Cell-intrinsic lysosomal lipolysis is essential for alternative activation of macrophages.

Alternative (M2) activation of macrophages driven via the α-chain of the receptor for interleukin 4 (IL-4Rα) is important for immunity to parasites, wound healing, the prevention of atherosclerosis and metabolic homeostasis. M2 polarization is dependent on fatty acid oxidation (FAO), but the source of the fatty acids that support this metabolic program has not been clear. We found that the uptake of triacylglycerol substrates via the scavenger receptor CD36 and their subsequent lipolysis by lysosomal acid lipase (LAL) was important for the engagement of elevated oxidative phosphorylation, enhanced spare respiratory capacity (SRC), prolonged survival and expression of genes that together define M2 activation. Inhibition of lipolysis suppressed M2 activation during infection with a parasitic helminth and blocked protective responses to this pathogen. Our findings delineate a critical role for cell-intrinsic lysosomal lipolysis in M2 activation.

Metabolic reprogramming of the myeloid lineage by Schistosoma mansoni infection persists independently of antigen exposure.

Macrophages have a defined role in the pathogenesis of metabolic disease and cholesterol metabolism where alternative activation of macrophages is thought to be beneficial to both glucose and cholesterol metabolism during high fat diet induced disease. It is well established that helminth infection protects from metabolic disease, but the mechanisms underlying protection are not well understood. Here, we investigated the effects of Schistosoma mansoni infection and cytokine activation in the metabolic signatures of bone marrow derived macrophages using an approach that integrated transcriptomics, metabolomics, and lipidomics in a metabolic disease prone mouse model. We demonstrate that bone marrow derived macrophages (BMDM) from S. mansoni infected male ApoE-/- mice have dramatically increased mitochondrial respiration compared to those from uninfected mice. This change is associated with increased glucose and palmitate shuttling into TCA cycle intermediates, increased accumulation of free fatty acids, and decreased accumulation of cellular cholesterol esters, tri and diglycerides, and is dependent on mgll activity. Systemic injection of IL-4 complexes is unable to recapitulate either reductions in systemic glucose AUC or the re-programing of BMDM mitochondrial respiration seen in infected males. Importantly, the metabolic reprogramming of male myeloid cells is transferrable via bone marrow transplantation to an uninfected host, indicating maintenance of reprogramming in the absence of sustained antigen exposure. Finally, schistosome induced metabolic and bone marrow modulation is sex-dependent, with infection protecting male, but not female mice from glucose intolerance and obesity. Our findings identify a transferable, long-lasting sex-dependent reprograming of the metabolic signature of macrophages by helminth infection, providing key mechanistic insight into the factors regulating the beneficial roles of helminth infection in metabolic disease.

Noninvasive Diagnostic for COVID-19 from Saliva Biofluid via FTIR Spectroscopy and Multivariate Analysis.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused the worst global health crisis in living memory. The reverse transcription polymerase chain reaction (RT-qPCR) is considered the gold standard diagnostic method, but it exhibits limitations in the face of enormous demands. We evaluated a mid-infrared (MIR) data set of 237 saliva samples obtained from symptomatic patients (138 COVID-19 infections diagnosed via RT-qPCR). MIR spectra were evaluated via unsupervised random forest (URF) and classification models. Linear discriminant analysis (LDA) was applied following the genetic algorithm (GA-LDA), successive projection algorithm (SPA-LDA), partial least squares (PLS-DA), and a combination of dimension reduction and variable selection methods by particle swarm optimization (PSO-PLS-DA). Additionally, a consensus class was used. URF models can identify structures even in highly complex data. Individual models performed well, but the consensus class improved the validation performance to 85% accuracy, 93% sensitivity, 83% specificity, and a Matthew's correlation coefficient value of 0.69, with information at different spectral regions. Therefore, through this unsupervised and supervised framework methodology, it is possible to better highlight the spectral regions associated with positive samples, including lipid (∼1700 cm-1), protein (∼1400 cm-1), and nucleic acid (∼1200-950 cm-1) regions. This methodology presents an important tool for a fast, noninvasive diagnostic technique, reducing costs and allowing for risk reduction strategies.

Acute and repeated dose 28-day oral toxicity of Chrysobalanusicaco L. leaf aqueous extract.

Chrysobalanus icaco L. is a native plant of Brazil used as a food source and traditionally for the treatment of various diseases. The aim of study was performed the phytochemical analysis by UPLC-DAD-ESI-QTOF-MS/MS, and evaluated acute and repeated dose oral toxicities of the C. icaco L. leaf aqueous extract (AECi). The acute toxicity study was performed using a dose of AECi 2000 mg/kg, while the repeated dose toxicity study, the AECi was administered daily at doses of 100, 200 and 400 mg/kg, for 28 days. Behavior and mortality of animals were observed during the test period and body weight, as well water and eating consumption. Hematological, biochemical parameters and histopathological examinations were carried out. Phytochemical analysis of the AECi revealed the presence of flavonoids and tannins. Oral single dose of 2000 mg/kg of AECi resulted in no mortalities or abnormal clinical signs. Studies of repeated dose toxicity promoted a reduction in the body weight of treated animals and an increase of hepatic enzymes alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in both, males and females. Histopathological analyzes showed alterations in the livers of animals treated with AECi. Thus, this study recommends the population take care when using this species, especially during prolonged periods.

Ly6Chi monocyte recruitment is responsible for Th2 associated host-protective macrophage accumulation in liver inflammation due to schistosomiasis.

Accumulation of M2 macrophages in the liver, within the context of a strong Th2 response, is a hallmark of infection with the parasitic helminth, Schistosoma mansoni, but the origin of these cells is unclear. To explore this, we examined the relatedness of macrophages to monocytes in this setting. Our data show that both monocyte-derived and resident macrophages are engaged in the response to infection. Infection caused CCR2-dependent increases in numbers of Ly6Chi monocytes in blood and liver and of CX3CR1+ macrophages in diseased liver. Ly6Chi monocytes recovered from liver had the potential to differentiate into macrophages when cultured with M-CSF. Using pulse chase BrdU labeling, we found that most hepatic macrophages in infected mice arose from monocytes. Consistent with this, deletion of monocytes led to the loss of a subpopulation of hepatic CD11chi macrophages that was present in infected but not naïve mice. This was accompanied by a reduction in the size of egg-associated granulomas and significantly exacerbated disease. In addition to the involvement of monocytes and monocyte-derived macrophages in hepatic inflammation due to infection, we observed increased incorporation of BrdU and expression of Ki67 and MHC II in resident macrophages, indicating that these cells are participating in the response. Expression of both M2 and M1 marker genes was increased in liver from infected vs. naive mice. The M2 fingerprint in the liver was not accounted for by a single cell type, but rather reflected expression of M2 genes by various cells including macrophages, neutrophils, eosinophils and monocytes. Our data point to monocyte recruitment as the dominant process for increasing macrophage cell numbers in the liver during schistosomiasis.

Extract of Bowdichia virgilioides and maackiain as larvicidal agent against Aedes aegypti mosquito.

The larvicidal activities of extracts of three hardwood species (Hymenaea stigonorcapa, Anadenanthera colubrina and Bowdichia virgilioides) against 4th instar larvae of Aedes aegypti were evaluated using WHO guidelines. Extracts of H. stignocarpa and A. colubrina showed weak activity. The highest larvicidal effect was obtained with the cyclohexane extract of the heartwood of B. virgilioides, which caused 100% mortality at concentrations at 50 and 100 µg/mL. Fraction toluene/EtOAc (8:2) from this extract showed larvicidal activity (LC50 = 34.90 ± 1.27 µg/mL). A mixture of two compounds identified as medicarpin and maackiain exhibited a very good larvicidal activity (sub-fraction 2, LC50 = 17.5 ± 1.87 µg/mL) and maackiain showed to be a strong larvicidal compound (LC50 = 21.95 ± 1.34 µg/mL). This result can be of value in the search for new natural larvicidal compounds from other hardwood plant extracts and presents the first report of B. virgilioides being used to control a mosquito vector.

Fast screening using attenuated total reflectance- fourier transform infrared (ATR-FTIR) spectroscopy of patients based on D-dimer threshold value.

Attenuated Total Reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy is an emerging technology in the medical field. Blood D-dimer was initially studied as a marker of the activation of coagulation and fibrinolysis. It is mainly used as a potential diagnosis screening test for pulmonary embolism or deep vein thrombosis but was recently associated with COVID-19 severity. This study aimed to evaluate the use of ATR-FTIR spectroscopy with machine learning to classify plasma D-dimer concentrations. The plasma ATR-FTIR spectra from 100 patients were studied through principal component analysis (PCA) and two supervised approaches: genetic algorithm with linear discriminant analysis (GA-LDA) and partial least squares with linear discriminant (PLS-DA). The spectra were truncated to the fingerprint region (1800-1000 cm-1). The GA-LDA method effectively classified patients according to D-dimer cutoff (≤0.5 µg/mL and >0.5 µg/mL) with 87.5 % specificity and 100 % sensitivity on the training set, and 85.7 % specificity, and 95.6 % sensitivity on the test set. Thus, we demonstrate that ATR-FTIR spectroscopy might be an important additional tool for classifying patients according to D-dimer values. ATR-FTIR spectral analyses associated with clinical evidence can contribute to a faster and more accurate medical diagnosis, reduce patient morbidity, and save resources and demand for professionals.

A one-class classification approach for authentication of specialty coffees by inductively coupled plasma mass spectroscopy (ICP-MS).

Due to the lucrative nature of specialty coffees, there have been instances of adulteration where low-cost materials are mixed in to increase the overall volume, resulting in illegal profit. A widely used and recommended approach to detect possible adulteration is the application of one-class classifiers (OCC), which only require information about the target class to build the models. Thus, this work aimed to identify adulterations in specialty coffees with low-quality coffee using multielement analysis determined by ICP-MS and to evaluate the performance of one-class classifiers (dd-SIMCA, OCRF, and OCPLS). Therefore, authentic specialty coffee samples were adulterated with low-quality coffee in 25 % to 75 % (w/w) proportions. Samples were subjected to acid decomposition for analysis by ICP-MS. OCPLS method presented the best performance to detect adulterations with low-quality coffee in specialty coffees, showing higher specificity (SPE = 100 %) and reliability rate (RLR = 94.3 %).

Correlation analysis of modern analytical data - a chemometric dissection of spectral and chromatographic variables.

The Standard Practices for Infrared Multivariate Quantitative Analysis (ASTM E1655) provide a guide for determining physicochemical properties of materials using multivariate calibration techniques applied to chemical sources that have high multicollinearity and correlated information. Partial least squares (PLS) is the most widely used multivariate regression method due to its excellent prediction capabilities and easy optimization. Initially applied to chromatographic data, PLS has also shown great results in near-infrared (NIR) and mid-infrared (MIR) spectroscopies. However, complex chemical matrices with low correlation may not be efficiently modeled using PLS or other multivariate analyses limited by grouping similar information (such as latent variables or principal components). Therefore, this study aims to evaluate the multicollinearity of different analytical techniques, such as high-temperature gas chromatography (HTGC), NIR, MIR, hydrogen nuclear magnetic resonance (1H NMR), carbon-13 nuclear magnetic resonance (13C NMR), and Fourier transform ion cyclotron resonance mass spectrometry coupled to the electrospray source in positive and negative ionization modes (ESI(±)FT-ICR). Descriptive statistics (coefficient of determination, R2) and principal component analysis (PCA) were used to identify the distribution of correlated information. Results showed that NIR and MIR spectroscopies exhibited a higher percentage of correlated variables, while 13C NMR and ESI(±)FT-ICR MS had more discrete profiles. Therefore, PLS development may be more effectively applied to NIR, MIR, and 1H NMR data, while 13C NMR and mass spectra may require other algorithms or variable selection methods in combination with PLS.

Immunological and viral features in patients with overactive bladder associated with human T-cell lymphotropic virus type 1 infection.

The majority of patients infected with human T-cell lymphotropic virus-type 1 (HTLV-1) are considered carriers, but a high frequency of urinary symptoms of overactive bladder, common in HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP) have been documented in these patients. The aim of this study was to determine if immunological and viral factors that are seen in HAM/TSP are also observed in these patients. Participants were classified as HTLV-1 carriers (n = 45), HTLV-1 patients suffering from overactive bladder (n = 45) and HAM/TSP (n = 45). Cells from HTLV-1 overactive bladder patients produced spontaneously more proinflammatory cytokines than carriers. TNF-α and IL-17 levels were similar in HAM/TSP and HTLV-1 overactive bladder patients. High proviral load was found in patients with overactive bladder and HAM/TSP and correlated with proinflammatory cytokines. In contrast with findings in patients with HAM/TSP, serum levels of Th1 chemokines were similar in HTLV-1 overactive bladder and carriers. Exogenous addition of regulatory cytokines decreased spontaneous IFN-γ production in cell cultures from HTLV-1 overactive bladder patients. The results show that HTLV-1 overactive bladder and HAM/TSP patients have in common some immunological features as well as similar proviral load profile. The data show that HTLV-1 overactive bladder patients are still able to down regulate their inflammatory immune response. In addition, these patients express levels of chemokines similar to carriers, which may explain why they have yet to develop the same degree of spinal cord damage as seen in patients with HAM/TSP. These patients present symptoms of overactive bladder, which may be an early sign of HAM/TSP.

Portable Raman spectroscopy applied to the study of drugs of abuse.

The use of drugs of abuse has grown significantly in recent decades. In forensic chemistry, methods of identifying and characterizing illicit drugs contribute to the interests of researchers, experts, and public security authorities. Among existing methods, portable Raman spectroscopy is notable for performing rapid, non-destructive, and highly selective analysis in the laboratory or on-site. When the resulting spectral data are paired with chemometric tools, methods of exploratory analysis and multivariate calibration can be developed. Thus, this work describes the application of Raman spectroscopy associated with principal component analysis (PCA) and interval principal component analysis (iPCA) to assessing trends in samples of cocaine (n = 40), crack (n = 33), and their main adulterants (n = 5) and diluents (n = 5), tablets of ecstasy (n = 14), designer drugs papers (n = 27), and alcoholic solutions adulterated with benzodiazepines (alprazolam and diazepam). In addition, competitive adaptive reweighted sampling (CARS) combined with partial least squares (PLS) regression (CARSPLS) was used to quantify adulterants (benzocaine, lidocaine, and procaine) in binary mixtures with crack (n = 21) and solutions of cachaça adulterated with bromazepam (n = 11).

Evaluation of cytotoxic, immunomodulatory effects, antimicrobial activities and phytochemical constituents from various extracts of Passiflora edulis F. flavicarpa (Passifloraceae).

The leaves of P. edulis were subjected to physicochemical analysis, such as ion content, extractives, and structural molecules. The hexanic, ethanolic and ethyl acetate extracts were submitted to phytochemical analyzes by GC-MS, HPLC-MS, and spectrophotometry. In addition, antioxidant (DPPH, ABTS and TAA methods) potential, antimicrobial (MIC method) action, cytotoxicity and immunostimulant activity (flow cytometry analysis) were performed. The extracts showed a moderate antioxidant capacity and revealed the presence of several metabolites, mainly phenols, such as caffeic acid, p-coumaric acid and luteolin. The ethyl acetate and ethanolic extracts showed antifungal activity. In addition, the extracts did not affect splenocytes viability at 12.5 µg/mL and promoted the production of IL-6, IL-10, IL-17 and TNF-α cytokines. P. edulis extracts showed antifungal and antioxidant activity and were able to induce immunostimulatory action in splenocyte cultures in vitro.

[Changing in process that involves rest scales of nursing assistant]. / Mudança no processo que envolve escalas de folgas de auxiliares de enfermagem.

Describe the implantation of a new process of management of the nursing assistant rest scale of a hospitalization unit of a universitarian hospital. It is presented the reality before the proposal, when the rests were distributed along the week; the reasons that took to the proposition of the new method and the working of the new scale, with rests in weekends and holidays. Among the benefits already seen include: bigger number of professionals working during the days of bigger work flow; more time of leisure of the workers with their families; bigger personal integration and professional of the group; scales organized for a year, which allows the employee plan his vacation, rests and studies; and bigger satisfaction with the work, with the new scale being considered a professional conquest.

Simplesmente mães: construção compartilhada de tecnologias sobre pré-natal de mulheres com deficiência visual / Simply mothers: shared elaboration of technologies on prenatal care of visually impaired women / Simplemente madres: creación compartida de tecnologías sobre el control prenatal para mujeres con discapacidad visual

RESUMO Objetivo: Desenvolver tecnologias educacionais sobre pré-natal com e para mulheres deficientes visuais. Método: Estudo metodológico com interface participativa e abordagem qualitativa. Realizado em uma Unidade Técnica Especializada no município de Belém, Pará, Brasil. A produção de dados ocorreu entre agosto e setembro de 2021 com seis mulheres. Utilizou-se o DOSVOX como recurso de comunicação para que as participantes respondessem quatro instrumentos com vistas ao desenvolvimento das tecnologias. A análise foi de conteúdo temático. Resultados: As mulheres com deficiência visual querem respeito à sua autonomia, inclusão e informação dos profissionais. As tecnologias produzidas apontam as demandas específicas de mulheres com deficiência visual e a importância de preservar a autonomia durante a realização do pré-natal. Conclusão: Tecnologias produzidas de forma participativa apontam perspectivas e necessidades específicas das mulheres sobre o pré-natal e poderão subsidiar tanto o agir dos enfermeiros nas consultas como favorecer mulheres com deficiência visual durante o pré-natal.

ABSTRACT Objective: To develop educational technologies on prenatal care with and for visually impaired women. Method: A methodological study with a participatory interface and qualitative approach. It was carried out at a Specialized Technical Unit in the municipality of Belém, Pará, Brazil. Data production took place between August and September 2021 with six women. DOSVOX was used as a communication resource for the participants to answer four instruments with a view to developing the technologies. The analysis was of the thematic content type. Results: Women with visual impairment want respect for their autonomy, inclusion, and information from the professionals. The technologies produced point to the specific demands of visually impaired women and to the importance of preserving autonomy during prenatal care. Conclusion: Technologies produced in a participatory way point out women's specific perspectives and needs regarding prenatal care and may support both the nurses' actions in consultations and favor women with visual impairment during prenatal care.

RESUMEN Objetivo: Desarrollar tecnologías educativas sobre el control prenatal con y para mujeres con discapacidad visual. Método: Estudio metodológico con interfaz participativa y enfoque cualitativo. Realizado en una Unidad Técnica Especializada de la ciudad de Belém, Pará, Brasil. La producción de datos se realizó entre agosto y septiembre de 2021 con seis mujeres. Se utilizó DOSVOX como recurso de comunicación para que las participantes respondieran a cuatro instrumentos a fin de desarrollar las tecnologías. El análisis fue de contenido temático. Resultados: Las mujeres con discapacidad visual quieren que se respete su autonomía, inclusión e información por parte de los profesionales. Las tecnologías creadas ponen de manifiesto las demandas específicas de las mujeres con discapacidad visual y la importancia de preservar la autonomía durante el control prenatal. Conclusión: Las tecnologías creadas de manera participativa señalan las perspectivas y necesidades específicas de las mujeres con respecto al cuidado prenatal y pueden ayudar a los enfermeros en las consultas y a las mujeres con discapacidad visual durante el control prenatal.

Anti-inflammatory and antinociceptive activities of the leaf methanol extract of Miconia minutiflora (Bonpl.) DC. and characterization of compounds by UPLC-DAD-QTOF-MS/MS.

Some species of the genus Miconia are used in Brazilian folk medicine as analgesic and anti-inflammatory; however, several species of this genus are still poorly studied. Therefore, the aims of this study were to investigate the phytochemistry characterization by UPLC-DAD-QTOF-MS/MS, acute toxicity, anti-inflammatory and antinociceptive properties of Miconia minutiflora (Bonpl.) DC. The methanol extract of M. minutiflora (Mm-MeOH) was subjected to ultra-high-performance liquid chromatography (UPLC-DAD-QTOF-MS/MS) for the identification of the main phytocompounds. The anti-inflammatory properties of the extracts were studied using several inflammation models induced by carrageenan and acetic acid-induced vascular permeability. Antinociceptive effects of Mm-MeOH were assessed in nociception induced by intraperitoneal acetic acid or subplantar formalin injection. The role of α-adrenergic, cholinergic, and opioid receptors in modulating the extract's antinociceptive activity was determined. Analyses by UPLC-DAD-QTOF-MS/MS revealed the presence of ellagic acid, gallotannin, and terpenes in the methanol extract. Mm-MeOH (100 mg/kg) reduced carrageenan-induced paw edema and vascular permeability and inhibited leukocyte migration toward the air pouch and pleural cavity. Furthermore, Mm-MeOH decreased tumor necrosis factor-α (TNF-α) and interleukin-1ß (IL-1ß) levels. Administration of Mm-MeOH reduced the number of writhes by 58.9% and increased the pain threshold in the formalin test. The anti-inflammatory action mechanism of Mm-MeOH is associated with inhibition of proinflammatory cytokines TNF-α and IL-1ß, whereas the antinociceptive actions involve peripheral and central mechanisms with participation of α2-adrenergic receptors. These effects may be attributed to the presence of polyphenolics in the extract.

Gastroprotective effect of ethyl acetate extract from Avicennia schaueriana Stapf & Leechman and underlying mechanisms.

OBJECTIVE: Avicennia schaueriana Stapf is an endemic mangrove species widely used by traditional Brazilian communities as a folk remedy for the treatment of rheumatism, ulcers, and skin wounds. The aim of the present study was to evaluate the gastroprotective potential of the ethyl acetate extract from the leaves of A. schaueriana (As-AcOEt). METHODS: Ultra-performance liquid chromatography coupled with diode-array detection and quadrupole time-of-flight mass spectrometry (UPLC-DAD-QTOF-MS/MS) was performed to identify chemical constituents of the ethyl acetate extract from the leaves ofA. schaueriana. Total phenols, flavonoids and tannins were determined and antioxidant activity was evaluated using the DPPH and ABTS methods. The acute toxicity of As-AcOEt and gastroprotective activity on HCl/ethanol-induced gastric ulcers were assessed and mechanisms of action involving the role of nitric oxide, sulfhydryl compounds, and prostaglandins were investigated. RESULTS: Terpenes, flavonoids and tannins were detected in the extract. As-AcOEt exhibited antioxidant activity, with an EC50 of 42.2 ± 4.4 µg/mL (DPPH) and 73.2% inhibition of ABTS radicals. UPLC-DAD-QTOF-MS/MS analysis identified gallic acid, gallic acid derivative, ellagic acid, myricetin pentoside, myricetin deoxyhexose, quercetin pentoside, quercetin deoxyhexose, and other compounds. Gallic acid was isolated in this species for the first time. During the acute toxicity test, no deaths or changes occurred in the variables evaluated. In the ethanol-induced ulcer model, As-AcOEt reduced the ulcerative lesion index, with 50, 100 and 200 mg/kg achieving 83.8, 88.5 and 86.9% inhibition, respectively. MPO levels decreased and the gastric mucosa of the animals treated with the extract was preserved. Pre-treatment with N-omega-nitro-l-arginine methyl ester (L-NAME; NO blocker) or carbenoxolone (CBXN; NP-SH blocker) reversed the gastroprotective effect of As-AcOEt, but this effect was not reversed with the previous administration of indomethacin. CONCLUSION: The present findings reveal that the extract from the leaves ofA. schaueriana has gastroprotective effects, suggesting the involvement of nitric oxide and nonprotein sulfhydryl compounds, but not prostaglandin. Therefore, the use of A. schaueriana in Brazilian folk medicine for the treatment of gastric disorders has a scientific basis.

Antibacterial activity of labdane diterpenoids from Stemodia foliosa.

As part of a continuing interest in exploring the chemistry of Brazilian medicinal plants, three new labdane diterpenoids, 6alpha-acetoxymanoyl oxide (1), 6alpha-malonyloxymanoyl oxide (2), and 6alpha-malonyloxy-n-butyl ester manoyl oxide (3), together with the known betulinic acid, lupeol, sitosterol, and stigmasterol, were isolated from the aerial parts of Stemodia foliosa. The structures of 1-3 were established on the basis of interpretation of spectroscopic data, including HRESIMS, and 1D and 2D NMR techniques. All compounds were tested against a bacteria panel consisting of Staphylococcus aureus, Bacillus cereus, B. subtilis, B. anthracis, Micrococcus luteus, Mycobacterium smegmatis, and M. phlei. Compound 2 showed moderate activity against these strains, with MIC values in the range 7-20 microg/mL.

Educational technology to mediate care of the "kangaroo family" in the neonatal unit.

OBJECTIVE: to validate the content of an educational technology in the form of a booklet developed to mediate care of family members of newborns hospitalized in a neonatal unit. METHOD: Methodological research with content validation. Data collected from October 2011 to February 2012; research developed in three steps: development, evaluation and adaptation. The study population was 15 judges, health professionals and other professionals. The participants answered a Likert-type questionnaire with 3 blocks of evaluative items. Data were analyzed statistically. RESULTS: validation reached the results proposed in most responses (80%); 16 items exceeded the parameter, 4 were equal to the score established and 2 were below the parameter. CONCLUSION: the content of educational technologies need to be validated. The booklet, after the evaluation, constitutes an adequate device to mediate care of the family members in the NICU.

Vídeo educativo para autocuidado de pacientes com estomia de eliminação intestinal / Educational video for self-care of patients with intestinal elimination stoma / Vídeo educativo para el autocuidado de pacientes con ostomía de eliminación intestinal

RESUMO: Objetivo: descrever a construção da tecnologia educativa para mediar o autocuidado do paciente oncológico com estomia de eliminação intestinal. Método: pesquisa metodológica, realizada em duas etapas - diagnóstico situacional e revisão integrativa da literatura, realizada no hospital referência em oncologia na cidade de Belém-Pará, Brasil. A coleta foi realizada no período de janeiro a novembro de 2021, por meio de entrevistas semi-estruturada, com 10 pacientes oncológicos estomizados e em bases de dados eletrônicos. Foram analisados na perspectiva da análise de conteúdo do tipo temática e apoiado pelo software Iramuteq. Resultados: do corpus emergiu a árvore de similitude, no qual a palavra "Bolsa" possui maior centralidade e forte conexidade com as palavras, "Cuidado", "Trocar", "Placa", "Colostomia" e "Estoma". Fundamentada nesse contexto, foi produzida uma tecnologia educacional do tipo vídeo educativo. Conclusão: a produção de tecnologia com base no contexto favorece o levantamento de evidências sociais para o autocuidado com pele periestoma.

ABSTRACT Objective: to describe the construction of an educational technology to mediate the self-care of cancer patients with intestinal elimination stoma. Method: methodological research, conducted in two stages - situational diagnosis and integrative literature review, carried out in the oncology reference hospital in the city of Belém-Pará, Brazil. The collection was carried out in the period from January to November 2021, through semi-structured interviews, with 10 oncology patients with stomas and in electronic databases. They were analyzed from the perspective of content analysis of the thematic type and supported by the Iramuteq software. Results: from the corpus a tree of similarity emerged, in which the word "Bag" has greater centrality and strong connection with the words, "Care", "Change", "Plaque", "Colostomy" and "Stoma". Based on this context, an educational video technology was produced. Conclusion: the production of context-based technology favors the collection of social evidence for self-care with the peristomal skin.

RESUMEN Objetivo: describir la construcción de una tecnología educativa para mediar en el autocuidado de pacientes con cáncer con ostomía de eliminación intestinal. Método: investigación metodológica, realizada en dos etapas - diagnóstico situacional y revisión bibliográfica integradora, llevada a cabo en el hospital oncológico de referencia de la ciudad de Belém-Pará, Brasil. La recolección se realizó en el período de enero a noviembre de 2021, a través de entrevistas semiestructuradas con 10 pacientes oncológicos estomizados y en bases de datos electrónicas. Fueron analizados desde la perspectiva del análisis de contenido de tipo temático y apoyados por el software Iramuteq. Resultados: del corpus surgió el árbol de similitud, en el que la palabra "Bolsa" tiene mayor centralidad y fuerte conexión con las palabras, "Cuidado", "Cambiar", "Placa", "Colostomía" y "Estoma". A partir de este contexto, se produjo una tecnología educativa del tipo vídeo educativo. Conclusión: la producción de tecnología basada en el contexto favorece la recopilación de pruebas sociales para el autocuidado de la piel periestomal.

Aplicativo móvel para pais de primeira viagem - cuidados ao recém-nascido: relato de experiência / Aplicación móvil para padres primerizos - cuidado del recién nacido: relato de experiencia / Mobile application for first-time parents - newborn care: an experience report

Objetivo: relatar a trajetória da produção de uma tecnologia educacional sobre cuidados ao recém-nascido para pais de primeira viagem no formato de aplicativo para dispositivo móvel. Método: relato de experiência vivenciado em janeiro a outubro de 2019, realizado no Laboratório de Tecnologias Educacionais de uma universidade pública estadual na região Norte do Brasil. Resultados: elaborou-se a revisão integrativa da literatura, obtendo-se oito artigos e o corpus foi processado no software IRAMUTEQ, que gerou cinco classes. Com base nos resultados da revisão integrativa foi desenvolvida a produção do aplicativo suprindo os requisitos de funcionalidade; usabilidade; confiabilidade; eficiência; manutenibilidade e portabilidade. Conclusão: a experiência de produção de uma tecnologia educacional baseada em evidências científicas e em um modelo de sistemas, no formato de um aplicativo móvel, propiciará ao público-alvo de pais o acesso aos conteúdos atualizados e científicos acerca do cuidado seguro ao recém-nascido.

Objective: to report the trajectory of an educational technology production on newborn care for first-time parents in the format of a mobile application. Method: this is an experience report from January to October 2019, carried out at the Laboratory of Educational Technologies of a state public university in northern Brazil. Results: an integrative literature review was carried out, obtaining eight articles and the corpus was processed in the IRAMUTEQ software, which generated five classes. Based on the results of the integrative review, the application production was developed, meeting the functionality, usability, reliability, efficiency, maintainability and portability requirements. Conclusion: the experience of producing an educational technology based on scientific evidence and on a systems model, in the format of a mobile application, will provide the target audience of parents with access to updated and scientific content about safe care for newborns.

Objetivo: relatar la trayectoria de producción de una tecnología educativa sobre el cuidado del recién nacido para padres primerizos en forma de aplicación para dispositivo móvil. Método: relato de experiencia de enero a octubre de 2019, realizado en el Laboratorio de Tecnologías Educativas de una universidad pública estatal de la región Norte de Brasil. Resultados: se realizó una revisión integrativa de la literatura, obteniendo ocho artículos y el corpus fue procesado en el software IRAMUTEQ, que generó cinco clases. Con base en los resultados de la revisión integradora, se desarrolló la producción de la aplicación, cumpliendo con los requisitos de funcionalidad, usabilidad, confiabilidad, eficiencia, mantenibilidad y portabilidad. Conclusión: la experiencia de producir una tecnología educativa basada en evidencia científica y modelo de sistemas, en formato de aplicación móvil, permitirá que el público objetivo de los padres tenga acceso a contenidos actualizados y científicos sobre el cuidado seguro del recién nacido.