Detection of tick-borne encephalitis virus in ixodid ticks collected in natural foci by time-resolved fluoroimmunoassay.

Time-resolved fluoroimmunoassay (TR-FIA) was used for the first time for evaluation of infestation of ixodid ticks with tick-borne encephalitis virus. Comparison of TR-FIA results with those obtained in enzyme immunoassay and by virus isolation confirmed the high efficacy of the method in question. Positive results of TR-FIA coincided with the data of virus isolation in 83.6% cases, the level of false-negative results did not exceed 1.2%, the overall time consumption amounted to about 1.2 hr.

[Demonstration of the tick-borne encephalitis virus by an immunoenzyme method during a study of its persistence and the chronicity of the process in experimental animals]. / Indikatsiia virusa kleshchevogo éntsefalita immunofermentnym metodom v khode izucheniia persistentsii i khronizatsii protsessa u éksperimental'nykh zhivotnykh.

The studies carried out by the authors indicated the advantages of the enzyme-immunoassay (EIA) for the detection of the persisting tick-borne encephalitis (TBE) virus and in verification of the chronic course of TBE as compared with HA test, HI test and biologic neutralization test. This method detected TBE virus in the brain of most animals developing the disease within 30-300 days postinoculation. In mice developing no disease the persisting virus was detected both directly in the organs and after preliminary amplification in a sensitive biological model and by the tissue explant method. The EIA was particularly effective in the study of persistence at later intervals (100-300 days), when the hemagglutinating and infective activities of the virus declined. The method is more informative, specific and less labor-consuming.

[Results of using the ELISA method in studying tick-borne encephalitis and rabies]. / Rezul'taty ispol'zovaniia metoda ELISA pri izuchenii kleshchevogo éntsefalita i beshenstva.

The results of using the indirect variant of ELISA for the study of serum samples from humans and white mice for the presence of antibodies to tick-borne encephalitis virus and rabies virus are presented. The high sensitivity and specificity of this method have been confirmed.