RESUMO
We propose a method to accelerate small-angle scattering experiments by exploiting spatial correlation in two-dimensional data. We applied kernel density estimation to the average of a hundred short scans and evaluated noise reduction effects of kernel density estimation (smoothing). Although there is no advantage of using smoothing for isotropic data due to the powerful noise reduction effect of radial averaging, smoothing with a statistically and physically appropriate kernel can shorten measurement time by less than half to obtain sector averages with comparable statistical quality to that of sector averages without smoothing. This benefit will encourage researchers not to use full radial average on anisotropic data sacrificing anisotropy for statistical quality. We also confirmed that statistically reasonable estimation of measurement time is feasible on site by evaluating how intensity variances improve with accumulating counts. The noise reduction effect of smoothing will bring benefits to a wide range of applications from efficient use of beamtime at laboratories and large experimental facilities to stroboscopic measurements suffering low statistical quality.
RESUMO
In some cell types, gap junctional intercellular communication (GJIC) is associated with tight junctions. The present study was performed to determine the roles of GJIC in regulation of the barrier function of tight junctions. Caco-2 human colonic cells were used as a monolayer model, and barrier function was monitored by measuring mannitol permeability and transepithelial electrical resistance (TER). The monolayers were chemically disrupted by treatment with oleic acid and taurocholic acid. Western blotting analyses were performed to evaluate the protein levels of connexins, which are components of gap junctional intercellular channels. Cx26 expression was detected in preconfluent Caco-2 cells, and its level increased gradually after the monolayer reached confluency. These results prompted us to examine whether overexpression of Cx26 affects barrier function. Monolayers of Caco-2 cells stably expressing Cx26 showed significantly lower mannitol permeability and higher TER than mock transfectants when the monolayers were chemically disrupted. The levels of claudin-4, an important component of tight junctions, were significantly increased in the stable Cx26 transfectant. These results suggest that Cx26-mediated GJIC may play a crucial role in enhancing the barrier function of Caco-2 cell monolayers.