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J Immunol ; 180(6): 4330-7, 2008 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-18322247

RESUMO

Soluble HLA-G (sHLA-G) secretion by human preimplantation embryos in culture has been associated with successful embryo development, and therefore has potential to serve as a noninvasive marker of embryo viability. We have examined the spatial and temporal expression of HLA-G in embryos of varying developmental competence and the role of maternal factors in human embryonic HLA-G expression. Embryos that reached blastocyst stage on day 5 showed a higher frequency of sHLA-G secretion than those at morula or arrested stages (p < 0.05). There was no significant difference in sHLA-G secretion between normal embryos and those diagnosed as chromosomally abnormal by preimplantation genetic diagnosis. HLA-G detected in maternal plasma and follicular fluid did not appear to correlate with HLA-G expressed in the embryo or embryo supernatants. Confocal microscopy analysis indicated that HLA-G protein expression in embryos was not homogeneous; mostly, it was confined to blastocysts localized on trophectoderm and trophectoderm projections. Single-particle fluorescent imaging analysis of HLA-G on the cell surface of JEG-3 cells showed that HLA-G particles were mostly monomeric, but dimeric and higher order oligomers were also observed. These results suggest that HLA-G play an important role in preimplantation embryo development. However, the observed expression of HLA-G in arrested and chromosomally abnormal embryos indicates that HLA-G testing should be used with caution and in conjunction with conventional methods of embryo screening and selection.


Assuntos
Blastocisto/química , Blastocisto/imunologia , Líquido Folicular/química , Líquido Folicular/imunologia , Antígenos HLA/análise , Antígenos HLA/genética , Antígenos de Histocompatibilidade Classe I/análise , Antígenos de Histocompatibilidade Classe I/genética , Troca Materno-Fetal/imunologia , Diagnóstico Pré-Implantação , Blastocisto/metabolismo , Linhagem Celular Tumoral , Sistema Livre de Células/química , Sistema Livre de Células/imunologia , Sistema Livre de Células/metabolismo , Técnicas de Cultura Embrionária , Feminino , Líquido Folicular/metabolismo , Antígenos HLA/biossíntese , Antígenos HLA/sangue , Antígenos HLA-G , Antígenos de Histocompatibilidade Classe I/biossíntese , Antígenos de Histocompatibilidade Classe I/sangue , Humanos , Microscopia Confocal , Microscopia de Fluorescência , Óvulo/química , Óvulo/imunologia , Óvulo/metabolismo , Gravidez , Diagnóstico Pré-Implantação/instrumentação , Diagnóstico Pré-Implantação/métodos
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