RESUMO
A multi-laboratory broth microdilution method trial was performed to standardize the specialized test conditions required for the fish pathogens Flavobacterium columnare and F. psychrophilum. Nine laboratories tested the quality control (QC) strains Escherichia coli ATCC 25922 and Aeromonas salmonicida subsp. salmonicida ATCC 33658 against 10 antimicrobials (ampicillin, enrofloxacin, erythromycin, florfenicol, flumequine, gentamicin, ormetoprim/sulfadimethoxine, oxolinic acid, oxytetracycline, and trimethoprim/sulfamethoxazole) in diluted (4 g l-1) cation-adjusted Mueller-Hinton broth incubated at 28 and 18°C for 44-48 and 92-96 h, respectively. QC ranges were set for 9 of the 10 antimicrobials. Most of the minimal inhibitory concentration (MIC) distributions (16 of 18, 9 drugs at both temperatures) for A. salmonicida ATCC 33658 were centered on a single median MIC ± 1 two-fold drug dilution resulting in a QC range that spanned 3 dilutions. More of the E. coli ATCC 25922 MIC distributions (7 of 16) were centered between 2 MIC dilutions requiring a QC range that spanned 4 dilutions. A QC range could not be determined for E. coli ATCC 25922 against 2 antimicrobials at the low temperature. These data and their associated QC ranges have been approved by the Clinical and Laboratory Standards Institute (CLSI), and will be included in the next edition of the CLSI M49-A Guideline. This method represents the first standardized reference method for testing fish pathogenic Flavobacterium spp.
Assuntos
Antibacterianos/farmacologia , Farmacorresistência Bacteriana , Flavobacterium/efeitos dos fármacos , Testes de Sensibilidade Microbiana/métodos , Animais , Reprodutibilidade dos Testes , Sensibilidade e EspecificidadeRESUMO
Escherichia albertii has been associated with diarrhea in humans but not with disease or infection in animals. However, in December 2004, E. albertii was found, by biochemical and genetic methods, to be the probable cause of death for redpoll finches (Carduelis flammea) in Alaska. Subsequent investigation found this organism in dead and subclinically infected birds of other species from North America and Australia. Isolates from dead finches in Scotland, previously identified as Escherichia coli O86:K61, also were shown to be E. albertii. Similar to the isolates from humans, E. albertii isolates from birds possessed intimin (eae) and cytolethal distending toxin (cdtB) genes but lacked Shiga toxin (stx) genes. Genetic analysis of eae and cdtB sequences, multilocus sequence typing, and pulsed-field gel electrophoresis patterns showed that the E. albertii strains from birds are heterogeneous but similar to isolates that cause disease in humans.
Assuntos
Animais Domésticos/microbiologia , Animais Selvagens/microbiologia , Aves/microbiologia , Infecções por Enterobacteriaceae/veterinária , Escherichia , Animais , Galinhas/microbiologia , Patos/microbiologia , Eletroforese em Gel de Campo Pulsado , Endotoxinas/genética , Infecções por Enterobacteriaceae/microbiologia , Escherichia/genética , Tentilhões/microbiologia , Gansos/microbiologia , Genes Bacterianos/genética , Dados de Sequência Molecular , Passeriformes/microbiologia , Reação em Cadeia da Polimerase , Análise de Sequência de DNA , Fatores de Virulência/genéticaRESUMO
The Oriental white-backed vulture (OWBV; Gyps bengalensis) was once one of the most common raptors in the Indian subcontinent. A population decline of >95%, starting in the 1990s, was first noted at Keoladeo National Park, India. Since then, catastrophic declines, also involving Gyps indicus and Gyps tenuirostris, have continued to be reported across the subcontinent. Consequently these vultures are now listed as critically endangered by BirdLife International. In 2000, the Peregrine Fund initiated its Asian Vulture Crisis Project with the Ornithological Society of Pakistan, establishing study sites at 16 OWBV colonies in the Kasur, Khanewal and Muzaffargarh-Layyah Districts of Pakistan to measure mortality at over 2,400 active nest sites. Between 2000 and 2003, high annual adult and subadult mortality (5-86%) and resulting population declines (34-95%) (ref. 5 and M.G., manuscript in preparation) were associated with renal failure and visceral gout. Here, we provide results that directly correlate residues of the anti-inflammatory drug diclofenac with renal failure. Diclofenac residues and renal disease were reproduced experimentally in OWBVs by direct oral exposure and through feeding vultures diclofenac-treated livestock. We propose that residues of veterinary diclofenac are responsible for the OWBV decline.
Assuntos
Doenças das Aves/induzido quimicamente , Diclofenaco/intoxicação , Aves Predatórias/fisiologia , Animais , Anti-Inflamatórios não Esteroides/administração & dosagem , Anti-Inflamatórios não Esteroides/intoxicação , Autopsia/veterinária , Doenças das Aves/diagnóstico , Doenças das Aves/epidemiologia , Doenças das Aves/patologia , Causas de Morte , Conservação dos Recursos Naturais , Diclofenaco/administração & dosagem , Dieta , Resíduos de Drogas/intoxicação , Cadeia Alimentar , Gota/induzido quimicamente , Gota/complicações , Gota/epidemiologia , Gota/veterinária , Fígado/química , Fígado/patologia , Paquistão/epidemiologia , Dinâmica Populacional , Insuficiência Renal/induzido quimicamente , Insuficiência Renal/epidemiologia , Insuficiência Renal/patologia , Insuficiência Renal/veterinária , Ácido Úrico/análise , Medicina VeterináriaRESUMO
Equine infectious anemia virus (EIAV), uniquely among lentiviruses, does not encode a vif gene product. Other lentiviruses, including human immunodeficiency virus type 1 (HIV-1), use Vif to neutralize members of the APOBEC3 (A3) family of intrinsic immunity factors that would otherwise inhibit viral infectivity. This suggests either that equine cells infected by EIAV in vivo do not express active A3 proteins or that EIAV has developed a novel mechanism to avoid inhibition by equine A3 (eA3). Here, we demonstrate that horses encode six distinct A3 proteins, four of which contain a single copy of the cytidine deaminase (CDA) consensus active site and two of which contain two CDA motifs. This represents a level of complexity previously seen only in primates. Phylogenetic analysis of equine single-CDA A3 proteins revealed two proteins related to human A3A (hA3A), one related to hA3C, and one related to hA3H. Both equine double-CDA proteins are similar to hA3F and were named eA3F1 and eA3F2. Analysis of eA3F1 and eA3F2 expression in vivo shows that the mRNAs encoding these proteins are widely expressed, including in cells that are natural EIAV targets. Both eA3F1 and eA3F2 inhibit retrotransposon mobility, while eA3F1 is a potent inhibitor of a Vif-deficient HIV-1 mutant and induces extensive editing of HIV-1 reverse transcripts. However, both eA3F1 and eA3F2 are weak inhibitors of EIAV. Surprisingly, eA3F1 and eA3F2 were packaged into EIAV and HIV-1 virions as effectively as hA3G, although only the latter inhibited EIAV infectivity. Moreover, all three proteins bound both the HIV-1 and EIAV nucleocapsid protein specifically in vitro. It therefore appears that EIAV has evolved a novel mechanism to specifically neutralize the biological activities of the cognate eA3F1 and eA3F2 proteins at a step subsequent to virion incorporation.
Assuntos
Citidina Desaminase/fisiologia , HIV-1/imunologia , Cavalos/imunologia , Vírus da Anemia Infecciosa Equina/imunologia , Montagem de Vírus , Desaminase APOBEC-1 , Sequência de Aminoácidos , Animais , Mapeamento Cromossômico , Citidina Desaminase/química , Citidina Desaminase/genética , HIV-1/genética , Células HeLa , Humanos , Imunidade Inata , Dados de Sequência Molecular , Estrutura Terciária de Proteína , Retroelementos , Replicação ViralRESUMO
The aim of this study was to identify tissues where ovine herpesvirus 2 (OvHV-2) replication occurs in vivo. A reverse-transcriptase PCR targeting the OvHV-2 major capsid protein gene (ORF 25) was developed and the presence of transcripts used as an indicator of virus replication in naturally infected sheep, and cattle and bison with sheep-associated malignant catarrhal fever (SA-MCF). ORF 25 transcripts were detected in 18 of 60 (30%) turbinate, trachea, and lung samples from five sheep experiencing a shedding episode; 12 of the 18 positive samples were turbinates. ORF 25 transcripts were not detected in any other tissue from the shedding sheep (n=55). In contrast, 86 of 102 (84%) samples from clinically affected bovine and bison tissues, including brain, kidney, intestine, and bladder, had ORF 25 transcripts. The data strongly suggest that OvHV-2 replication is localized to the respiratory tract of shedding sheep, predominantly in the turbinate, while it occurs in virtually all tissues of cattle and bison with SA-MCF. These findings represent an important initial step in understanding viral pathogenesis, and in potentially establishing a system for OvHV-2 propagation in vitro.
Assuntos
Proteínas do Capsídeo/genética , Infecções por Herpesviridae/veterinária , Rhadinovirus/genética , Doenças dos Ovinos/virologia , Replicação Viral , Eliminação de Partículas Virais , Estruturas Animais/virologia , Animais , Bison , Proteínas do Capsídeo/metabolismo , Bovinos , Doenças dos Bovinos/fisiopatologia , Doenças dos Bovinos/virologia , Infecções por Herpesviridae/transmissão , Infecções por Herpesviridae/virologia , Microscopia Eletrônica de Transmissão , Fases de Leitura Aberta , RNA Viral/genética , RNA Viral/isolamento & purificação , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Rhadinovirus/fisiologia , Rhadinovirus/ultraestrutura , Ovinos , Doenças dos Ovinos/transmissão , Conchas Nasais/virologiaRESUMO
A herpesviral disease of Rock Pigeons (Columba livia), called "inclusion body disease" or "inclusion body hepatitis," was first described in the 1940s. The disease involves hepatic and splenic necrosis with associated intranuclear inclusion bodies and occurs primarily in young squabs. A similar herpesviral disease occurs in falcons and owls. Serologic and restriction endonuclease digestion studies indicate that herpesviruses from pigeons, falcons, and owls are very closely related and that most reported cases of disease in falcons and owls involve prior documented or possible ingestion of pigeons. These findings led to the hypothesis that an endemic herpesvirus of pigeons may be causing disease in falcons and owls. In order to test this hypothesis, we sequenced a fragment of the herpesviral DNA polymerase gene from naturally infected owls, falcons, and pigeons with inclusion body disease collected between 1991 and 2006. Sequences from all three sources were almost identical, and we therefore propose that the usual agent of inclusion body hepatitis in owls and falcons is columbid herpesvirus 1.
Assuntos
Doenças das Aves/virologia , Falconiformes/virologia , Infecções por Herpesviridae/veterinária , Herpesviridae/classificação , Corpos de Inclusão Viral , Estrigiformes/virologia , Animais , Sequência de Bases , Doenças das Aves/epidemiologia , Doenças das Aves/transmissão , Columbidae/virologia , DNA Viral/química , Herpesviridae/genética , Infecções por Herpesviridae/epidemiologia , Infecções por Herpesviridae/transmissão , Infecções por Herpesviridae/virologia , Mapeamento por Restrição/veterinária , Análise de Sequência de DNA/veterinária , Especificidade da EspécieRESUMO
Infection of clinically susceptible ruminants, including domesticated cattle and American bison, with ovine herpesvirus 2 (OvHV-2) can result in the fatal lymphoproliferative and vasculitis syndrome known as malignant catarrhal fever (MCF). A reliable experimental infection model is needed to study the pathogenesis of MCF and to develop effective vaccination strategies to control the disease. An experimental aerosol infection model using sheep, the natural carriers of OvHV-2, has been developed (Taus et al., 2005). Using the protocol and OvHV-2 inoculum established in the previous study, eight calves were nebulized with four different doses of OvHV-2 in nasal secretions from infected sheep. Two control calves were nebulized with nasal secretions from uninfected sheep. Infection status of all calves was monitored using competitive inhibition ELISA, PCR and clinical parameters. Six of eight nebulized calves became infected with OvHV-2. One calf receiving the highest dose of virus developed typical clinical, gross and histological changes of MCF. This study showed that nasal secretions collected from sheep experiencing OvHV-2 shedding episodes were infectious for cattle and capable of inducing MCF. The data also indicate that cattle are relatively resistant to disease following infection. The use of more susceptible species as experimental animal models, such as bison and selected cervid species should be examined.
Assuntos
Aerossóis/administração & dosagem , Herpesviridae/fisiologia , Febre Catarral Maligna/transmissão , Cavidade Nasal/virologia , Ovinos/virologia , Animais , Encéfalo/citologia , Encéfalo/patologia , Bovinos , Dexametasona , Masculino , Febre Catarral Maligna/patologia , Febre Catarral Maligna/virologia , Rúmen/citologia , Rúmen/patologia , Bexiga Urinária/citologia , Bexiga Urinária/patologia , Ativação Viral/fisiologiaRESUMO
Four female and 3 male Taita falcons (Falco fasciinucha) out of a breeding colony of 14 Taita falcons (7 pairs) died during the breeding season after showing lethargy and anorexia for 1 to 2 days. All animals were submitted for necropsy. Gross lesions in the female falcons were characterized by anemia secondary to marked hemorrhage into the ovary and oviduct, serofibrinous effusion into the cardioabdominal cavity and serosal petechiae. In addition, marked necrotizing splenitis and pulmonary hemorrhage were present. Histologically, the female falcons had mild necrotizing hepatitis with numerous intranuclear inclusion bodies and necrotizing splenitis with rare inclusion bodies. There were no gross lesions in the male falcons, and the histological lesions were characterized by urate deposition and rare intranuclear inclusion bodies in the renal tubular epithelial cells. Adenoviral particles were found by electron microscopy in the cloacal contents of the female Taita falcons but not in the male falcons. DNA in situ hybridization revealed widespread aviadenoviral nucleic acid within the nuclei of hepatocytes, renal tubular epithelial cells, and adrenal cells in the female falcons but no aviadenoviral nucleic acid in 1 male falcon and only a low quantity of adenoviral nucleic acid in the liver and kidney of another male Taita falcon. PCR amplified aviadenoviral DNA in the liver and intestine of all Taita falcons. The amplicons were sequenced, and the virus was identified as falcon adenovirus. The deaths of the female and male birds were attributed to the aviadenovirus infection.
Assuntos
Infecções por Adenoviridae/veterinária , Aviadenovirus/isolamento & purificação , Doenças das Aves/virologia , Surtos de Doenças/veterinária , Falconiformes , Infecções por Adenoviridae/epidemiologia , Infecções por Adenoviridae/patologia , Infecções por Adenoviridae/virologia , Animais , Aviadenovirus/genética , Doenças das Aves/epidemiologia , Doenças das Aves/patologia , DNA Viral/química , DNA Viral/genética , Feminino , Histocitoquímica/veterinária , Fígado/patologia , Fígado/ultraestrutura , Fígado/virologia , Masculino , Microscopia Eletrônica de Transmissão , Reação em Cadeia da Polimerase/veterinária , Baço/patologia , Baço/ultraestrutura , Baço/virologiaRESUMO
Alcelaphine herpesvirus-1 (AlHV-1) is a rhadinovirus that causes malignant catarrhal fever in certain ruminant species and is an important pathogen in Africa and other areas where carrier species and clinically susceptible ruminants intermingle. In this study, a real-time PCR for AlHV-1 DNA was developed and compared to an established nested PCR. The nested PCR amplifies a region of the AlHV-1 gene coding for a transactivator protein (ORF 50), while the real-time PCR assay targets the AlHV-1 gene coding for a tegument protein (ORF 3). The real-time PCR assay reproducibly detected 10 copies of target DNA. In a dilution series of the target DNA there was linearity of the assay across 8 orders of magnitude (10(1)-10(9) copies). The nested PCR was more sensitive (approximately with 1 log) than the real-time PCR. The assay specifically amplified samples containing only AlHV-1, but not other common herpesviruses of cattle. In conclusion, we have developed a rapid, relatively sensitive, and reliable real-time PCR assay specific for AlHV-1. Similar to the real-time PCR for Ovine herpesvirus-2, this assay should prove useful for differential diagnostics of clinical MCF and for research to better define the epidemiology of AlHV-1 in wildebeest as well as in animals with wildebeest-associated MCF.
Assuntos
Doenças dos Bovinos/diagnóstico , Gammaherpesvirinae/isolamento & purificação , Infecções por Herpesviridae/veterinária , Reação em Cadeia da Polimerase/métodos , Infecções Tumorais por Vírus/veterinária , Animais , Bovinos , Doenças dos Bovinos/virologia , Gammaherpesvirinae/genética , Infecções por Herpesviridae/diagnóstico , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Infecções Tumorais por Vírus/diagnóstico , Infecções Tumorais por Vírus/virologia , Proteínas Estruturais Virais/genéticaRESUMO
Caprine herpesvirus 2 (CpHV-2) is a recently recognized gammaherpesvirus that is endemic in domestic goats and has been observed to cause clinical malignant catarrhal fever (MCF) in certain species of deer. In this study, transmission of CpHV-2 in goats was examined. A total of 30 kids born to a CpHV-2 positive goat herd were selected and divided into two groups: group 1 (n=16) remained in the positive herd; group 2 (n=14) was separated from the herd at 1 week of age after obtaining colostrum. Peripheral blood samples from each kid were examined regularly by competitive ELISA for MCF viral antibody and by PCR for CpHV-2 DNA. Fifteen out of 16 goats (94%) that remained with the positive herd seroconverted and became PCR-positive for CpHV-2 by 10 months of age. In contrast, all kids (100%) that were separated from the positive herd at 1 week of age remained negative until termination of the experiment at 1 year of age. Additional transmission experiments revealed that all CpHV-2-free adult goats were susceptible to CpHV-2 or ovine herpesvirus 2 (OvHV-2) infection. The data indicate that the transmission pattern of CpHV-2 in goats is similar to the pattern of OvHV-2 in sheep and that CpHV-2-free goats can be established by early separation of kids from positive herds, which has significant implications for MCF control programs.
Assuntos
Criação de Animais Domésticos/métodos , Gammaherpesvirinae/fisiologia , Doenças das Cabras/transmissão , Infecções por Herpesviridae/veterinária , Doenças dos Ovinos/transmissão , Animais , Animais Recém-Nascidos , Anticorpos Antivirais/sangue , DNA Viral/análise , Doenças Endêmicas/prevenção & controle , Doenças Endêmicas/veterinária , Ensaio de Imunoadsorção Enzimática/veterinária , Gammaherpesvirinae/imunologia , Doenças das Cabras/prevenção & controle , Doenças das Cabras/virologia , Cabras , Infecções por Herpesviridae/prevenção & controle , Infecções por Herpesviridae/transmissão , Reação em Cadeia da Polimerase/veterinária , Distribuição Aleatória , Ovinos , Doenças dos Ovinos/virologiaRESUMO
A malignant catarrhal fever (MCF)-like disease was induced experimentally in 3 sheep after aerosol inoculation with ovine herpesvirus-2 (OvHV-2). Each of 3 OvHV-2-negative sheep was nebulized with 2 ml of nasal secretions containing approximately 3.07 X 10(9) OvHV-2 DNA copies from a sheep experiencing an intensive viral-shedding episode. Ovine herpesvirus-2 DNA became detectable by polymerase chain reaction in the peripheral blood leukocytes of all 3 sheep within 3 days, and all 3 seroconverted between 6 and 8 days postinfection (PI). The sheep developed clinical signs, with copious mucopurulent nasal discharge and fever around 14 days PI. One of the 3 clinically affected sheep was euthanized at 18 days PI. Major lesions at necropsy were multifocal linear erosions and ulcers in mucosa of the cheeks, tongue, pharynx, and proximal esophagus and mild disseminated pneumonia. Microscopically, there was extensive moderate superficial histiocytic-lymphocytic rhinitis with epithelial dissociation and degeneration. Moderate multifocal histiocytic bronchointerstitial pneumonia was associated with loss of terminal bronchiolar epithelium. Lymphocytic vasculitis was present only in the lung. The remaining 2 sheep recovered clinically, approximately 25 days PI. The study revealed that clinical signs and lesions resembling MCF can develop when uninfected sheep are exposed to a high dose of aerosolized OvHV-2.
Assuntos
Leucócitos/virologia , Febre Catarral Maligna/virologia , Mucosa Nasal/virologia , Rhadinovirus/crescimento & desenvolvimento , Doenças dos Ovinos/virologia , Animais , Bovinos , DNA Viral/análise , DNA Viral/sangue , Pulmão/patologia , Febre Catarral Maligna/diagnóstico , Febre Catarral Maligna/patologia , Mucosa Bucal/patologia , Mucosa Nasal/metabolismo , Mucosa Nasal/patologia , Reação em Cadeia da Polimerase , Rhadinovirus/genética , Ovinos , Doenças dos Ovinos/diagnóstico , Doenças dos Ovinos/patologiaRESUMO
Oriental white-backed vultures (Gyps bengalensis; OWBVs) died of renal failure when they ingested diclofenac, a nonsteroidal anti-inflammatory drug (NSAID), in tissues of domestic livestock. Acute necrosis of proximal convoluted tubules in these vultures was severe. Glomeruli, distal convoluted tubules, and collecting tubules were relatively spared in the vultures that had early lesions. In most vultures, however, lesions became extensive with large urate aggregates obscuring renal architecture. Inflammation was minimal. Extensive urate precipitation on the surface and within organ parenchyma (visceral gout) was consistently found in vultures with renal failure. Very little is known about the physiologic effect of NSAIDs in birds. Research in mammals has shown that diclofenac inhibits formation of prostaglandins. We propose that the mechanism by which diclofenac induces renal failure in the OWBV is through the inhibition of the modulating effect of prostaglandin on angiotensin II-mediated adrenergic stimulation. Renal portal valves open in response to adrenergic stimulation, redirecting portal blood to the caudal vena cava and bypassing the kidney. If diclofenac removes a modulating effect of prostaglandins on the renal portal valves, indiscriminant activation of these valves would redirect the primary nutrient blood supply away from the renal cortex. Resulting ischemic necrosis of the cortical proximal convoluted tubules would be consistent with our histologic findings in these OWBVs.
Assuntos
Anti-Inflamatórios não Esteroides/intoxicação , Doenças das Aves/induzido quimicamente , Diclofenaco/intoxicação , Resíduos de Drogas/intoxicação , Falconiformes , Insuficiência Renal/veterinária , Animais , Animais Selvagens , Doenças das Aves/metabolismo , Doenças das Aves/patologia , Causas de Morte , Diclofenaco/metabolismo , Cadeia Alimentar , Gota/induzido quimicamente , Gota/patologia , Gota/veterinária , Insuficiência Renal/induzido quimicamente , Insuficiência Renal/metabolismo , Insuficiência Renal/patologiaRESUMO
A 12-year-old American Saddlebred gelding was referred to a veterinary teaching hospital for evaluation of a chronic lameness problem in the right radiocarpal joint. The horse had been treated for osteoarthritis of the right radiocarpal joint with multiple injections of cortisone during the past 3 years. The horse was severely lame on the right forelimb at a trot. Radiography and computed tomography revealed a 3 x 2-cm lytic defect in the distal portion of the radius and periarticular bone proliferation around the right radiocarpal joint. Ultrasonography of the distal portion of the radius revealed a soft tissue mass in the palmarolateral aspect of the joint. Proliferative synovium with a large amount of fibrin was observed in the dorsal and palmar aspects of the joint via arthroscopic examination of the right radiocarpal joint. Histologic examination of synovial biopsy specimens revealed proliferative granulomatous synovitis with giant cells. Mycobacterium avium complex was cultured from the synovial fluid. Infection with M avium complex should be considered in horses with chronic recurring arthritis associated with granulomatous synovitis.
Assuntos
Artrite Infecciosa/veterinária , Doenças dos Cavalos/microbiologia , Complexo Mycobacterium avium , Infecção por Mycobacterium avium-intracellulare/veterinária , Sinovite/veterinária , Animais , Anti-Inflamatórios não Esteroides/uso terapêutico , Artrite Infecciosa/diagnóstico por imagem , Artrite Infecciosa/microbiologia , Artrite Infecciosa/terapia , Diagnóstico Diferencial , Granuloma/diagnóstico por imagem , Granuloma/microbiologia , Granuloma/veterinária , Doenças dos Cavalos/diagnóstico por imagem , Doenças dos Cavalos/tratamento farmacológico , Doenças dos Cavalos/cirurgia , Cavalos , Coxeadura Animal/etiologia , Coxeadura Animal/microbiologia , Masculino , Infecção por Mycobacterium avium-intracellulare/diagnóstico por imagem , Infecção por Mycobacterium avium-intracellulare/microbiologia , Infecção por Mycobacterium avium-intracellulare/terapia , Fenilbutazona/uso terapêutico , Sinovite/diagnóstico por imagem , Sinovite/microbiologia , Sinovite/terapia , Tomografia Computadorizada por Raios X/veterinária , Resultado do TratamentoRESUMO
Dogs may be infected by Mycobacterium (M.) tuberculosis, M. bovis, and M. avium complex, and the clinical signs associated with each of these infections may be indistinguishable. Rapid speciation of the infecting organism is desirable because of the public health concerns associated with M. bovis and M. tuberculosis infections. A mycobacterial infection was suspected in the dog of this report based on acid-fast staining of organisms in macrophages obtained from liver aspirates and buffy-coat preparations. Polymerase chain reaction (PCR) analysis of a buffy-coat preparation identified M. avium.
Assuntos
Doenças do Cão/diagnóstico , Mycobacterium avium/isolamento & purificação , Reação em Cadeia da Polimerase/veterinária , Tuberculose/veterinária , Animais , DNA Bacteriano/análise , Cães , Evolução Fatal , Feminino , Mycobacterium avium/classificação , Reação em Cadeia da Polimerase/métodos , Especificidade da Espécie , Tuberculose/diagnósticoRESUMO
BACKGROUND: Bronchopneumonia is a population limiting disease of bighorn sheep (Ovis canadensis). The cause of this disease has been a subject of debate. Leukotoxin expressing Mannheimia haemolytica and Bibersteinia trehalosi produce acute pneumonia after experimental challenge but are infrequently isolated from animals in natural outbreaks. Mycoplasma ovipneumoniae, epidemiologically implicated in naturally occurring outbreaks, has received little experimental evaluation as a primary agent of bighorn sheep pneumonia. METHODOLOGY/PRINCIPAL FINDINGS: In two experiments, bighorn sheep housed in multiple pens 7.6 to 12 m apart were exposed to M. ovipneumoniae by introduction of a single infected or challenged animal to a single pen. Respiratory disease was monitored by observation of clinical signs and confirmed by necropsy. Bacterial involvement in the pneumonic lungs was evaluated by conventional aerobic bacteriology and by culture-independent methods. In both experiments the challenge strain of M. ovipneumoniae was transmitted to all animals both within and between pens and all infected bighorn sheep developed bronchopneumonia. In six bighorn sheep in which the disease was allowed to run its course, three died with bronchopneumonia 34, 65, and 109 days after M. ovipneumoniae introduction. Diverse bacterial populations, predominantly including multiple obligate anaerobic species, were present in pneumonic lung tissues at necropsy. CONCLUSIONS/SIGNIFICANCE: Exposure to a single M. ovipneumoniae infected animal resulted in transmission of infection to all bighorn sheep both within the pen and in adjacent pens, and all infected sheep developed bronchopneumonia. The epidemiologic, pathologic and microbiologic findings in these experimental animals resembled those seen in naturally occurring pneumonia outbreaks in free ranging bighorn sheep.
Assuntos
Mycoplasma ovipneumoniae , Pneumonia/veterinária , Doenças dos Ovinos/epidemiologia , Animais , Pulmão/microbiologia , Pulmão/patologia , Mycoplasma ovipneumoniae/classificação , Mycoplasma ovipneumoniae/genética , RNA Ribossômico 16S/genética , Ovinos , Doenças dos Ovinos/diagnóstico , Doenças dos Ovinos/transmissão , Carneiro da MontanhaRESUMO
American bison (Bison bison) are particularly susceptible to developing fatal sheep-associated malignant catarrhal fever (SA-MCF) caused by ovine herpesvirus-2 (OvHV-2), a γ-herpesvirus in the Macavirus genus. This generally fatal disease is characterized by lymphoproliferation, vasculitis, and mucosal ulceration in American bison, domestic cattle (Bos taurus), and other clinically susceptible species which are considered non-adapted, dead-end hosts. The pathogenesis and cellular tropism of OvHV-2 infection have not been fully defined. An earlier study detected OvHV-2 open reading frame 25 (ORF25) transcripts encoding the viral major capsid protein in tissues of bison with SA-MCF, and levels of viral transcript expression positively correlated with lesion severity. To further define the cellular tropism and replication of OvHV-2 infection in vascular lesions of bison, immunofluorescence studies were performed to identify cell type(s) expressing ORF25 protein within tissues. Cytoplasmic and not nuclear ORF25 protein was demonstrated in predominantly perivascular fibroblasts in six bison with experimentally-induced SA-MCF, and there was no evidence of immunoreactivity in vascular endothelium, smooth muscle, or infiltrating leukocytes. The cytoplasmic distribution of viral major capsid protein suggests that viral replication in perivascular fibroblasts may be abortive in this dead-end host. These findings provide a novel foundation for defining the pathogenesis of vasculitis in non-adapted hosts with SA-MCF.
Assuntos
Bison/virologia , Fibroblastos/virologia , Gammaherpesvirinae/fisiologia , Febre Catarral Maligna/virologia , Doenças dos Ovinos/virologia , Vasculite/veterinária , Animais , Proteínas do Capsídeo/imunologia , Bovinos , Fibroblastos/patologia , Gammaherpesvirinae/isolamento & purificação , Febre Catarral Maligna/patologia , Fases de Leitura Aberta , Ovinos , Doenças dos Ovinos/patologia , Estados Unidos , Vasculite/patologia , Vasculite/virologia , Replicação ViralRESUMO
Bacteriologic cultures from oral, rectal, and lesion samples from free-ranging Steller sea lion (SSL, Eumetopias jubatus) pups and juveniles in Alaska (2001-2005) were examined to determine frequency of infection by a specific subset of common and pathogenic aerobic bacteria. Associations between isolated bacteria and age, sex, body condition, location, and sampling season were investigated. Salmonella spp. isolates were further evaluated to determine spatial clustering (n=48) and to identify serovars (n=13) and antimicrobial susceptibility patterns (n=11). We sampled 356 SSL pups (n=272) and juveniles (n=84), and identified 988 isolates of 13 bacterial genera of specific interest. Pasteurella spp. (43.8%), beta-hemolytic Streptococcus spp. (30.6%), and Mannheimia spp. (18.2%) were the most commonly isolated oral bacteria (n=499 isolates), whereas Escherichia coli (47.6%), beta-hemolytic E. coli (32.4%), Salmonella spp. (10.4%), and Campylobacter spp. (7.8%) were the most frequently isolated rectal bacteria (n=460 isolates). Salmonella was most commonly found in pups from western stocks and in samples collected during fall/winter seasons. A significant Salmonella cluster was detected at the Perry Island haulout. Five serovars were isolated: Enteritidis, Infantis, Newport, Reading, and Stanley. Pulsed-field gel electrophoresis provided evidence that Salmonella isolates were most likely being maintained within the SSL population in Alaska.
Assuntos
Boca/microbiologia , Reto/microbiologia , Salmonelose Animal/epidemiologia , Salmonella/isolamento & purificação , Leões-Marinhos/microbiologia , Fatores Etários , Alaska/epidemiologia , Animais , Animais Recém-Nascidos , Animais Selvagens , Feminino , Masculino , Salmonella/classificação , Estações do Ano , Fatores Sexuais , Especificidade da EspécieRESUMO
Malignant catarrhal fever (MCF), caused by ovine herpesvirus 2 (OvHV-2), is an important cause of mortality in ranched American bison and domestic cattle in North America. Previous studies showed that bison can be infected by intranasal nebulization with sheep nasal secretions containing OvHV-2 and provided preliminary information on viral doses required for infection and disease progression. The goals of this study were to establish optimal minimal infectious and minimal lethal doses of OvHV-2 by the intranasal route in bison, evaluate the influence of dose on incubation period and other clinical parameters and determine if bison seropositive for antibody against MCF-group viruses are resistant to developing MCF after intranasal challenge. In this study, the minimal infectious dose and minimal lethal dose overlap, suggesting that experimental production of subclinically infected bison is impractical. Dose is inversely related to both incubation period and the period between nebulization and first detection of >1000 OvHV-2 DNA copies/500 ng total DNA in peripheral blood leukocytes. Interestingly, all of the bison seropositive for anti-MCF-group viral antibody prior to inoculation died of MCF after nebulization. We conclude that previous exposure to an MCF-group virus does not necessarily provide resistance to OvHV-2-induced MCF in bison.
Assuntos
Bison , Infecções por Herpesviridae/veterinária , Herpesviridae/isolamento & purificação , Febre Catarral Maligna/transmissão , Animais , Febre Catarral Maligna/virologia , Muco , Nariz , Ovinos , Doenças dos Ovinos/virologiaRESUMO
Ovine herpesvirus 2 (OvHV-2) is the causative agent of sheep-associated malignant catarrhal fever (SA-MCF), a frequently fatal disease of some members of the order Artiodactyla. OvHV-2 is carried as a lifelong subclinical infection in sheep (Ovis aries). To date OvHV-2 has not been propagated in vitro and this has hampered studies of viral pathogenesis and efforts to develop a vaccine to protect animals from SA-MCF. Lytic OvHV-2 replication occurs in the lungs of experimentally infected sheep at early times post-inoculation (PI) and in the nasal cavities of naturally infected sheep during virus shedding episodes. Identification of specific cell types supporting lytic virus replication in vivo provides information that can be used in the development of an in vitro propagation system for the virus. Using fluorescence immunohistochemical techniques, we identified lytically infected alveolar epithelial cells in the lungs of sheep early during infection. Lytically infected epithelial cells were also detected in samples of nasal secretions collected from naturally infected sheep during episodes of virus shedding. This is the first reported identification in the natural reservoir species of specific cell types that support OvHV-2 lytic replication in vivo.
Assuntos
Gammaherpesvirinae/fisiologia , Febre Catarral Maligna/virologia , Mucosa Respiratória/virologia , Ovinos/virologia , Animais , Anticorpos Antivirais/imunologia , Proteínas do Capsídeo/imunologia , Gammaherpesvirinae/imunologia , Pulmão/virologia , Mucosa Nasal/virologia , Alvéolos Pulmonares/virologia , Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária , Doenças dos Ovinos/virologiaRESUMO
Sheep-associated malignant catarrhal fever (SA-MCF) caused by ovine herpesvirus-2 (OvHV-2), a gamma-herpesvirus in the Macavirus genus, is a fatal disease associated with lymphoproliferation, lymphocytic vasculitis, and mucosal ulceration in clinically susceptible species. SA-MCF is an important threat to American bison (Bison bison) due to their high susceptibility to this disease. Currently, the pathogenesis of disease in SA-MCF is poorly understood, and the immunophenotype of lymphocytes that infiltrate the vascular lesions of bison and cattle with SA-MCF has been only partially defined. Previous single-color immunohistochemistry studies have demonstrated that CD8(+) cells and CD4(+) cells predominate within vascular infiltrates in cattle and bison. The CD8(+) cells detected in the vascular lesions of cattle and bison were assumed to be cytotoxic alphabeta T lymphocytes. However, polychromatic immunophenotyping analyses in this study showed that CD8(+)/perforin(+) gammadelta T cells, CD4(+)/perforin(-) alphabeta T cells, and B cells infiltrate vascular lesions in the urinary bladder, kidney, and liver of six bison with experimentally-induced SA-MCF. CD8(+) alphabeta T cells and WC1(+) gammadelta T cell cells were only infrequently and inconsistently identified. This study confirmed our hypothesis that the predominant CD8(+) lymphocytes infiltrating the vascular lesions of bison with SA-MCF are cytotoxic lymphocytes of the innate immune system, not CD8(+) alphabeta T cells. Results of the present study support the previous suggestions that MCF is fundamentally a disease of immune dysregulation.