Evidence of the functional role of the ethylene receptor genes SlETR4 and SlETR5 in ethylene signal transduction in tomato.

Ethylene receptors are key factors for ethylene signal transduction. In tomato, six ethylene receptor genes (SlETR1-SlETR6) have been identified. Mutations in different ethylene receptor genes result in different phenotypes that are useful for elucidating the roles of each gene. In this study, we screened mutants of two ethylene receptor genes, SLETR4 and SLETR5, from a Micro-Tom mutant library generated by TILLING. We identified two ethylene receptor mutants with altered phenotypes and named them Sletr4-1 and Sletr5-1. Sletr4-1 has a mutation between the transmembrane and GAF domains, while Sletr5-1 has a mutation within the GAF domain. Sletr4-1 showed increased hypocotyl and root lengths, compared to those of wild type plants, under ethylene exposure. Moreover, the fruit shelf life of this mutant was extended, titratable acidity was increased and total soluble solids were decreased, suggesting a reduced ethylene sensitivity. In contrast, in the absence of exogenous ethylene, the hypocotyl and root lengths of Sletr5-1 were shorter than those of the wild type, and the fruit shelf life was shorter, suggesting that these mutants have increased ethylene sensitivity. Gene expression analysis showed that SlNR was up-regulated in the Sletr5-1 mutant line, in contrast to the down-regulation observed in the Sletr4-1 mutant line, while the down-regulation of SlCTR1, SlEIN2, SlEIL1, SlEIL3, and SlERF.E4 was observed in Sletr4-1 mutant allele, suggesting that these two ethylene receptors have functional roles in ethylene signalling and demonstrating, for the first time, a function of the GAF domain of ethylene receptors. These results suggest that the Sletr4-1 and Sletr5-1 mutants are useful for elucidating the complex mechanisms of ethylene signalling through the analysis of ethylene receptors in tomato.

Enhancing betacyanin stability: Comparison of dragon fruit (Hylocereus polyrhizus) pulp and peel powders through encapsulation technology during storage.

Betacyanin can be found in the peel or pulp of dragon fruit. As a natural pigment, betacyanin is unstable, so it requires encapsulation technology to maintain its quality. The stability of encapsulated betacyanin from dragon fruit peel compared to dragon fruit pulp has yet to be discovered. This study aims to compare the stability of encapsulated betacyanin (with maltodextrin and gum Arabic) from dragon fruit peel and pulp dried with vacuum drying. Dragon fruit peel extraction utilized a 50% aqueous ethanol solvent, while pulp juice extraction was performed. The ratio of dragon fruit extract to coating materials was set at 1:3 (solid/solid). Research shows that dragon fruit juice powder had higher stability and phytochemical concentrations than the ethanol extract of dragon fruit peel powder during 30 days of storage. Despite similar color stability (similar range value of ΔE), the color from dragon fruit juice powder more closely resembled the natural fruit, albeit with weaker antioxidant activity than the peel powder. Betacyanin concentration in juice powder was notably higher (82.56-156.82 µg/g) than in the ethanol extract of dragon fruit peel powder (52.51-75.12 µg/g). A combination of maltodextrin and Arabic gum (1:1) as coating materials demonstrated the highest concentrations of total phenolic and total betacyanin (81.15-95.87 mg/g and 121.91-156.82 µg/g, respectively) during the storage period. These findings contribute to our comprehension of betacyanin stability and functionality, facilitating precise applications in industrial processing environments based on their source attributes.

Antioxidative response of parthenocarpic tomato, iaa9-3 and iaa9-5, under heat stress condition.

It has previously been shown that parthenocarpic tomato mutants, iaa9-3 and iaa9-5, can adapt, grow, and produce fruit under heat-stress conditions. However, the physiological processes in those two mutants especially for the enzymatic system that works to neutralize ROS are not clear. The objective of this research was to determine how the scavenging enzyme system responds to the heat stress in those mutants. The iaa9-3, iaa9-5, and WT-MT as a control were cultivated under two environmental conditions; normal and heat stress conditions. Vegetative and reproductive growth were observed during cultivation period. The activities of catalase (CAT), ascorbate peroxidase (APX) and superoxide dismutase (SOD) were investigated in both wild-type and parthenocarpic tomato mutants under normal and heat stress conditions. The results showed that under heat stress condition, the mutants, iaa9-3 and iaa9-5, and WT-MT resulted in reduction of the vegetative growth, but those mutants showed better growth than WT-MT. Higher chlorophyll content in iaa9-3 and iaa9-5 was observed under normal or heat stress condition. Despite their growth reduction under heat stress conditions, iaa9-3 and iaa9-5 resulted in the significant higher CAT, APX and SOD activity than WT-MT. The results suggest that higher chlorophyll content and enhanced CAT, APX and SOD activity in the iaa9-3 and iaa9-5 mutants are adaptive strategies to survive in heat stress conditions.

Vitamin C variation in citrus in response to genotypes, storage temperatures, and storage times: A systematic review and meta-analysis.

Numerous published studies have highlighted discrepancies in the duration and storage temperature used for preserving vitamin C content on various citrus genotypes worldwide. The present study aimed to analyze the variation in vitamin C content as influenced by citrus genotype, duration, and storage temperature using meta-analysis approaches. Data searching, selection, and tabulation resulted in a comprehensive database constructed from 1412 data points gathered from 54 individual studies, following PRISMA-P guidelines. The vitamin C content varied widely, ranging from 0 to 76.2 mg/100 mL in whole data of citrus fruit. Meta-analysis findings revealed that the duration of storage significantly impacted the vitamin C content in citrus fruits. Specifically, for grapefruit, mandarin, and orange, the length of storage significantly influenced their vitamin C levels (P < 0.01), with a consistent decrease observed over time. The correlation coefficients (R2) were 0.63 for grapefruit, 0.9 for mandarin, and 0.69 for orange. In contrast, no significant difference was found in terms of vitamin C levels between hybrid and lime citrus concerning the impact of storage time. However, other results indicated a significant influence of storage temperature on the variation in vitamin C levels for both citrus and hybrid varieties (P < 0.001). Depending on the genotype, tangerine had significantly lower vitamin C content compared to other varieties, at 16.9 mg/100 mL, with vitamin C ranging from 13.2 to 20.9 mg/100 mL (P < 0.001). The highest vitamin C content was found in lemon and hybrid varieties, around 65.5 (range 59.3-76.2) and 48.3 (range 29.6-75.5), respectively, all in mg/100 mL (P < 0.001). Furthermore, there was a tendency for decreasing vitamin C concentration due to temperature (P = 0.078), while citrus variety experienced a decrease, although not significant. The ideal temperature (15 °C) and duration of storage (56 days) to minimize vitamin C loss in citrus fruits are at their optimum point. In conclusion, the deterioration of vitamin C in citrus fruits is influenced by both temperature and storage duration, and its content is also impacted by the variety of citrus.

Investigating the impact of storage duration and temperature on vitamin C in various citrus genotypes: A meta-analysis method.

The present work disseminates a solid scientific meta-analysis method to investigate the impact of storage duration and temperature on vitamin C of citrus. This work is initiated by designing of the PICO framework, collecting, and organizing the articles, creating selection criteria, sorting articles, identifying factors influencing moderation effects and sources of diversity, tabulating data, and employing analysis in the form of a linear mixed model. Using this method, we identified 54 distinct studies from a pool of 289 eligible peer-reviewed publications, focusing on variations of vitamin C in citrus. The method provides mean values in both quadratic and linear regression forms.•This method provides a detailed description starting from topic selection to statistical methodologies intended for performing meta-analysis.•All guidelines for conducting this method have been approved by all authors and adhere to the standard PRISMA-P guidelines.•Disseminating this method in a peer-reviewed publication aims to facilitate scholarly discussions and promote transparency, ultimately improving the standard for performing meta-analysis on vitamin C levels in citrus concerning various genotypes, storage temperatures, and durations.

An overview of ethylene insensitive tomato mutants: Advantages and disadvantages for postharvest fruit shelf-life and future perspective.

The presence of ethylene during postharvest handling of tomatoes can be the main problem in maintaining fruit shelf-life by accelerating the ripening process and causing several quality changes in fruit. Several researchers have studied the methods for improving the postharvest life of tomato fruit by controlling ethylene response, such as by mutation. New ethylene receptor mutants have been identified, namely Sletr1-1, Sletr1-2, Nr (Never ripe), Sletr4-1, and Sletr5-1. This review identifies the favorable and undesirable effects of several ethylene receptor mutants. Also, the impact of those mutations on the metabolite alteration of tomatoes and the future perspectives of those ethylene receptor mutants. The review data is taken from the primary data of our experiment related to ethylene receptor mutants and the secondary data from numerous publications in Google Scholar and other sources pertaining to ethylene physiology. This review concluded that mutation in the SlETR1 gene was more effective than mutation in NR, SLETR4, and SLETR5 genes in generating a new ethylene mutant. Sletr1-2 mutant is a potential ethylene receptor mutant for developing new tomato cultivars with prolonged fruit-shelf life without any undesirable effect. Therefore, that has many challenges to using the Sletr1-2 mutant for future purposes in breeding programs.

SlIAA9 Mutation Maintains Photosynthetic Capabilities under Heat-Stress Conditions.

Tomato is one of the most widely consumed horticultural products. However, tomato is very sensitive to changes in temperature. Daily average temperatures above 32 °C severely reduced tomato plant growth, development, and productivity. Therefore, climate change-induced global warming is a major threat to future tomato production. Good photosynthetic capability under heat stress conditions is known to be a major sign of heat tolerance. Tomato INDOLE-ACETIC-ACID (SlIAA9) is a transcriptional repressor in auxin signaling. SlIAA9 mutation caused heightened endogenous auxin response and biosynthesis within plant tissues. In this study, we studied the photosynthetic capability of iaa9-3 and iaa9-5 mutants under heat-stress conditions. We discovered that both iaa9-3 and iaa9-5 could maintain their photosynthetic capability after 14 days of heat treatment (>40 °C), differing from Wild Type-Micro-Tom (WT-MT) tomato. Both iaa9 mutants had higher net photosynthetic rate, stomatal conductance, leaf total chlorophyll, leaf carotenoids, Fv/Fm value, and lower leaf MDA than WT-MT. These results suggested that the SlIAA9 mutation benefits plant adaptation to heat stress.

Transcriptome dataset from Solanum lycopersicum L. cv. Micro-Tom; wild type and two mutants of INDOLE-ACETIC-ACID (SlIAA9) using long-reads sequencing oxford nanopore technologies.

OBJECTIVE: Tomatoes are the most widely consumed fruit vegetable and are relatively easy to cultivate. However, an increase in temperature causes some plants to respond with a decrease in fruit production. So, it is necessary to develop plants resistant to extreme temperature changes. The tomato cv. Micro-Tom has genetic variations in the gene of INDOLE-ACETIC-ACID, namely SlIAA9-3 and SlIAA9-5. However, the genetic information regarding the full-length transcript of the gene from this type of tomato plant is unknown. Therefore, this study aimed to determine the full-length transcript of the genes of these three types of tomatoes using long-reads sequencing technology from Oxford Nanopore. DATA DESCRIPTION: The total RNA from three types of Micro-Tom was isolated with the RNeasy PowerPlant Kit. Then, the RNA sequencing process used PCR-cDNA Barcoding kit - SQK-PCB109 and continued with the processing of raw reads based on the protocol from microbepore protocol ( https://github.com/felixgrunberger/microbepore ). The resulting raw reads were 578 374, 409 905, and 851 948 for wildtype, iaa9-3, and iaa9-5, respectively. After obtaining cleaned reads, each sample was mapped to the tomato reference genome (S. lycopersicum ITAG4.0) with the Minimap2 program. In particular, 965 genes were expressed only in the iaa9-3 mutant, and 2332 genes were expressed only in the iaa9-5 mutant. Whereas in the wild type, 1536 genes are specifically expressed. In cluster analysis using the heatmap analysis, separate groups were obtained between the wild type and the two mutants. This proves an overall difference in transcript levels between the wild type and the mutants.

Parthenocarpic tomato mutants, iaa9-3 and iaa9-5, show plant adaptability and fruiting ability under heat-stress conditions.

Fruit set is one of the main problems that arise in tomato plants under heat-stress conditions, which disrupt pollen development, resulting in decreased pollen fertility. Parthenocarpic tomatoes can be used to increase plant productivity during failure of the fertilisation process under heat-stress conditions. The aim of this study were to identify the plant adaptability and fruiting capability of ?iaa9-3 and iaa9-5 tomato mutants under heat-stress conditions. The iaa9-3 and iaa9-5 and wild-type Micro-Tom (WT-MT) plants were cultivated under two temperature conditions: normal and heat-stress conditions during plant growth. The results showed that under the heat-stress condition, iaa9-3 and iaa9-5 showed delayed flowering time, increased number of flowers, and increased fruit set and produced normal-sized fruit. However, WT-MT cannot produce fruits under heat stress. The mutants can grow under heat-stress conditions, as indicated by the lower electrolyte leakage and H2O2 concentration and higher antioxidant activities compared with WT-MT under heat-stress conditions. These results suggest that iaa9-3 and iaa9-5 can be valuable genetic resources for the development of tomatoes in high-temperature environmental conditions.

The Role of Moringa Leaf Extract as a Plant Biostimulant in Improving the Quality of Agricultural Products.

Ensuring high-quality agricultural products has become important in agriculture since society's standard of living has risen. Meanwhile, Moringa oleifera L. leaf extract (MLE) has been used as a plant biostimulant to improve product quality. The effectiveness of MLE is associated with its beneficial components, consisting of nutrients, phytohormones, secondary metabolites, amino acids, and bioactive compounds. Previous studies have been carried out to find the effects of MLE application on the quality of different crops, including basil, kale, spinach, maize, radish, brinjal, pepper, tomato, grape, strawberry, and more. The results are generally positive concerning physical, nutritional, and chemical qualities. This review comprises recent findings regarding MLE application as a plant biostimulant to increase quality attributes, with its underlying mechanism.

Dataset on the combination effects of 1-methylcyclopropene and salicylic acid on postharvest quality of cut roses 'Peach Avalanche' and 'Sexy Red'.

Cut roses is one of the important ornamental cut flowers. Many factors affect the loss of postharvest life quality of cut flower, such as temperature, humidity and the presence of ethylene during postharvest storage. In ethylene sensitive roses, ethylene enhances petal discoloration, chlorophyll degradation, petal efflorescence and increase in petal diameter which generating in flower senescence. This data article described the effect of 1-Methylcyclopropene (1-MCP), Salicylic Acid (SA) and its combination on the postharvest life quality of two commercial cut roses varieties, 'Peach Avalanche' and 'Sexy Red'. The flower longevity quality of 1-MCP at 0.5 and 1 µL/L, SA 1.0 and 1.5%, combination of 1-MCP + SA (0.5 + 1.0 µL/L and 1.0 + 1.5%), and untreated flowers were analyzed.

Dataset on the change of postharvest quality of Physalis peruviana L. as an effect of ethylene inhibitor.

Ethylene will be a major problem in postharvest quality of fresh fruit such as Physalis peruviana L. that belongs to an important medicinal plant. This data article describes the alteration of fruit quality of P. peruviana L. during 21 days of postharvest storage as an effect of ethylene inhibitor, 1-Methylcyclopropene (1-MCP), at different concentration and duration of application. Nutritional parameters and fruit shelf life from 1-MCP treated fruit with three level concentrations (0.5, 1.0, and 2.0 µL L-1) and three level of durations application (6, 12 and 24 hours) were analyzed.

Data on the yield and quality of organically hybrids of tropical tomato fruits at two stages of fruit maturation.

Organic and traditional cultivation techniques significantly affect the yield and quality of tomato fruit. To achieve the highest possible production of hybrid lines, the appropriate cultivation system is needed. The application of different cultivation systems was expected to improve the yield and fruit quality of three new tropical hybrid tomatoes varieties that prolong fruit shelf life. This experiment was conducted to identify the effect of the different cultivation systems on the yield and fruit quality of three hybrid tomatoes from different tropical parental backgrounds ('Mutiara', 'Intan' and 'Ratna'). Those hybrid lines were cultivated with two farming systems (organic and conventional cultivation system), and the fruit quality was analysed at two stages of fruit maturation (Breaker and Red).

Data on the vegetative growth at post acclimatization stage of two dendrobium genotypes as an effect of different growing media.

The growing medium is an important factor for plant growth and development. Many growing media are used for orchids, but their availability is limited and some are prohibitively expensive. Therefore, alternative growing media need to be studied. This study was conducted to investigate the potency of some alternative growing media for growing two Dendrobium genotypes, D. sylvanum and D. nindii x D. stratiotes, at the post-acclimatization stage. Five growing media were used in this experiment, namely tree fern fibers, coconut fibers, sphagnum moss, asplenium root, and calliandra humus.

Potential Use of a Weak Ethylene Receptor Mutant, Sletr1-2, as Breeding Material To Extend Fruit Shelf Life of Tomato.

Mutations in the ethylene receptor gene (SlETR1), Sletr1-1 and Sletr1-2, are effective in reducing ethylene sensitivity and improving fruit shelf life. In this study the effect of Sletr1-1 and Sletr1-2 mutations was investigated in F1 hybrid lines. These two mutants and control were crossed with four commercial pure-line tomatoes. The Sletr1-1 mutation showed undesirable pleiotropic effects in the F1 hybrid lines. The Sletr1-2 mutation was effective in improving fruit shelf life of F1 hybrid lines for 4-5 days longer. It was also effective in improving fruit firmness without change in fruit size, ethylene production, respiration rate, and total soluble solids or a great reduction in fruit color, lycopene, and ß-carotene, although the titratable acidity was increased by Sletr1-2 mutation. These results indicate that the Sletr1-2 mutant allele has the potential to improve fruit shelf life via incorporation in tomato breeding programs.