RESUMO
The existence of enhanced humoral immunity against sperm antigens in patients with ulcerative colitis might be a result of the increased intestinal permeability, and may be associated with immunization against antigens of the common intestinal flora possessing common antigenicity with spermatozoa. The result of these two processes is statistically significant increased antisperm antibody (ASA) incidence in patients with ulcerative colitis compared to the healthy blood donors of proven fertility without clinical symptoms of inflammatory bowel diseases at the same age.
Assuntos
Autoanticorpos/sangue , Colite Ulcerativa/imunologia , Espermatozoides/imunologia , Adolescente , Adulto , Croácia , Feminino , Humanos , Masculino , Pessoa de Meia-IdadeRESUMO
PROBLEM: Establishing the correlation between antichlamydial antibodies (AchAbs) and antisperm antibodies (ASA) in patients with chlamydial infections. METHOD OF STUDY: ASA were studied in sera from patients (142 with genital, 57 with ocular chlamydial infections) and control group (n = 100) by gelatin and tray agglutination test (TAT), sperm immobilization test (SIT) and ELISA. AchAbs were revealed by ELISA. RESULTS: A significantly higher (P < 0.05) ASA incidence was noted in patients with genital infections as compared with controls and patients with ophthalmologic infection (P < 0.0001), but not between patients with ophthalmologic infection and controls (P > 0.05). A significant correlation was established between AchAbs and ASA for TAT (r = 0.8214, P = 0.0341), SIT (r = 0.797, P = 0.032) and ELISA (r = 0.8519, P = 0.0313) in patients with genital infections only. CONCLUSIONS: The genital Chlamydia infection may play a role in the induction of ASA. This is probably a result of the inflammatory process, but not of cross-reactivity between sperm and Chlamydia trachomatis antigens.
Assuntos
Anticorpos Antibacterianos/análise , Autoanticorpos/análise , Infecções por Chlamydia/imunologia , Chlamydia trachomatis/isolamento & purificação , Espermatozoides/imunologia , Adulto , Testes de Aglutinação , Anticorpos , Bulgária/epidemiologia , Estudos de Casos e Controles , Infecções por Chlamydia/sangue , Infecções por Chlamydia/epidemiologia , Chlamydia trachomatis/imunologia , Conjuntivite de Inclusão/sangue , Conjuntivite de Inclusão/imunologia , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Infertilidade/sangue , Infertilidade/etiologia , Infertilidade/imunologia , Masculino , Pessoa de Meia-Idade , Motilidade dos EspermatozoidesRESUMO
PROBLEM: Establishing the age-dependent patterns of sperm antibody levels among normal humans. METHODS OF STUDY: Sera samples from 498 healthy subjects aged 0-97 years - 246 males and 252 females - were tested by the gelatin agglutination test of Kibrick, tray agglutination test of Friberg, sperm immobilization test of Isojima and enzyme-linked immunosorbent assay (ELISA). RESULTS: We found a significant increase in the level of sperm agglutinins after 40 years, which decreased after 88 years. The antibodies detected by ELISA were the highest among prepubertal subjects and also declined with aging. No age-dependent changes were established for the sperm immobilizins. With few exceptions, there were no significant differences between male and female sera, as well as between sera of newborn and their mothers. CONCLUSIONS: These data are similar to those established for the age-dependent changes of antibodies towards exogenous antigens, suggesting that the 'naturally occurring' antibodies against human spermatozoa are not auto-/isoantibodies.