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1.
Cell Tissue Res ; 387(2): 303-314, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34837110

RESUMO

Renal α2-adrenoceptors have been reported to play a role in the regulation of urinary output, renin secretion, and water and sodium excretion in the kidneys. However, the distribution of α2-adrenoceptor subtypes in the kidneys remains unclear. In this study, we aimed to investigate the localization of α2-adrenoceptor subtypes in rat kidneys using 8-week-old Sprague-Dawley rats. Immunofluorescence imaging revealed that both α2A- and α2B-adrenoceptors were expressed in the basolateral, but not apical, membrane of the epithelial cells of the proximal tubules. We also found that α2A- and α2B-adrenoceptors were not expressed in the glomeruli, collecting ducts, or the descending limb of the loop of Henle and vasa recta. In contrast, α2C-adrenoceptors were found to be localized in the glomeruli and lumen of the cortical and medullary collecting ducts. These results suggest that noradrenaline may act on the basement membrane of the proximal tubules through α2A- and α2B-adrenoceptors. Moreover, noradrenaline may be involved in the regulation of glomerular filtration and proteinuria through the induction of morphological changes in mesangial cells and podocytes via α2C-adrenoceptors. In the collecting ducts, urinary noradrenaline may regulate morphological changes of the microvilli through α2C-adrenoceptors. Our findings provide an immunohistochemical basis for understanding the cellular targets of α2-adrenergic regulation in the kidneys. This may be used to devise therapeutic strategies targeting α2-adrenoceptors.


Assuntos
Receptores Adrenérgicos alfa 2 , Roedores , Animais , Rim , Norepinefrina , Ratos , Ratos Sprague-Dawley , Receptores Adrenérgicos alfa 2/fisiologia
2.
Proc Natl Acad Sci U S A ; 114(37): E7697-E7706, 2017 09 12.
Artigo em Inglês | MEDLINE | ID: mdl-28847964

RESUMO

Cancer cells alter their metabolism for the production of precursors of macromolecules. However, the control mechanisms underlying this reprogramming are poorly understood. Here we show that metabolic reprogramming of colorectal cancer is caused chiefly by aberrant MYC expression. Multiomics-based analyses of paired normal and tumor tissues from 275 patients with colorectal cancer revealed that metabolic alterations occur at the adenoma stage of carcinogenesis, in a manner not associated with specific gene mutations involved in colorectal carcinogenesis. MYC expression induced at least 215 metabolic reactions by changing the expression levels of 121 metabolic genes and 39 transporter genes. Further, MYC negatively regulated the expression of genes involved in mitochondrial biogenesis and maintenance but positively regulated genes involved in DNA and histone methylation. Knockdown of MYC in colorectal cancer cells reset the altered metabolism and suppressed cell growth. Moreover, inhibition of MYC target pyrimidine synthesis genes such as CAD, UMPS, and CTPS blocked cell growth, and thus are potential targets for colorectal cancer therapy.


Assuntos
Adenoma/metabolismo , Neoplasias Colorretais/metabolismo , Proteínas Proto-Oncogênicas c-myc/metabolismo , Adenoma/genética , Animais , Carcinogênese/genética , Carcinogênese/metabolismo , Proliferação de Células/fisiologia , Neoplasias Colorretais/genética , Modelos Animais de Doenças , Feminino , Genes myc , Humanos , Masculino , Metabolômica/métodos , Camundongos , Proteínas Proto-Oncogênicas c-myc/genética , Pirimidinas/biossíntese , Transcriptoma
3.
Eur J Neurosci ; 50(1): 1700-1711, 2019 07.
Artigo em Inglês | MEDLINE | ID: mdl-30687962

RESUMO

Ca2+ -induced Ca2+ release (CICR) via type-3 ryanodine receptor enhances neurotransmitter release in frog motor nerve terminals. To test a possible role of synaptic vesicle in CICR, we examined the effects of loading of EGTA, a Ca2+ chelator, into synaptic vesicles and depolymerization of actin fibers. Intravesicular EGTA loading via endocytosis inhibited the ryanodine sensitive enhancement of transmitter release induced by tetanic stimulation and the associated rises in intracellular-free Ca2+ ([Ca2+ ]i : Ca2+ transients). Latrunculin A, a depolymerizer of actin fibers, enhanced both spontaneous and stimulation-induced transmitter release, but inhibited the enhancement of transmitter release elicited by successive tetanic stimulation. The results suggest a possibility that the activation of CICR from mobilized synaptic vesicles caused the enhancement of neurotransmitter release.


Assuntos
Actinas/efeitos dos fármacos , Compostos Bicíclicos Heterocíclicos com Pontes/farmacologia , Quelantes de Cálcio/farmacologia , Cálcio/metabolismo , Fenômenos Eletrofisiológicos , Neurônios Motores/metabolismo , Terminações Pré-Sinápticas/metabolismo , Canal de Liberação de Cálcio do Receptor de Rianodina/metabolismo , Transmissão Sináptica/efeitos dos fármacos , Vesículas Sinápticas/metabolismo , Tiazolidinas/farmacologia , Animais , Ácido Egtázico/farmacologia , Estimulação Elétrica , Ranidae
4.
Arterioscler Thromb Vasc Biol ; 36(11): 2158-2162, 2016 11.
Artigo em Inglês | MEDLINE | ID: mdl-27562915

RESUMO

OBJECTIVE: The purpose of this study was to determine the role of smooth muscle cell-derived hypoxia-inducible factor-1α (Hif-1α) in the pathogenesis of aortic aneurysms. APPROACH AND RESULTS: Control mice and smooth muscle cell-specific hypoxia-inducible factor-1α-deficient mice were infused with ß-aminopropionitrile for 2 weeks and angiotensin II for 6 weeks to induce aortic aneurysm formation. Mutant mice experienced increased levels of aneurysm formation of the thoracic or abdominal aorta with more severe elastin disruption, compared with control mice. Smooth muscle cell-specific hypoxia-inducible factor-1α deficiency did not affect matrix metalloproteinase-2 activity; however, the activity of lysyl oxidase and the levels of tropoelastin mRNA in the angiotensin II- and ß-aminopropionitrile-treated aortae, associated with elastin fiber formation, were suppressed. Furthermore, we observed reduced volumes of mature cross-linked elastin in the thoracoabdominal aorta after treatment with angiotensin II and ß-aminopropionitrile. CONCLUSIONS: Deficiency of smooth muscle cell-derived hypoxia-inducible factor-1α augments aortic aneurysms, accompanied by disruption of elastin fiber formation, but not changes of elastin fiber degradation.


Assuntos
Aneurisma da Aorta Abdominal/prevenção & controle , Aneurisma da Aorta Torácica/prevenção & controle , Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo , Músculo Liso Vascular/metabolismo , Miócitos de Músculo Liso/metabolismo , Aminopropionitrilo , Angiotensina II , Animais , Aorta Abdominal/metabolismo , Aorta Abdominal/patologia , Aorta Torácica/metabolismo , Aorta Torácica/patologia , Aneurisma da Aorta Abdominal/induzido quimicamente , Aneurisma da Aorta Abdominal/metabolismo , Aneurisma da Aorta Abdominal/patologia , Aneurisma da Aorta Torácica/induzido quimicamente , Aneurisma da Aorta Torácica/metabolismo , Aneurisma da Aorta Torácica/patologia , Células Cultivadas , Dilatação Patológica , Modelos Animais de Doenças , Tecido Elástico/metabolismo , Tecido Elástico/patologia , Predisposição Genética para Doença , Subunidade alfa do Fator 1 Induzível por Hipóxia/deficiência , Subunidade alfa do Fator 1 Induzível por Hipóxia/genética , Masculino , Camundongos Knockout , Músculo Liso Vascular/patologia , Miócitos de Músculo Liso/patologia , Fenótipo , Proteína-Lisina 6-Oxidase/metabolismo , Tropoelastina/genética , Tropoelastina/metabolismo , Remodelação Vascular
5.
Neuropathology ; 36(2): 115-24, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26607405

RESUMO

New findings on flow or drainage pathways of brain interstitial fluid and cerebrospinal fluid have been made. The interstitial fluid flow has an effect on the passage of blood-borne substances in the brain parenchyma, especially in areas near blood-brain barrier (BBB)-free regions. Actually, blood-borne substances can be transferred in areas with intact BBB function, such as the hippocampus, the corpus callosum, periventricular areas, and medial portions of the amygdala, presumably through leaky vessels in the subfornical organs or the choroid plexus. Increasing evidence indicates that dysfunction of the BBB function may play a significant role in the pathogenesis of vascular dementia. Accordingly, we have examined which insults seen in patients suffering from vascular dementia have an effect on the BBB using experimental animal models exhibiting some phenotypes of vascular dementia. The BBB in the hippocampus was clearly deteriorated in Mongolian gerbils exposed to acute ischemia followed by reperfusion and also in stroke-prone spontaneously hypertensive rats (SHRSP) showing hypertension. The BBB in the corpus callosum was clearly deteriorated in Wistar rats with permanent ligation of the bilateral common carotid arteries showing chronic hypoperfusion. The BBB in the hippocampus and the olfactory bulb was mildly deteriorated in aged senescence accelerated prone mice (SAMP8) showing cognitive dysfunction. The BBB in the hippocampus was mildly deteriorated in aged animals with hydrocephalus. Mild endothelial damage was seen in hyperglycemic db/db mice. In addition, mRNA expression of osteopontin, matrix metalloproteinase-13 (MMP-13), and CD36 was increased in vessels showing BBB damage in hypertensive SHRSP. As osteopontin, MMP-13 and CD36 are known to be related to brain injury and amyloid ß accumulation or clearance, BBB damage followed by increased gene expression of these molecules not only contributes to the pathogenesis of vascular dementia, but also bridges the gap between vascular dementia and Alzheimer's disease.


Assuntos
Barreira Hematoencefálica/patologia , Demência Vascular/patologia , Animais , Modelos Animais de Doenças , Humanos
6.
Int J Toxicol ; 34(4): 325-35, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26023052

RESUMO

The present study was conducted to examine the chronic effects of potassium octatitanate fibers (trade name TISMO; chemical formula K2O·6TiO2) on the mouse lung and thoracic cavity. This method of infusion was employed to examine the direct effects of the fibers to the pleura. In the present study, 52- and 65-week experiments were employed to examine the long-term chronic effects after infusion of fiber-shaped TISMO into the thoracic cavities of A/J mice. Following this infusion, TISMO fibers were observed in the alveoli, indicating penetration through the visceral pleura. The additional histopathological detection of TISMO fibers in the liver, spleen, kidneys, ovary, heart, bone marrow, and brain of TISMO-infused mice indicated migration of the fibers out from the thoracic cavity. Atypical mesothelial cells with severe pleural proliferation were observed, but malignant mesotheliomas were not detected. This study demonstrated that intrathoracic infusion of TISMO fiber did not cause malignant mesothelioma but did cause severe chronic inflammation and proliferation of pleural mesothelial cells.


Assuntos
Células Epiteliais/efeitos dos fármacos , Pleura/efeitos dos fármacos , Cavidade Torácica/efeitos dos fármacos , Titânio/toxicidade , Animais , Células Epiteliais/metabolismo , Feminino , Rim/efeitos dos fármacos , Rim/metabolismo , Fígado/citologia , Fígado/efeitos dos fármacos , Fígado/metabolismo , Pulmão/efeitos dos fármacos , Pulmão/metabolismo , Neoplasias Pulmonares/induzido quimicamente , Neoplasias Pulmonares/diagnóstico , Mesotelioma/induzido quimicamente , Mesotelioma/diagnóstico , Mesotelioma Maligno , Camundongos , Camundongos Endogâmicos A , Tamanho do Órgão/efeitos dos fármacos , Baço/efeitos dos fármacos , Baço/metabolismo , Cavidade Torácica/metabolismo , Testes de Toxicidade Crônica
7.
Biomedicines ; 12(4)2024 Mar 22.
Artigo em Inglês | MEDLINE | ID: mdl-38672064

RESUMO

The choroid plexus (CP) plays significant roles in secreting cerebrospinal fluid (CSF) and forming circadian rhythms. A monolayer of epithelial cells with tight and adherens junctions of CP forms the blood-CSF barrier to control the movement of substances between the blood and ventricles, as microvessels in the stroma of CP have fenestrations in endothelial cells. CP epithelial cells are equipped with several kinds of transporters and ion channels to transport nutrient substances and secrete CSF. In addition, junctional components also contribute to CSF production as well as blood-CSF barrier formation. However, it remains unclear how junctional components as well as transporters and ion channels contribute to the pathogenesis of neurodegenerative disorders. In this manuscript, recent findings regarding the distribution and significance of transporters, ion channels, and junctional proteins in CP epithelial cells are introduced, and how changes in expression of their epithelial proteins contribute to the pathophysiology of brain disorders are reviewed.

8.
Pathol Int ; 63(8): 398-407, 2013 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-23957915

RESUMO

H18-K24 of human apolipopotein CIII (Apo CIII) (HATKTAK) is an activator of the macromolecular activators of phagocytosis from platelets (MAPPs). Using a rabbit antibody against HATKTAK, we performed an immunohistochemical study of human platelets. Indirect ELISA showed that this antibody reacts with Apo CIII-derived peptides with a C-terminal of HATKTAK, but not with Apo CIII. Immunoelectron microscopy revealed that reaction of anti-HATKTAK antibody occurred in the pseudopods of activated platelets. In blood coagula produced from the peripheral blood and formalin-fixed after various incubation periods, reaction of this antibody with platelets appeared rapidly with a peak at 3 to 6 h of incubation, and then diminished gradually. Leukocytes in the blood coagula were stained strongly positive. In tissue sections, fresh thrombi and hemorrhages with slight fibrin formation revealed a positive response of platelets to anti-HATKTAK antibody, whereas older ones with leukocytic infiltration, fibrin formation and organization did not. In addition to platelets, endothelial cells and leukocytes were stained positive by anti-HATKTAK antibody. All of the positive reactions by anti-HATKTAK antibody disappeared or diminished by co-incubation with HATKTAK. In conclusion, the anti-HATKTAK antibody reveals platelets during the early phase of activation.


Assuntos
Especificidade de Anticorpos , Apolipoproteína C-III/imunologia , Plaquetas/imunologia , Hemorragia/sangue , Imunoglobulina G/imunologia , Trombose/sangue , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Animais , Apolipoproteína C-III/metabolismo , Plaquetas/metabolismo , Criança , Pré-Escolar , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Imuno-Histoquímica , Lactente , Recém-Nascido , Masculino , Pessoa de Meia-Idade , Coelhos , Adulto Jovem
9.
J Pharmacol Exp Ther ; 332(3): 1072-80, 2010 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-19940106

RESUMO

Treatment with angiotensin II type 1 receptor blockers (ARBs) is the first-line therapy for hypertensive patients with diabetic nephropathy. However, emerging clinical evidence indicates that mineralocorticoid receptor (MR) blockers have blood pressure-independent antiproteinuric effects. We sought to determine whether treatment with an MR blocker, eplerenone, enhances the effects of an ARB, telmisartan, on podocyte injury and proteinuria in type 2 diabetic Otsuka-Long-Evans-Tokushima-Fatty (OLETF) rats. From 20 to 50 weeks old, diabetic OLETF rats showed higher systolic blood pressure (SBP) and urinary protein excretion (U(protein)V) than nondiabetic control Long-Evans-Tokushima-Otsuka rats. At 50 weeks old, OLETF rats also showed glomerular sclerosis and podocyte injury, whereas nephrin and podocin mRNA levels in isolated glomeruli were significantly decreased. Treatment with telmisartan (3 mg/kg/day p.o.) decreased SBP and U(protein)V, increased nephrin and podocin mRNA levels, and attenuated glomerular sclerosis and podocyte injury. Eplerenone (100 mg/kg/day p.o.) did not alter SBP but elicited similar changes in renal parameters. However, greater reductions in U(protein)V and podocyte injury and greater increases in nephrin and podocin mRNA levels were observed in the combination treatment group. Hydralazine (25 mg/kg/day p.o.) decreased SBP but did not alter any renal parameters. These data indicate that MR blockade enhances the SBP-independent antiproteinuric effect of an ARB through inhibiting podocyte injury in type 2 diabetic rats.


Assuntos
Bloqueadores do Receptor Tipo 1 de Angiotensina II/farmacologia , Diabetes Mellitus Tipo 2/tratamento farmacológico , Antagonistas de Receptores de Mineralocorticoides , Podócitos/efeitos dos fármacos , Proteinúria/tratamento farmacológico , Bloqueadores do Receptor Tipo 1 de Angiotensina II/uso terapêutico , Animais , Benzimidazóis/farmacologia , Benzimidazóis/uso terapêutico , Benzoatos/farmacologia , Benzoatos/uso terapêutico , Diabetes Mellitus Tipo 2/patologia , Sinergismo Farmacológico , Eplerenona , Peptídeos e Proteínas de Sinalização Intracelular , Glomérulos Renais/efeitos dos fármacos , Glomérulos Renais/metabolismo , Masculino , Proteínas de Membrana/biossíntese , Podócitos/patologia , Proteinúria/etiologia , Ratos , Ratos Endogâmicos OLETF , Espironolactona/análogos & derivados , Espironolactona/farmacologia , Espironolactona/uso terapêutico , Telmisartan
10.
Histochem Cell Biol ; 133(6): 669-76, 2010 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-20458492

RESUMO

We previously reported that the blood-brain barrier (BBB) function was deteriorated in vessels located in the hippocampus in stroke-prone spontaneously hypertensive rats (SHRSP). In order to assess whether substances with oxidative stress such as amyloid-beta (Abeta) can be scavenged in the BBB-damaged vessels, we examined the gene expression of representative efflux and influx transporters of Abeta, such as low-density lipoprotein receptor (LDLR), LDL-related protein 1 (LRP1), and the receptor for advanced glycation end product (RAGE) in the hippocampus of SHRSP with the BBB impairment and Wistar Kyoto rats (WKY) without the impairment. Real-time quantitative reverse transcriptase-polymerase chain reaction analysis revealed that LDLR gene expression was increased in the samples of SHRSP compared with those of WKY, while there was no significant difference in LRP1 or RAGE gene expression between SHRSP and WKY. Western blot analysis revealed that the protein expression of LDLR was increased in the samples of SHRSP compared with those of WKY. Immunoelectron microscopic examination revealed that the LDLR expression was seen in the luminal and abluminal cytoplasmic membranes and vesicular structures of the endothelial cells and the cytoplasm of perivascular cells, especially in vessels with immunoreactivity of albumin showing increased vascular permeability. These findings suggest that the expression of LDLR was increased in the hippocampus of SHRSP compared with that of WKY and was seen in the luminal and abluminal cytoplasmic membranes and vesicular structures of endothelial cells, suggesting a role of LDLR in the vessels with BBB impairment.


Assuntos
Barreira Hematoencefálica , Regulação da Expressão Gênica , Ratos Endogâmicos SHR , Receptores de LDL/metabolismo , Acidente Vascular Cerebral , Animais , Barreira Hematoencefálica/patologia , Western Blotting , Modelos Animais de Doenças , Masculino , RNA Mensageiro/metabolismo , Ratos , Ratos Endogâmicos WKY , Receptores de LDL/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa
11.
J Hypertens ; 26(9): 1849-59, 2008 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-18698221

RESUMO

OBJECTIVES: Beneficial effects of angiotensin II type 1 receptor blockers have been indicated for patients with diabetic nephropathy. We investigated the effects of an angiotensin II type 1 receptor blocker, telmisartan, on intrarenal angiotensin II levels and the progression of albuminuria or glomerular injury in type 2 diabetic Otsuka Long-Evans Tokushima Fatty rats with microalbuminuria. METHODS AND RESULTS: Otsuka Long-Evans Tokushima Fatty rats were randomly treated with telmisartan (10 mg/kg/day, orally), hydralazine (25 mg/kg/day in drinking water) or vehicle from the initiation of albuminuria (13 weeks old). At this age, Otsuka Long-Evans Tokushima Fatty rats showed low but detectable albuminuria (1.0 +/- 0.1 mg/day) and higher systolic blood pressure, postprandial blood glucose and kidney angiotensin II levels than age-matched nondiabetic Long-Evans Tokushima Otsuka rats. At 35 weeks of age, vehicle-treated Otsuka Long-Evans Tokushima Fatty rats did not show apparent glomerular injury or tubulointerstitial fibrosis but did exhibit severe albuminuria (72.6 +/- 5.9 mg/day) and accumulation of cytoplasmic granules containing albumin in podocytes. Otsuka Long-Evans Tokushima Fatty rats also showed higher systolic blood pressure, postprandial blood glucose, collagen gene expression, desmin staining (a marker of podocyte injury) and angiotensin II levels than Long-Evans Tokushima Otsuka rats. Treatment with telmisartan did not affect postprandial blood glucose but decreased systolic blood pressure, collagen gene expression, desmin staining and angiotensin II levels. Telmisartan also prevented the development of albuminuria (0.6 +/- 0.1 mg/day at 35 weeks old) and accumulation of cytoplasmic granules. Hydralazine treatment resulted in a similar reduction in systolic blood pressure and partially attenuated the albuminuria (35.4 +/- 1.8 mg/day at 35 weeks old) but did not affect the other parameters. CONCLUSION: The present results suggest the contribution of augmented intrarenal angiotensin II levels to the initiation and progression of albuminuria as well as podocyte abnormalities in type 2 diabetic rats. Angiotensin II blockade may inhibit the transition from microalbuminuria to overt nephropathy through prevention of intrarenal angiotensin II augmentation, independently of changes in blood pressure and glucose levels.


Assuntos
Albuminúria/tratamento farmacológico , Angiotensina II/metabolismo , Inibidores da Enzima Conversora de Angiotensina/farmacologia , Benzimidazóis/farmacologia , Benzoatos/farmacologia , Diabetes Mellitus Tipo 2/tratamento farmacológico , Albuminúria/metabolismo , Albuminúria/patologia , Animais , Glicemia/efeitos dos fármacos , Pressão Sanguínea/efeitos dos fármacos , Colágeno/genética , Colágeno/metabolismo , Diabetes Mellitus Tipo 2/metabolismo , Diabetes Mellitus Tipo 2/patologia , Nefropatias Diabéticas/tratamento farmacológico , Nefropatias Diabéticas/metabolismo , Nefropatias Diabéticas/patologia , Hipertensão Renal/tratamento farmacológico , Hipertensão Renal/metabolismo , Hipertensão Renal/patologia , Córtex Renal/efeitos dos fármacos , Córtex Renal/metabolismo , Córtex Renal/patologia , Masculino , Podócitos/efeitos dos fármacos , Podócitos/metabolismo , Podócitos/patologia , RNA Mensageiro/metabolismo , Ratos , Ratos Endogâmicos OLETF , Telmisartan
12.
Microsc Res Tech ; 81(11): 1318-1324, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30295362

RESUMO

To investigate the correlation between mineral formation and enhanced expressions of some proteins using undecalcified frozen bone sections. Histological studies have revealed that some proteins, such as BMP2, BMPR1A, and Connexin 43, are expressed in and around sites of ectopic ossification. However, the relationship between the expressed proteins considered to be associated with the ossification and mineral formation in vivo is not clear. Ectonucleotide pyrophosphatase phosphodiesterase 1 (ENPP1)-mutant spinal hyperostotic TWY mice and ICR mice as controls were euthanized after calcein labeling, and undecalcified frozen sections were obtained from the middle thoracic spine. Intervertebral disc areas were examined histologically and by measuring calcein-labeled areas and areas showing immunoreactivity for BMP2, BMPR1A, and Connexin 43. Calcein-labeled areas, indicating mineralization in the ectopic mineralization sites, were significantly larger in the mutant mice than in controls. The expression of Connexin 43 was elevated in the annulus fibrosus. Increases in the calcein-labeled areas was not correlated with increases in the areas showing immunoreactivity for Connexin 43 in the annulus fibrosus. There was no statistical correlation between enhanced immunohistochemical expression and elevated calcein-labeled areas. By applying the morphometrical analysis method using undecalcified frozen sections to ENPP1-mutant mice, quantitative evaluation of the mineralization and proteins expressed in the surrounding area in the same animal became possible.


Assuntos
Proteína Morfogenética Óssea 2/metabolismo , Receptores de Proteínas Morfogenéticas Ósseas Tipo I/metabolismo , Conexina 43/metabolismo , Disco Intervertebral/patologia , Ossificação do Ligamento Longitudinal Posterior/patologia , Diester Fosfórico Hidrolases/genética , Pirofosfatases/genética , Animais , Anel Fibroso/metabolismo , Modelos Animais de Doenças , Fluoresceínas/metabolismo , Secções Congeladas , Masculino , Camundongos , Camundongos Endogâmicos ICR
13.
Microsc Res Tech ; 69(1): 29-35, 2006 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-16416408

RESUMO

We examined age-related changes in the blood-brain barrier (BBB) of neural cell-specific hypoxia inducible factor-1alpha (HIF-1alpha) deficient mice, which showed hydrocephalus with neuronal cell loss, to investigate an effect of neural cell-specific HIF-1alpha deficiency or hydrocephalus on vascular function. Vascular permeability of horseradish peroxidase (HRP) and binding of cationized ferritin (CF) particles to the endothelial cell luminal surface, as a marker of glycocalyx, were investigated. The thickness of CF-labeled glycocalyx was significantly decreased in the cortex in mutant mice compared with that of control mice, although it was not paralleled by increased vascular permeability. In addition, strong staining for HRP was seen around vessels located along the hippocampal fissure in 24-month-old mutant mice. The reaction product of HRP appeared in an increasing number of the endothelial cell abluminal vesicles and within the thickened basal lamina of arterioles in the hippocampus, showing increased vascular permeability. There were no leaky vessels in 10-week-old mutant mice or 10-week-old and 24-month-old control mice. These findings suggest the necessity of two factors, aging and hydrocephalus, for BBB dysfunction in HIF-1alpha deficient mice.


Assuntos
Envelhecimento , Barreira Hematoencefálica/fisiologia , Permeabilidade Capilar , Hipocampo/irrigação sanguínea , Subunidade alfa do Fator 1 Induzível por Hipóxia/deficiência , Animais , Membrana Basal/ultraestrutura , Vasos Sanguíneos/fisiologia , Vasos Sanguíneos/ultraestrutura , Células Endoteliais/citologia , Ferritinas/metabolismo , Glicocálix , Peroxidase do Rábano Silvestre , Hidrocefalia/patologia , Camundongos , Microscopia , Microscopia Eletrônica de Transmissão , Vesículas Transportadoras/ultraestrutura
14.
Microsc Res Tech ; 79(9): 833-7, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27333535

RESUMO

Their aim was to examine whether microvascular leakage of endogenous albumin, a representative marker for blood-brain barrier (BBB) damage, was induced in the periventricular area of diabetic db/db mice because periventricular white matter hyperintensity formation in magnetic resonance images was accelerating in elderly patients with diabetes mellitus. Using light and electron microscopes, and semi-quantitative analysis techniques, immunoreactivity of endogenous albumin, indicating vascular permeability, was examined in the periventricular area and spinal cord of db/db mice and db/+m control mice. Greater immunoreactivity of albumin was observed in the vessel wall of the periventricular area of db/db mice than in controls. Additionally, weak immunoreactivity was observed in the spinal cord of both db/db mice and controls. The number of gold particles, indicating immunoreactivity of albumin, in the perivascular area of db/db mice was significantly higher than that of control mice, but there was no significant difference in the number of particles in the spinal cord between db/db mice and controls. These findings suggest that albumin microvascular leakage, or BBB breakdown, is induced in the periventricular area of diabetic mice. Microsc. Res. Tech. 79:833-837, 2016. © 2016 Wiley Periodicals, Inc.


Assuntos
Albuminas/análise , Barreira Hematoencefálica/diagnóstico por imagem , Barreira Hematoencefálica/ultraestrutura , Encéfalo/diagnóstico por imagem , Encéfalo/ultraestrutura , Diabetes Mellitus Experimental/diagnóstico por imagem , Albuminas/química , Animais , Química Encefálica , Imuno-Histoquímica , Masculino , Camundongos , Camundongos Transgênicos , Microscopia Imunoeletrônica
15.
J Hypertens ; 23(10): 1887-94, 2005 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-16148613

RESUMO

OBJECTIVE: D-allose, an all-cis aldo-hexose, is non-caloric and possesses antioxidant properties. We investigated the effects of oral D-allose supplementation on the development of high blood pressure and the oxidative status in two genetically hypertensive animal models: Dahl salt-sensitive hypertensive (DS) rats and spontaneously hypertensive rats. METHODS AND RESULTS: The systolic blood pressure of DS rats fed a 4% salt diet for 4 weeks significantly increased from 122+/-8 to 161+/-5 mmHg as compared with DS rats fed a normal salt diet (138+/-5 mmHg at 4 weeks), whereas concordant supplementation of D-allose, but not D-glucose, with a dose of 2 g/kg daily to salt-loaded DS rats suppressed the development of high blood pressure (135+/-7 mmHg at 4 weeks), accompanied with decreases in superoxide production in the aorta that was determined by the lucigenin chemiluminescence and dihydroethidium staining. The increases of urinary protein secretion of salt-loaded DS rats were prevented by D-allose supplementation (DS rats fed 0.5% salt, 18.2+/-6.3 mg/day; DS rats fed 4% salt alone, 81.8+/-16.5 mg/day; DS rats fed 4% salt+D-allose, 31.3+/-11.8 mg/day; DS rats fed 4% salt+D-glucose, 85.3+/-20.5 mg/day). On the other hand, D-allose supplementation in spontaneously hypertensive rats had no significant effect on the blood pressure or the aortic superoxide production during the early developing stage of hypertension. CONCLUSIONS: These results underscore the role of enhanced oxidative stress in the pathogenesis of high blood pressure development in DS rats, and suggest the possibility of D-allose supplementation for prevention of salt-sensitive hypertension.


Assuntos
Glucose/uso terapêutico , Hipertensão/prevenção & controle , Cloreto de Sódio na Dieta/administração & dosagem , Animais , Aorta/efeitos dos fármacos , Aorta/metabolismo , Pressão Sanguínea/efeitos dos fármacos , Peso Corporal/efeitos dos fármacos , Suplementos Nutricionais , Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos , Glucose/administração & dosagem , Hipertensão/etiologia , Hipertensão/fisiopatologia , Rim/patologia , Córtex Renal/metabolismo , Masculino , Proteína Quinase 1 Ativada por Mitógeno/metabolismo , Proteína Quinase 3 Ativada por Mitógeno/metabolismo , Miocárdio/patologia , NADPH Oxidases/genética , Tamanho do Órgão/efeitos dos fármacos , Fosforilação/efeitos dos fármacos , Proteinúria/prevenção & controle , Proteinúria/urina , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Ratos , Ratos Endogâmicos Dahl , Ratos Endogâmicos SHR , Superóxidos/metabolismo
16.
Epilepsy Res ; 61(1-3): 141-51, 2004.
Artigo em Inglês | MEDLINE | ID: mdl-15451015

RESUMO

The classical view of the function of radial glia in brain development is a supporting function guiding radial neural migration. However, recent evidence indicates that they may play key roles in neurogenesis and gliogenesis, as ubiquitous precursors that generate neurons and glia. Although we previously reported the emergence of radial glia after spinal cord injury in adult rats, their precise function in this process is still unknown. In the present study, we examined emergence of radial glia in rat brain during progression of kindling, by performing immunohistochemical staining for vimentin which is a specific marker of radial glia. Vimentin immunoreactivity was found to be highest at clonus 3 and then decreased at clonus 5 in the hippocampal formation, regions around the third ventricle, caudate putamen and lateral habenular nucleus. Contrast, vimentin immunoreactivity consistently increased with progression of kindling in the cingulum and parietal cortex. These findings indicate dynamic changes in vimentin expression dependent on the kindling stage of seizure-prone state, and suggest that these changes play roles in formation of new circuits following kindling.


Assuntos
Química Encefálica/fisiologia , Excitação Neurológica/fisiologia , Neuroglia/metabolismo , Vimentina/biossíntese , Animais , Biomarcadores , Hipocampo/metabolismo , Hipocampo/patologia , Imuno-Histoquímica , Masculino , Lobo Parietal/metabolismo , Células Piramidais/metabolismo , Ratos , Ratos Sprague-Dawley , Terceiro Ventrículo/metabolismo
17.
Can J Neurol Sci ; 31(4): 514-9, 2004 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-15595259

RESUMO

BACKGROUND: Nestin is a class VI intermediate filament protein, expressed during early embryonic development in mammals. Postnatally, nestin and its mRNA are down-regulated and gradually disappear. Recently, nestin expression has been detected in the adult nervous system, and it has been suggested that this protein may be related to neurogenesis, although, its role in the mechanism of neurogenesis is not known. METHODS: The present study examined the localization of nestin in CNS tissue of the amygdaloid kindled rat by light and electron microscopy. RESULTS: Kindled animals showed nestin expression mainly in the piriform cortex and the perirhinal cortex. By light microscopy, nestin was shown to be expressed in astrocytes, neurons, and endothelial cells. Electron microscopy demonstrated nestin expression in endothelial cells, astrocytic perivascular end feet, the rare pericyte, neurons and oligodendrocytes. CONCLUSION: We conclude that epilepsy causes widespread nestin expression in many cell types in the CNS, including non-neural cells.


Assuntos
Tonsila do Cerebelo/metabolismo , Proteínas de Filamentos Intermediários/metabolismo , Excitação Neurológica/metabolismo , Proteínas do Tecido Nervoso/metabolismo , Giro Para-Hipocampal/metabolismo , Lobo Temporal/metabolismo , Tonsila do Cerebelo/citologia , Animais , Astrócitos/metabolismo , Estimulação Elétrica , Células Endoteliais/metabolismo , Proteína Glial Fibrilar Ácida/metabolismo , Imuno-Histoquímica , Masculino , Nestina , Giro Para-Hipocampal/citologia , Ratos , Ratos Sprague-Dawley , Lobo Temporal/citologia , Distribuição Tecidual
18.
Curr Med Chem ; 21(35): 4085-90, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25312211

RESUMO

Intravascular substances invade extracellular spaces in the brain via endothelial cells in the sites without bloodbrain barrier (BBB) and move not only in the cerebrospinal fluid (CSF) but also in the interstitial fluid (ISF) of brain parenchyma adjacent to non-BBB sites. It is likely that CSF drains directly into the blood via arachnoid villi and granulations and also to lymph nodes via subarachnoid spaces in the brain and nasal lymphatics, whereas ISF drains to cervical lymph nodes through pathways along vascular wall of capillaries and arteries. As the supposed pathways of fluids seem to be critical for the maintenance of normal brain function, it is reasonable to suspect that an obstacle to the passage of fluids through these pathways likely induces some kinds of brain dysfunction such as Alzheimer's disease. According to assumed pathways for the elimination of amyloid-ß (Aß) from the brain, Aß peptides produced mainly in neurons are degraded by peptidases, flow out of the brain parenchyma into the blood through efflux transporters located in cerebral vessels, drain through perivascular pathways into the cervical lymph nodes, or are taken up by some kinds of cells in the brain. As for the perivascular pathways, ISF including Aß peptides diffuses in the extracellular spaces of the brain parenchyma, enters basement membranes of capillaries, passes into the tunica media of arteries, and drains out of the brain. In this review, these pathways for the clearance of fluids including Aß from the brain into the blood are briefly reviewed and the relationship between dysfunction of these pathways and brain diseases is discussed.


Assuntos
Peptídeos beta-Amiloides/metabolismo , Encéfalo/metabolismo , Doença de Alzheimer/metabolismo , Doença de Alzheimer/patologia , Peptídeos beta-Amiloides/líquido cefalorraquidiano , Barreira Hematoencefálica/metabolismo , Líquido Extracelular/metabolismo , Humanos , Proteínas de Membrana Transportadoras/metabolismo , Peptídeo Hidrolases/metabolismo
19.
Arch Gerontol Geriatr ; 56(1): 75-9, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-22884668

RESUMO

SAMP8, senescence accelerated mice with age-related deficits in memory and learning, are known to show age-related increases of amyloid precursor protein (APP) and immunopositivity for amyloid-ß (Aß) proteins, and moreover to be under elevated oxidative stress. The elevated expression of class B scavenger receptor CD36, which is the receptor of oxidized LDL and also one of efflux transporters of Aß proteins in the cerebral vessels, is thought to mediate free radical production in cerebral ischemia and induce oxidative stress. Accordingly, by real-time quantitative reverse transcriptase-polymerase chain reaction (RT-PCR), Western blotting, and immunohistochemical techniques, we examined whether the expression of CD36 was increased in the brains of 10-12-week-old SAMP8 with elevated oxidative stress. Ten to 12-week-old SAMR1 mice were used as controls without the features. The gene and protein expression of CD36 was significantly higher in the brains of SAMP8 than those of SAMR1. Confocal microscopic examination revealed that the CD36 immunoreactivity was seen in the cytoplasm of endothelial cells and F4/80-positive perivascular cells of the brains. These findings indicate that the expression of CD36 in the brains of SAMP8 is increased compared with that of SAMR1.


Assuntos
Envelhecimento/metabolismo , Encéfalo/metabolismo , Antígenos CD36/metabolismo , Animais , Western Blotting , Antígenos CD36/biossíntese , Masculino , Camundongos , Camundongos Endogâmicos , Microscopia Confocal , Estresse Oxidativo , Reação em Cadeia da Polimerase em Tempo Real , Reação em Cadeia da Polimerase Via Transcriptase Reversa
20.
Neurosci Lett ; 513(1): 47-50, 2012 Mar 28.
Artigo em Inglês | MEDLINE | ID: mdl-22343024

RESUMO

A homeostasis of the electrochemical properties and volume of the endolymph in the inner ear is essential for hearing and equilibrium sensing and is maintained by ion-transport across an epithelial tissue, the endolymphatic sac. One of the key proteins in the maintenance is Na(+), K(+)-ATPase. Although we previously found that the Na(+), K(+)-ATPase in the sac plays a pivotal role in the control of the endolymphatic volume, the mechanism remains unclear. Therefore, in this study, we examined the expression of FXYD6, a functional modulator of the Na(+), K(+)-ATPase, in the epithelial cells of the endolymphatic sac using various approaches. Laser capture microdissection RT-PCR was used to identify FXYD6 mRNA in the endolymphatic sac. Immunolabeling with the specific antibody showed that FXYD6 was predominantly expressed in the intermediate portion of the endolymphatic sac, and it was colocalized with the Na(+), K(+)-ATPase. Because the Na(+), K(+)-ATPase in this region is known to exhibit a high level of activity, an interaction of FXYD6 with this transporter may be critically involved in the regulation of the characteristics of the endolymph.


Assuntos
Saco Endolinfático/metabolismo , Canais Iônicos/metabolismo , Animais , Química Encefálica , Cóclea/metabolismo , Eletroforese em Gel de Ágar , Epitélio/metabolismo , Cobaias , Imuno-Histoquímica , Microdissecção e Captura a Laser , RNA/biossíntese , RNA/isolamento & purificação , RNA Mensageiro/biossíntese , RNA Mensageiro/genética , Reação em Cadeia da Polimerase em Tempo Real , ATPase Trocadora de Sódio-Potássio/metabolismo , Fixação de Tecidos
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