Physiologic effects of three different protocols of isoflurane anesthesia in captive brown brocket deer (Mazama gouazoubira).

This study evaluates the cardiorespiratory and physiologic effects of three different protocols of chemical restraint using isoflurane in captive brown brocket deer (Mazama gouazoubira). Six adult deer, two males and four females, aged 3-6 yr old and weighing 16.3 +/- 1.5 kg (mean +/- SD), were used. The deer were physically restrained and anesthetized using one of three protocols: protocol 1 (P1), anesthesia was induced directly with isoflurane using a facemask and maintained for 1 hr with isoflurane delivered by endotracheal tube; protocol 2 (P2), oral premedication with midazolam was followed 1 hr later by induction and maintenance of anesthesia with isoflurane, as in P1; and protocol 3 (P3), intravenous anesthesia using a combination of ketamine, xylazine, and atropine was followed by isoflurane anesthesia, as in P1. Cardiorespiratory variables were recorded during physical restraint (TO); 5 min after intubation (T5); and every 10 min during anesthesia (T15-T55). Venous and arterial blood gas analysis was conducted at TO (venous blood only), T15, T35, and T55. Data were analyzed by the appropriate statistical tests. P values < 0.05 were considered significant. Under P1, three deer regurgitated during induction and one of them died because of aspiration pneumonia. The end-tidal isoflurane concentration differed significantly between P1, P2, and P3 (2.6 +/- 0.2, 2.3 +/- 0.5, and 0.9 +/- 0.3%, respectively). Hypotension was observed during anesthesia (T15-T55). Respiratory acidosis occurred at all times and protocols, and pH was significantly lower in P1 compared to P2 and P3. Metabolic acidosis was also observed following physical restraint (T0-T35) in all protocols. The use of isoflurane via facemask for anesthesia induction is not a safe method in deer that are not premedicated. Isoflurane used alone or in combination with midazolam and ketamine-xylazine-atropine combination causes hypotension and dose-dependent respiratory acidosis.

Carotid artery exteriorization in brown brocket deer (Mazama gouazoubira) for an experimental study of anesthesia.

This report evaluates the carotid artery exteriorization technique to allow repeated percutaneous artery catheterization in six brown brocket deer (Mazama gouazoubira). Repeated percutaneous catheterization of the carotid artery was possible for periods of 3 mo to obtain arterial blood and monitor arterial blood pressure of deer without risk of arterial rupture. The artery pulse was easily palpable for periods up to 15 mo. Postoperative complication and/or arterial damage was not observed.

Effects of physical and chemical immobilization on hematologic and biochemical variables in captive brown brocket deer (Mazama gouazoubira).

BACKGROUND: Alterations in cellular and chemical constituents in blood are indicators of physical or chemical restraint in deer. OBJECTIVES: The aim of this study was to determine the effects of physical and chemical restraint on hematologic and biochemical analytes in captive brown brocket deer (Mazama gouazoubira). METHODS: Six adult deer were physically restrained and randomly anesthetized in a crossover design using 3 protocols (P). In P1, anesthesia was induced directly with isoflurane using a face mask and was maintained with isoflurane delivered by endotracheal tube. In P2, oral premedication with midazolam was followed 1 hour later by induction and maintenance of anesthesia with isoflurane, as in P1. In P3, intravenous anesthesia with a combination of ketamine, xylazine, and atropine was followed by anesthesia with isoflurane, as in P1. Blood was collected immediately after physical restraint (T0) and during anesthesia at 20-minute intervals (T15, T35, and T55). Hematologic and biochemical constituents were determined using manual methods or automated analyzers, with cortisol measured by enzyme immunoassay. RESULTS: During physical restraint, following food and water restriction, significant hemoconcentration, increased WBC count, and lower cortisol concentration were observed compared with results at T15, T35, and T55. During anesthesia, lymphocyte count was lower in P2 than P1, urea concentration was decreased at T15 using P2, and plasma glucose concentration was increased after T0 (P<.01). Among the anesthetic protocols, there was a trend for decreased cortisol concentration using P2. CONCLUSIONS: Physical restraint and anesthesia affect some blood constituents in captive brown brocket deer, and midazolam premedication may modify the intensity of the stress response.

Ovário-histerectomia vídeo-assistida com único portal em cadelas: estudo retrospectivo de 20 casos / Single-port video-assisted ovariohysterectomy in bitches: retrospective study of 20 cases

O objetivo do presente estudo foi avaliar retrospectivamente as 20 primeiras ovário-histerectomias vídeo-assistidas com único portal (SPVA-OSH), realizadas por um cirurgião não proficiente nessa técnica. Vinte cadelas foram submetidas à laparoscopia para SPVA-OSH, com o auxílio de um endoscópio de 10mm, com canal de trabalho de 5mm, inserido por um trocarte, posicionado na região pré-púbica, e coagulação bipolar. O tempo cirúrgico médio foi de 22,95±5,17 minutos. Os cinco primeiros procedimentos consumiram mais tempo cirúrgico do que os demais. A abordagem aos pedículos ovarianos direito e esquerdo foram as etapas que apresentaram maior tempo de execução. Houve complicação leve ou moderada em seis (30 por cento) das 20 cadelas operadas. As complicações foram: hemorragia leve ou moderada em um dos pedículos ovarianos em quatro (20 por cento) cadelas e hemorragia grave, devido à punção do baço com agulha de Veress em dois (10 por cento) animais. A SPVA-OSH foi realizada em cadelas por um cirurgião no início da curva de aprendizado, sem complicações maiores.

The aim of the present study was to evaluate retrospectively the first 20 cases of single-port video-assisted ovariohysterectomy (SPVA-OSH) performed by an inexperienced surgeon. Twenty bitches submitted to SPVA-OSH were assessed. A 10mm operative rigid endoscope with one 5 mm working channel and bipolar coagulation was used. The trocar was positioned in the prepubic area of the abdomen. Surgical time and complication rates were assessed descriptively. Mean surgical time was of 22.95±5.17 minutes. Mild or moderate complications were present in six (30 percent) out of 20 bitches that were operated. The first five surgical procedures spent more time to be performed than the others. The longest transoperative momentum time of execution was spent in the approach to the left and right ovarian pedicles. The complications were: mild or moderate hemorrhage from one of the ovarian pedicles in four bitches (20 percent) and severe hemorrhage due to accidental puncture of the spleen with Veress needle in two animals (10 percent). SPVA-OSH was performed in bitches by an inexperienced surgeon, without severe complications.

Effects of laparoscopy on the cardiorespiratory system of brown brocket deer (Mazama gouazoubira) anesthetized with ketamine/ xylazine/ midazolam combination and isoflurane / Efeitos da laparoscopia sobre o sistema cardiorrespiratório de veados-catingueiro (Mazama gouazoubira) anestesiados com a associação cetamina/xilazina/midazolam e isofluorano

Laparoscopy is not widely used as a tool to perform assisted reproduction techniques in South American cervids; thus, scarce information in literature is available regarding its effects and appropriate anesthetic protocols to perform it. This study evaluated the effect of laparoscopy on heart rate (HR), respiration rate (RR), saturation of oxyhemoglobin (SpO2) and rectal temperature (RT) of six female brown brocket deer (Mazama gouazoubira) anesthetized with ketamine (5mg/kg), xylazine (0.3mg/kg), midazolam (0.5mg/kg) combination i.v. and isoflurane. Twelve laparoscopies were performed and each animal was used twice with a 40-day interval. After anesthetized, the animals were placed in dorsal recumbency to perform laparoscopy procedure using abdominal CO2 insufflations (14.2 ± 2.39mmHg; M ± SE). The main events of the laparoscopy procedure were divided into three periods: animal without (P1) and with abdominal insufflation (P2) and abdominal insufflation with the hips raised at 45º (P3). As a control, the animals were anesthetized again 40 days after the last laparoscopy, and were maintained in a dorsal recumbency for the same average duration of the previous anesthesia and no laparoscopy procedure was conducted. The period of anesthesia for the controls was also divided into P1, P2, and P3 considering the average duration of these periods in previous laparoscopies performed. Data were analyzed through the (ANOVA) variance analysis followed by Tukey test and values at P<0.05 were considered significant. No significant differences were observed in the parameters evaluated at P1, P2 and P3 between the animals submitted to laparoscopy and control. However, the RR mean between P1 (38.8 ± 4.42) and P3 (32.7 ± 4.81); and the RT mean between the P1 (38.2ºC ± 0.17), P2 (37.6ºC ± 0.19) and P3 (37.0ºC ± 0.21) varied significantly, independent of the laparoscopy. These data indicated that laparoscopy didn't cause any significant alterations in the...

A laparoscopia ainda é pouco utilizada como ferramenta para técnicas de reprodução assistida em cervídeos sul-americanos, não havendo informações sobre seus efeitos e protocolos anestésicos seguros para sua realização. Objetivaramse avaliar as possíveis alterações na freqüência cardíaca (FC), respiratória (FR), saturação de oxihemoglobina (SpO2) e temperatura retal (TR) durante a laparoscopia para visualização dos órgãos reprodutivos de seis fêmeas de veado-catingueiro (Mazama gouazoubira) anestesiadas com a associação cetamina (5mg/kg), xilazina (0,3mg/kg), midazolam (0,5mg/kg) e isofluorano. Cada animal, após anestesiado, foi posicionado em decúbito dorsal para realização de duas laparoscopias com insuflação abdominal de CO2 (14,2 ± 2,39mmHg; M ± EPM) com intervalo de 40 dias. Para avaliar os principais eventos da laparoscopia, esta foi dividida em três períodos: animal sem insuflação abdominal (P1), com insuflação abdominal (P2) e insuflação abdominal com os quadris elevados a 45º (P3). O controle foi realizado após 40 dias da última laparoscopia, para isto, cada animal foi novamente anestesiado e mantido em decúbito dorsal por um período de tempo igual ao tempo médio de duração das anestesias realizadas nas laparoscopias, sem que o procedimento laparoscópico fosse realizado. O tempo de anestesia dos controles foi também dividido em P1, P2 e P3, respeitando o tempo médio de duração de cada um destes períodos das laparoscopias. Para análise dos dados foi usado o teste de análise de variância (ANOVA) seguido do teste de Tukey e valores de P<0,05 considerados significativos. Não houve diferença significativa nos parâmetros estudados em nenhum dos períodos estabelecidos para o controle e laparoscopia. Porém, a FR média entre P1 (38,8 ± 4,42) e P3 (32,7 ± 4,81) e a TR média entre P1 (38,2ºC ± 0,17), P2 (37,6ºC ± 0,19) e P3 (37,0ºC ± 0,21) variaram significativamente, independente da laparoscopia. Tais dados permitiram concluir que a laparoscopia...