EOSINOPHILIC ANGIOCENTRIC FIBROSIS AS A CAUSE OF NASAL OBSTRUCTION. A CASE REPORT.

Eosinophilic angiocentric fibrosis (EAF) is a rare benign lesion of unknown aetiology, found predominantly in the upper respiratory tract. The fibrosis must be confirmed by considering other lesions in the differential diagnosis. The typical histological characteristics are the basis for establishing the diagnosis. We present a patient with long-term nasal obstruction caused by EAF. The patient had repeated resections of the lesion for its recurrences. As follows from the literature, radical resection is crucial for the treatment of EAF. This procedure may be difficult at times given the extent and localization of the disease..

TRANSECTION OF INFERIOR ALVEOLAR NERVE IN THE RAT - NEUROANATOMICAL STUDY AND EXPERIMENTAL MODEL.

OBJECTIVE: Experimental lesions in the inferior alveolar nerve (IAN) are used for the study of peripheral and central alterations. The objective of our study was to contribute to a more precise description of the approach to the IAN and creating a lesion. MATERIAL AND METHODS: Twenty-six males of Wistar laboratory rats were used for the study. The animals were divided into three groups: control group (6 rats), experimental group (12 rats - a part of the bone above the mandibular canal was removed under general anaesthesia using extraoral approach, after exposing a part of the IAN, the nerve was excised in a length of 3 mm), and a sham group (8 rats - the nerve was only dissected but not transected). Persisting denervation was verified using surgical revision and histological and immunohistochemical analysis after the observation period (4 weeks). RESULTS: No evidence of re-innervation after 4 weeks. We found no statistically significant differences in mean weight gains between individual groups during the observation period. CONCLUSION: The described technique used in the study is one of the possible ways to create a nerve lesion at the site of the main trunk of the nerve. At the same time, the study provides a more precise description of the anatomical situation and approach to the IAN in the mandibular canal.

Intraosseous haemangioma od the zygoma - case report and literature review.

Intraosseous haemangioma is a benign lesion that accounts for less than 1% of all bone tumours. It is found in the zygoma only rarely. This type of tumour is more common in women than in men. The paper presents the case of a female patient with intraosseous cavernous haemangioma in the zygoma. The tumour caused a deformation of the zygoma on the left. The lesion was visible in computed tomography (CT) scans, and also in magnetic resonance imaging (MRI) scans obtained previously due to problems unrelated to the lesion. We performed complete removal of the tumour including edges of healthy bone. The defect was bridge using a titanium miniplate. A good cosmetic effect was seen after 5 months from the surgery. Follow up CT and MRI evaluation showed no recurrence or residue of the tumour. The paper also includes a review of available literature.

Multielemental Chemical Analysis of Elements in Mandibular Bone and Teeth in the Rat.

The purpose of the study was to test the hypothesis of different distribution spaces of elements in the rat mandibular bone and teeth. We used six adult males of Wistar laboratory rats for the study. After killing the animals, we extracted the molars and removed incisor crowns. The mandibular bone was divided into four parts (mesial-central-distal-ridge). Inductively coupled plasma mass spectrometry was used to determine the presence of 41 elements in the bone and tooth. Evidence of 14 elements was found in all samples (incisors-molarsbone). Generally, significant differences between the left and right side were found for K and Rb in the bone locations. As regards statistically significant differences in incisors-molars-bone locations, the elements for which these differences were found for all comparisons are listed as incisors versus individual molars, incisors versus bone locations, and individual molars versus bone locations: a) incisors-molars: Ba, Mn, Mo, Sr, Zn, K, Mg and Rb; b) incisors-bone: Fe, K, Mg, Mn, Na, Zn and Ba; c) molars-bone: Mn, Mo, Na and Mg. Statistically significant differences were also found between molars for Fe, Mg, Mn, and Sr and between bone locations for Ba, Ca, Mn, Sr, K, Rb, Zn, Mo, Mg, and Na. The elements Cu, Ni and Co were without pronounced differences. Twenty-seven elements were below the detection limit. Our results indicate different distributions of some elements in the rat mandibular incisors-molars-bone. We assume that the knowledge of chemical element contents in the laboratory rat bone and teeth will prove useful in experimental research of both these hard tissues.

Multielemental Chemical Analysis of Elements in Mandibular Bone and Teeth in the Rat.

The original article was published in Folia Biologica (Praha) Volume 64, No. 3 (2018), 84-96.

UPPER EYELID INJURY WITH PARTIAL LOSS. CASE REPORT.

In case of an upper eyelid injury with loss of tissue, it is very important to perform reconstruction to restore protection of the cornea and bulbus. Depending on the size and location of the defect the most suitable reconstructive technique is chosen. This case report presents a patient with a partial loss of the upper eyelid. The defect was reconstructed with a transposition skin flap to cover the ear cartilage graft. The missing conjunctiva was partially reconstructed by its mobilization from the fornix, and a part of the defect was left to heal by spontaneous epithelisation. This procedure provides an alternative solution for defects in the lateral part of the upper eyelid.

USE OF OSTEOTOMY IN POST-TRAUMATIC DEFORMITY OF FRONTAL SINUS ANTERIOR WALL. CASE REPORT.

Post-traumatic deformity of frontal sinus anterior table can be treated in various ways. Usage of a certain type of an implant is a common method. We performed an osteotomy and subsequent reduction of the fragments to reconstruct their anatomical position. Titanium miniplates and screws were used for fixation. This case report suggests a possible use of this method in patients with more extensive frontal sinus and distinct post-traumatic deformity. No similar case manages as described in our case report was found in the literature.

Reconstruction of a nasal tip defect using a V-Y island dorsal nasal flap with unilateral vascular supply. A case report.

Closure of a nasal tip defect has specific characteristics with regards to the shape, colour, thickness and texture of skin. During the reconstruction it is necessary to observe aesthetic and functional characteristics to achieve an optimal result. There are several methods for closure of a defect in this area. One of the possible techniques is usage of a local flap. In the selection of an optimal procedure it is suitable to consider the aesthetic subunits in the nose. Usage of the V-Y flap is beneficial compared to a rotation flap or a transposition flap, which are associated with significant scaring and deformities, such as a dog-ear. Such conditions may require further corrections later. Erçöçen et al. described a V-Y island dorsal nasal flap, which they used to close a nasal tip defect. The blood supply of the flap was based on the terminal branches of angular artery on both sides (1). In our case report we used the aforementioned flap to close a nasal defect. The flap was based on the terminal branches of the angular artery only on one side.

Systematic study of Mn-doping trends in optical properties of (Ga,Mn)As.

We report on a systematic study of optical properties of (Ga,Mn)As epilayers spanning the wide range of accessible Mn(Ga) dopings. The material synthesis was optimized for each nominal Mn doping in order to obtain films which are as close as possible to uniform uncompensated (Ga,Mn)As mixed crystals. We observe a broad maximum in the mid-infrared absorption spectra whose position exhibits a prevailing blueshift for increasing Mn doping. In the visible range, a peak in the magnetic circular dichroism also shifts with increasing Mn doping. The results are consistent with the description of ferromagnetic (Ga,Mn)As based on the microscopic valence band theory. They also imply that opposite trends seen previously in the optical data on a limited number of samples are not generic and cannot serve as an experimental basis for postulating the impurity band model of ferromagnetic (Ga,Mn)As.

Co(II)-Based single-ion magnets with 1,1'-ferrocenediyl-bis(diphenylphosphine) metalloligands.

Herein, we report on investigations of magnetic and spectroscopic properties of three heterobimetallic Fe(ii)-Co(ii) coordination compounds based on the tetracoordinate {CoP2X2} core encapsulated by dppf metalloligand, where X = Cl (1), Br (2), I (3), dppf = 1,1'-ferrocenediyl -bis(diphenylphosphine). The analysis of static magnetic data has revealed the presence of axial magnetic anisotropy in compounds (1) and (2) and this was further confirmed by high-frequency electron spin resonance (HF-ESR) spectroscopy. Dynamic magnetic data confirmed that (1) and (2) behave as field-induced Single-Ion Magnets (SIMs). Together with bulk studies, we have also tested the possibility of depositing (2) as thick films on Au(111), glass, and polymeric acetate by drop-casting as well as thermal sublimation, a key aspect for the development of future devices embedding these magnetic objects.

Two polymorphic Co(ii) field-induced single-ion magnets with enormous angular distortion from the ideal octahedron.

A mononuclear complex [Co(neo)(PhCOO)2,], neo = neocuproine, PhCOO- = the benzoate anion, was prepared in two polymorph forms crystallizing in the C2/c, (1) and P21/c, (2) space groups. The polymorphs differ in the Co-O bond lengths and the level of trigonal distortion of their coordination polyhedra. The static and dynamic magnetic properties of these compounds were thoroughly studied by experimental (magnetometry) and theoretical (ab initio calculations) methods. The analysis of magnetic data was performed using the spin Hamiltonian formalism or the L-S model considering also the orbital angular momentum. It was revealed that both polymorphs possess a very large magnetic anisotropy with a pronounced rhombic character leading to the separation of the Kramers doublets larger than 120 cm-1. The measurements of alternating current susceptibility revealed that both polymorphs behave as field induced single molecule magnets with a small barrier of spin reversal (U = 22.1 K (for 1) and 17.1 K (for 2)) which indicates that relaxation processes other than the thermally activated Orbach process take place.

Ferromagnetic coupling mediated by Coπ non-covalent contacts in a pentacoordinate Co(ii) compound showing field-induced slow relaxation of magnetization.

[Co(II)(dpt)(NCS)2], where dpt = bis(3-aminopropyl)amine, was identified as a pentacoordinate Co(II) compound showing field-induced slow relaxation of magnetization. Furthermore, intermolecular ferromagnetic coupling mediated by Coπ non-covalent contacts, where π orbitals originate from the thiocyanato ligand, is reported for the first time.

Determination of chlorpromazine and thioridazine by differential pulse voltammetry in acetonitrile medium.

This work describes optimal conditions for the determination of chlorpromazine and thioridazine by DPV, with a detection limit of 4.0 x 10(-7)M (0.16 microg/ml). The well-defined peaks indicate that direct measurement in a medium of 0.03 M perchloric acid in acetonitrile is possible and the use of DPV is advantageous when small amounts are to be determined. The sample procedure for pharmaceutical tablets is simple and fast. The method is useful for control of the quality and purity of phenothiazine drugs. The possibility of using numerical analysis to resolve composite signals of the compounds studied has also been demonstrated.

Antifungal properties of substituted 1-phenyl-5-mercaptotetrazoles and their oxidation product, 5-bis-(1-phenyltetrazolyl)disulfide.

The antifungal effect of substituted 1-phenyl-5-mercaptotetrazoles was tested with Candida tropicalis, C. pseudotropicalis, C. mogii, Trichosporon cutaneum, Cryptococcus albidus and S. cerevisiae. Candida strains exhibited the lowest sensitivity to the compounds; the most sensitive was S. cerevisiae. The MIC values ranged from 40 to > 1000 mg/mL. The antifungal effect of halogenated compounds decreased in the series of bromo > chloro > fluoro derivatives. The electrochemical oxidation of substituted 1-phenyl-5-mercaptotetrazole derivatives in an acetonitrile medium was studied as a model for the enzymic oxidation of the substance, including study of the effect of water, perchloric and trifluoromethanesulfuric acids on E1/2 and I1. 5-Bis-(1-phenyltetrazolyl)disulfide, the compound with no antifungal effect, has been identified as the main oxidation product of 1-phenyl-5-mercaptotetrazole.

Antifungal effects of new heterocyclic compounds, 6H-pyrimido[2,1-a]isoindole derivatives.

Minimal inhibitory concentrations for 10 new condensed isoindole derivatives were established by the agar diffusion method. Benzo[b]-6H-pyrimido[2,1-a]isoindole-4-one exhibited a broad antifungal effect; the observed MIC (mg/L) were 7 (K. lactis), 8 (C. pseudotropicalis), 17 (W. fluorescens), 22 (E. magnusii), 31 (P. membranaefaciens), 32 (S. alluvius) and 32 (S. cerevisiae). The compound also inhibited the growth of B. subtilis (MIC 9 mg/L). No effect on the growth of E. coli was found.

Effect of structure on antibiotic action of new 9-(ethylthio)acridines.

Six new 9-(ethylthio)acridine derivatives were examined for antibacterial and antifungal activities with 10 bacterial and 8 yeast strains. The only active compounds were 2- and 3-amino derivatives. The observed MICs (mg/L) for 2-amino-9-(ethylthio)acridine (possessing the highest biological activity) were 12 (P. mirabilis), 30 (B. subtillis), 60 (C. freundii), 90 (E. coli), 128 (E. vulneris) and 500 (S. marcescens and S. aureus). Both amino derivatives have also lowest half-wave potential (E1/2) and field Swain-Lupton constants (describing oxidoreduction behavior) what supports the importance of acridine ion formation in the mechanism of antimicrobial action.

[A dexterity test in school-age children]. / Test obratnosti u detí skolního veku.

In a group of 50 children of school age a dexterity test was performed. The group comprised children who, when born, were mature, immature or hypotrophic. The authors describe the method of the test, its physiological background and value. From the results of the test ensues that there is no difference between boys and girls, no difference in practice and gnosis, no difference between the right and left hand, the result of the test does not depend on age. Maturity, prematurity or hypotrophy caused significant differences in the results of the test. As according to statistical criteria the group is sufficiently numerous, the submitted results of the test can be used as standards in school children who were born mature, premature or hypotrophic. The submitted battery of tests is a suitable method for testing of dexterity. In the conclusion the authors give output data for paediatric and teaching practice.

Analysis of organic colouring and binding components in colour layer of art works.

Two methods of analysis of organic components of colour layers of art works have been tested: IR microspectroscopy of indigo, Cu-phthalocyanine, and Prussian blue, and MALDI-TOF-MS of proteinaceous binders and a protein-containing red dye. The IR spectra distortion common for smooth outer surfaces and polished cross sections of colour layer of art works is suppressed by reflectance measurement of microtome slices. The detection limit of the three blue pigments examined is approximately 0.3 wt% in reference colour layers in linseed oil binder with calcite as extender and lead white as a drying agent. The sensitivity has been sufficient to identify Prussian blue in repaints on a Gothic painting. MALDI-TOF-MS has been used to identify proteinaceous binders in two historical paintings, namely isinglass (fish glue) and rabbit glue. MALDI-TOF-MS has also been proposed for identification of an insect red dye, cochineal carmine, according to its specific protein component. The enzymatic cleavage with trypsin before MALDI-TOF-MS seems to be a very gentle and specific way of dissolution of the colour layers highly polymerised due to very long aging of old, e.g. medieval, samples.

The clinical contribution of a uniform diagnostic and therapeutic protocol in Hodgkin's disease.

In the period between 5 July, 1968 and 31 December, 1986, a total of 138 patients with Hodgkin's disease were treated at the Institute of Biophysics and Nuclear Medicine of the -- School of General Medicine of Charles University in Prague. In the initial period between 5 July, 1968 and 31 December, 1977 a 10-year survival of 28% and a disease-free survival of 64% were achieved in 29 selected patients. In the next period, the patients were diagnosed and treated according to the uniform protocol used by all centres in the Czech Socialist Republic concentrated in the Lymphoma Cooperative Group. In the whole follow-up period, 5-year and 10-year survival rates of 78% and 75%, respectively, were achieved in 112 surviving persons. The corresponding survival rates in complete remission are 65.5% and 62.5%, respectively. When compared to survival of our patients treated up to 1974, the 5-year survival increased from 40% to 70% in 1986. Improvement of therapeutic outcome is due to more effective treatment. Increased diagnostic accuracy and, thus, also improved disease classification, enables the choice of optimal treatment. The treatment efficacy is documented by restoration of the working capacity in more than a half (51.17%) of patients in our group.