RESUMO
Heterogeneity of central serotonin (5-HT) raphe neurons is suggested by numerous lines of evidence, but its genetic basis remains elusive. The transcription factor Pet1 is required for the acquisition of serotonergic identity in a majority of neurons in the raphe nuclei. Nevertheless, a subset of 5-HT neurons differentiates in Pet1 knock-out mice. We show here that these residual 5-HT neurons outline a unique subpopulation of raphe neurons with highly selective anatomical targets and characteristic synaptic differentiations. In Pet1 knock-out mice, 5-HT innervation strikingly outlines the brain areas involved in stress responses with dense innervation to the basolateral amygdala, the paraventricular nucleus of the hypothalamus, and the intralaminar thalamic nuclei. In these regions, 5-HT terminals establish asymmetric synaptic junctions. This target selectivity could not be related to altered growth of the remaining 5-HT neurons, as indicated by axon tracing and cell culture analyses. The residual 5-HT axon terminals are functional with maintained release properties in vitro and in vivo. The functional consequence of this uneven distribution of 5-HT innervation on behavior was characterized. Pet1 knock-out mice showed decreased anxiety behavior in novelty exploration and increased fear responses to conditioned aversive cues. Overall, our findings lead us to propose the existence of Pet1-dependent and Pet1-resistant 5-HT neurons targeting different brain centers that might delineate the anatomical basis for a dual serotonergic control on stress responses.
Assuntos
Regulação da Expressão Gênica no Desenvolvimento/genética , Neurônios/citologia , Núcleos da Rafe/citologia , Núcleos da Rafe/crescimento & desenvolvimento , Serotonina/fisiologia , Fatores de Transcrição/genética , Animais , Diferenciação Celular/genética , Feminino , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Camundongos Transgênicos , Neurogênese/genética , Neurônios/metabolismo , Estresse Psicológico/genética , Estresse Psicológico/patologia , Estresse Psicológico/fisiopatologia , Fatores de Transcrição/deficiência , Fatores de Transcrição/fisiologiaRESUMO
Spontaneous activity generated in the retina is necessary to establish a precise retinotopic map, but the underlying mechanisms are poorly understood. We demonstrate here that neural activity controls ephrin-A-mediated responses. In the mouse retinotectal system, we show that spontaneous activity of the retinal ganglion cells (RGCs) is needed, independently of synaptic transmission, for the ordering of the retinotopic map and the elimination of exuberant retinal axons. Activity blockade suppressed the repellent action of ephrin-A on RGC growth cones by cyclic AMP (cAMP)-dependent pathways. Unexpectedly, the ephrin-A5-induced retraction required cAMP oscillations rather than sustained increases in intracellular cAMP concentrations. Periodic photo-induced release of caged cAMP in growth cones rescued the response to ephrin-A5 when activity was blocked. These results provide a direct molecular link between spontaneous neural activity and axon guidance mechanisms during the refinement of neural maps.
Assuntos
AMP Cíclico/metabolismo , Periodicidade , Retina/fisiologia , Transdução de Sinais/fisiologia , Vias Visuais/fisiologia , 1-Metil-3-Isobutilxantina/farmacologia , Potenciais de Ação/efeitos dos fármacos , Potenciais de Ação/fisiologia , Animais , Embrião de Mamíferos , Efrina-A5/metabolismo , Antagonistas de Aminoácidos Excitatórios/farmacologia , Cones de Crescimento/efeitos dos fármacos , Cones de Crescimento/fisiologia , Camundongos , Camundongos Knockout , Proteínas Munc18/deficiência , Técnicas de Cultura de Órgãos , Inibidores de Fosfodiesterase/farmacologia , Quinoxalinas/farmacologia , Retina/citologia , Células Ganglionares da Retina/citologia , Células Ganglionares da Retina/efeitos dos fármacos , Células Ganglionares da Retina/fisiologia , Tetrodotoxina/farmacologia , Vias Visuais/citologiaRESUMO
Proper maternal care is an essential factor of reproductive success in mammals, involving a repertoire of behaviors oriented toward the feeding and care of the offspring. Among the neurotransmitters involved in the initiation of these behaviors, serotonin (5-HT) seems to play an important role. Here we compared pup-oriented maternal behaviors in mice with constitutive 5-HT depletion, the tryptophan hydroxylase 2-knock-out (Tph2-KO) and the Pet1-KO mice. We report that the only common pup-oriented defect in these 2 hyposerotoninergic models is a defective nursing in parturient mice and altered nursing-like (crouching) behavior in virgin mice, while pup retrieval defects are only present in Tph2-KO. Despite a normal mammary gland development and milk production, the defect in appropriate nursing is responsible for severe growth retardation and early lethality of pups born to hyposerotonergic dams. This nursing defect is due to acute rather constitutive 5-HT depletion, as it is reproduced by adult knockdown of Tph2 in the dorsal raphe nucleus in mothers with a prior normal maternal experience. We conclude that 5-HT innervation from the dorsal raphe is required for both the initiation and maintenance of a normal nursing behavior. Our findings may be related to observations of reduced maternal/infant interactions in human depression.
Assuntos
Núcleo Dorsal da Rafe/metabolismo , Comportamento Materno , Serotonina/metabolismo , Transmissão Sináptica , Animais , Animais Recém-Nascidos , Feminino , Masculino , Camundongos , Camundongos Knockout , Serotonina/genética , Triptofano Hidroxilase/deficiência , Triptofano Hidroxilase/metabolismoRESUMO
White matter axons organize into fascicles that grow over long distances and traverse very diverse environments. The molecular mechanisms preserving this structure of white matter axonal tracts are not well known. Here, we used the optic nerve as a model and investigated the role of TAG-1, a cell adhesion molecule expressed by retinal axons. TAG-1 was first expressed in the embryonic retinal ganglion cells (RGCs) and later in the postnatal myelin-forming cells in the optic nerve. We describe the consequences of genetic loss of Tag-1 on the developing and adult retinogeniculate tract. Tag-1-null embryos display anomalies in the caliber of RGC axons, associated with an abnormal organization of the astroglial network in the optic nerve. The contralateral projections in the lateral geniculate nucleus are expanded postnatally. In the adult, Tag-1-null mice show a loss of RGC axons, with persistent abnormalities of axonal caliber and additional cytoskeleton and myelination defects. Therefore, TAG-1 is an essential regulator of the structure of RGC axons and their surrounding glial cells in the optic nerve.
Assuntos
Axônios/fisiologia , Moléculas de Adesão Celular Neuronais/fisiologia , Bainha de Mielina/metabolismo , Nervo Óptico/metabolismo , Retina/citologia , Células Ganglionares da Retina/citologia , Animais , Animais Recém-Nascidos , Axônios/ultraestrutura , Moléculas de Adesão Celular Neuronais/deficiência , Células Cultivadas , Contactina 2 , Embrião de Mamíferos , Regulação da Expressão Gênica no Desenvolvimento/fisiologia , Complexo Antígeno L1 Leucocitário/genética , Complexo Antígeno L1 Leucocitário/metabolismo , Camundongos , Camundongos Knockout , Proteínas do Tecido Nervoso/genética , Proteínas do Tecido Nervoso/metabolismo , Neuroglia/metabolismo , Nervo Óptico/ultraestrutura , Células Ganglionares da Retina/metabolismo , Células Ganglionares da Retina/ultraestrutura , Fator de Transcrição Brn-3A/genética , Fator de Transcrição Brn-3A/metabolismoRESUMO
Serotonin is a neurotransmitter involved in many psychiatric diseases. In humans, a lack of 5-HT2B receptors is associated with serotonin-dependent phenotypes, including impulsivity and suicidality. A lack of 5-HT2B receptors in mice eliminates the effects of molecules that directly target serotonergic neurons including amphetamine derivative serotonin releasers, and selective serotonin reuptake inhibitor antidepressants. In this work, we tested the hypothesis that 5-HT2B receptors directly and positively regulate raphe serotonin neuron activity. By ex vivo electrophysiological recordings, we report that stimulation by the 5-HT2B receptor agonist, BW723C86, increased the firing frequency of serotonin Pet1-positive neurons. Viral overexpression of 5-HT2B receptors in these neurons increased their excitability. Furthermore, in vivo 5-HT2B-receptor stimulation by BW723C86 counteracted 5-HT1A autoreceptor-dependent reduction in firing rate and hypothermic response in wild-type mice. By a conditional genetic ablation that eliminates 5-HT2B receptor expression specifically and exclusively from Pet1-positive serotonin neurons (Htr2b 5-HTKO mice), we demonstrated that behavioral and sensitizing effects of MDMA (3,4-methylenedioxy-methamphetamine), as well as acute behavioral and chronic neurogenic effects of the antidepressant fluoxetine, require 5-HT2B receptor expression in serotonergic neurons. In Htr2b 5-HTKO mice, dorsal raphe serotonin neurons displayed a lower firing frequency compared to control Htr2b lox/lox mice as assessed by in vivo extracellular recordings and a stronger hypothermic effect of 5-HT1A-autoreceptor stimulation was observed. The increase in head-twitch response to DOI (2,5-dimethoxy-4-iodoamphetamine) further confirmed the lower serotonergic tone resulting from the absence of 5-HT2B receptors in serotonin neurons. Together, these observations indicate that the 5-HT2B receptor acts as a direct positive modulator of serotonin Pet1-positive neurons in an opposite way as the known 5-HT1A-negative autoreceptor.
Assuntos
Sensibilização do Sistema Nervoso Central/fisiologia , Indóis/farmacologia , Núcleos da Rafe/fisiologia , Receptor 5-HT2B de Serotonina/fisiologia , Neurônios Serotoninérgicos/fisiologia , Tiofenos/farmacologia , 3,4-Metilenodioxianfetamina/farmacologia , 8-Hidroxi-2-(di-n-propilamino)tetralina/farmacologia , Potenciais de Ação/efeitos dos fármacos , Potenciais de Ação/fisiologia , Anfetaminas/farmacologia , Animais , Temperatura Corporal/efeitos dos fármacos , Feminino , Fluoxetina/farmacologia , Masculino , Camundongos , Camundongos Knockout , Camundongos Transgênicos , Neurogênese/fisiologia , Inibição Pré-Pulso/efeitos dos fármacos , Inibição Pré-Pulso/fisiologia , Receptor 5-HT2B de Serotonina/genética , Agonistas do Receptor 5-HT2 de Serotonina/farmacologia , Fatores de Transcrição/genéticaRESUMO
The calcium-stimulated adenylate cyclase 1 (AC1) has been shown to be required for the refinement of the retinotopic map, but the mechanisms involved are not known. To investigate this question, we devised a retinotectal coculture preparation that reproduces the gradual acquisition of topographic specificity along the rostrocaudal axis of the superior colliculus (SC). Temporal retinal axons invade the entire SC at 4 d in vitro (DIV) and eliminate exuberant branches caudally by 12 DIV. Temporal and nasal axons form branches preferentially in the rostral or caudal SC, respectively. Retinal explants from AC1-deficient mice, AC1(brl/brl), maintain exuberant branches and lose the regional selectivity of branching when confronted with wild-type (WT) SC. Conversely, WT retinas correctly target AC1(brl/brl) collicular explants. The effects of AC1 loss of function in the retina are mimicked by the blockade of ephrin-A5 signaling in WT cocultures. Video microscopic analyses show that AC1(brl/brl) axons have modified responses to ephrin-A5: the collapse of the growth cones occurs, but the rearward movement of the axon is arrested. Our results demonstrate a presynaptic, cell autonomous role of AC1 in the retina and further indicate that AC1 is necessary to enact a retraction response of the retinal axons to ephrin-A5 during the refinement of the retinotopic map.
Assuntos
Adenilil Ciclases/fisiologia , Axônios/enzimologia , Efrina-A5/fisiologia , Retina/enzimologia , Retina/crescimento & desenvolvimento , Adenilil Ciclases/biossíntese , Adenilil Ciclases/genética , Animais , Axônios/metabolismo , Axônios/ultraestrutura , Técnicas de Cocultura , Efrina-A5/antagonistas & inibidores , Feminino , Camundongos , Camundongos Knockout , Gravidez , Retina/diagnóstico por imagem , UltrassonografiaRESUMO
The membrane-trafficking pathway mediated by tetanus neurotoxin-insensitive vesicle-associated membrane protein (TI-VAMP) in neurons is still unknown. We show herein that TI-VAMP expression is necessary for neurite outgrowth in PC12 cells and hippocampal neurons in culture. TI-VAMP interacts with plasma membrane and endosomal target soluble N-ethylmaleimide-sensitive factor attachment protein receptors, suggesting that TI-VAMP mediates a recycling pathway. L1, a cell-cell adhesion molecule involved in axonal outgrowth, colocalized with TI-VAMP in the developing brain, neurons in culture, and PC12 cells. Plasma membrane L1 was internalized into the TI-VAMP-containing compartment. Silencing of TI-VAMP resulted in reduced expression of L1 at the plasma membrane. Finally, using the extracellular domain of L1 and N-cadherin immobilized on beads, we found that the silencing of TI-VAMP led to impaired L1- but not N-cadherin-mediated adhesion. Furthermore, TI-VAMP- but not synaptobrevin 2-containing vesicles accumulated at the site of the L1 bead-cell junction. We conclude that TI-VAMP mediates the intracellular transport of L1 and that L1-mediated adhesion controls this membrane trafficking, thereby suggesting an important cross talk between membrane trafficking and cell-cell adhesion.
Assuntos
Grânulos Citoplasmáticos/metabolismo , Molécula L1 de Adesão de Célula Nervosa/metabolismo , Neuritos/metabolismo , Neurônios/metabolismo , Proteínas de Transporte Vesicular , Animais , Transporte Biológico , Encéfalo/embriologia , Encéfalo/metabolismo , Caderinas/metabolismo , Caderinas/fisiologia , Compartimento Celular , Membrana Celular/metabolismo , Membrana Celular/fisiologia , Células Cultivadas , Grânulos Citoplasmáticos/fisiologia , Embrião de Mamíferos/metabolismo , Proteínas de Membrana/metabolismo , Proteínas de Membrana/fisiologia , Camundongos , Microscopia de Vídeo , Molécula L1 de Adesão de Célula Nervosa/fisiologia , Neuritos/fisiologia , Neurônios/fisiologia , Células PC12 , Ligação Proteica , Estrutura Terciária de Proteína , Proteínas R-SNARE , RNA Interferente Pequeno/farmacologia , Ratos , Proteínas SNARE , Transdução de Sinais , Frações SubcelularesRESUMO
Serotonin (5-HT) neurotransmission in the brain relies on a widespread axon terminal network originating from the hindbrain raphe nuclei. These projections are topographically organized such that the dorsal (DR), and median raphe (MnR) nuclei have different brain targets. However, the guidance molecules involved in this selective targeting in development are unknown. Here, we show the implication of ephrinA5 signaling in this process. We find that the EphA5 gene is selectively expressed in a subset of 5-HT neurons during embryonic and postnatal development. Highest coexpression of EphA5 and the 5-HT marker Tph2 is found in the DR, with lower coexpression in the MnR, and hardly any colocalization of the caudal raphe in the medulla. Accordingly, ephrinA induced a dose-dependent collapse response of 5-HT growth cones cultured from rostral but not caudal raphe. Ectopic expression of ephrinA3, after in utero electroporation in the amygdala and piriform cortex, repelled 5-HT raphe fiber ingrowth. Conversely, misplaced DR 5-HT axons were found in ephrin A5 knockout mice in brain regions that are normally only targeted by MnR 5-HT axons. This causes an overall increase in the density of 5-HT innervation in the ventromedial hypothalamus, the suprachiasmatic nucleus, and the olfactory bulb. All these brain areas have high expression of ephrinAs at the time of 5-HT fiber ingrowth. Present results show for the first time the role of a guidance molecule for the region-specific targeting of raphe neurons. This has important implications to understand how functional parsing of central 5-HT neurons is established during development.
Assuntos
Efrina-A5/metabolismo , Regulação da Expressão Gênica no Desenvolvimento/fisiologia , Núcleos da Rafe do Mesencéfalo/citologia , Prosencéfalo/citologia , Serotonina/metabolismo , Transdução de Sinais/fisiologia , Fatores Etários , Tonsila do Cerebelo/citologia , Tonsila do Cerebelo/metabolismo , Animais , Animais Recém-Nascidos , Células Cultivadas , Embrião de Mamíferos , Efrina-A5/genética , Efrinas/genética , Efrinas/metabolismo , Proteínas de Fluorescência Verde/genética , Proteínas de Fluorescência Verde/metabolismo , Camundongos , Camundongos Transgênicos , Vias Neurais/fisiologia , Prosencéfalo/metabolismo , Proteínas da Membrana Plasmática de Transporte de Serotonina/genética , Proteínas da Membrana Plasmática de Transporte de Serotonina/metabolismo , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo , Triptofano Hidroxilase/genética , Triptofano Hidroxilase/metabolismoRESUMO
Serotonin (5-HT) deficiency occurs in a number of brain disorders that affect cognitive function. However, a direct causal relationship between 5-HT hypo-transmission and memory and underlying mechanisms has not been established. We used mice with a constitutive depletion of 5-HT brain levels (Pet1KO mice) to analyze the contribution of 5-HT to different forms of learning and memory. Pet1KO mice exhibited a striking deficit in novel object recognition memory, a hippocampal-dependent task. No alterations were found in tasks for social recognition, procedural learning, or fear memory. Viral delivery of designer receptors exclusively activated by designer drugs was used to selectively silence the activity of 5-HT neurons in the raphe. Inhibition of 5-HT neurons in the median raphe, but not the dorsal raphe, was sufficient to impair object recognition in adult mice. In vivo electrophysiology in behaving mice showed that long-term potentiation in the hippocampus of 5-HT-deficient mice was altered, and administration of the 5-HT1A agonist 8-OHDPAT rescued the memory deficits. Our data suggest that hyposerotonergia selectively affects declarative hippocampal-dependent memory. Serotonergic projections from the median raphe are necessary to regulate object memory and hippocampal synaptic plasticity processes, through an inhibitory control mediated by 5-HT1A receptors.
Assuntos
Hipocampo/fisiologia , Memória/fisiologia , Plasticidade Neuronal , Neurônios Serotoninérgicos/fisiologia , Serotonina/fisiologia , Animais , Potenciais Pós-Sinápticos Excitadores , Feminino , Potenciação de Longa Duração , Masculino , Consolidação da Memória/fisiologia , Camundongos Knockout , Núcleos da Rafe/fisiologia , Receptor 5-HT1A de Serotonina/fisiologia , Reconhecimento Psicológico/fisiologia , Fatores de Transcrição/genéticaRESUMO
Serotoninergic innervation of the central nervous system is provided by hindbrain raphe nuclei (B1-B9). The extent to which each raphe subdivision has distinct topographic organization of their projections is still unclear. We provide a comprehensive description of the main targets of the rostral serotonin (5-HT) raphe subgroups (B5-B9) in the mouse brain. Adeno-associated viruses that conditionally express GFP under the control of the 5-HT transporter promoter were used to label small groups of 5-HT neurons in the dorsal (B7d), ventral (B7v), lateral (B7l), and caudal (B6) subcomponents of the dorsal raphe (DR) nucleus as well as in the rostral and caudal parts of the median raphe (MR) nucleus (B8 and B5, respectively), and in the supralemniscal (B9) cell group. We illustrate the distinctive and largely non-overlapping projection areas of these cell groups: for instance, DR (B7) projects to basal parts of the forebrain, such as the amygdala, whereas MR (B8) is the main 5-HT source to the hippocampus, septum, and mesopontine tegmental nuclei. Distinct subsets of B7 have preferential brain targets: B7v is the main source of 5-HT for the cortex and amygdala while B7d innervates the hypothalamus. We reveal for the first time the target areas of the B9 cell group, demonstrating projections to the caudate, prefrontal cortex, substantia nigra, locus coeruleus and to the raphe cell groups. The broad topographic organization of the different raphe subnuclei is likely to underlie the different functional roles in which 5-HT has been implicated in the brain. The present mapping study could serve as the basis for genetically driven specific targeting of the different subcomponents of the mouse raphe system.
Assuntos
Tronco Encefálico/fisiologia , Núcleos da Rafe do Mesencéfalo/fisiologia , Técnicas de Rastreamento Neuroanatômico/métodos , Prosencéfalo/fisiologia , Neurônios Serotoninérgicos/fisiologia , Regiões 5' não Traduzidas , Animais , Tronco Encefálico/citologia , Tronco Encefálico/metabolismo , Integrases/genética , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Núcleos da Rafe do Mesencéfalo/citologia , Núcleos da Rafe do Mesencéfalo/metabolismo , Vias Neurais/metabolismo , Vias Neurais/fisiologia , Prosencéfalo/citologia , Prosencéfalo/metabolismo , Neurônios Serotoninérgicos/metabolismo , Serotonina/metabolismo , Proteínas da Membrana Plasmática de Transporte de Serotonina/genéticaRESUMO
Serotonergic neurons of the raphe nuclei exhibit anatomical, neurochemical and elecrophysiological heterogeneity that likely underpins their specific role in multiple behaviors. However, the precise organization of serotonin (5-HT) neurons to orchestrate 5-HT release patterns throughout the brain is not well understood. We compared the electrophysiological and neurochemical properties of dorsal and median raphe 5-HT neurons projecting to the medial prefrontal cortex (mPFC), amygdala (BLA) and dorsal hippocampus (dHP), combining retrograde tract tracing with brain slice electrophysiology and single-cell RT-PCR in Pet1-EGFP mice. Our results show that 5-HT neurons projecting to the dHP and the mPFC and the BLA form largely non-overlapping populations and that BLA-projecting neurons have characteristic excitability and membrane properties. In addition, using an unbiased clustering method that correlates anatomical, molecular and electrophysiological phenotypes, we find that 5-HT neurons with projections to the mPFC and the dHP segregate from those projecting to the BLA. Single-cell gene profiling showed a restricted expression of the peptide galanin in the population of 5-HT neurons projecting to the mPFC. Finally, cluster analysis allowed identifying an atypical subtype of 5-HT neuron with low excitability, long firing delays and preferential expression of the vesicular glutamate transporter type 3. Overall, these findings allow to define correlated anatomical and physiological identities of serotonin raphe neurons that help understanding how discrete raphe cells subpopulations account for the heterogeneous activities of the midbrain serotonergic system.
Assuntos
Prosencéfalo/citologia , Núcleos da Rafe/citologia , Núcleos da Rafe/fisiologia , Neurônios Serotoninérgicos/citologia , Neurônios Serotoninérgicos/fisiologia , Potenciais de Ação , Tonsila do Cerebelo/citologia , Tonsila do Cerebelo/fisiologia , Animais , Hipocampo/citologia , Hipocampo/fisiologia , Camundongos , Camundongos Transgênicos , Vias Neurais/citologia , Vias Neurais/fisiologia , Técnicas de Rastreamento Neuroanatômico , Fenótipo , Córtex Pré-Frontal/citologia , Córtex Pré-Frontal/fisiologia , Análise de Célula ÚnicaRESUMO
cAMP occupies a strategic position to control neuronal responses to a large variety of developmental cues. We have analyzed the role of calcium-stimulated adenylate cyclase 1 (AC1) in the development of retinal topographic maps. AC1 is expressed in retinal ganglion cells (RGCs) from embryonic day 15 to adulthood with a peak during the first postnatal week. At that time, the other calcium-stimulated AC, AC8, is expressed in the superior colliculus (SC) but not in the RGCs. In mice of the barrelless strain, which carry an inactivating mutation of the AC1 gene, calcium-stimulated AC activity is reduced by 40-60% in the SC and retina. RGC projection maps were analyzed with a variety of anterograde and retrograde tracers. After an initially normal development until postnatal day 3, retinal fibers from the ipsilateral and contralateral eye fail to segregate into eye-specific domains in the lateral geniculate nucleus and the SC. Topographic defects in the fine tuning of the retinotectal and retinogeniculate maps are also observed with abnormalities in the confinement of the retinal axon arbors in the anteroposterior and mediolateral dimensions. This is attributable to the lack of elimination of misplaced axon collaterals and to the maintenance of a transient ipsilateral projection. These results establish an essential role of AC1 in the fine patterning of the retinal map. Calcium-modulated cAMP production in the RGCs could constitute an important link between activity-dependent changes and the anatomical restructuring of the retinal terminal arbors within central targets.
Assuntos
Adenilil Ciclases/fisiologia , Retina/enzimologia , Adenilil Ciclases/genética , Animais , Axônios/enzimologia , Axônios/fisiologia , Contagem de Células , Corpos Geniculados/citologia , Corpos Geniculados/embriologia , Corpos Geniculados/enzimologia , Corpos Geniculados/crescimento & desenvolvimento , Hibridização In Situ , Isoenzimas/genética , Isoenzimas/fisiologia , Camundongos , Camundongos Mutantes Neurológicos , RNA Mensageiro/análise , RNA Mensageiro/biossíntese , Retina/citologia , Células Ganglionares da Retina/citologia , Células Ganglionares da Retina/enzimologia , Colículos Superiores/citologia , Colículos Superiores/embriologia , Colículos Superiores/enzimologia , Colículos Superiores/crescimento & desenvolvimento , Vias Visuais/citologia , Vias Visuais/embriologia , Vias Visuais/enzimologia , Vias Visuais/crescimento & desenvolvimentoRESUMO
Type 1 and type 8 adenylate cyclases, AC1 and AC8, are membrane bound enzymes that produce cAMP in response to calcium entry and could thus control a large number of developmental processes. We provide a detailed spatiotemporal localization of these genes in the mouse brain during embryonic and postnatal life using in situ hybridization. AC1 gene expression begins early in embryonic life (before E13), and its expression is much more widespread than in adults. Transient expression of AC1 is found in the striatum, the dorsal thalamus, the trigeminal nerve nuclei, the Purkinje cells of the cerebellum, the interneurons of the hippocampus, and the retinal ganglion cells. In all these structures, the peak of AC1 gene expression occurs during early postnatal life, decreasing by P10. After P15, AC1 expression is confined to the hippocampus, the cerebral cortex, and to the granule cells of the cerebellum. AC8 gene expression also begins early in embryonic life (E12)--but in a more limited number of regions than in adults. AC8 expression is initially restricted to the epithalamus, the hypothalamus, the superior colliculus, the cerebellar anlage the proliferative zone of the rhombic lip, and the spinal cord. The expression increases and broadens during postnatal life, particularly in the thalamus and the cerebral cortex. A transient peak of AC8 expression is found in layer IV of the somatosensory cortex. Thus, AC1 and AC8 have an early developmental onset with complementary spatiotemporal distribution patterns: AC1 is most broadly distributed in embryonic life, whereas AC8 is most broadly expressed in adulthood. Transient expression of these genes designate areas that may be particularly sensitive to neural activity/calcium-modulated cAMP responses during development.
Assuntos
Adenilil Ciclases/metabolismo , Encéfalo/enzimologia , Regulação da Expressão Gênica no Desenvolvimento/fisiologia , Expressão Gênica/fisiologia , Adenilil Ciclases/classificação , Adenilil Ciclases/genética , Animais , Autorradiografia/métodos , Encéfalo/embriologia , Embrião de Mamíferos , Hibridização In Situ/métodos , Camundongos , Radioisótopos/metabolismoRESUMO
Programmed cell death is widespread during the development of the central nervous system and serves multiple purposes including the establishment of neural connections. In the mouse retina a substantial reduction of retinal ganglion cells (RGCs) occurs during the first postnatal week, coinciding with the formation of retinotopic maps in the superior colliculus (SC). We previously established a retino-collicular culture preparation which recapitulates the progressive topographic ordering of RGC projections during early post-natal life. Here, we questioned whether this model could also be suitable to examine the mechanisms underlying developmental cell death of RGCs. Brn3a was used as a marker of the RGCs. A developmental decline in the number of Brn3a-immunolabelled neurons was found in the retinal explant with a timing that paralleled that observed in vivo. In contrast, the density of photoreceptors or of starburst amacrine cells increased, mimicking the evolution of these cell populations in vivo. Blockade of neural activity with tetrodotoxin increased the number of surviving Brn3a-labelled neurons in the retinal explant, as did the increase in target availability when one retinal explant was confronted with 2 or 4 collicular slices. Thus, this ex vivo model reproduces the developmental reduction of RGCs and recapitulates its regulation by neural activity and target availability. It therefore offers a simple way to analyze developmental cell death in this classic system. Using this model, we show that ephrin-A signaling does not participate to the regulation of the Brn3a population size in the retina, indicating that eprhin-A-mediated elimination of exuberant projections does not involve developmental cell death.