RESUMO
The DNA content in epitheliocytes of the mucous membrane of the oral cavity and expression of carcinoembryonic antigen and trophoblast-specific beta-1 glycoprotein on peripheral blood lymphocytes, peripheral blood T-lymphocytes subsets into three groups of patients: 1--diffusion mastopathy; 2--diffusion mastopathy with fibromyoma uteri and 3--fibroadenoma were studied. It was indicated that DNA content was higher in all groups in comparison with healthy women and it was shown that in contrast to healthy women, 45% of patients studied had abnormal rate of T-cells subsets, about 30% of them had abnormal expression of CEA and TSG on peripheral blood lymphocytes. The data obtained show the significant disbalance in the mechanisms of defence and compensation taking place in the organisms of patients with nonmalignant mammary diseases.
Assuntos
Antígenos de Neoplasias/sangue , DNA de Neoplasias/análise , DNA/análise , Doença da Mama Fibrocística/genética , Doença da Mama Fibrocística/imunologia , Mucosa Bucal/química , Linfócitos T/imunologia , Adulto , Neoplasias da Mama/genética , Neoplasias da Mama/imunologia , Células Epiteliais/química , Feminino , Fibroadenoma/genética , Fibroadenoma/imunologia , Humanos , Leiomioma/genética , Leiomioma/imunologia , Contagem de Linfócitos , Pessoa de Meia-Idade , Neoplasias Uterinas/genética , Neoplasias Uterinas/imunologiaRESUMO
The cervical dysplasia and cancer of the uterine cervix of 82 patients have been studied by the cyto-fluorimetric technique. There were 46 patients with dysplasia of mild, moderate and severe degree and 36 patients with different histopathologic type cancer of the uterine cervix T1-2N0M0. DNA content was determined in cytological smears and scrapings of cervix uterus epithelium using luminescent microscope equipped with photometric device FMEI-1. The mean DNA content under dysplasia of cervix uterus reliably differed from that in control (3.504 +/- 0.054 and 4.715 +/- 0.051, respectively). The level of DNA content was heterogenic and elevated in cases of intraepithelial cancer and carcinoma in situ (8.369 +/- 0.84 and 10.098 +/- 0.28, respectively). This value was considerably higher than tumor progression (9,423 +/- 0.051).