raccoon Procyon lotor is one of the most successful invaders in Europe since its introduction in the early twentieth century. In Italy, a wild population was first established in the North at the beginning of the 2000s following a local introduction event. A further self-sustaining population was reported ten years later in Central Italy. To support an official investigation by the authorities, who suspected a captive origin of the free-ranging raccoons in Central Italy, we used nuclear and mitochondrial DNA markers, combined with different statistical approaches, to characterise their gene pool and trace the source of the founders. Results revealed that founders came from a private zoo-park from which they had inadvertently escaped, soon establishing a reproductive population in the wild. Additionally, our mitochondrial DNA data were used to supplement the haplotype variability known to date in captive and wild raccoons from Europe, Asia and their native range. The comparisons allowed us to update previous networks based on the control region with a new mitochondrial lineage, which had not been detected so far.

Aliens on the Road: Surveying Wildlife Roadkill to Assess the Risk of Biological Invasion.

Monitoring the presence and distribution of alien species is pivotal to assessing the risk of biological invasion. In our study, we carried out a worldwide review of roadkill data to investigate geographical patterns of biological invasions. We hypothesise that roadkill data from published literature can turn out to be a valuable resource for researchers and wildlife managers, especially when more focused surveys cannot be performed. We retrieved a total of 2314 works published until January 2022. Among those, only 41 (including our original data) fitted our requirements (i.e., including a total list of roadkilled terrestrial vertebrates, with a number of affected individuals for each species) and were included in our analysis. All roadkilled species from retrieved studies were classified as native or introduced (domestic, paleo-introduced, or recently released). We found that a higher number of introduced species would be recorded among roadkill in Mediterranean and Temperate areas with respect to Tropical and Desert biomes. This is definitely in line with the current knowledge on alien species distribution at the global scale, thus confirming that roadkill datasets can be used beyond the study of road impacts, such as for an assessment of different levels of biological invasions among different countries.

Razorbills Alca torda in Italian Seas: A Massive Irruption of Historical Relevance and Role of Social Network Monitoring.

Reporting on uncommon wide animal movements could help in depicting potential carry-over effects at the population level, particularly in an era of rapid climate and environmental changes. The razorbill (Alca torda, Linnaeus 1758) is a regular passage migrant and winter visitor to Italian seas, but with sporadic presences usually involving small numbers of individuals. Irruptions have been occasionally documented, with the last records of an unusually large number dating back to 1982. However, in the past, irruptions have only been locally reported and poorly described. Here we report on an unprecedented massive irruption of hundreds of razorbills which occurred in the central Mediterranean Sea in November-December 2022. Using citizen science platforms and photos/videos shared on social networking sites (SNSs), we estimated the relative magnitude of the irruption and described the spatial distribution of birds at sea, as well as report cases of stranded individuals. We collected a total of 267 records, both from Italy and from neighboring countries. We also discuss the likely factors affecting razorbill irruption and stress the importance of open social platforms and data sharing to aid in the early detection and estimation of such events at a wide-scale, as well as for the monitoring of the mortality of the irrupted species.

The magpie and the grapes: increasing ozone exposure impacts fruit consumption by a common corvid in a suburban environment.

BACKGROUND: The Eurasian magpie Pica pica is a resident bird species able to colonize farmlands and anthropized environments. This corvid shows a wide trophic spectrum by including fruits, invertebrates, small vertebrates and carcasses in its diet. A camera-trap experiment was carried out to test the effect of different ozone (O3 ) concentrations on potted Vitis vinifera plants, which resulted in different grape consumption rates by suburban birds. The test was performed at an Ozone-Free Air Controlled Exposure (FACE) facility, consisting of nine plots with three ozone (O3 ) levels: AA (ambient O3 concentration); and two elevated O3 levels, 1.5× AA (ambient air with a 50% increase in O3 concentration) and 2.0× AA (ambient air with a 100% increase in O3 concentration). Camera-traps were located in front of each treatment area and kept active for 24 h day-1 and for 5 days at a time over a period of 3 months to monitor grape consumption by birds. RESULTS: We collected a total of 38 videos. Eurasian magpies were the only grape consumers, with a total of 6.7 ± 3.3 passages per hour (mean ± SD) and no differences across the different O3 treatments. Grapes in the AA treatment were consumed significantly more quickly than those in the 1.5× AA treatment, which in turn, were consumed faster than those in the 2.0× AA treatment. At 3 days from the start of treatment, 94%, 53% and 22% berries from the AA, 1.5× AA and 2.0× AA treatments had been eaten, respectively. When the O3 was turned off, berries were consumed at the same rate among treatments. CONCLUSION: Increasing O3 concentrations limited grape consumption by magpies probably because O3 acted as a deterrent for magpies, although the lower sugar content recorded in the 2.0× AA berries did not affect the consumption when O3 was turned off. Our results provided valuable insights to mitigate human-wildlife conflicts in suburban environments. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

"Oops a Beaver Again!" Eurasian Beaver Castor fiber Recorded by Citizen-Science in New Areas of Central and Southern Italy.

The Eurasian beaver Castor fiber was once present in the Palearctic, ranging from the western Iberian Peninsula to northwestern China. In the Middle Ages, this rodent underwent a severe decline in population due to habitat loss, hunting for fur and meat, and the demand for castoreum. At the beginning of 1900, the range of the Eurasian beaver was limited to scattered refugia in Eurasia. Since 1920, legal protection, reintroduction events, and natural spread triggered the recovery of the species in most of its original range. In March 2021, the presence of the Eurasian beaver in Central Italy (Tuscany and Umbria regions) was confirmed through camera trapping after the detection of unequivocal signs of presence (i.e., gnawed trunks). Recordings are located about 550 km south of the known range of this species, thus suggesting that the presence of beavers in Tuscany and Umbria might be due to a local unauthorised reintroduction. In this work, we also reported the presence of beavers in the Abruzzi region and in Southern Italy (Molise-Campania regions), over 380 km in a straight line south to the southernmost record of beaver presence in Central Italy.